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Your search keyword '"Royce A. Wilkinson"' showing total 2 results
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2 results on '"Royce A. Wilkinson"'

1. Sequence-specific capture and concentration of viral RNA by type III CRISPR system enhances diagnostic

Author

Anna Nemudraia, Artem Nemudryi, Murat Buyukyoruk, Andrew M. Scherffius, Trevor Zahl, Tanner Wiegand, Shishir Pandey, Joseph E. Nichols, Laina N. Hall, Aidan McVey, Helen H. Lee, Royce A. Wilkinson, Laura R. Snyder, Joshua D. Jones, Kristin S. Koutmou, Andrew Santiago-Frangos, and Blake Wiedenheft

Subjects
Science
Abstract

Many viral diagnostic approaches require nucleic acid extraction and amplification prior to detection. Here, the authors develop a method based on type III CRISPR systems which allows sequence specific capture, concentration, and detection of viral RNA directly from patient samples.

Published
2022
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2. Protein-mediated genome folding allosterically enhances site-specific integration of foreign DNA into CRISPRs

Author

Andrew Santiago-Frangos, William S. Henriques, Tanner Wiegand, Colin C. Gauvin, Murat Buyukyoruk, Ava B. Graham, Royce A. Wilkinson, Lenny Triem, Kasahun Neselu, Edward T. Eng, Gabriel C. Lander, and Blake Wiedenheft

Abstract

Bacteria and archaea acquire resistance to viruses and plasmids by integrating fragments of foreign DNA into the first repeat of a CRISPR array. However, the mechanism of site-specific integration remains poorly understood. Here, we determine a 560 kDa integration complex structure that explains how Cas (Cas1-2/3) and non-Cas proteins (IHF) fold 150 base-pairs of host DNA into a U-shaped bend and a loop that protrude from Cas1-2/3 at right angles. The U-shaped bend traps foreign DNA on one face of the Cas1-2/3 integrase, while the loop places the first CRISPR repeat in the Cas1 active site. Both Cas3s rotate 100-degrees to expose DNA binding sites on either side of the Cas2 homodimer, that each bind an inverted repeat motif in the leader. Leader sequence motifs direct Cas1-2/3-mediated integration to diverse repeat sequences that have a 5’-GT.

Published
2023

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