1. 3-MCPD exposure enhances ovarian fibrosis and reduces oocyte quality in mice.
- Author
-
He, Quan-Kuo, Li, Yan-Ping, Xu, Zhi-Ran, Wei, Wen-Bo, Qiao, Feng-Xin, Sun, Ming-Xin, Liu, Yue-Cen, Chen, Yan-Zhu, Wang, Hai-Long, Qi, Zhong-Quan, and Liu, Yu
- Subjects
FIBROSIS ,REACTIVE oxygen species ,GRANULOSA cells ,IRON ions ,OVARIAN follicle ,OVUM ,GASTRIC lavage - Abstract
3-monochloro-1,2-propanediol (3-MCPD) is a food contaminant believed to be harmful to human health. Previous studies showed that 3-MCPD exerts toxic effects in multiple tissues, but whether 3-MCPD affects female reproductive function remained unknown. Here, using mouse gastric lavage models, we report that 3-MCPD exposure for four weeks affected body growth, decreased the ovary/body weight ratio, and increased atretic follicle numbers. Expression levels of follicular development-related factors decreased. Further studies found that ovaries from 3-MCPD exposed mice had activated the Transforming Growth Factor-β (TGF-β) signaling pathway and promoted ovarian fibrosis. Increased TNF-α, IL-1 and NF-κB expression also indicated the occurrence of ovarian inflammation. Exposure to 3-MCPD stimulated the caspase pathway and enhanced granulosa cell apoptosis. Consistent with disrupted ovarian homeostasis, 3-MCPD exposure interfered with mitochondrial function, generated more reactive oxygen species, increased ferrous ion and lipid peroxidation levels, and resulted in decreased oocyte development potential. Collectively, these findings indicated that 3-MCPD exposure induced ovarian inflammation and fibrosis, and caused disorders of mitochondrial function and ferrous ion homeostasis in oocytes, which consequently disturbed follicle maturation and reduced oocyte quality. [Display omitted] • 3-MCPD exposure impaires mouse follicular development. • 3-MCPD exposure induces ovary fibrosis, inflammation and apoptosis. • 3-MCPD exposure decrease oocyte quality through oxidative stress. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF