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Harar (Terminalia chebula) and soybean (Glycine max) are the potential tree and agricultural crops of subtropical region of Himachal Pradesh, India, however the potential of these crops under agroforestry system has not been explored both from ecological and economical point of view. The objective of this paper was to determine the effect of harar–soybean combination on soil chemical properties and to analyse the economics of the agroforestry system as compared to the open system. Soybean was grown under harar trees as per the recommended package of practices. Before sowing and after harvesting, soil samples were collected from four directions (East, West, North and South) at three distances (1, 2 and 3 m) from harar trees and open cropping system (sole soybean) for determining chemical properties (pH, electrical conductivity and organic carbon) and nutrient dynamics (nitrogen, phosphorus and potassium). In addition, the soil at two depths (0–15 cm and 15–30 cm) under trees at 2 m distance and in open was analyzed by GC–MS before and after cropping for the presence of allelochemicals that may affect crop yield. The economics of tree-crop combination was worked out and compared with the sole cropping system. The soil chemical properties and available nutrients in soil exhibited maximum values in the north direction. Except available phosphorus content, values of all the studied properties of soil increased with an increase in the distance of soybean crop from harar. It was also observed that values of soil chemical properties and nutrient contents increased significantly after intercropping in comparison to those before intercropping soybean. A substantial increase in soil properties was observed under the agroforestry system over the sole cropping system before sowing and after harvesting soybean. It was revealed that allelochemicals viz., octadecanoic acid, 1,2-benzenedicarboxylic acid and phthalic acid which do exist in harar trees were also detected in soil under the canopy of harar trees. The total cost of cultivation (Rs. 45,028.49 ha−1), gross returns (Rs. 101,937.78 ha−1), net returns (Rs. 56,909.29 ha−1) and benefit–cost ratio (2.26) were higher under the agroforestry system (harar + soybean) over sole soybean cropping. Improvement in soil health, soybean yield and higher economic returns indicated that combination of harar and soybean can be a viable option for farmers of subtropical areas of Himachal Pradesh for the upliftment of their socioeconomic status. [ABSTRACT FROM AUTHOR]

The present study entails the usefulness of thermophilic amidase-producing bacterium in the biotransformation of benzamide to benzohydroxamic acid (BHA). A bacterium Bacillus smithii IIIMB2907 was isolated from a soil sample collected from hot springs of Manikaran, Himachal Pradesh, India. The whole cells of the bacterium displayed versatile substrate specificity by exhibiting significant activity with a diverse range of amides. In addition, amidase from thermophilic bacterium was induced by adding Ɛ-caprolactam in the mineral base media. The optimum temperature and pH of acyltransferase activity of amidase enzyme were found to be 50 °C and 7.0, respectively. Interestingly, half-life (t1/2) of this enzyme was 17.37 h at 50 °C. Bench-scale production and purification of BHA was carried out at optimized conditions which resulted in the recovery of 64% BHA with a purity of 96%. Owing to this, the reported process in the present study can be considered of immense industrial significance for the production of BHA. [ABSTRACT FROM AUTHOR]

In the recent decades, regeneration patterns of forest species have played an important role in mitigating future climate impacts. This study analysed impact of disturbances due to grazing on the regeneration status of temperate reserve forest species in Churdhar Wildlife Sanctuary, Himachal Pradesh, located in Western Himalaya of India spanning a wide range of altitudes. For analysis of vegetation, quadrat method was used to assess regeneration status and level of disturbance (grazing intensity). We found that seedling and sapling density and species richness decreased with increasing altitude. Overall, a fairly good regeneration potential was observed for tree species, and few of them reported poor regeneration exclusively due to grazing disturbances at higher altitudes. The forest growing at lower altitudes showed better regeneration than the forests growing at higher altitudes. Majority of dominant and co-dominant tree species showed a reverse j-shaped curve, but Quercus semecarpifolia and Rhododendron arboreum showed an inverse j-shaped curve. The study concluded that poor regeneration of species at higher altitude could be attributed to grazing and harsh environmental factors and, therefore, recommends adoption of suitable management measures at different phases of forest reproduction for successful forest establishment. [ABSTRACT FROM AUTHOR]