1. Construction of an ATP-Activated Y-Shape DNA Probe for Smart miRNA Imaging in Living Cells.
- Author
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Zhong, Wukun, Zheng, Yanlin, Huang, Lei, Xing, Chao, and Lu, Chunhua
- Subjects
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DNA probes , *MICRORNA , *CELL imaging , *ENDOCYTOSIS , *MOLECULAR probes , *GENE expression , *FOLIC acid , *APTAMERS - Abstract
A stringent DNA probe to profile microRNA (miRNA) expression within a specific cell remains a key challenge in biology. To address this issue, an intracellular ATP-activated Y-DNA probe for accurate imaging of miRNA in living cells was designed. Y-DNA was based on the fabrication of tripartite function modules, which consisted of a folate (FA)-modified targeting module, an ATP aptamer-sealed driver, and a miRNA sensing module. The Y-DNA probe could be specifically activated by ATP after it efficiently internalized into FA-receptor-overexpressed cells based on caveolar-mediated endocytosis, leading to the activation of the miRNA sensing module. The activated Y-DNA probe allowed for the imaging of miRNA in living cells with high sensitivity. The design of the ATP-activated Y-DNA sensor opens the door for bioorthogonal miRNA imaging and promotes the development of various responsive DNA molecular probes with enhanced anti-interference ability for clinical diagnosis. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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