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24 results on '"Schoelz, James E."'

7. Report of the turnip aphid, Lipaphis erysimi (Kaltenbach, 1843) from Missouri, USA

Author

Adhab Mustafa A. and Schoelz James E.

Subjects
Cauliflower mosaic virus (CaMV), Lipaphis erysimi, turnip aphid, virus vector, Plant culture, SB1-1110
Abstract

The turnip aphid, Lipaphis erysimi (Kaltenbach, 1843), is one of the most destructive pests in the United States. It has been reported in 33 states, but had not been reported in the state of Missouri. In this study we report this species for the first time in Missouri.

Published
2015
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8. ICTV Virus Taxonomy Profile: Caulimoviridae

Author

Teycheney, Pierre-Yves, primary, Geering, Andrew D. W., additional, Dasgupta, Idranil, additional, Hull, Roger, additional, Kreuze, Jan F., additional, Lockhart, Ben, additional, Muller, Emmanuelle, additional, Olszewski, Neil, additional, Pappu, Hanu, additional, Pooggin, Mikhail M., additional, Richert-Pöggeler, Katja R., additional, Schoelz, James E., additional, Seal, Susan, additional, Stavolone, Livia, additional, Umber, Marie, additional, and Report Consortium, ICTV, additional

Published
2020
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9. Erratum to “Plant SNAREs SYP22 and SYP23 interact with Tobacco mosaic virus 126 kDa protein and SYP2s are required for normal local virus accumulation and spread” [Virology 547 (2020) 57–71].

Author

Ibrahim, Amr, primary, Yang, Xiaohua, additional, Liu, Chengke, additional, Cooper, Kimberly D., additional, Bishop, Bethany A., additional, Zhu, Min, additional, Kwon, Soonil, additional, Schoelz, James E., additional, and Nelson, Richard S., additional

Published
2020
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10. Cauliflower Mosaic Virus TAV, a Plant Virus Protein That Functions like Ribonuclease H1 and is Cytotoxic to Glioma Cells

Author

Turri, Valentina, primary, Latinovic, Olga S., additional, Bonafè, Massimiliano, additional, Toyang, Ngeh, additional, Parigi, Maria, additional, Calassanzio, Matteo, additional, Martelli, Pier Luigi, additional, Vagheggini, Alessandro, additional, Abbati, Giulia, additional, Sarnelli, Anna, additional, Casadio, Rita, additional, Ratti, Claudio, additional, Massi, Paola, additional, Schoelz, James E., additional, Salvato, Maria S., additional, Piccinini, Filippo, additional, and Martinelli, Giovanni, additional

Published
2020
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11. ICTV Virus Taxonomy Profile: Caulimoviridae

Author

Teycheney, Pierre-Yves, Geering, Andrew D., Dasgupta, Indranil, Hull, Roger, Kreuze, Jan, Lockhart, Benham E.L., Muller, Emmanuelle, Olszewski, Neil E., Pappu, Hanu R., Pooggin, Mikhail, Richert-Pöggeler, Katja R., Schoelz, James E., Seal, Susan, Stavolone, Livia, Umber, Marie, ICTV Report Consortium, Teycheney, Pierre-Yves, Geering, Andrew D., Dasgupta, Indranil, Hull, Roger, Kreuze, Jan, Lockhart, Benham E.L., Muller, Emmanuelle, Olszewski, Neil E., Pappu, Hanu R., Pooggin, Mikhail, Richert-Pöggeler, Katja R., Schoelz, James E., Seal, Susan, Stavolone, Livia, Umber, Marie, and ICTV Report Consortium

Abstract

Caulimoviridae is a family of non-enveloped reverse-transcribing plant viruses with non-covalently closed circular dsDNA genomes of 7.1–9.8 kbp in the order Ortervirales. They infect a wide range of monocots and dicots. Some viruses cause economically important diseases of tropical and subtropical crops. Transmission occurs through insect vectors (aphids, mealybugs, leafhoppers, lace bugs) and grafting. Activation of infectious endogenous viral elements occurs in Musa balbisiana, Petunia hybrida and Nicotiana edwardsonii. However, most endogenous caulimovirids are not infectious. This is a summary of the International Committee on Taxonomy of Viruses (ICTV) Report on the family Caulimoviridae, which is available at ictv.global/report/caulimoviridae.

Published
2020

15. Creation of new order, Ortervirales, for 5 families of reverse-transcribing viruses

Author

Krupovic, Mart, Harrach, Balázs, Sabanadzovic, Sead, Sanfaçon, Hélène, Koonin, Eugene V., Kuhn, Jens H., Johnson, Welkin, Blomberg, Jonas, Coffin, John, Fan, Hung, Gifford, Robert, Lindemann, Dirk, Mayer, Jens, Stoye, Jonathan, Tristem, Michael, Teycheney, Pierre-Yves, Dasgupta, Idranil, Geering, Andrew, Hull, Roger, Kreuze, Jan F., Lockhart, Ben, Muller, Emmanuelle, Olszewski, Neil, Pappu, Hanu, Pooggin, Mikhail, Richert-Pöggeler, Katja, Schoelz, James E., Seal, Susan, and Stavolone, Livia

Subjects
S1
Published
2017

18. Ortervirales: New Virus Order Unifying Five Families of Reverse-Transcribing Viruses

Author

Krupovic, Mart, primary, Blomberg, Jonas, additional, Coffin, John M., additional, Dasgupta, Indranil, additional, Fan, Hung, additional, Geering, Andrew D., additional, Gifford, Robert, additional, Harrach, Balázs, additional, Hull, Roger, additional, Johnson, Welkin, additional, Kreuze, Jan F., additional, Lindemann, Dirk, additional, Llorens, Carlos, additional, Lockhart, Ben, additional, Mayer, Jens, additional, Muller, Emmanuelle, additional, Olszewski, Neil E., additional, Pappu, Hanu R., additional, Pooggin, Mikhail M., additional, Richert-Pöggeler, Katja R., additional, Sabanadzovic, Sead, additional, Sanfaçon, Hélène, additional, Schoelz, James E., additional, Seal, Susan, additional, Stavolone, Livia, additional, Stoye, Jonathan P., additional, Teycheney, Pierre-Yves, additional, Tristem, Michael, additional, Koonin, Eugene V., additional, and Kuhn, Jens H., additional

Published
2018
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19. Ortervirales : New Virus Order Unifying Five Families of Reverse-Transcribing Viruses

Author

Krupovic, Mart, Blomberg, Jonas, Coffin, John M., Dasgupta, Indranil, Fan, Hung, Geering, Andrew D., Gifford, Robert, Harrach, Balazs, Hull, Roger, Johnson, Welkin, Kreuze, Jan F., Lindemann, Dirk, Llorens, Carlos, Lockhart, Ben, Mayer, Jens, Muller, Emmanuelle, Olszewski, Neil E., Pappu, Hanu R., Pooggin, Mikhail M., Richert-Poeggeler, Katja R., Sabanadzovic, Sead, Sanfacon, Helene, Schoelz, James E., Seal, Susan, Stavolone, Livia, Stoye, Jonathan P., Teycheney, Pierre-Yves, Tristem, Michael, Koonin, Eugene V., Kuhn, Jens H., Krupovic, Mart, Blomberg, Jonas, Coffin, John M., Dasgupta, Indranil, Fan, Hung, Geering, Andrew D., Gifford, Robert, Harrach, Balazs, Hull, Roger, Johnson, Welkin, Kreuze, Jan F., Lindemann, Dirk, Llorens, Carlos, Lockhart, Ben, Mayer, Jens, Muller, Emmanuelle, Olszewski, Neil E., Pappu, Hanu R., Pooggin, Mikhail M., Richert-Poeggeler, Katja R., Sabanadzovic, Sead, Sanfacon, Helene, Schoelz, James E., Seal, Susan, Stavolone, Livia, Stoye, Jonathan P., Teycheney, Pierre-Yves, Tristem, Michael, Koonin, Eugene V., and Kuhn, Jens H.

Published
2018
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20. Creation of new order, Ortervirales, for 5 families of reverse-transcribing viruses

Author

Kuhn, Jens H., Krupovic, Mart, Harrach, Balázs, Sabanadzovic, Sead, Sanfaçon, Hélène, Koonin, Eugene V., Welkin Johnson, Blomberg, Jonas, Coffin, John, Fan, Hung, Gifford, Robert, Lindemann, Dirk, Mayer, Jens, Stoye, Jonathan, Tristem, Michael, Pierre-Yves Teycheney, Idranil Dasgupta, Geering, Andrew, Hull, Roger, Kreuze, Jan F., Lockhart, Ben, Muller, Emmanuelle, Olszewski, Neil, Hanu Pappu, Pooggin, Mikhail, Richert-Pöggeler, Katja, Schoelz, James E., Seal, Susan, and Stavolone, Livia

Published
2017
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23. Cauliflower Mosaic Virus TAV, a Plant Virus Protein That Functions like Ribonuclease H1 and is Cytotoxic to Glioma Cells

Author

Matteo Calassanzio, Maria Parigi, Rita Casadio, Pier Luigi Martelli, Alessandro Vagheggini, James E. Schoelz, Anna Sarnelli, Maria S. Salvato, P. Massi, Olga Latinovic, Giovanni Martinelli, Massimiliano Bonafè, Valentina Turri, Giulia Abbati, Filippo Piccinini, Ngeh J. Toyang, Claudio Ratti, Turri, Valentina, Latinovic, Olga S., Bonafè, Massimiliano, Toyang, Ngeh, Parigi, Maria, Calassanzio, Matteo, Martelli, Pier Luigi, Vagheggini, Alessandro, Abbati, Giulia, Sarnelli, Anna, Casadio, Rita, Ratti, Claudio, Massi, Paola, Schoelz, James E., Salvato, Maria S., Piccinini, Filippo, and Martinelli, Giovanni

Subjects
0106 biological sciences, 0301 basic medicine, Article Subject, RNase P, Ribonuclease H, 01 natural sciences, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Viral Proteins, Caulimovirus, Plant virus, Cell Line, Tumor, Ribonuclease H1, Cytotoxic T cell, Humans, Ribonuclease, General Immunology and Microbiology, biology, Cytotoxins, fungi, RNA, food and beverages, General Medicine, Transfection, Glioma, Glioma Cells, biology.organism_classification, Virology, 030104 developmental biology, HEK293 Cells, Virion assembly, biology.protein, Medicine, Cauliflower mosaic virus, Cauliflower Mosaic Viru, 010606 plant biology & botany, Research Article
Abstract

Recent comparisons between plant and animal viruses reveal many common principles that underlie how all viruses express their genetic material, amplify their genomes, and link virion assembly with replication. Cauliflower mosaic virus (CaMV) is not infectious for human beings. Here, we show that CaMV transactivator/viroplasmin protein (TAV) shares sequence similarity with and behaves like the human ribonuclease H1 (RNase H1) in reducing DNA/RNA hybrids detected with S9.6 antibody in HEK293T cells. We showed that TAV is clearly expressed in the cytosol and in the nuclei of transiently transfected human cells, similar to its distribution in plants. TAV also showed remarkable cytotoxic effects in U251 human glioma cells in vitro. These characteristics pave the way for future analysis on the use of the plant virus protein TAV, as an alternative to human RNAse H1 during gene therapy in human cells.

Published
2020

24. A Novel Assay Based on Confocal Microscopy to Test for Pathogen Silencing Suppressor Functions.

Author

Adhab M and Schoelz JE

Subjects
DNA-Binding Proteins genetics, Green Fluorescent Proteins metabolism, Suppression, Genetic, Nicotiana virology, Viral Proteins genetics, Agrobacterium physiology, DNA-Binding Proteins metabolism, Microscopy, Confocal methods, Plant Diseases virology, RNA Interference, Nicotiana cytology, Nicotiana genetics, Viral Proteins metabolism
Abstract

In plants, RNA silencing is an important mechanism for gene regulation and defense that is targeted by proteins of viral pathogens effecting silencing suppression. In this chapter we describe a new assay to probe silencing suppressor activity using Agrobacterium infiltration of Nicotiana benthamiana and confocal microscopy. The key element in this assay involves the use of a reporter construct that is transiently expressed at a much lower level than free GFP, and this increases the sensitivity of detection of weak silencing suppressors such as the P6 protein of Cauliflower mosaic virus. Although initially developed for virus silencing suppressors, this technique could also prove valuable to characterize the potential for weak silencing suppressors in the effector repertoires of fungi, bacteria, nematodes, and oomycetes.

Published
2019
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