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This article shows the adequacy of the custom-built optical imaging system in the advancement investigation of obese mice. Obesity is defined as increased adipose/fatty mass resulting from a chronic imbalance between energy intake and expenditure. The in vivo investigation was performed for the tissue characterization of obese mice utilizing swept-source optical coherence tomography (SSOCT) for in situ examination and histology of delicate tissues in mice skin. It provides a noninvasive, painless visualization of the subsurface in life systems. Our SSOCT system's data is comparable to the regular invasive histology. Cross-assessment is done in various skin layers in obese mice like epidermis, papillary dermis, dermis, and fat tissue, which are likewise separated from the nonobese mice group. Histopathology results were further assessed with the obtained SSOCT results. This high precision of characterizing tissues using SSOCT helps us perform in vivo imaging and can also be used for the variable purpose of clinical practice.

To better understand any tumor, first, we need to know the characteristic features. The tumor microenvironment is that the signature helps us know the dermatological condition for biopsy. Some traditional optical imaging methods have restricted properties to visualize shallow skin highlights, and boundary data is missing to identify pathophysiology relationships of skin infections. Here, we illustrate a swept-source optical coherence tomography (SSOCT) technique that non-invasively visualizes (in-vivo) the tissue micro-structure/neoplastic changes during tumor development as optical biopsy in real-time with pathophysiological correlations in the mice model. To further validate the current study, we perform the corresponding histopathology (ex-vivo) of that tumor region. Furthermore, the scattering coefficient and the optical penetration depth analysis prove that SSOCT imaging can provide detailed findings and treatment in dermatology.