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31 results on '"Russell, Ronnie M."'

1. CD4 receptor diversity represents an ancient protection mechanism against primate lentiviruses

Author

Russell, Ronnie M., Bibollet-Ruche, Frederic, Liu, Weimin, Sherrill-Mix, Scott, Li, Yingying, Connell, Jesse, Loy, Dorothy E., Trimboli, Stephanie, Smith, Andrew G., Avitto, Alexa N., Gondim, Marcos V. P., Plenderleith, Lindsey J., Wetzel, Katherine S., Collman, Ronald G., Ayouba, Ahidjo, Esteban, Amandine, Peeters, Martine, Kohler, William J., Miller, Richard A., François-Souquiere, Sandrine, Switzer, William M., Hirsch, Vanessa M., Marx, Preston A., Piel, Alex K., Stewart, Fiona A., Georgiev, Alexander V., Sommer, Volker, Bertolani, Paco, Hart, John A., Hart, Terese B., Shaw, George M., Sharp, Paul M., and Hahn, Beatrice H.

Published
2021

2. Predictors of Nonseroconversion after SARS-CoV-2 Infection

Author

Liu, Weimin, Russell, Ronnie M., Bibollet-Ruche, Frederic, Skelly, Ashwin N., Sherrill-Mix, Scott, Freeman, Drew A., Stoltz, Regina, Lindemuth, Emily, Lee, Fang-Hua, Sterrett, Sarah, Bar, Katharine J., Erdmann, Nathaniel, Gouma, Sigrid, Hensley, Scott E., Ketas, Thomas, Cupo, Albert, Portillo, Victor M. Cruz, Moore, John P., Bieniasz, Paul D., Hatziioannou, Theodora, Massey, Greer, Minyard, Mary-Beth, Saag, Michael S., Davis, Randall S., Shaw, George M., Britt, William J., Leal, Sixto M., Jr., Goepfert, Paul, and Hahn, Beatrice H.

Subjects
Antigen-antibody reactions -- Research, Immunological research, Health
Abstract

Coronavirus disease (COVID-19) is typically diagnosed by reverse transcription PCR (RT-PCR) amplification of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA from nasopharyngeal fluids (1). RT-PCR yields cycle threshold ([C.sub.t]) [...]

Published
2021
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5. Single-cell transcriptomics identifies prothymosin α restriction of HIV-1 in vivo

Author

Geretz, Aviva, primary, Ehrenberg, Philip K., additional, Clifford, Robert J., additional, Laliberté, Alexandre, additional, Prelli Bozzo, Caterina, additional, Eiser, Daina, additional, Kundu, Gautam, additional, Yum, Lauren K., additional, Apps, Richard, additional, Creegan, Matthew, additional, Gunady, Mohamed, additional, Shangguan, Shida, additional, Sanders-Buell, Eric, additional, Sacdalan, Carlo, additional, Phanuphak, Nittaya, additional, Tovanabutra, Sodsai, additional, Russell, Ronnie M., additional, Bibollet-Ruche, Frederic, additional, Robb, Merlin L., additional, Michael, Nelson L., additional, Ake, Julie A., additional, Vasan, Sandhya, additional, Hsu, Denise C., additional, Hahn, Beatrice H., additional, Kirchhoff, Frank, additional, and Thomas, Rasmi, additional

Published
2023
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6. CD4 receptor diversity in chimpanzees protects against SIV infection

Author

Bibollet-Ruche, Frederic, Russell, Ronnie M., Liu, Weimin, Stewart-Jones, Guillaume B. E., Sherrill-Mix, Scott, Li, Yingying, Learn, Gerald H., Smith, Andrew G., Gondim, Marcos V. P., Plenderleith, Lindsey J., Decker, Julie M., Easlick, Juliet L., Wetzel, Katherine S., Collman, Ronald G., Ding, Shilei, Finzi, Andrés, Ayouba, Ahidjo, Peeters, Martine, Leendertz, Fabian H., van Schijndel, Joost, Goedmakers, Annemarie, Ton, Els, Boesch, Christophe, Kuehl, Hjalmar, Arandjelovic, Mimi, Dieguez, Paula, Murai, Mizuki, Colin, Christelle, Koops, Kathelijne, Speede, Sheri, Gonder, Mary K., Muller, Martin N., Sanz, Crickette M., Morgan, David B., Atencia, Rebecca, Cox, Debby, Piel, Alex K., Stewart, Fiona A., Ndjango, Jean-Bosco N., Mjungu, Deus, Lonsdorf, Elizabeth V., Pusey, Anne E., Kwong, Peter D., Sharp, Paul M., Shaw, George M., and Hahn, Beatrice H.

Published
2019

7. Identification of novel Mycobacterium tuberculosis CD4 T-cell antigens via high throughput proteome screening

Author

Nayak, Kaustuv, Jing, Lichen, Russell, Ronnie M, Davies, D Huw, Hermanson, Gary, Molina, Douglas M, Liang, Xiaowu, Sherman, David R, Kwok, William W, Yang, Junbao, Kenneth, John, Ahamed, Syed F, Chandele, Anmol, Murali-Krishna, Kaja, and Koelle, David M

Subjects
Medical Microbiology, Biomedical and Clinical Sciences, Immunology, HIV/AIDS, Immunization, Clinical Research, Orphan Drug, Biotechnology, Prevention, Biodefense, Vaccine Related, Infectious Diseases, Tuberculosis, Rare Diseases, Infection, Good Health and Well Being, Adult, Antigens, Bacterial, Bacterial Proteins, CD4-Positive T-Lymphocytes, Cell Proliferation, Cell Separation, Cells, Cultured, Cross Reactions, Cytokines, Epitopes, T-Lymphocyte, High-Throughput Screening Assays, Humans, Latent Tuberculosis, Lymphocyte Activation, Middle Aged, Mycobacterium tuberculosis, Open Reading Frames, Proteomics, Tuberculosis Vaccines, CD4, T-cell, Antigen, CD137, Malate synthase, Tetramer, Medical and Health Sciences, Microbiology, Clinical sciences, Medical microbiology
Abstract

Elicitation of CD4 IFN-gamma T cell responses to Mycobacterium tuberculosis (MTB) is a rational vaccine strategy to prevent clinical tuberculosis. Diagnosis of MTB infection is based on T-cell immune memory to MTB antigens. The MTB proteome contains over four thousand open reading frames (ORFs). We conducted a pilot antigen identification study using 164 MTB proteins and MTB-specific T-cells expanded in vitro from 12 persons with latent MTB infection. Enrichment of MTB-reactive T-cells from PBMC used cell sorting or an alternate system compatible with limited resources. MTB proteins were used as single antigens or combinatorial matrices in proliferation and cytokine secretion readouts. Overall, our study found that 44 MTB proteins were antigenic, including 27 not previously characterized as CD4 T-cell antigens. Antigen truncation, peptide, NTM homology, and HLA class II tetramer studies confirmed malate synthase G (encoded by gene Rv1837) as a CD4 T-cell antigen. This simple, scalable system has potential utility for the identification of candidate MTB vaccine and biomarker antigens.

Published
2015

8. Resistance to type 1 interferons is a major determinant of HIV-1 transmission fitness

Author

Iyer, Shilpa S., Bibollet-Ruche, Frederic, Sherrill-Mix, Scott, Learn, Gerald H., Plenderleith, Lindsey, Smith, Andrew G., Barbian, Hannah J., Russell, Ronnie M., Gondim, Marcos V. P., Bahari, Catherine Y., Shaw, Christiana M., Li, Yingying, Decker, Timothy, Haynes, Barton F., Shaw, George M., Sharp, Paul M., Borrow, Persephone, and Hahn, Beatrice H.

Published
2017

9. A Germline-Targeting Chimpanzee SIV Envelope Glycoprotein Elicits a New Class of V2-Apex Directed Cross-Neutralizing Antibodies

Author

Bibollet-Ruche, Frederic, primary, Russell, Ronnie M., additional, Ding, Wenge, additional, Liu, Weimin, additional, Li, Yingying, additional, Wagh, Kshitij, additional, Wrapp, Daniel, additional, Habib, Rumi, additional, Skelly, Ashwin N., additional, Roark, Ryan S., additional, Sherrill-Mix, Scott, additional, Wang, Shuyi, additional, Rando, Juliette, additional, Lindemuth, Emily, additional, Cruickshank, Kendra, additional, Park, Younghoon, additional, Baum, Rachel, additional, Carey, John W., additional, Connell, Andrew Jesse, additional, Li, Hui, additional, Giorgi, Elena E., additional, Song, Ge S., additional, Ding, Shilei, additional, Finzi, Andrés, additional, Newman, Amanda, additional, Hernandez, Giovanna E., additional, Machiele, Emily, additional, Cain, Derek W., additional, Mansouri, Katayoun, additional, Lewis, Mark G., additional, Montefiori, David C., additional, Wiehe, Kevin J., additional, Alam, S. Munir, additional, Teng, I-Ting, additional, Kwong, Peter D., additional, Andrabi, Raiees, additional, Verkoczy, Laurent, additional, Burton, Dennis R., additional, Korber, Bette T., additional, Saunders, Kevin O., additional, Haynes, Barton F., additional, Edwards, Robert J., additional, Shaw, George M., additional, and Hahn, Beatrice H., additional

Published
2023
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11. A germline-targeting chimpanzee SIV envelope glycoprotein elicits a new class of V2-apex directed cross-neutralizing antibodies

Author

Bibollet-Ruche, Frederic, primary, Russell, Ronnie M., additional, Ding, Wenge, additional, Liu, Weimin, additional, Li, Yingying, additional, Wagh, Kshitij, additional, Wrapp, Daniel, additional, Habib, Rumi, additional, Skelly, Ashwin N., additional, Roark, Ryan S., additional, Sherrill-Mix, Scott, additional, Wang, Shuyi, additional, Rando, Juliette, additional, Lindemuth, Emily, additional, Cruickshank, Kendra, additional, Park, Younghoon, additional, Baum, Rachel, additional, Connell, Andrew Jesse, additional, Li, Hui, additional, Giorgi, Elena E., additional, Song, Ge S., additional, Ding, Shilei, additional, Finzi, Andrés, additional, Newman, Amanda, additional, Hernandez, Giovanna E., additional, Machiele, Emily, additional, Cain, Derek W., additional, Mansouri, Katayoun, additional, Lewis, Mark G., additional, Montefiori, David C., additional, Wiehe, Kevin J., additional, Alam, S. Munir, additional, Teng, I-Ting, additional, Kwong, Peter D., additional, Andrabi, Raiees, additional, Verkoczy, Laurent, additional, Burton, Dennis R., additional, Korber, Bette T., additional, Saunders, Kevin O., additional, Haynes, Barton F., additional, Edwards, Robert J., additional, Shaw, George M., additional, and Hahn, Beatrice H., additional

Published
2022
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13. Convalescent plasma-mediated resolution of COVID-19 in a patient with humoral immunodeficiency

Author

Honjo, Kazuhito, Russell, Ronnie M., Li, Ran, Liu, Weimin, Stoltz, Regina, Tabengwa, Edlue M., Hua, Yutao, Prichard, Lynn, Kornbrust, Ashton N., Sterrett, Sarah, Marques, Marisa B., Lima, Jose L., Lough, Chris M., McCarty, Todd P., Ketas, Thomas J., Hatziioannou, Theodora, Bieniasz, Paul D., Redden, David T., Moore, John P., Goepfert, Paul A., Heath, Sonya L., Hahn, Beatrice H., and Davis, Randall S.

Published
2021
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14. Heightened resistance to host type 1 interferons characterizes HIV-1 at transmission and after antiretroviral therapy interruption

Author

Gondim, Marcos V. P., primary, Sherrill-Mix, Scott, additional, Bibollet-Ruche, Frederic, additional, Russell, Ronnie M., additional, Trimboli, Stephanie, additional, Smith, Andrew G., additional, Li, Yingying, additional, Liu, Weimin, additional, Avitto, Alexa N., additional, DeVoto, Julia C., additional, Connell, Jesse, additional, Fenton-May, Angharad E., additional, Pellegrino, Pierre, additional, Williams, Ian, additional, Papasavvas, Emmanouil, additional, Lorenzi, Julio C. C., additional, Salantes, D. Brenda, additional, Mampe, Felicity, additional, Monroy, M. Alexandra, additional, Cohen, Yehuda Z., additional, Heath, Sonya, additional, Saag, Michael S., additional, Montaner, Luis J., additional, Collman, Ronald G., additional, Siliciano, Janet M., additional, Siliciano, Robert F., additional, Plenderleith, Lindsey J., additional, Sharp, Paul M., additional, Caskey, Marina, additional, Nussenzweig, Michel C., additional, Shaw, George M., additional, Borrow, Persephone, additional, Bar, Katharine J., additional, and Hahn, Beatrice H., additional

Published
2021
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15. Recapitulation of HIV-1 Env-antibody coevolution in macaques leading to neutralization breadth

Author

Roark, Ryan S., primary, Li, Hui, additional, Williams, Wilton B., additional, Chug, Hema, additional, Mason, Rosemarie D., additional, Gorman, Jason, additional, Wang, Shuyi, additional, Lee, Fang-Hua, additional, Rando, Juliette, additional, Bonsignori, Mattia, additional, Hwang, Kwan-Ki, additional, Saunders, Kevin O., additional, Wiehe, Kevin, additional, Moody, M. Anthony, additional, Hraber, Peter T., additional, Wagh, Kshitij, additional, Giorgi, Elena E., additional, Russell, Ronnie M., additional, Bibollet-Ruche, Frederic, additional, Liu, Weimin, additional, Connell, Jesse, additional, Smith, Andrew G., additional, DeVoto, Julia, additional, Murphy, Alexander I., additional, Smith, Jessica, additional, Ding, Wenge, additional, Zhao, Chengyan, additional, Chohan, Neha, additional, Okumura, Maho, additional, Rosario, Christina, additional, Ding, Yu, additional, Lindemuth, Emily, additional, Bauer, Anya M., additional, Bar, Katharine J., additional, Ambrozak, David, additional, Chao, Cara W., additional, Chuang, Gwo-Yu, additional, Geng, Hui, additional, Lin, Bob C., additional, Louder, Mark K., additional, Nguyen, Richard, additional, Zhang, Baoshan, additional, Lewis, Mark G., additional, Raymond, Donald D., additional, Doria-Rose, Nicole A., additional, Schramm, Chaim A., additional, Douek, Daniel C., additional, Roederer, Mario, additional, Kepler, Thomas B., additional, Kelsoe, Garnett, additional, Mascola, John R., additional, Kwong, Peter D., additional, Korber, Bette T., additional, Harrison, Stephen C., additional, Haynes, Barton F., additional, Hahn, Beatrice H., additional, and Shaw, George M., additional

Published
2021
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18. Heightened resistance to type 1 interferons characterizes HIV-1 at transmission and following analytical treatment interruption

Author

Gondim, Marcos V. P., primary, Sherrill-Mix, Scott, additional, Bibollet-Ruche, Frederic, additional, Russell, Ronnie M., additional, Trimboli, Stephanie, additional, Smith, Andrew G., additional, Li, Yingying, additional, Liu, Weimin, additional, Avitto, Alexa N., additional, DeVoto, Julia, additional, Connell, Jesse, additional, Fenton-May, Angharad E., additional, Pellegrino, Pierre, additional, Williams, Ian, additional, Papasavvas, Emmanouil, additional, Lorenzi, Julio C. C., additional, Salantes, D. Brenda, additional, Mampe, Felicity, additional, Monroy, M. Alexandra, additional, Cohen, Yehuda Z., additional, Heath, Sonya, additional, Saag, Michael S., additional, Montaner, Luis J., additional, Collman, Ronald G., additional, Siliciano, Janet M., additional, Siliciano, Robert F., additional, Plenderleith, Lindsey, additional, Sharp, Paul M., additional, Caskey, Marina, additional, Nussenzweig, Michel C., additional, Shaw, George M., additional, Borrow, Persephone, additional, Bar, Katharine J., additional, and Hahn, Beatrice H., additional

Published
2020
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19. Recapitulation of HIV-1 Env-Antibody Coevolution in Macaques Leading to Neutralization Breadth

Author

Roark, Ryan S., primary, Li, Hui, additional, Williams, Wilton B., additional, Chug, Hema, additional, Mason, Rosemarie D., additional, Gorman, Jason, additional, Wang, Shuyi, additional, Lee, Fang-Hua, additional, Rando, Juliette, additional, Bonsignori, Mattia, additional, Hwang, Kwan-Ki, additional, Saunders, Kevin O., additional, Wiehe, Kevin, additional, Moody, M. Anthony, additional, Hraber, Peter T., additional, Wagh, Kshitij, additional, Giorgi, Elena E., additional, Russell, Ronnie M., additional, Bibollet-Ruche, Frederic, additional, Liu, Weimin, additional, Connell, Jesse, additional, Smith, Andrew G., additional, DeVoto, Julia, additional, Murphy, Alexander I., additional, Smith, Jessica, additional, Ding, Wenge, additional, Zhao, Chengyan, additional, Chohan, Neha, additional, Okumura, Maho, additional, Rosario, Christina, additional, Ding, Yu, additional, Lindemuth, Emily, additional, Bauer, Anya M., additional, Bar, Katharine J., additional, Ambrozak, David, additional, Chao, Cara W., additional, Chuang, Gwo-Yu, additional, Geng, Hui, additional, Lin, Bob C., additional, Louder, Mark K., additional, Nguyen, Richard, additional, Zhang, Baoshan, additional, Lewis, Mark G., additional, Raymond, Donald, additional, Doria-Rose, Nicole A., additional, Schramm, Chaim A., additional, Douek, Daniel C., additional, Roederer, Mario, additional, Kepler, Thomas B., additional, Kelsoe, Garnett, additional, Mascola, John R., additional, Kwong, Peter D., additional, Korber, Bette T., additional, Harrison, Stephen C., additional, Haynes, Barton F., additional, Hahn, Beatrice H., additional, and Shaw, George M., additional

Published
2020
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20. Predictors of Nonseroconversion after SARS-CoV-2 Infection.

Author

Weimin Liu, Russell, Ronnie M., Bibollet-Ruche, Frederic, Skelly, Ashwin N., Sherrill-Mix, Scott, Freeman, Drew A., Stoltz, Regina, Lindemuth, Emily, Fang-Hua Lee, Sterrett, Sarah, Bar, Katharine J., Erdmann, Nathaniel, Gouma, Sigrid, Hensley, Scott E., Ketas, Thomas, Cupo, Albert, Portillo, Victor M. Cruz, Moore, John P., Bieniasz, Paul D., and Hatziioannou, Theodora

Abstract

Not all persons recovering from severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection develop SARS-CoV-2-specific antibodies. We show that nonseroconversion is associated with younger age and higher reverse transcription PCR cycle threshold values and identify SARS-CoV-2 viral loads in the nasopharynx as a major correlate of the systemic antibody response. [ABSTRACT FROM AUTHOR]

Published
2021
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21. Viral Genetics Modulate Orolabial Herpes Simplex Virus Type 1 Shedding in Humans

Author

Ramchandani, Meena S, primary, Jing, Lichen, additional, Russell, Ronnie M, additional, Tran, Tran, additional, Laing, Kerry J, additional, Magaret, Amalia S, additional, Selke, Stacy, additional, Cheng, Anqi, additional, Huang, Meei-Li, additional, Xie, Hong, additional, Strachan, Eric, additional, Greninger, Alex L, additional, Roychoudhury, Pavitra, additional, Jerome, Keith R, additional, Wald, Anna, additional, and Koelle, David M, additional

Published
2018
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22. Targeted In VivoGeneration of CAR T and NK Cells Utilizing an Engineered Lentiviral Vector Platform

Author

Andorko, James I, Russell, Ronnie M, Schnepp, Bruce C, Singh, Ramesh, Boral, Debasish, O'Malley, Thomas, Kuri-Cervantes, Leticia, Medi, Muneeswara B, Culp, Timothy D, and Johnson, Philip R

Abstract

CAR T cell therapies have revolutionized the treatment of B cell malignancies. Ex vivoCAR manufacturing is complex, costly, and cumbersome, prompting efforts that develop shorter manufacturing and allogeneic or “universal donor” strategies. Conventional ex vivoCAR T cell therapy is not widely accessible and financial burdens to patients further hinder access to care. Furthermore, recent data show that CAR T product efficacy is correlated with reduced ex vivomanipulation of cells.

Published
2023
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23. CHIIMP: An automated high‐throughput microsatellite genotyping platform reveals greater allelic diversity in wild chimpanzees

Author

Barbian, Hannah J., primary, Connell, Andrew Jesse, additional, Avitto, Alexa N., additional, Russell, Ronnie M., additional, Smith, Andrew G., additional, Gundlapally, Madhurima S., additional, Shazad, Alexander L., additional, Li, Yingying, additional, Bibollet‐Ruche, Frederic, additional, Wroblewski, Emily E., additional, Mjungu, Deus, additional, Lonsdorf, Elizabeth V., additional, Stewart, Fiona A., additional, Piel, Alexander K., additional, Pusey, Anne E., additional, Sharp, Paul M., additional, and Hahn, Beatrice H., additional

Published
2018
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24. Extensive CD4 and CD8 T-cell cross-reactivity between alphaherpesviruses1

Author

Jing, Lichen, Laing, Kerry J., Dong, Lichun, Russell, Ronnie M., Barlow, Russell S., Haas, Juergen G., Ramchandani, Meena S., Johnston, Christine, Buus, Soren, Redwood, Alec J., White, Katie D., Mallal, Simon A., Phillips, Elizabeth J., Posavad, Christine M., Wald, Anna, and Koelle, David M.

Subjects
CD4-Positive T-Lymphocytes, Antigen Presentation, integumentary system, viruses, Herpesvirus 2, Human, Receptors, Antigen, T-Cell, virus diseases, Epitopes, T-Lymphocyte, Herpesviridae Infections, Herpesvirus 1, Human, Alphaherpesvirinae, CD8-Positive T-Lymphocytes, Cross Reactions, Article, Viral Proteins, T-Lymphocyte Subsets, Cytokines, Humans, Peptides, Antigens, Viral
Abstract

The Alphaherpesvirinae subfamily includes HSV types 1 and 2 and the sequence-divergent pathogen varicella zoster virus (VZV). T cells, controlled by TCR and HLA molecules that tolerate limited epitope amino acid variation, might cross-react between these microbes. We show that memory PBMC expansion with either HSV or VZV enriches for CD4 T cell lines that recognize the other agent at the whole-virus, protein, and peptide levels, consistent with bidirectional cross-reactivity. HSV-specific CD4 T cells recovered from HSV-seronegative persons can be explained, in part, by such VZV cross-reactivity. HSV-1-reactive CD8 T cells also cross-react with VZV-infected cells, full-length VZV proteins, and VZV peptides, as well as kill VZV-infected dermal fibroblasts. Mono- and cross-reactive CD8 T cells use distinct TCRB CDR3 sequences. Cross-reactivity to VZV is reconstituted by cloning and expressing TCRA/TCRB receptors from T cells that are initially isolated using HSV reagents. Overall, we define 13 novel CD4 and CD8 HSV-VZV cross-reactive epitopes and strongly imply additional cross-reactive peptide sets. Viral proteins can harbor both CD4 and CD8 HSV/VZV cross-reactive epitopes. Quantitative estimates of HSV/VZV cross-reactivity for both CD4 and CD8 T cells vary from 10 to 50%. Based on these findings, we hypothesize that host herpesvirus immune history may influence the pathogenesis and clinical outcome of subsequent infections or vaccinations for related pathogens and that cross-reactive epitopes and TCRs may be useful for multi-alphaherpesvirus vaccine design and adoptive cellular therapy.

Published
2016

25. Worldwide circulation of HSV-2 × HSV-1 recombinant strains

Author

Koelle, David M., primary, Norberg, Peter, additional, Fitzgibbon, Matthew P., additional, Russell, Ronnie M., additional, Greninger, Alex L., additional, Huang, Meei-Li, additional, Stensland, Larry, additional, Jing, Lichen, additional, Magaret, Amalia S., additional, Diem, Kurt, additional, Selke, Stacy, additional, Xie, Hong, additional, Celum, Connie, additional, Lingappa, Jairam R., additional, Jerome, Keith R., additional, Wald, Anna, additional, and Johnston, Christine, additional

Published
2017
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26. Viral Genetics Modulate Orolabial Herpes Simplex Virus Type 1 Shedding in Humans.

Author

Ramchandani, Meena S, Jing, Lichen, Russell, Ronnie M, Tran, Tran, Laing, Kerry J, Magaret, Amalia S, Selke, Stacy, Cheng, Anqi, Huang, Meei-Li, Xie, Hong, Strachan, Eric, Greninger, Alex L, Roychoudhury, Pavitra, Jerome, Keith R, Wald, Anna, and Koelle, David M

Subjects
VIRAL shedding, HUMAN herpesvirus 1
Abstract

Background Orolabial herpes simplex virus type 1 (HSV-1) infection has a wide spectrum of severity in immunocompetent persons. To study the role of viral genotype and host immunity, we characterized oral HSV-1 shedding rates and host cellular response, and genotyped viral strains, in monozygotic (MZ) and dizygotic (DZ) twins. Methods A total of 29 MZ and 22 DZ HSV-1–seropositive twin pairs were evaluated for oral HSV-1 shedding for 60 days. HSV-1 strains from twins were genotyped as identical or different. CD4+ T-cell responses to HSV-1 proteins were studied. Results The median per person oral HSV shedding rate was 9% of days that a swab was obtained (mean, 10.2% of days). A positive correlation between shedding rates was observed within all twin pairs, and in the MZ and DZ twins. In twin subsets with sufficient HSV-1 DNA to genotype, 15 had the same strain and 14 had different strains. Viral shedding rates were correlated for those with the same but not different strains. The median number of HSV-1 open reading frames recognized per person was 16. The agreement in the CD4+ T-cell response to specific HSV-1 open reading frames was greater between MZ twins than between unrelated persons (P =.002). Conclusion Viral strain characteristics likely contribute to oral HSV-1 shedding rates. [ABSTRACT FROM AUTHOR]

Published
2019
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27. Extensive CD4 and CD8 T Cell Cross-Reactivity between Alphaherpesviruses

Author

Jing, Lichen, primary, Laing, Kerry J., additional, Dong, Lichun, additional, Russell, Ronnie M., additional, Barlow, Russell S., additional, Haas, Juergen G., additional, Ramchandani, Meena S., additional, Johnston, Christine, additional, Buus, Soren, additional, Redwood, Alec J., additional, White, Katie D., additional, Mallal, Simon A., additional, Phillips, Elizabeth J., additional, Posavad, Christine M., additional, Wald, Anna, additional, and Koelle, David M., additional

Published
2016
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29. Extensive CD4 and CD8 T Cell Cross-Reactivity between Alphaherpesviruses.

Author

Lichen Jing, Laing, Kerry J., Liehun Dong, Russell, Ronnie M., Barlow, Russell S., Haas, Juergen G., Ramchandani, Meena S., Johnston, Christine, Buus, Soren, Redwood, Alec J., White, Katie D., Mallal, Simon A., Phillips, Elizabeth J., Posavad, Christine M., Wald, Anna, and Koelle, David M.

Subjects
*BLOOD cells, *HERPES simplex virus, *VARICELLA-zoster virus, *CD4 antigen, *T cells, *CROSS reactions (Immunology), *FIBROBLASTS, *VIRAL proteins
Abstract

The Alphaherpesvirinae subfamily includes HSV types 1 and 2 and the sequence-divergent pathogen varicella zoster virus (VZV). T cells, controlled by TCR and HLA molecules that tolerate limited epitope amino acid variation, might cross-react between these microbes. We show that memory PBMC expansion with either HSV or VZV enriches for CD4 T cell lines that recognize the other agent at the whole-virus, protein, and peptide levels, consistent with bidirectional cross-reactivity. HSV-specific CD4 T cells recovered from HSV-seronegative persons can be explained, in part, by such VZV cross-reactivity. HSV-1-reaetive CD8 T cells also cross-react with VZV-infected cells, full-length VZV proteins, and VZV peptides, as well as kill VZV-infected dermal fibroblasts. Mono- and cross-reactive CD8 T cells use distinct TCRB CDR3 sequences. Cross-reactivity to VZV is reconstituted by cloning and expressing TCRA7TCRB receptors from T cells that are initially isolated using HSV reagents. Overall, we define 13 novel CD4 and CD8 HSV-VZV cross-reactive epitopes and strongly imply additional cross-reactive peptide sets. Viral proteins can harbor both CD4 and CD8 HSV/VZV cross-reactive epitopes. Quantitative estimates of HSV/VZV cross-reactivity for both CD4 and CD8 T cells vary from 10 to 50%. Based on these findings, we hypothesize that host herpesvirus immune historv may influence the pathogenesis and clinical outcome of subsequent infections or vaccinations for related pathogens and that cross-reactive epitopes and TCRs may be useful for multi-alphaherpesvirus vaccine design and adoptive cellular therapy. [ABSTRACT FROM AUTHOR]

Published
2016
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30. Convalescent plasma-mediated resolution of COVID-19 in a patient with humoral immunodeficiency.

Author

Honjo K, Russell RM, Li R, Liu W, Stoltz R, Tabengwa EM, Hua Y, Prichard L, Kornbrust AN, Sterrett S, Marques MB, Lima JL, Lough CM, McCarty TP, Ketas TJ, Hatziioannou T, Bieniasz PD, Redden DT, Moore JP, Goepfert PA, Heath SL, Hahn BH, and Davis RS

Subjects
Aged, Antibodies, Neutralizing administration & dosage, COVID-19 complications, COVID-19 pathology, COVID-19 virology, Female, Humans, Immunization, Passive, Immunocompromised Host, Leukemia, Lymphocytic, Chronic, B-Cell complications, Leukemia, Lymphocytic, Chronic, B-Cell pathology, Lung diagnostic imaging, SARS-CoV-2 isolation & purification, Severity of Illness Index, Tomography, X-Ray Computed, COVID-19 Serotherapy, COVID-19 therapy
Abstract

Convalescent plasma (CP) is widely used to treat COVID-19, but without formal evidence of efficacy. Here, we report the beneficial effects of CP in a severely ill COVID-19 patient with prolonged pneumonia and advanced chronic lymphocytic leukemia (CLL), who was unable to generate an antiviral antibody response of her own. On day 33 after becoming symptomatic, the patient received CP containing high-titer (ID 50 > 5,000) neutralizing antibodies (NAbs), defervesced, and improved clinically within 48 h and was discharged on day 37. Hence, when present in sufficient quantities, NAbs to SARS-CoV-2 have clinical benefit even if administered relatively late in the disease course. However, analysis of additional CP units revealed widely varying NAb titers, with many recipients exhibiting endogenous NAb responses far exceeding those of the administered units. To obtain the full therapeutic benefits of CP immunotherapy, it will thus be important to determine the neutralizing activity in both CP units and transfusion candidates., Competing Interests: R.S.D. and K.H. have submitted an intellectual property disclosure related to the ACE2/RBD assay., (© 2020 The Author(s).)

Published
2020
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31. SARS-CoV-2-specific peripheral T follicular helper cells correlate with neutralizing antibodies and increase during convalescence.

Author

Boppana S, Qin K, Files JK, Russell RM, Stoltz R, Bibollet-Ruche F, Bansal A, Erdmann N, Hahn BH, and Goepfert P

Abstract

T-cell immunity is likely to play a role in protection against SARS-CoV-2 by helping generate neutralizing antibodies. We longitudinally studied CD4 T-cell responses to the M, N, and S structural proteins of SARS-CoV-2 in 21 convalescent individuals. Within the first two months following symptom onset, a majority of individuals (81%) mount at least one CD4 T-cell response, and 48% of individuals mount detectable SARS-CoV-2-specific peripheral T follicular helper cells (pTfh, defined as CXCR5+PD1+ CD4 T cells). SARS-CoV-2-specific pTfh responses across all three protein specificities correlate with antibody neutralization with the strongest correlation observed for S protein-specific responses. When examined over time, pTfh responses increase in frequency and magnitude in convalescence, and robust responses with magnitudes greater than 5% were detected only at the second convalescent visit, an average of 38 days post-symptom onset. These data deepen our understanding of antigen-specific pTfh responses in SARS-CoV-2 infection, suggesting that M and N protein-specific pTfh may also assist in the development of neutralizing antibodies and that pTfh response formation may be delayed in SARS-CoV-2 infection.

Published
2020
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