48 results on '"Hong, Die"'
Search Results
2. Water-solubility croconic acid-bisindole dye with morpholine ring for tumor NIRF/PA imaging and photothermal therapy activated by lysosome pH-response
- Author
-
You, Pei-Dan, Ouyang, Cheng-Ren, Lu, Fei, Zeng, Chao, Cai, Hong-Die, Shi, Guo-Sheng, Liu, Lihong, and Zhou, Chun-Qiong
- Published
- 2023
- Full Text
- View/download PDF
3. Combination of mulberry leaf active components possessed synergetic effect on SD rats with diabetic nephropathy by mediating metabolism, Wnt/β-catenin and TGF-β/Smads signaling pathway
- Author
-
Ji, Tao, Wang, Juan, Xu, Zhuo, Cai, Hong-Die, Su, Shu-lan, Peng, Xin, and Ruan, Hong-Sheng
- Published
- 2022
- Full Text
- View/download PDF
4. Risk factors and incidence of third trimester stillbirths in China
- Author
-
Hu, Ying, Wu, Qi, Liu, Jia, Hong, Die, Zou, Yuqing, Lu, Jingjing, Wang, Yuhui, Chen, Danqing, Qi, Lu, and Liang, Zhaoxia
- Published
- 2021
- Full Text
- View/download PDF
5. Differences in Root Endophytic Bacterial Communities of Chinese Cork Oak (Quercus variabilis) Seedlings in Different Growth Years
- Author
-
Sha, Weilai, primary, Hong, Die, additional, Che, Yuying, additional, Xue, Yafei, additional, Kong, Yong, additional, Yi, Xianfeng, additional, Zhou, Jing, additional, Yu, Guohong, additional, and Liu, Baoxuan, additional
- Published
- 2023
- Full Text
- View/download PDF
6. P07-035-23 AWeight-Gain-for-Gestational-Age Z-Score Chart for the Assessment of Gestational Weight Gain in Twin Pregnancies in China and Its Association With Preterm Birth
- Author
-
Liang, Zhaoxia, primary, Hu, Ying, additional, Zhu, Shuqi, additional, Liu, Jia, additional, Hong, Die, additional, Wen, Hong, additional, Chen, Lu, additional, Chen, Xixi, additional, Zou, Yuqing, additional, Liu, Jingjing, additional, Chen, Danqing, additional, and Ma, Hao, additional
- Published
- 2023
- Full Text
- View/download PDF
7. Comparative Pharmacokinetics of Nootkatone in a RAT Model of Chronic Kidney Disease VERSUS Normal Controls
- Author
-
Yong-Hui Li, Li, Pei-Pei, Tan, Yin-Feng, Cai, Hong-Die, Zhang, Xiao-Po, Li, You-Bin, and Zhang, Jun-Qing
- Published
- 2019
- Full Text
- View/download PDF
8. Association between maternal gestational weight gain and preterm birth according to body mass index and maternal age in Quzhou, China
- Author
-
Hu, Ying, Wu, Qi, Han, Luyang, Zou, Yuqing, Hong, Die, Liu, Jia, Zhu, Yuying, Zhu, Qiumin, Chen, Danqing, Qi, Lu, and Liang, Zhaoxia
- Published
- 2020
- Full Text
- View/download PDF
9. A Highly Soluble and Readily Accessible Viologen Negolyte for Ph-Neutral Aqueous Organic Redox Flow Batteries
- Author
-
Qu, Kangkang, primary, Liu, Yahua, additional, Hong, Die, additional, Shen, Zhaoxi, additional, Zhang, Xu, additional, Han, Xiaozhao, additional, ran, Jin, additional, and Yang, Zhengjin, additional
- Published
- 2023
- Full Text
- View/download PDF
10. Metabonomic study of the fruits of Alpinia oxyphylla as an effective treatment for chronic renal injury in rats
- Author
-
Li, Yong-hui, Tan, Yin-feng, Cai, Hong-die, and Zhang, Jun-qing
- Published
- 2016
- Full Text
- View/download PDF
11. Differential systemic exposure to galangin after oral and intravenous administration to rats
- Author
-
Chen, Feng, Tan, Yin-Feng, Li, Hai-Long, Qin, Zhen-Miao, Cai, Hong-Die, Lai, Wei-Yong, Zhang, Xiao-Po, Li, Yong-Hui, Guan, Wei-Wei, Li, You-Bin, and Zhang, Jun-Qing
- Published
- 2015
- Full Text
- View/download PDF
12. Combination of mulberry leaf active components possessed synergetic effect on SD rats with diabetic nephropathy by mediating metabolism, Wnt/β-catenin and TGF-β/Smads signaling pathway
- Author
-
Tao Ji, Juan Wang, Zhuo Xu, Hong-Die Cai, Shu-lan Su, Xin Peng, and Hong-Sheng Ruan
- Subjects
Pharmacology ,Male ,Sphingolipids ,Arachidonic Acid ,Rats ,Plant Leaves ,Rats, Sprague-Dawley ,Alkaloids ,Transforming Growth Factor beta ,Drug Discovery ,Diabetes Mellitus ,Animals ,Humans ,Diabetic Nephropathies ,Female ,Morus ,beta Catenin ,Signal Transduction - Abstract
Mulberry leaf has attracted much attention due to its excellent curative effect on diabetes and its complications, whether the combination of its effective components have protective and synergistic effect on diabetic nephropathy (DN) in vivo remain unclear.The aim of this study was to investigate the protective and synergistic effect of the combination (MAFA step by step method consisted of network pharmacological prediction, animal in vivo validation and metabolic mechanism research was used to construct the multi-component-target-pathway network of mulberry leaf against DN. Firstly, the potential components and mechanism of mulberry leaf against DN was explored by network pharmacology analysis. Secondly, DN animal model was established to validate the anti-DN activity of these potential compounds. Thirdly, the metabolomics of serum and urine samples from animal experiments was analyzed to explore the anti-DN mechanism of these potential compounds.The results of network pharmacology demonstrated that a total of 7 compounds detected in MA and MF exhibited anti-DN activity, their mechanism were strongly in connection with metabolic pathways, arachidonic acid metabolism, sphingolipid signaling pathway, etc. The results of animal experiment indicated that MAFMAF
- Published
- 2021
13. Comparative Pharmacokinetics of Nootkatone in a RAT Model of Chronic Kidney Disease VERSUS Normal Controls
- Author
-
Xiaopo Zhang, Yinfeng Tan, Pei-Pei Li, Li Yonghui, Youbin Li, Hong-die Cai, and Junqing Zhang
- Subjects
Electrospray ,Chromatography ,Formic acid ,010401 analytical chemistry ,Cmax ,010402 general chemistry ,Mass spectrometry ,01 natural sciences ,High-performance liquid chromatography ,0104 chemical sciences ,Analytical Chemistry ,chemistry.chemical_compound ,chemistry ,Pharmacokinetics ,Nootkatone ,Sample preparation - Abstract
In this study, a simple, sensitive, and rapid analytical method was developed by ultra-performance liquid chromatography (UPLC) coupled with tandem quadrupole mass spectrometry (MS) for the determination of nootkatone in normal and chronic kidney disease (CKD) rat plasma using clarithromycin as internal standard. After sample preparation by simple liquid–liquid extraction, chromatography was performed on an Acquity UPLC BEH C18 column (2.1 × 50 mm, 1.7 mm particle size) using gradient elution with the mobile phase composed of acetonitrile and water acidified with 0.1% (v/v) formic acid. Detection was achieved by electrospray ionisation MS under the multiple selective reaction monitoring mode. The linear range was 0.01‒500 ng/mL with the square regression coefficient (r) of 0.9975. The lower limit of quantification was 0.01 ng/mL. The intra- and inter-day precision was under 5% and the stability accuracy was between 3.6 and 7.0%. The average recoveries from spiked plasma samples were >83% and matrix effect was over 81%. The developed method was successfully applied to the pharmacokinetic study of nootkatone in normal and CKD rats after an oral administration of 50 mg/kg nootkaone. The results showed the cmax and area under curve of nootkaone were greatly decreased, meanwhile Vd/F and t1/2 were markedly increased in CKD rats. The pharmacokinetic characteristics of nootkatone in rats were significantly altered in CKD rats.
- Published
- 2019
14. Danshen can interact with intestinal bacteria from normal and chronic renal failure rats
- Author
-
Zhenhua Zhu, Dawei Qian, Sheng Guo, Jianming Guo, Jin-Ao Duan, Hong-Die Cai, Li Yonghui, Yue Zhu, and Shulan Su
- Subjects
Male ,0301 basic medicine ,Glucuronidation ,Salvia miltiorrhiza ,Intestinal bacteria ,RM1-950 ,Pharmacology ,Salviae miltiorrhizae radix et rhizoma ,Peptostreptococcaceae ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,Clostridium ,Butyrivibrio ,Chronic renal failure ,Animals ,Demethylation ,biology ,Chemistry ,Ruminococcus ,Pseudomonas ,General Medicine ,biology.organism_classification ,Gastrointestinal Microbiome ,Rats ,030104 developmental biology ,030220 oncology & carcinogenesis ,Salvianolic acid ,Tanshinone ,Kidney Failure, Chronic ,Peptococcus ,Therapeutics. Pharmacology ,Biomarkers - Abstract
Danshen (Salviae Miltiorrhizae Radix et Rhizoma, SMR) has been used as a traditional Chinese medicine in clinic for treatment of coronary heart diseases. Previous works have shown that the chronic renal failure (CRF) is closely related to changes of intestinal bacteria. The aim is to explore the interaction between active components of SMR and intestinal bacteria from normal and CRF rats. The changes of intestinal bacteria were evaluated among normal rats, CRF model rats and SMR-treated rats via 16S rRNA gene sequencing technology. UPLC-QTOF/MS was applied for the analysis and identification of metabolites. RESULTS: Results showed that the following intestinal bacteria varied significantly in CRF rats, including Mucispirillum, Kurthia, Clostridium, Blautia, Butyrivibrio, Shuttleworthia, Peptococcus, Ruminococcus, Bradyrhizobium, Methylobacterium, Azospirillum, Thalassospira, Methylophilus, Pseudomonas, peptostreptococcaceae and bacteroidales. The ethanol extract of SMR (DS) significantly regulated Shuttleworthia, peptostreptococcaceae and Pseudomonas, while the water extract (DSS) significantly affected Peptococcus, peptostreptococcaceae and Ruminococcus. Methylation, demethylation, dehydrogenation, hydrogenation and hydroxylation were the major metabolic transformation of tanshinones in vitro by intestinal bacteria. Glucuronidation, methylation and hydrogenation were the main metabolic transformation of salvianolic acids. These results showed that the bioactive components of SMR, including tanshinones and salvianolic acids, might exert the medical effect via regulation intestinal bacteria.
- Published
- 2019
15. Salvia miltiorrhiza protects against diabetic nephropathy through metabolome regulation and wnt/β-catenin and TGF-β signaling inhibition
- Author
-
Xinxin Dai, Dawei Qian, Jianming Guo, Yue Zhu, Hong-Die Cai, Hui Yan, Xiang Xiang, Zhi-Shu Tang, Sheng Guo, Shulan Su, Wei Gu, and Jin-Ao Duan
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,medicine.medical_treatment ,Glomerular Mesangial Cell ,Intraperitoneal injection ,Blood sugar ,Salvia miltiorrhiza ,Kidney ,Protective Agents ,Rats, Sprague-Dawley ,Diabetic nephropathy ,03 medical and health sciences ,0302 clinical medicine ,Transforming Growth Factor beta ,Internal medicine ,Diabetes mellitus ,medicine ,Metabolome ,Animals ,Diabetic Nephropathies ,beta Catenin ,Pharmacology ,Plant Extracts ,Chemistry ,medicine.disease ,Wnt Proteins ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,030220 oncology & carcinogenesis ,Signal Transduction - Abstract
Diabetic nephropathy (DN) is a complication of diabetes that is caused by uncontrolled high blood sugar. It has been reported that Salvia miltiorrhiza (SM) possesses the ability to prevent kidney damage, although the mechanisms remain unclear. The study was to investigate whether and how SM improved DN injury via regulation of metabolome and the molecular mechanisms. In this study, SD rats were fed a high glucose / high fat diet accompanied by 0.5% glucose water. Three weeks later, the rats were given one intraperitoneal injection of 30 mg/kg STZ each day for three days for DN model. The biochemical indicators and metabolomics of plasma, urine and renal tissue were analyzed. Then the western blotting analysis of renal tissue and glomerular mesangial cells were investigated. The results showed that Salvia miltiorrhiza extracts improved the renal injury and regulation of abnormal glycolipid metabolism. The metabolites in serum, urine and renal tissues have been changed significantly. The involved metabolic pathways mainly include phospholipid, arachidonic acid, and pyrimidine metabolisms. Meanwhile, SM inhibited the relative expression levels of wnt4, β-catenin and TGF-β in renal tissue and high-glucose induced glomerular mesangial cells.
- Published
- 2019
16. Simultaneous Determination of Four Tanshinones by UPLC-TQ/MS and Their Pharmacokinetic Application after Administration of Single Ethanol Extract of Danshen Combined with Water Extract in Normal and Adenine-Induced Chronic Renal Failure Rats
- Author
-
Hong-Die Cai, Shu-Lan Su, Yonghui Li, Zhenhua Zhu, Jianming Guo, Yue Zhu, Sheng Guo, Dawei Qian, and Jinao Duan
- Subjects
Salvia miltiorrhiza Bge. ,tanshinones ,pharmacokinetics ,UPLC-TQ/MS ,chronic renal failure ,Organic chemistry ,QD241-441 - Abstract
Salvia miltiorrhiza, one of the major traditional Chinese medicines, is commonly used and the main active ingredients—tanshinones—possess the ability to improve renal function. In this paper, the UPLC-TQ/MS method of simultaneously determining four tanshinones—tanshinone IIA, dihydrotanshinone I, tanshinone I, and cryptotanshinone—was established and applied to assess the pharmacokinetics in normal and chronic renal failure (CRF) rat plasma. The pharmacokinetics of tanshinones in rats were studied after separately intragastric administration of Salvia miltiorrhiza ethanol extract (SMEE) (0.65 g/kg), SMEE (0.65 g/kg) combined with Salvia miltiorrhiza water extract (SMWE) (1.55 g/kg). The results showed Cmax and AUC0–t of tanshinone IIA, tanshinone I, cryptotanshinone reduced by 50%~80% and CLz/F increased by 2~4 times (p < 0.05) in model group after administrated with SMEE. Nevertheless, after intragastric administration of a combination of SMWE and SMEE, the Cmax and AUC0–t of four tanshinones were upregulated and CLz/F was downregulated, which undulated similarity from the model group to the normal group with compatibility of SMEE and SMWE. These results hinted that SMWE could improve the bioavailability of tanshinones in CRF rats, which provides scientific information for further exploration the mechanism of the combination of SMWE and SMEE and offers a reference for clinical administration of Salvia miltiorrhiza.
- Published
- 2016
- Full Text
- View/download PDF
17. [Effect of Salviae Miltiorrhizae Radix et Rhizoma on diversity of intestinal flora in diabetic nephropathy rats]
- Author
-
Hong-Die, Cai, Shu-Lan, Su, Jian-Ming, Guo, and Jin-Ao, Duan
- Subjects
Animals ,Diabetic Nephropathies ,Salvia miltiorrhiza ,Diabetes Mellitus, Experimental ,Drugs, Chinese Herbal ,Gastrointestinal Microbiome ,Rats - Abstract
This study aimed to explore the effect of Salviae Miltiorrhizae Radix et Rhizoma, its stems and leaves on the diversity of intestinal microflora in rats with diabetic kidney injury. Diabetic rats model was established by feeding high glucose and high fat diet and 5% glucose solution with intraperitoneal injection of 30 mg·kg~(-1) streptozocin(STZ). The rats were randomly divided into normal group, model group, irbesartan control group, Huangkui Capsules control group, as well as low, middle and high dose groups of Sal-viae Miltiorrhizae Radix et Rhizoma, its stems and leaves. After administration for 2 weeks, 16 S rRNA technique was used to analyze the diversity of intestinal microflora in the feces of each group. The results showed rats in the model group developed renal tubular epithelial vacuole degeneration and a large amount of inflammatory cell infiltration in the renal interstitium. A small amount of inflammatory cell infiltration was seen in each administration group. The kidney structure of rats in irbesartan group, Huangkui Capsules group, high-dose group of Salviae Miltiorrhizae Radix et Rhizoma and its stem water extract, as well as high dose group of Salviae Miltiorrhizae Radix et Rhizoma and its stem ethnol extract group was close to the normal group. The diversity and structure of intestinal flora in the model group were significantly different from those in the normal group. Each administration group improved the fecal flora diversity in rats with diabetic kidney injury to a certain extent, especially the high dose of Salviae Miltiorrhizae Radix et Rhizoma and its stems water extract. Different flora were found in feces of diabetic nephropathy model rats on class, order, family and genus levels. On families and genera levels, the relative abundance of Bifidobacterium, Turicibacter, Peptostreptococcaceae, Desulfovibrio, and SMB53 showed an upward trend in model group, but that of Lactobacillus, Clostridium, Rikenella, Rumen fungi showed a downward trend. The administration groups can improve the relative abundance of the above intestinal flora in the model rats to a normal-like level. The results of this study provide a reference for resource utilization and further development of Salviae Miltiorrhizae Radix et Rhizoma.
- Published
- 2021
18. [Diagnosis and Treatment of Primary Aldosteronism in West China Hospital of Sichuan University from 2009 to 2018]
- Author
-
Hong-Die, Liu, Yuan-Mei, Li, Wen, Wang, Qian-Rui, Li, Tao, Chen, Yu-Chun, Zhu, Yan, Ren, and Hao-Ming, Tian
- Subjects
Male ,China ,Universities ,Hyperaldosteronism ,Hypertension ,Adrenal Gland Neoplasms ,Humans ,Female ,Aldosterone - Abstract
To summary the clinical diagnosis and treatment of primary aldosteronism (PA) in West China Hospital (WCH) of Sichuan University during 2009-2018.This study enrolled the patients diagnosed as PA and admitted in WCH of Sichuan University from January 2009 to December 2018. The information of the patients including epidemiological and clinical data, diagnosis and treatment as well as therapeutic outcomes were collected and analyzed.A total of 853 patients with 1 248 diagnostic cases were included in the analysis, and the diagnosis cases of PA increased year by year from 2009 to 2018. Most patients (74.33%) were confirmed the diagnosis in the Department of Endocrinology and Metabolism and then admitted to the hospital. PA was more frequent in female than in male, with a ratio of female to male about 1.34∶1. Hypertension was the most common chief complaint, in contrast, the proportion of fatigue and/or numbness as the symptoms of hypokalemia was declining. More and more patients were diagnosed because of imaging examination founding adrenal incidentoma. After 2016, more and more patients were diagnosed by recumbent saline suppression test and captopril challenge test, and the number of adrenal venous sampling to classify PA subtypes was increasing to help choosing different treatment options. The proportion of surgical treatment decreased year by year, and more and more patients adopted medical treatment or transferred to surgery with combined treatment instead of simple operation.During the past 10 years, remarkable progress was made in the diagnosis and treatment of PA. Hypertension was the most important clinical manifestation of PA, so the screening of PA in hypertensive patients should be strengthened. Adrenal incidentaloma has become prevalent manifestation of PA with an increasing trend, which needs more attention in clinical practice.
- Published
- 2020
19. Renal protective effect and action mechanism of Huangkui capsule and its main five flavonoids
- Author
-
Dawei Qian, Jin-Ao Duan, Tang Renmao, Xu Dong Cong, Sheng Guo, Shulan Su, Hong-Die Cai, and Weiwei Tao
- Subjects
Male ,0301 basic medicine ,Epithelial-Mesenchymal Transition ,Pharmacology ,Kidney ,Nephropathy ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Western blot ,Abelmoschus ,Drug Discovery ,medicine ,Animals ,Medicine, Chinese Traditional ,Flavonoids ,chemistry.chemical_classification ,Creatinine ,Reactive oxygen species ,NADPH oxidase ,medicine.diagnostic_test ,biology ,Plant Extracts ,Kinase ,NADPH Oxidase 1 ,medicine.disease ,Rats ,030104 developmental biology ,chemistry ,030220 oncology & carcinogenesis ,biology.protein ,Kidney Failure, Chronic ,Quercetin - Abstract
Ethnopharmacological relevance The flower of Abelmoschus manihot (Linn.) Medicus ( A. manihot), as a traditional Chinese Herbal medicine, was used widely in China with efficacy of inducing diuresis for treating strangurtia, and subdhing swelling and detoxicating. It has been reported that Huangkui capsule, prepared by the extract of the flower of A. manihot , can reduce the content of urinary protein, serum creatinine and serum urea nitrogen in nephropathy rats and processes renoprotective activity, while the action mechanism need to illuminate deeply. Aims of the study In this study, we investigated the protection effect of Huangkui capsule on tubulointerstitial fibrosis in chronic renal failure (CRF) rats and its mechanism against high glucose-induced epithelial to mesenchymal transition (EMT) in renal tubular epithelial cells (HK-2) of its bioactive components. Materials and methods The animals were divided into normal group, CRF model group and Huangkui capsule-treated group. Hematoxylin eosin (HE) staining and Masson staining were applied to observe pathological changes in renal tissue of different groups. Biochemical indicators including serum urea nitrogen (BUN), urine protein (UP) and serum creatinine (Scr) were measured according to the manufacturer's instructions of kits. HK-2 cell damaged model was established to access the protection effect and action mechanism of five main flavonoids from Huangkui capsule. The experimental cells were divided into eight groups: control group, model group, positive drug group and five main flavonoids treated groups. The dichlorodihydrofluorescein diacetate (DCFH-DA) assay was used to determine the reactive oxygen species (ROS) in different groups. Western blot was applied to analyze the expression of pathogenesis-related proteins in different groups. Results The results stated that Huangkui capsule significantly inhibited the elevation of Scr, BUN, UP, the expression of α-smooth muscle actin (α-SMA), phosphorylation-extracellular signal-regulated kinase (p-ERK1/2), NADPH Oxidase 1, NADPH Oxidase 2 and NADPH Oxidase 4 in adenine-induced CRF rats. The main bioactive components of quercetin (QT), hyperoside (HY), isoquercitrin (IQT), gossypetin-8- O - β -D-glucuronide (GG) and quercetin-3′- O -glucoside (QG) at the dosage of 100 µM, like NADPH oxidase inhibitor diphenyleneiodonium, exhibited a significant effect on inhibiting the expression of α-SMA, p-ERK1/2, NADPH Oxidase 1, NADPH Oxidase 2 and NADPH Oxidase 4 in high glucose-induced HK-2 cells, especially GG. Conclusions These results demonstrated that Huangkui capsule and the flavonoids components prevent tubulointerstitial fibrosis in CRF rat involvement in the action mechanism of inhibiting NADPH oxidase/ROS/ERK pathway.
- Published
- 2017
20. Diagnosis and treatment for hyperuricemia and gout: a systematic review of clinical practice guidelines and consensus statements
- Author
-
Anoop D. Shah, Yaolong Chen, Qianrui Li, Hao Chen, Haoming Tian, Ling Li, Zhenmei An, Sheng-Chia Chung, Harry Hemingway, Xin Sun, Jing Wang, Joey Sum-Wing Kwong, Hong-Die Liu, Xiaodan Li, and Sheyu Li
- Subjects
medicine.medical_specialty ,Consensus ,Gout ,lcsh:Medicine ,hyperuricemia ,Gout Suppressants ,03 medical and health sciences ,0302 clinical medicine ,systematic review ,Humans ,Medicine ,Agree ii ,030212 general & internal medicine ,Hyperuricemia ,Methodological quality ,business.industry ,Quality assessment ,Research ,lcsh:R ,General Medicine ,Guideline ,medicine.disease ,Uric Acid ,3. Good health ,Clinical Practice ,Diabetes and Endocrinology ,Data extraction ,Family medicine ,Practice Guidelines as Topic ,business ,clinical practice guideline ,030217 neurology & neurosurgery - Abstract
ObjectivesDespite the publication of hundreds of trials on gout and hyperuricemia, management of these conditions remains suboptimal. We aimed to assess the quality and consistency of guidance documents for gout and hyperuricemia.DesignSystematic review and quality assessment using the appraisal of guidelines for research and evaluation (AGREE) II methodology.Data sourcesPubMed and EMBASE (27 October 2016), two Chinese academic databases, eight guideline databases, and Google and Google scholar (July 2017).Eligibility criteriaWe included the latest version of international and national/regional clinical practice guidelines and consensus statements for diagnosis and/or treatment of hyperuricemia and gout, published in English or Chinese.Data extraction and synthesisTwo reviewers independently screened searched items and extracted data. Four reviewers independently scored documents using AGREE II. Recommendations from all documents were tabulated and visualised in a coloured grid.ResultsTwenty-four guidance documents (16 clinical practice guidelines and 8 consensus statements) published between 2003 and 2017 were included. Included documents performed well in the domains of scope and purpose (median 85.4%, range 66.7%–100.0%) and clarity of presentation (median 79.2%, range 48.6%–98.6%), but unsatisfactory in applicability (median 10.9%, range 0.0%–66.7%) and editorial independence (median 28.1%, range 0.0%–83.3%). The 2017 British Society of Rheumatology guideline received the highest scores. Recommendations were concordant on the target serum uric acid level for long-term control, on some indications for urate-lowering therapy (ULT), and on the first-line drugs for ULT and for acute attack. Substantially inconsistent recommendations were provided for many items, especially for the timing of initiation of ULT and for treatment for asymptomatic hyperuricemia.ConclusionsMethodological quality needs improvement in guidance documents on gout and hyperuricemia. Evidence for certain clinical questions is lacking, despite numerous trials in this field. Promoting standard guidance development methods and synthesising high-quality clinical evidence are potential approaches to reduce recommendation inconsistencies.PROSPERO registration numberCRD42016046104.
- Published
- 2019
- Full Text
- View/download PDF
21. Protective Effects of Total Glycoside From Rehmannia glutinosa Leaves on Diabetic Nephropathy Rats via Regulating the Metabolic Profiling and Modulating the TGF-β1 and Wnt/β-Catenin Signaling Pathway
- Author
-
Dawei Qian, Erxin Shang, Hong-Die Cai, Xinxin Dai, Tianyao Zheng, Zhenhua Zhu, Jin-Ao Duan, Hui Yan, Shulan Su, Sheng Guo, and Dandan Wei
- Subjects
0301 basic medicine ,total glycoside extracted from leaves of Rehmannia ,Wnt/β-catenin signaling pathway ,Glucuronate ,medicine.medical_treatment ,Intraperitoneal injection ,Endogeny ,Pharmacology ,Diabetic nephropathy ,03 medical and health sciences ,0302 clinical medicine ,TGF-β1 ,medicine ,Pharmacology (medical) ,Kidney ,biology ,Chemistry ,diabetic nephropathy ,lcsh:RM1-950 ,Wnt signaling pathway ,Streptozotocin ,Rehmannia glutinosa ,biology.organism_classification ,medicine.disease ,metabolomics ,030104 developmental biology ,medicine.anatomical_structure ,lcsh:Therapeutics. Pharmacology ,030220 oncology & carcinogenesis ,medicine.drug ,Dihuangye total glycoside capsule - Abstract
Rehmannia glutinosa Libosch (RG), is officially listed in the Chinese Pharmacopoeia and is widely used in China. The leaves of RG (LR) is an important vegetative organ of the plant. At present, the total glycosides of RG (TLR) were extracted from RG, and developed a national second class of new drugs to the Dihuangye total glycoside capsule (DTG). Additionally, DTG has the effect of nourishing yin and tonifying kidney, promoting blood circulation and blood cooling, and applicable to chronic glomerulonephritis mild to Qi and Yin Deficiency. Moreover, diabetic nephropathy (DN) rats model was induced by intraperitoneal injection of a small dose of streptozotocin (45 mg/kg) and high-fat diet and plus 5% glucose drinking water. Over 15 days, after oral administration TLR and DTG in DN rats, samples from serum, urine and kidney were collected for biochemical indicators measurements, pathological analysis, western blotting and metabolomics. Therefore, the analytical results of biochemical indicators, histopathological observations and western blotting showed that TLR and DTG exhibited a significant effect in renal protection. And 27 endogenous metabolites (12 in serum and 15 in urine) could be tentatively identified in the process of DN in rats using metabolomics method. Those endogenous metabolites were chiefly involved in sphingolipid metabolism; pentose, glucuronate interconversion; terpenoid backbone biosynthesis; purine metabolism and retinol metabolism. After drug intervention, these endogenous metabolites turned back to normal level some extent (P < 0.05). Furthermore, TLR and DTG prevent high glucose-induced glomerular mesangial cells (GMCs) by inhibiting TGF-β1 and Wnt/β-catenin signaling pathway, providing a powerful supports to develop a new therapeutic agent for DN. This study paved the way for further exploration of the pathogenesis of DN, early diagnosis and the evaluation of curative effect.
- Published
- 2018
22. [Antidepressant activity of flavonoid ethanol extract of Abelmoschus manihot corolla with BDNF up-regulation in the hippocampus]
- Author
-
Hong-die, Cai, Wei-wei, Tao, Shu-lan, Su, Sheng, Guo, Yue, Zhu, Jian-ming, Guo, Da-wei, Qian, Xu-dong, Cong, Ren-mao, Tang, and Jin-ao, Duan
- Subjects
Flavonoids ,China ,Depressive Disorder, Major ,Ethanol ,Plant Extracts ,Brain-Derived Neurotrophic Factor ,Hippocampus ,Antidepressive Agents ,Up-Regulation ,Mice ,Hindlimb Suspension ,Abelmoschus ,Animals ,Quercetin ,Swimming ,Drugs, Chinese Herbal - Abstract
Abelmoschus manihot (L.) Medic., a folk herbal medicine in China, is a flowering plant belonging to Abelmoschus L. genus and Malvaceae family, which has been reported with an antidepressant activity. The study was designed to isolate flavonoids from Abelmoschus manihot corolla and explore the action mechanism of antidepressant activities. The flavonoids were isolated and purified by D101 macroporous resin column, polyamide column and Sephadex LH-20 sequentially and identified as myricetin-3-O-β-D-glucoside (1), gossypetin-8-O-β-D-glucuronide (2, G-8-G), gossypetin-3'-O-β-D-glucoside (3), quercetin-3'-glucoside (4, Q-3-G), isoquercitrin (5, IQT), hyperoside (6, HY), myricetin (7), quercetin (8, QT). Compounds 2, 4, 5, 6 and 8 (15, 30 and 60 mg·kg−1) were orally administered to mice and the reaction was observed in tail suspension test (TST) and forced swimming test (FST). Western blot analysis was used in determination of the protein expressions of brain-derived neurotrophic factor (BDNF), tyrosine receptor kinase B (TrkB) and phosphorylation eukaryotic elongation factor 2 (p-eEF2). The results revealed that only Q-3-G and G-8-G (15, 30, 60 mg ·kg−1) significantly reduced the immobility time in FST and TST. Furthermore, Q-3-G and G-8-G remarkably increased the expression of BDNF and TrkB, and decreased the expression of p-eEF2. These results suggest that Q-3-G and G-8-G had an obvious antidepressant activity via up-regulation of BDNF expression. The new observation will provide a new direction in the development of antidepressant in the treatment of major depressive disorder (MDD).
- Published
- 2018
23. Salvia miltiorrhiza protects against diabetic nephropathy through metabolome regulation and wnt/β-catenin and TGF-β signaling inhibition
- Author
-
Xiang, Xiang, primary, Cai, Hong-Die, additional, Su, Shu-lan, additional, Dai, Xin-xin, additional, Zhu, Yue, additional, Guo, Jian-ming, additional, Yan, Hui, additional, Guo, Sheng, additional, Gu, Wei, additional, Qian, Da-wei, additional, Tang, Zhi-shu, additional, and Duan, Jin-ao, additional
- Published
- 2019
- Full Text
- View/download PDF
24. Determination of α-hederin in rat plasma using liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and its application to a pharmacokinetic study
- Author
-
Yu Chao Luo, Feng Chen, Hai Long Li, Yin Feng Tan, Hong Die Cai, Zhen Miao Qin, Bei Li, Jun Qing Zhang, and Xiao Po Zhang
- Subjects
Volume of distribution ,Analyte ,Chromatography ,General Chemical Engineering ,Selected reaction monitoring ,General Engineering ,Ethyl acetate ,Intestinal absorption ,Analytical Chemistry ,Bioavailability ,Hederagenin ,chemistry.chemical_compound ,chemistry ,Pharmacokinetics - Abstract
The evaluation of efficacy and safety should be paralleled with the assessment of comprehensive pharmacokinetic (PK) properties for a drug candidate, and a robust bioanalytical method is a prerequisite for obtaining PK information. α-Hederin is reported to have various in vitro and in vivo activities; however, very little is known about its PK and metabolic characteristics. In this study, we have developed an efficient LC-ESI(−)-MS/MS assay for α-hederin and its sapogenin hederagenin in rat plasma. Sample cleanup involved methanol precipitation for identification analysis and liquid–liquid extraction with ethyl acetate for quantification assay. LC analysis was performed under reversed-phase conditions in the modified “pulse gradient elution” mode. Analyte identification and quantification were conducted using multiple reaction monitoring (MRM) mode with euscaphic acid as the internal standard. Under these conditions, deglycosylated metabolites and their sulfate conjugates were detected; however, hederagenin was not detected in rat plasma samples after both oral and intravenous treatments. The mean plasma clearance (CL), volume of distribution (VSS) and elimination half-life (t1/2) of α-hederin were 0.24 L h−1 kg−1, 0.25 L kg−1 and 2.67 h, respectively. The oral bioavailability (F) of α-hederin in rats was about 0.14%, which might result from the poor intestinal absorption and/or extensive biliary excretion. It is hoped that this validated method will be useful for future PK studies of α-hederin.
- Published
- 2015
25. Protective effects of Salvia miltiorrhiza on adenine-induced chronic renal failure by regulating the metabolic profiling and modulating the NADPH oxidase/ROS/ERK and TGF-β/Smad signaling pathways
- Author
-
Li Yu, Huiting Zeng, Dawei Qian, Yuping Tang, Yue Zhu, Jin-Ao Duan, Zhenhua Zhu, Li Yonghui, Sheng Guo, Jianming Guo, Hong-Die Cai, and Shulan Su
- Subjects
0301 basic medicine ,Male ,medicine.medical_specialty ,Renal function ,Salvia miltiorrhiza ,Smad Proteins ,medicine.disease_cause ,Cell Line ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Transforming Growth Factor beta ,Internal medicine ,Drug Discovery ,medicine ,Animals ,Humans ,Extracellular Signal-Regulated MAP Kinases ,Blood urea nitrogen ,Pharmacology ,chemistry.chemical_classification ,Kidney ,Reactive oxygen species ,Creatinine ,NADPH oxidase ,biology ,Chemistry ,Plant Extracts ,Adenine ,NADPH Oxidases ,Rats ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Gene Expression Regulation ,030220 oncology & carcinogenesis ,biology.protein ,Kidney Failure, Chronic ,Reactive Oxygen Species ,Oxidative stress ,Biomarkers ,Phytotherapy - Abstract
Ethnopharmacological relevance Chronic renal failure (CRF) is defined as a progressive and irreversible loss of renal function and associated with inflammation and oxidative stress. Salvia miltiorrhiza (SM) is an important Chinese herb used in traditional Chinese medicine for treating cardiovascular diseases. The previous studies showed the SM exhibited significant protective effects on CRF. In this present study, the metabolic profiling changes and action mechanism of SM on CRF were explored. Aims of the study The aims of this study were to illustrate the metabolic profiling changes of adenine induced CRF and analyze the protective effects and action mechanisms of SM ethanol extract (SMEE) and water extract (SMWE). Materials and methods The animals were divided into normal group, CRF model group, Huangkui capsule-treated group, SMEE-treated group and SMWE-treated group. The UPLC−QTOFMS coupled with multivariate statistical methods were used to explore the changes of metabolic profile in plasma, urine and renal tissue from CRF rats simultaneously after treatment with SMEE and SMWE. Hematoxylin eosin (HE) staining and Masson staining were applied to observe pathological changes in renal tissue. Biochemical indicators including serum urea nitrogen (BUN), urine protein (UP) and serum creatinine (Scr) were measured according to the manufacturer's instructions of kits. Furthermore, HK-2 cell damaged model induced by ISF was established to access the protective effects and action mechanism. The dichlorodihydrofluorescein diacetate (DCFH-DA) assay was used to determine the reactive oxygen species (ROS) and Western blot was applied to analyze the expression of pathogenesis-related proteins in different groups. Results The results showed that the ethanol extract (SMEE) and water extract (SMWE) of SM significantly inhibited the elevation of serum creatinine (Scr), blood urea nitrogen (BUN), urine protein (UP) and indoxyl sulfate (ISF) in adenine-induced CRF rats, especially SMEE exhibited more significant effects. Moreover, SM extracts obviously improved the symptoms of glomerular and tubular atrophy, focal calcium deposits, interstitial fibrosis, interstitial inflammation, and renal tissues. By metabolomics analysis, fifty-nine metabolites (thirteen in plasma, twenty-seven in urine and nineteen in kidney tissue) were up-regulated or down-regulated and contributed to CRF progress. After treatment of SM extracts, the altered metabolites were restored back to normal level. These potential biomarkers underpinning the metabolic pathways are including phenylalanine metabolism, pyrimidine metabolism, purine metabolism and tryptophan metabolism. Furthermore, SM extracts prevent epithelial-mesenchymal transition (EMT) of human renal tubular epithelial (HK-2) cell by inhibiting NADPH oxidase/ROS/ERK and TGF-β/Smad signaling pathways. Conclusions SMEE and SMWE can significantly alleviate adenine-induced CRF via regulation of the metabolic profiling and modulation of NADPH oxidase/ROS/ERK and TGF-β/Smad signaling pathways, which provided important supports for the development of protective agent of SM for CRF.
- Published
- 2017
26. [Silkworm excrement bacterial communities diversity in different instars based on 16S rDNA sequence analysis]
- Author
-
Tian-Yao, Zheng, Shu-Lan, Su, Hong-Die, Cai, Xin-Xin, Dai, Zhen, Ouyang, and Jin-Ao, Duan
- Subjects
DNA, Bacterial ,Feces ,Bacteria ,RNA, Ribosomal, 16S ,Animals ,High-Throughput Nucleotide Sequencing ,Biodiversity ,Sequence Analysis, DNA ,Bombyx ,Phylogeny - Abstract
This paper investigated the diversity of the silkworm excrement bacterial communities in different ages before and after drying, aiming to clarify the differences of bacterial communities in composition and bacterial abundance and the influences of drying treatment, and provide scientific basis for the efficacy of scientific connotation and utilization of silkworm excrement. High-throughput sequencing technique was used to measure the sequence of 16S rDNA-V4 variable region of bacteria in silkworm excrement. QIIME, Mothur and PICRUSt software programs were employed to sort and calculate the number of sequences and operational taxonomic units (OTUs) for each sample. Thereafter, the abundance, distribution, alpha diversity index of species, beta diversity and bacterial communities diversity among different sample groups and predicted the bacterial gene functions were analyzed. In this study, the numbers of effective sequences for six samples were 259 250; the rarefaction curves showed a sufficient sequencing depth, and the number of OTUs was close to saturation. The bacteria in silkworm excrement belonged to the following five phylums: Proteobacteria (89.3%), Actinobacteria (5.0%), Firmicutes (4.4%), Bacteroidetes (1.1%) and Cyanobacteria (0.2%). The dominant specie was Cyanobacteria of the total bacteria identified, respectively. The abundances and diversities of the silkworm excrement bacterial communities have been reduced after drying treatment, especially the silkworm excrement of the fifth instar. PICRUSt analysis was performed to show that abundance of the functional genes such as membrane transport, carbohydrate metabolism, amino acid metabolism, lipid metabolism, nucleotide metabolism, cellular processes and signaling were relatively high. The result showed that the drying treatment could decreased the species and numbers of pathogenic bacteria in silkworm excrement obviously and improve the quality of medicinal materials. Compared with the lower ages, silkworm excrement of fifth instar seems like to be more suitable for use in medicine. Illumina MiSeq high-throughput sequencing system provides a more accurate and scientific data resource for the study of bacteria in silkworm excrement.
- Published
- 2017
27. [Effect of flavonoids from Abelmoschus manihot on proliferation, differentiation of 3T3-L1 preadipocyte and insulin resistance]
- Author
-
Dawei Qian, Jin-Ao Duan, Shulan Su, Yue Zhu, Hong-Die Cai, Weiwei Tao, and Sheng Guo
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Glucose uptake ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Insulin resistance ,Abelmoschus ,Internal medicine ,Adipocyte ,3T3-L1 Cells ,medicine ,Adipocytes ,Animals ,Pharmacology (medical) ,General Pharmacology, Toxicology and Pharmaceutics ,Cell Proliferation ,Flavonoids ,030109 nutrition & dietetics ,Adiponectin ,Chemistry ,food and beverages ,3T3-L1 ,Lipid metabolism ,Cell Differentiation ,medicine.disease ,Endocrinology ,Complementary and alternative medicine ,Resistin ,Insulin Resistance ,Quercetin - Abstract
Abelmoschus manihot was rich in flavonoids, which has been reported the activity on protecting angiocarpy and improving renal function. This study aimed to explore the action mechanism of five flavonoids from A. manihot on how to ameliorating insulin resistance through the regulation of the glucose and expression of PPARγ, C/EBPα, SREBP-1, resistin, visfatin, adiponectin in 3T3-L1 adipocytes. After the 3T3-L1 preadipocytes were differentiated into mature adipocytes, insulin resistance model was built. Insulin resistance adipocytes were treated with 5, 100 μmol•L⁻¹ quercetin, isoquercitrin, hyperoside, quercitrin-3'-O-glucoside, gossypetin-8-O-β-glucoside. The glucose was indirectly determined by BCA kit. The mRNA expression levels of PPARγ, C/EBPα, SREBP-1, resistin, visfatin, adiponectin were detected by real-time quantitative PCR. Results showed that five flavonoids at 5 μmol•L⁻¹ could accelerate preadipocytes proliferation and inhibit that at 100 μmol•L⁻¹ Compared with the normal group, glucose uptake reduced significantly in model group (P
- Published
- 2016
28. Metabonomic study of the fruits of Alpinia oxyphylla as an effective treatment for chronic renal injury in rats
- Author
-
Hong-die Cai, Yinfeng Tan, Junqing Zhang, and Li Yonghui
- Subjects
0301 basic medicine ,Male ,Clinical Biochemistry ,030232 urology & nephrology ,Pharmaceutical Science ,Renal function ,Urine ,Pharmacology ,Analytical Chemistry ,Pathogenesis ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Kynurenic acid ,Drug Discovery ,medicine ,Animals ,Metabolomics ,Spectroscopy ,biology ,Plant Extracts ,Hippuric acid ,Alpinia ,biology.organism_classification ,medicine.disease ,Pathophysiology ,Rats ,030104 developmental biology ,chemistry ,Kidney Failure, Chronic ,Kidney disease - Abstract
Alpinia oxyphylla (Zingiberaceae) is a well-known medicinal plant. Its fruit ("Yi-Zhi-Ren" in Chinese) is used as an anti-diuretic and traditionally used for the treatment of enuresis and reduce urination. Chronic kidney disease (CKD) is a disease with the characteristic of the slowly loss of kidney function and has a prevalence of up to 7-10% in adults. Recent advances in its etiology and pathogenesis are providing more speculative hypotheses focused on integral systems. Using a UPLC-QTOF-MS/MS-based metabolomic platform, we explored the changes of metabolic profiling in plasma/urine simultaneously between chronic kidney disease (CKD) induced from adenine excess and the protective effects of A. oxyphylla extract (AOE). The total twenty-one metabolites (twelve in urine and nine in plasma), up-regulated or down-regulated, were identified and contributed to CKD progress. Among these biomarkers, agmatine, CAMP, 7-methylguanine, hippuric acid, indoxyl sulfate, asparagines, kynurenic acid and p-cresol sulfate were restored back to the control-like level after the treatment of AOE (p
- Published
- 2015
29. Comparative pharmacokinetics of acteoside from total glycoside extracted from leaves ofRehmanniaand Dihuangye total glycoside capsule in normal and diabetic nephropathy rats
- Author
-
Dai, Xin-Xin, primary, Su, Shu-Lan, additional, Cai, Hong-Die, additional, Wei, Dan-Dan, additional, Zheng, Tian-Yao, additional, Zhu, Zhen-Hua, additional, Yan, Hui, additional, Shang, Er-Xin, additional, Guo, Sheng, additional, Qian, Da-Wei, additional, and Duan, Jin-Ao, additional
- Published
- 2017
- Full Text
- View/download PDF
30. Viral E6 is overexpressed via high viral load in invasive cervical cancer with episomal HPV16
- Author
-
Hong, Die, primary, Liu, Jia, additional, Hu, Ying, additional, Lu, Xiaonan, additional, Li, Baohua, additional, Li, Yang, additional, Hu, Dongxiao, additional, Lu, Weiguo, additional, Xie, Xing, additional, and Cheng, Xiaodong, additional
- Published
- 2017
- Full Text
- View/download PDF
31. Simultaneous Determination of Four Tanshinones by UPLC-TQ/MS and Their Pharmacokinetic Application after Administration of Single Ethanol Extract of Danshen Combined with Water Extract in Normal and Adenine-Induced Chronic Renal Failure Rats
- Author
-
Cai, Hong-Die, primary, Su, Shu-Lan, additional, Li, Yonghui, additional, Zhu, Zhenhua, additional, Guo, Jianming, additional, Zhu, Yue, additional, Guo, Sheng, additional, Qian, Dawei, additional, and Duan, Jinao, additional
- Published
- 2016
- Full Text
- View/download PDF
32. Differential systemic exposure to galangin after oral and intravenous administration to rats
- Author
-
Xiao Po Zhang, Hai Long Li, Wei Yong Lai, Feng Chen, Hong Die Cai, Jun Qing Zhang, Zhen Miao Qin, Yong Hui Li, Wei Wei Guan, Yin Feng Tan, and You Bin Li
- Subjects
Galangin ,Chemistry ,Metabolite ,Glucuronidation ,General Chemistry ,Pharmacology ,In vitro ,Bioavailability ,chemistry.chemical_compound ,Sulfation ,Administration routes ,Pharmacokinetics ,In vivo ,LC-MS/MS ,Research Article - Abstract
Background Galangin (3,5,7-trihydroxyflavone) is present in high concentrations in herbal medicine such as Alpinia officinarum Hance. Galangin shows multifaceted in vitro and in vivo biological activities. The number and position of hydroxyl groups in this molecule play an important role in these biological activities. However, these hydroxyl groups undergo glucuronidation and sulfation in in vitro assay system. However, the systemic exposure to galangin after dosing in animals and/or humans remains largely unknown. Thus it is not clear whether the galangin exists in the body at concentrations high enough for the biological effects. Furthermore, the metabolite identification and the corresponding plasma pharmacokinetics need to be characterized. Results Two LC-MS/MS methods were developed and validated and successfully applied to analyze the parent drug molecules and aglycones liberated from plasma samples via β-glucuronidase hydrolysis. Our major findings were as follows: (1) The routes of administration showed significant influences on the systemic exposure of galangin and its metabolites. (2) Galangin was preferentially glucuronidated after p.o. dosing but sulfated after i.v. medication. (3) Kaempferol conjugates were detected demonstrating that oxidation reaction occurred; however, both glucuronidation and sulfation were more efficient. (4) Oral bioavailability of free parent galangin was very low. Conclusions Systemic exposure to galangin and its metabolites was different in rat plasma between oral and intravenous administration. Further research is needed to characterize the structures of galangin conjugates and to evaluate the biological activities of these metabolites.
- Published
- 2015
33. Correction: Sha et al. Differences in Root Endophytic Bacterial Communities of Chinese Cork Oak (Quercus variabilis) Seedlings in Different Growth Years. Forests 2023, 14 , 1489.
- Author
-
Sha, Weilai, Hong, Die, Che, Yuying, Xue, Yafei, Kong, Yong, Yi, Xianfeng, Zhou, Jing, Yu, Guohong, and Liu, Baoxuan
- Subjects
CORK oak ,BACTERIAL communities ,OAK ,SEEDLINGS ,BACTERIAL DNA - Abstract
Reference 1 Sha W., Hong D., Che Y., Xue Y., Kong Y., Yi X., Zhou J., Yu G., Liu B. Differences in Root Endophytic Bacterial Communities of Chinese Cork Oak (Quercus variabilis) Seedlings in Different Growth Years. DNA Extraction and PCR Amplification i " has been made to "The root samples were cleaned using ultrasonic waves 3 times and disinfected with sodium hypochlorite 2 times for DNA extraction." Differences in Root Endophytic Bacterial Communities of Chinese Cork Oak (Quercus variabilis) Seedlings in Different Growth Years. [Extracted from the article]
- Published
- 2023
- Full Text
- View/download PDF
34. Comparative pharmacokinetics of acteoside from total glycoside extracted from leaves ofRehmanniaand Dihuangye total glycoside capsule in normal and diabetic nephropathy rats
- Author
-
Dandan Wei, Dawei Qian, Jin-Ao Duan, Erxin Shang, Sheng Guo, Hui Yan, Xinxin Dai, Zhenhua Zhu, Shulan Su, Hong-Die Cai, and Tianyao Zheng
- Subjects
Male ,0301 basic medicine ,medicine.medical_treatment ,Clinical Biochemistry ,Intraperitoneal injection ,030226 pharmacology & pharmacy ,Biochemistry ,Mass Spectrometry ,Analytical Chemistry ,Rats, Sprague-Dawley ,Diabetic nephropathy ,03 medical and health sciences ,0302 clinical medicine ,Drug Stability ,Glucosides ,Phenols ,Pharmacokinetics ,Drug Discovery ,medicine ,Animals ,Diabetic Nephropathies ,Glycosides ,Molecular Biology ,Chromatography, High Pressure Liquid ,Pharmacology ,chemistry.chemical_classification ,Chromatography ,biology ,Plant Extracts ,Chemistry ,Reproducibility of Results ,Glycoside ,Capsule ,General Medicine ,medicine.disease ,Streptozotocin ,Rehmannia glutinosa ,biology.organism_classification ,Rats ,Plant Leaves ,Rehmannia ,030104 developmental biology ,medicine.drug - Abstract
Rehmannia glutinosa Libosch (RG), is officially listed in the Chinese Pharmacopoeia and is widely used in China. In this paper, a sensitive and rapid ultra-performance liquid chromatography-mass spectrometry method including multiple-reaction monitoring mode was developed and applied to study the pharmacokinetic effect of acteoside from total glycoside extracted from the leaves of Rehmannia (TLR) and Dihuangye total glycoside capsule (DTG) in normal and diabetic nephropathy rats. The diabetic nephropathy rat model was induced by intraperitoneal injection of a small dose of streptozotocin and high-fat diet and plus 5% glucose drinking water. Samples of plasma of rats were obtained at different times after rats were administered TLR (7.2 g/kg) and DTG (360 mg/kg). After deproteinization by acetonitrile, the concentrations of acteoside in rats at different time points were detected by UPLC-TQ-MS method and pharmacokinetics parameters were calculated using DAS 3.2.8 software. A good linearity of acteoside was shown in the range of 8.51-3404.8 ng/m L (r2 = 0.9987). The mean extraction recovery of analyte was in the range of 63.55-79.49%, and the intra- and inter-day RSD values were
- Published
- 2017
35. Expression of E-, P- and N-Cadherin and Its Clinical Significance in Cervical Squamous Cell Carcinoma and Precancerous Lesions
- Author
-
Li, Baohua, primary, Shi, Haiyan, additional, Wang, Fenfen, additional, Hong, Die, additional, Lv, Weiguo, additional, Xie, Xing, additional, and Cheng, Xiaodong, additional
- Published
- 2016
- Full Text
- View/download PDF
36. Variants in human papillomavirus receptor and associated genes are associated with type-specific HPV infection and lesion progression of the cervix
- Author
-
Zou, Jian, primary, Cao, Zhu, additional, Zhang, Jianyang, additional, Chen, Tingting, additional, Yang, Shizhou, additional, Huang, Yongjie, additional, Hong, Die, additional, Li, Yang, additional, Chen, Xiaojing, additional, Wang, Xinyu, additional, Cheng, Xiaodong, additional, Lu, Weiguo, additional, and Xie, Xing, additional
- Published
- 2016
- Full Text
- View/download PDF
37. Comparative pharmacokinetics of acteoside from total glycoside extracted from leaves of Rehmannia and Dihuangye total glycoside capsule in normal and diabetic nephropathy rats.
- Author
-
Dai, Xin‐Xin, Su, Shu‐Lan, Cai, Hong‐Die, Wei, Dan‐Dan, Zheng, Tian‐Yao, Zhu, Zhen‐Hua, Yan, Hui, Shang, Er‐Xin, Guo, Sheng, Qian, Da‐Wei, and Duan, Jin‐Ao
- Abstract
Rehmannia glutinosa Libosch (RG), is officially listed in the Chinese Pharmacopoeia and is widely used in China. In this paper, a sensitive and rapid ultra-performance liquid chromatography-mass spectrometry method including multiple-reaction monitoring mode was developed and applied to study the pharmacokinetic effect of acteoside from total glycoside extracted from the leaves of Rehmannia (TLR) and Dihuangye total glycoside capsule (DTG) in normal and diabetic nephropathy rats. The diabetic nephropathy rat model was induced by intraperitoneal injection of a small dose of streptozotocin and high-fat diet and plus 5% glucose drinking water. Samples of plasma of rats were obtained at different times after rats were administered TLR (7.2 g/kg) and DTG (360 mg/kg). After deproteinization by acetonitrile, the concentrations of acteoside in rats at different time points were detected by UPLC-TQ-MS method and pharmacokinetics parameters were calculated using DAS 3.2.8 software. A good linearity of acteoside was shown in the range of 8.51-3404.8 ng/m L ( r
2 = 0.9987). The mean extraction recovery of analyte was in the range of 63.55-79.49%, and the intra- and inter-day RSD values were <8.8%. Compared with the normal group, the maximum plasma concentration, AUC0- t , AUC0-∞ and apparent plasma clearance corresponding dose in model group rats decreased significantly. After rats were administered TLR and DTG, the acteoside reached the maximum plasma concentration at about 15 min. The method proved to be simple, rapid and specific, and to be suitable for the determination of acteoside in plasma of diabetic nephropathy rats and pharmacokinetic study. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
38. Determination of α-hederin in rat plasma using liquid chromatography electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS) and its application to a pharmacokinetic study
- Author
-
Li, Hai-Long, primary, Qin, Zhen-Miao, additional, Cai, Hong-Die, additional, Tan, Yin-Feng, additional, Zhang, Xiao-Po, additional, Luo, Yu-Chao, additional, Li, Bei, additional, Chen, Feng, additional, and Zhang, Jun-Qing, additional
- Published
- 2015
- Full Text
- View/download PDF
39. Renal protective effect and action mechanism of Huangkui capsule and its main five flavonoids.
- Author
-
Cai, Hong-Die, Su, Shu-Lan, Qian, Da-Wei, Guo, Sheng, Tao, Wei-Wei, Cong, Xu Dong, Tang, Renmao, and Duan, Jin-Ao
- Subjects
- *
REACTIVE oxygen species , *ANIMAL experimentation , *BIOFLAVONOIDS , *CHRONIC kidney failure , *GENE expression , *CHINESE medicine , *RATS , *WESTERN immunoblotting ,THERAPEUTIC use of plant extracts - Abstract
Ethnopharmacological relevance The flower of Abelmoschus manihot (Linn.) Medicus ( A. manihot), as a traditional Chinese Herbal medicine, was used widely in China with efficacy of inducing diuresis for treating strangurtia, and subdhing swelling and detoxicating. It has been reported that Huangkui capsule, prepared by the extract of the flower of A. manihot , can reduce the content of urinary protein, serum creatinine and serum urea nitrogen in nephropathy rats and processes renoprotective activity, while the action mechanism need to illuminate deeply. Aims of the study In this study, we investigated the protection effect of Huangkui capsule on tubulointerstitial fibrosis in chronic renal failure (CRF) rats and its mechanism against high glucose-induced epithelial to mesenchymal transition (EMT) in renal tubular epithelial cells (HK-2) of its bioactive components. Materials and methods The animals were divided into normal group, CRF model group and Huangkui capsule-treated group. Hematoxylin eosin (HE) staining and Masson staining were applied to observe pathological changes in renal tissue of different groups. Biochemical indicators including serum urea nitrogen (BUN), urine protein (UP) and serum creatinine (Scr) were measured according to the manufacturer's instructions of kits. HK-2 cell damaged model was established to access the protection effect and action mechanism of five main flavonoids from Huangkui capsule. The experimental cells were divided into eight groups: control group, model group, positive drug group and five main flavonoids treated groups. The dichlorodihydrofluorescein diacetate (DCFH-DA) assay was used to determine the reactive oxygen species (ROS) in different groups. Western blot was applied to analyze the expression of pathogenesis-related proteins in different groups. Results The results stated that Huangkui capsule significantly inhibited the elevation of Scr, BUN, UP, the expression of α-smooth muscle actin (α-SMA), phosphorylation-extracellular signal-regulated kinase (p-ERK1/2), NADPH Oxidase 1, NADPH Oxidase 2 and NADPH Oxidase 4 in adenine-induced CRF rats. The main bioactive components of quercetin (QT), hyperoside (HY), isoquercitrin (IQT), gossypetin-8- O - β -D-glucuronide (GG) and quercetin-3′- O -glucoside (QG) at the dosage of 100 µM, like NADPH oxidase inhibitor diphenyleneiodonium, exhibited a significant effect on inhibiting the expression of α-SMA, p-ERK1/2, NADPH Oxidase 1, NADPH Oxidase 2 and NADPH Oxidase 4 in high glucose-induced HK-2 cells, especially GG. Conclusions These results demonstrated that Huangkui capsule and the flavonoids components prevent tubulointerstitial fibrosis in CRF rat involvement in the action mechanism of inhibiting NADPH oxidase/ROS/ERK pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
40. Viral E6 is overexpressed via high viral load in invasive cervical cancer with episomal HPV16.
- Author
-
Die Hong, Jia Liu, Ying Hu, Xiaonan Lu, Baohua Li, Yang Li, Dongxiao Hu, Weiguo Lu, Xing Xie, Xiaodong Cheng, Hong, Die, Liu, Jia, Hu, Ying, Lu, Xiaonan, Li, Baohua, Li, Yang, Hu, Dongxiao, Lu, Weiguo, Xie, Xing, and Cheng, Xiaodong
- Subjects
GENETIC overexpression ,VIRAL load ,CERVICAL cancer treatment ,EPISOMES ,PAPILLOMAVIRUSES ,GENOMES ,CARCINOGENESIS ,PREVENTION ,CANCER invasiveness ,GENES ,PAPILLOMAVIRUS diseases ,POLYMERASE chain reaction ,PROTEINS ,CERVIX uteri tumors ,VIRAL physiology ,DNA methylation ,CERVICAL intraepithelial neoplasia - Abstract
Background: The integration of HR-HPV genome into host DNA is regarded as a key step for the development of cervical cancer. However, HR-HPV genome indeed exists as episome except for integrant. It may be alternative mechanisms in episome-associated carcinogenesis, although, by which HPV 16 episome induces cervical carcinogenesis is unclear now.Methods: Ninety-three invasive cervical cancer tissues with HPV16 positive were collected. Viral physical status was calculated from comparing E2 to E6-copies and detection of viral load was made with realtime-PCR using copy numbers of E6. HPV16 E6 mRNA transcript levels were measured by realtime-PCR. The methylation frequency of HPV16 promoter was detected by PCR and pyrosequencing.Results: In 93 samples, 21.5% (20/93) presented purely integrated viral genome, 53.8% (50/93) mixed viral genome, and 24.7% (23/93) purely episomal viral genome. Mean E6 expression in samples with purely episomal viral genomes was 7.13-fold higher than that with purely integrated viral genomes. Meanwhile, viral load in samples with purely episomal viral genomes was 4.53-fold higher than that with purely integrated viral genomes. E6 mRNA expression increased with the viral load in purely episomal cases. There were no differences of mean methylation frequency between purely episomal and integrated virus and among five CpG positions of HPV16 promoter for all samples. And there also was no correlation between E6 mRNA expression and methylation of HPV16 promoter among all samples with purely HPV16 episomal virus.Conclusions: HPV16 with the purely episomal viral genomes exists in a definite proportion of invasive cervical cancer, and episomal HPV16 also overexpresses E6 mRNA, probably through a high level of viral load. [ABSTRACT FROM AUTHOR]- Published
- 2017
- Full Text
- View/download PDF
41. Simultaneous Determination of Four Tanshinones by UPLC-TQ/MS and Their Pharmacokinetic Application after Administration of Single Ethanol Extract of Danshen Combined withWater Extract in Normal and Adenine-Induced Chronic Renal Failure Rats.
- Author
-
Hong-Die Cai, Shu-Lan Su, Yonghui Li, Zhenhua Zhu, Jianming Guo, Yue Zhu, Sheng Guo, Dawei Qian, and Jinao Duan
- Subjects
PHARMACOKINETICS ,ETHANOL ,SALVIA miltiorrhiza ,PLANT extracts ,CHRONIC kidney failure ,LABORATORY rats - Abstract
Salvia miltiorrhiza, one of the major traditional Chinese medicines, is commonly used and the main active ingredients--tanshinones--possess the ability to improve renal function. In this paper, the UPLC-TQ/MS method of simultaneously determining four tanshinones--tanshinone IIA, dihydrotanshinone I, tanshinone I, and cryptotanshinone--was established and applied to assess the pharmacokinetics in normal and chronic renal failure (CRF) rat plasma. The pharmacokinetics of tanshinones in rats were studied after separately intragastric administration of Salvia miltiorrhiza ethanol extract (SMEE) (0.65 g/kg), SMEE (0.65 g/kg) combined with Salvia miltiorrhiza water extract (SMWE) (1.55 g/kg). The results showed Cmax and AUC
0-t of tanshinone IIA, tanshinone I, cryptotanshinone reduced by 50%~80% and CLz/F increased by 2~4 times (p < 0.05) in model group after administrated with SMEE. Nevertheless, after intragastric administration of a combination of SMWE and SMEE, the Cmax and AUC0-t of four tanshinones were upregulated and CLz/F was downregulated, which undulated similarity from the model group to the normal group with compatibility of SMEE and SMWE. These results hinted that SMWE could improve the bioavailability of tanshinones in CRF rats, which provides scientific information for further exploration the mechanism of the combination of SMWE and SMEE and offers a reference for clinical administration of Salvia miltiorrhiza. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
42. Differential systemic exposure to galangin after oral and intravenous administration to rats.
- Author
-
Feng Chen, Yin-Feng Tan, Hai-Long Li, Zhen-Miao Qin, Hong-Die Cai, Wei-Yong Lai, Xiao-Po Zhang, Yong-Hui Li, Wei-Wei Guan, You-Bin Li, and Jun-Qing Zhang
- Subjects
GINKGO -- Health aspects ,HERBAL medicine ,BIOCHEMISTRY ,METABOLOMICS ,DEHYDRATION reactions - Abstract
Background: Galangin (3,5,7-trihydroxyflavone) is present in high concentrations in herbal medicine such as Alpinia officinarum Hance. Galangin shows multifaceted in vitro and in vivo biological activities. The number and position of hydroxyl groups in this molecule play an important role in these biological activities. However, these hydroxyl groups undergo glucuronidation and sulfation in in vitro assay system. However, the systemic exposure to galangin after dosing in animals and/or humans remains largely unknown. Thus it is not clear whether the galangin exists in the body at concentrations high enough for the biological effects. Furthermore, the metabolite identification and the corresponding plasma pharmacokinetics need to be characterized. Results: Two LC-MS/MS methods were developed and validated and successfully applied to analyze the parent drug molecules and aglycones liberated from plasma samples via β-glucuronidase hydrolysis. Our major findings were as follows: (1) The routes of administration showed significant influences on the systemic exposure of galangin and its metabolites. (2) Galangin was preferentially glucuronidated after p.o. dosing but sulfated after i.v. medication. (3) Kaempferol conjugates were detected demonstrating that oxidation reaction occurred; however, both glucuronidation and sulfation were more efficient. (4) Oral bioavailability of free parent galangin was very low. Conclusions: Systemic exposure to galangin and its metabolites was different in rat plasma between oral and intravenous administration. Further research is needed to characterize the structures of galangin conjugates and to evaluate the biological activities of these metabolites. [ABSTRACT FROM AUTHOR]
- Published
- 2015
- Full Text
- View/download PDF
43. A sensitive and cost-effective LC-ESI-MS/MS method for quantitation of euscaphic acid in rat plasma using optimized formic acid concentration in the mobile phase.
- Author
-
Chen, Feng, Li, Hai-Long, Tan, Yin-Feng, Lai, Wei-Yong, Qin, Zhen-Miao, Cai, Hong-Die, Li, Yong-Hui, Zhang, Jun-Qing, and Zhang, Xiao-Po
- Published
- 2014
- Full Text
- View/download PDF
44. [Effect of Salviae Miltiorrhizae Radix et Rhizoma on diversity of intestinal flora in diabetic nephropathy rats].
- Author
-
Cai HD, Su SL, Guo JM, and Duan JA
- Subjects
- Animals, Rats, Diabetes Mellitus, Experimental drug therapy, Diabetic Nephropathies drug therapy, Drugs, Chinese Herbal, Gastrointestinal Microbiome, Salvia miltiorrhiza
- Abstract
This study aimed to explore the effect of Salviae Miltiorrhizae Radix et Rhizoma, its stems and leaves on the diversity of intestinal microflora in rats with diabetic kidney injury. Diabetic rats model was established by feeding high glucose and high fat diet and 5% glucose solution with intraperitoneal injection of 30 mg·kg~(-1) streptozocin(STZ). The rats were randomly divided into normal group, model group, irbesartan control group, Huangkui Capsules control group, as well as low, middle and high dose groups of Sal-viae Miltiorrhizae Radix et Rhizoma, its stems and leaves. After administration for 2 weeks, 16 S rRNA technique was used to analyze the diversity of intestinal microflora in the feces of each group. The results showed rats in the model group developed renal tubular epithelial vacuole degeneration and a large amount of inflammatory cell infiltration in the renal interstitium. A small amount of inflammatory cell infiltration was seen in each administration group. The kidney structure of rats in irbesartan group, Huangkui Capsules group, high-dose group of Salviae Miltiorrhizae Radix et Rhizoma and its stem water extract, as well as high dose group of Salviae Miltiorrhizae Radix et Rhizoma and its stem ethnol extract group was close to the normal group. The diversity and structure of intestinal flora in the model group were significantly different from those in the normal group. Each administration group improved the fecal flora diversity in rats with diabetic kidney injury to a certain extent, especially the high dose of Salviae Miltiorrhizae Radix et Rhizoma and its stems water extract. Different flora were found in feces of diabetic nephropathy model rats on class, order, family and genus levels. On families and genera levels, the relative abundance of Bifidobacterium, Turicibacter, Peptostreptococcaceae, Desulfovibrio, and SMB53 showed an upward trend in model group, but that of Lactobacillus, Clostridium, Rikenella, Rumen fungi showed a downward trend. The administration groups can improve the relative abundance of the above intestinal flora in the model rats to a normal-like level. The results of this study provide a reference for resource utilization and further development of Salviae Miltiorrhizae Radix et Rhizoma.
- Published
- 2021
- Full Text
- View/download PDF
45. [Diagnosis and Treatment of Primary Aldosteronism in West China Hospital of Sichuan University from 2009 to 2018].
- Author
-
Liu HD, Li YM, Wang W, Li QR, Chen T, Zhu YC, Ren Y, and Tian HM
- Subjects
- Aldosterone, China epidemiology, Female, Humans, Male, Universities, Adrenal Gland Neoplasms, Hyperaldosteronism diagnosis, Hyperaldosteronism epidemiology, Hyperaldosteronism therapy, Hypertension diagnosis, Hypertension epidemiology
- Abstract
Objective: To summary the clinical diagnosis and treatment of primary aldosteronism (PA) in West China Hospital (WCH) of Sichuan University during 2009-2018., Methods: This study enrolled the patients diagnosed as PA and admitted in WCH of Sichuan University from January 2009 to December 2018. The information of the patients including epidemiological and clinical data, diagnosis and treatment as well as therapeutic outcomes were collected and analyzed., Results: A total of 853 patients with 1 248 diagnostic cases were included in the analysis, and the diagnosis cases of PA increased year by year from 2009 to 2018. Most patients (74.33%) were confirmed the diagnosis in the Department of Endocrinology and Metabolism and then admitted to the hospital. PA was more frequent in female than in male, with a ratio of female to male about 1.34∶1. Hypertension was the most common chief complaint, in contrast, the proportion of fatigue and/or numbness as the symptoms of hypokalemia was declining. More and more patients were diagnosed because of imaging examination founding adrenal incidentoma. After 2016, more and more patients were diagnosed by recumbent saline suppression test and captopril challenge test, and the number of adrenal venous sampling to classify PA subtypes was increasing to help choosing different treatment options. The proportion of surgical treatment decreased year by year, and more and more patients adopted medical treatment or transferred to surgery with combined treatment instead of simple operation., Conclusion: During the past 10 years, remarkable progress was made in the diagnosis and treatment of PA. Hypertension was the most important clinical manifestation of PA, so the screening of PA in hypertensive patients should be strengthened. Adrenal incidentaloma has become prevalent manifestation of PA with an increasing trend, which needs more attention in clinical practice., (Copyright© by Editorial Board of Journal of Sichuan University (Medical Science Edition).)
- Published
- 2020
- Full Text
- View/download PDF
46. [Silkworm excrement bacterial communities diversity in different instars based on 16S rDNA sequence analysis].
- Author
-
Zheng TY, Su SL, Cai HD, Dai XX, Ouyang Z, and Duan JA
- Subjects
- Animals, Biodiversity, DNA, Bacterial genetics, High-Throughput Nucleotide Sequencing, Phylogeny, RNA, Ribosomal, 16S genetics, Sequence Analysis, DNA, Bacteria classification, Bombyx microbiology, Feces microbiology
- Abstract
This paper investigated the diversity of the silkworm excrement bacterial communities in different ages before and after drying, aiming to clarify the differences of bacterial communities in composition and bacterial abundance and the influences of drying treatment, and provide scientific basis for the efficacy of scientific connotation and utilization of silkworm excrement. High-throughput sequencing technique was used to measure the sequence of 16S rDNA-V4 variable region of bacteria in silkworm excrement. QIIME, Mothur and PICRUSt software programs were employed to sort and calculate the number of sequences and operational taxonomic units (OTUs) for each sample. Thereafter, the abundance, distribution, alpha diversity index of species, beta diversity and bacterial communities diversity among different sample groups and predicted the bacterial gene functions were analyzed. In this study, the numbers of effective sequences for six samples were 259 250; the rarefaction curves showed a sufficient sequencing depth, and the number of OTUs was close to saturation. The bacteria in silkworm excrement belonged to the following five phylums: Proteobacteria (89.3%), Actinobacteria (5.0%), Firmicutes (4.4%), Bacteroidetes (1.1%) and Cyanobacteria (0.2%). The dominant specie was Cyanobacteria of the total bacteria identified, respectively. The abundances and diversities of the silkworm excrement bacterial communities have been reduced after drying treatment, especially the silkworm excrement of the fifth instar. PICRUSt analysis was performed to show that abundance of the functional genes such as membrane transport, carbohydrate metabolism, amino acid metabolism, lipid metabolism, nucleotide metabolism, cellular processes and signaling were relatively high. The result showed that the drying treatment could decreased the species and numbers of pathogenic bacteria in silkworm excrement obviously and improve the quality of medicinal materials. Compared with the lower ages, silkworm excrement of fifth instar seems like to be more suitable for use in medicine. Illumina MiSeq high-throughput sequencing system provides a more accurate and scientific data resource for the study of bacteria in silkworm excrement., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2017
- Full Text
- View/download PDF
47. [Antidepressant activity of flavonoid ethanol extract of Abelmoschus manihot corolla with BDNF up-regulation in the hippocampus].
- Author
-
Cai HD, Tao WW, Su SL, Guo S, Zhu Y, Guo JM, Qian DW, Cong XD, Tang RM, and Duan JA
- Subjects
- Animals, China, Depressive Disorder, Major, Drugs, Chinese Herbal pharmacology, Ethanol, Hindlimb Suspension, Hippocampus metabolism, Mice, Quercetin analogs & derivatives, Swimming, Up-Regulation, Abelmoschus chemistry, Antidepressive Agents pharmacology, Brain-Derived Neurotrophic Factor metabolism, Flavonoids pharmacology, Hippocampus drug effects, Plant Extracts pharmacology
- Abstract
Abelmoschus manihot (L.) Medic., a folk herbal medicine in China, is a flowering plant belonging to Abelmoschus L. genus and Malvaceae family, which has been reported with an antidepressant activity. The study was designed to isolate flavonoids from Abelmoschus manihot corolla and explore the action mechanism of antidepressant activities. The flavonoids were isolated and purified by D101 macroporous resin column, polyamide column and Sephadex LH-20 sequentially and identified as myricetin-3-O-β-D-glucoside (1), gossypetin-8-O-β-D-glucuronide (2, G-8-G), gossypetin-3'-O-β-D-glucoside (3), quercetin-3'-glucoside (4, Q-3-G), isoquercitrin (5, IQT), hyperoside (6, HY), myricetin (7), quercetin (8, QT). Compounds 2, 4, 5, 6 and 8 (15, 30 and 60 mg·kg−1) were orally administered to mice and the reaction was observed in tail suspension test (TST) and forced swimming test (FST). Western blot analysis was used in determination of the protein expressions of brain-derived neurotrophic factor (BDNF), tyrosine receptor kinase B (TrkB) and phosphorylation eukaryotic elongation factor 2 (p-eEF2). The results revealed that only Q-3-G and G-8-G (15, 30, 60 mg ·kg−1) significantly reduced the immobility time in FST and TST. Furthermore, Q-3-G and G-8-G remarkably increased the expression of BDNF and TrkB, and decreased the expression of p-eEF2. These results suggest that Q-3-G and G-8-G had an obvious antidepressant activity via up-regulation of BDNF expression. The new observation will provide a new direction in the development of antidepressant in the treatment of major depressive disorder (MDD).
- Published
- 2017
48. [Effect of flavonoids from Abelmoschus manihot on proliferation, differentiation of 3T3-L1 preadipocyte and insulin resistance].
- Author
-
Cai HD, Su SL, Guo S, Zhu Y, Qian DW, Tao WW, and Duan JA
- Subjects
- 3T3-L1 Cells, Animals, Mice, Abelmoschus chemistry, Adipocytes drug effects, Cell Differentiation, Cell Proliferation, Flavonoids pharmacology, Insulin Resistance
- Abstract
Abelmoschus manihot was rich in flavonoids, which has been reported the activity on protecting angiocarpy and improving renal function. This study aimed to explore the action mechanism of five flavonoids from A. manihot on how to ameliorating insulin resistance through the regulation of the glucose and expression of PPARγ, C/EBPα, SREBP-1, resistin, visfatin, adiponectin in 3T3-L1 adipocytes. After the 3T3-L1 preadipocytes were differentiated into mature adipocytes, insulin resistance model was built. Insulin resistance adipocytes were treated with 5, 100 μmol•L⁻¹ quercetin, isoquercitrin, hyperoside, quercitrin-3'-O-glucoside, gossypetin-8-O-β-glucoside. The glucose was indirectly determined by BCA kit. The mRNA expression levels of PPARγ, C/EBPα, SREBP-1, resistin, visfatin, adiponectin were detected by real-time quantitative PCR. Results showed that five flavonoids at 5 μmol•L⁻¹ could accelerate preadipocytes proliferation and inhibit that at 100 μmol•L⁻¹ Compared with the normal group, glucose uptake reduced significantly in model group (P<0.01). With the treatment of five flavonoids at 100 μmol•L⁻¹, glucose consumption increased significantly (P<0.01). The high expression of PPARγ, C/EBPα, adiponectin expression was significantly increased (P<0.01), and low expression of SREBP-1, resistin, visfatin after respective administration with five flavonoids at 100 μmol•L-1 promoted adipocyte differentiation. This study showed that, HY, JY, QT, QG, GG can control preadipocytes proliferation, promote adipocyte differentiation and regulate the expression of relative factors with lipid metabolism, such as PPARγ, C/EBPα, SREBP-1, adiponectin, resistin, visfatin, increasing glucose utilization and improving insulin resistance in 3T3-L1 adipocyte., Competing Interests: The authors of this article and the planning committee members and staff have no relevant financial relationships with commercial interests to disclose., (Copyright© by the Chinese Pharmaceutical Association.)
- Published
- 2016
- Full Text
- View/download PDF
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.