1. Phenotyping of autoreactive B cells with labeled nucleosomes in 56R transgenic mice
- Author
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Pauline Soulas-Sprauel, Delphine Bouis, Mickaël Martin, Anne Sophie Korganow, Thierry Martin, Jean Louis Pasquali, Vincent Gies, Immuno-Rhumatologie Moléculaire, Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), Immunopathologie et chimie thérapeutique (ICT), Centre National de la Recherche Scientifique (CNRS)-Institut de biologie moléculaire et cellulaire (IBMC), and Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0301 basic medicine ,Transgene ,lcsh:Medicine ,Autoimmunity ,Mice, Transgenic ,[SDV.BC]Life Sciences [q-bio]/Cellular Biology ,medicine.disease_cause ,Article ,Flow cytometry ,Immunophenotyping ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Sciences du Vivant [q-bio]/Immunologie ,lcsh:Science ,B cell ,B-Lymphocytes ,Multidisciplinary ,biology ,medicine.diagnostic_test ,lcsh:R ,Autoantibody ,Germinal center ,Flow Cytometry ,3. Good health ,Cell biology ,Nucleosomes ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,Phenotype ,Antibodies, Antinuclear ,Immunology ,biology.protein ,lcsh:Q ,Antibody ,Spleen ,030215 immunology - Abstract
The phenotypic characterization of self-reactive B cells producing autoantibodies is one of the challenges to get further insight in the physiopathology of autoimmune diseases. We took advantage of our previously developed flow cytometry method, using labeled nucleosomes, prominent autoantigens in systemic lupus erythematosus, to analyze the phenotype of self-reactive B cells in the anti-DNA B6.56R mouse model. We showed that splenic anti-nucleosome B cells express mostly kappa light chains and harbor a marginal zone phenotype. Moreover, these autoreactive B cells fail to acquire a germinal center phenotype and are less abundant in the transitional T3 compartment. In conclusion, the direct detection of autoreactive B cells helped determine their phenotypic characteristics and provided a more direct insight into the B cell tolerance process in B6.56R mice. This method constitutes an interesting new tool to study the mechanisms of B cell tolerance breakdown in B6.56R mice crossed with autoimmune prone models.
- Published
- 2017