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The superposition principle is one of the most fundamental principles of quantum mechanics. According to the Schr\"odinger equation, a physical system can be in any linear combination of its possible states. While the validity of this principle is routinely validated for microscopic systems, it is still unclear why we do not observe macroscopic objects to be in superpositions of states that can be distinguished by some classical property. Here we demonstrate the preparation of a mechanical resonator with an effective mass of 16.2 micrograms in Schr\"odinger cat states of motion, where the constituent atoms are in a superposition of oscillating with two opposite phases. We show control over the size and phase of the superposition and investigate the decoherence dynamics of these states. Apart from shedding light at the boundary between the quantum and the classical world, our results are of interest for quantum technologies, as they pave the way towards continuous-variable quantum information processing and quantum metrology with mechanical resonators.

Chronic infection of the common bacterial pathogenPseudomonas aeruginosafrequently leads to the coexistence of heterogeneous individuals to engage in several group behaviors. However, further evolution of the polymorphicP. aeruginosapopulation, including the dynamic change of social cooperation and its impact on host immune system, still remain elusive. We show that the evolution ofP. aeruginosain the patients with chronic obstructive pulmonary disease frequently selects the isolates deficient in producing the costly and sharable extracellular products for nutrient acquisition. The evolution of polymorphicP. aeruginosapopulation is mainly concentrated on modifying the adaptability oflasRmutants, which are typical cheaters in the competition of quorum-sensing-controlled extracellular proteases. Importantly,lasRmutants with varying degrees of evolution interact with wild-typeP. aeruginosain a framework termed cascaded public goods game to compete for extracellular proteases and siderophores, and thus perpetuate social cooperation under different conditions. Finally, we find that a polymorphic population comprised oflasR-intactP. aeruginosaand evolvedlasR-mutant can minimize the host immune fluctuation for persistent colonization. This study demonstrates the multistage evolution and complex interaction ofP. aeruginosain adaptation to host lungs, and provides an explanation for the success of cooperation in public goods game.

Mechanical resonators are emerging as an important new platform for quantum science and technologies. A large number of proposals for using them to store, process, and transduce quantum information motivates the development of increasingly sophisticated techniques for controlling mechanical motion in the quantum regime. By interfacing mechanical resonators with superconducting circuits, circuit quantum acoustodynamics (cQAD) can make a variety of important tools available for manipulating and measuring motional quantum states. Here we demonstrate direct measurements of the phonon number distribution and parity of nonclassical mechanical states. We do this by operating our system in the strong dispersive regime, where a superconducting qubit can be used to spectroscopically resolve phonon Fock states. These measurements are some of the basic building blocks for constructing acoustic quantum memories and processors. Furthermore, our results open the door to performing even more complex quantum algorithms using mechanical systems, such as quantum error correction and multi-mode operations.

Constructing artificial dynamic architectures inside cells to rationally interfere with organelles is emerging as an efficient strategy to regulate the behaviors and fate of cells, thus providing new routes for therapeutics. Herein, we develop an intracellular K

On the basis of the one strain-many compounds (OSMAC) strategy, two new hygromycin A derivatives (3, 4), together with six known compounds were isolated from a medicinal plant inter rhizospheric Streptomyces in Pulsatilla chinensis. The structures of 3 and 4 were elucidated using NMR and HRESIMS analyses. A plausible biosynthetic pathway for these compounds was discussed. All the compounds were evaluated for their antimicrobial and cytotoxic activities. Compound 5 exhibited potent inhibitory activity against S. aureus and B. subtilis with the MICs of 16 and 8 μg ml

Molecular docking is a structure-based and computer-aided drug design approach that plays a pivotal role in drug discovery and pharmaceutical research. AutoDock is the most widely used molecular docking tool for study of protein–ligand interactions and virtual screening. Although many tools have been developed to streamline and automate the AutoDock docking pipeline, some of them still use outdated graphical user interfaces and have not been updated for a long time. Meanwhile, some of them lack cross-platform compatibility and evaluation metrics for screening lead compound candidates. To overcome these limitations, we have developed Dockey, a flexible and intuitive graphical interface tool with seamless integration of several useful tools, which implements a complete docking pipeline covering molecular sanitization, molecular preparation, paralleled docking execution, interaction detection and conformation visualization. Specifically, Dockey can detect the non-covalent interactions between small molecules and proteins and perform cross-docking between multiple receptors and ligands. It has the capacity to automatically dock thousands of ligands to multiple receptors and analyze the corresponding docking results in parallel. All the generated data will be kept in a project file that can be shared between any systems and computers with the pre-installation of Dockey. We anticipate that these unique characteristics will make it attractive for researchers to conduct large-scale molecular docking without complicated operations, particularly for beginners. Dockey is implemented in Python and freely available at https://github.com/lmdu/dockey.

A novel actinobacterium, designated strain H8750T, was isolated from sediment sampled at Lugu Lake, southwest PR China and its polyphasic taxonomy was studied. Strain H8750T produced well-developed substrate mycelium, and formed club-shaped and hooked structures borne on the tip of the aerial mycelia. It contained meso-diaminopimelic, glucose, ribose and madurose in whole-cell hydrolysates. The predominant menaquinones were MK-9(H4), MK-9(H2) and MK-9(H6). The phospholipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, hydroxy-phosphatidylethanolamine, unidentified phospholipids and unidentified aminophospholipids. The major fatty acid (>10 %) were cis 9 C17 : 1, iso-C16 : 0 and C15 : 0. The DNA G+C content was 69.7 mol% based on the whole genome sequence. Phylogenetic analysis based on 16S rRNA gene and whole-genome sequences indicated that strain H8750T was closely related to Acrocarpospora macrocephala JCM 10982T (98.0 %), Acrocarpospora pleiomorpha JCM 10983T (97.9 %) and Acrocarpospora phusangensis DSM 45867 T (97.8 %) and formed a monophyletic clade within the genus Acrocarpospora in the phylogenetic trees. The average nucleotide identity and digital DNA–DNA hybridization values between strain H8750T and its closely related Acrocarpospora species were 79.8~87.2 % and 25.9~28.0 %, respectively, which showed that it belonged to a distinct species. Furthermore, the morphological and phenotypic characteristics allowed the isolate to be differentiated from its closely related species. Therefore, it is concluded that strain H8750T can be classified as representing a novel species of the genus Acrocarpospora , for which the name Acrocarpospora catenulata sp. nov. is proposed. The type strain is H8750T (=JCM 34849T=CICC 25116T). Moreover, based on the gene prediction results, strain H8750T may have the genetic potential to synthesize many new secondary metabolites, which further increases its bioactive value.

Recently, solid-state mechanical resonators have become a platform for demonstrating non-classical behavior of systems involving a truly macroscopic number of particles. Here, we perform the most macroscopic quantum test in a mechanical resonator to date, which probes the validity of quantum mechanics at the microgram mass scale. This is done by a direct measurement of the Wigner function of a high-overtone bulk acoustic wave resonator mode, monitoring the gradual decay of negativities over tens of microseconds. While the obtained macroscopicity of $\mu= 11.3$ is on par with state-of-the-art atom interferometers, future improvements of mode geometry and coherence times could confirm the quantum superposition principle at unprecedented scales., Comment: 5+9 pages, 2+6 figures, comments are welcome

(±)-Pyriindolin (1) with a rare molecular backbone formed by fusing a 2,2′-bipyridine nucleus into a spiro[furan-3,3′-indoline] skeleton, was isolated from the Streptomyces albolongus EA12432. The constitution and the relative configuration of (±)-1 were determined by extensive spectroscopic analyses, 13C calculation and DP4+ probability analysis. The absolute configurations of optically pure (+)-1 and (−)-1 which were obtained after a chiral high performance liquid chromatography (HPLC) separation were further identified by electronic circular dichroism (ECD) calculations. (+)- and (−)-Pyriindolins displayed moderate cytotoxicity against HCT-116 cell line with the half-maximal inhibitory concentration (IC50) values of 2.89 ± 0.17 μmol/L and 4.47 ± 0.26 μmol/L, respectively.

Bulk acoustic resonators support robust, long-lived mechanical modes, capable of coupling to various quantum systems. In separate works, such devices have achieved strong coupling to both superconducting qubits, via piezoelectricity, and optical cavities, via Brillouin interactions. In this work, we present a hybrid microwave–optical platform capable of coupling to bulk acoustic waves through cavity-enhanced piezoelectric and photoelastic interactions. The modular, tunable system achieves fully resonant and well-mode-matched interactions among a 3D microwave cavity, a high-frequency bulk acoustic resonator, and a Fabry–Perot cavity. We realize this piezo–Brillouin interaction in x-cut quartz, demonstrating the potential for strong optomechanical interactions and high cooperativity using optical cavity enhancement. We further show how this device functions as a bidirectional electro–opto–mechanical transducer, with transduction efficiency exceeding 10 − 8 , and a feasible path towards unity conversion efficiency. The high optical sensitivity and ability to apply a large resonant microwave field in this system also offers a tool for probing anomalous electromechanical couplings, which we demonstrate by investigating (nominally centrosymmetric) C a F 2 and revealing a parasitic piezoelectricity of 83 am/V. Such studies are an important topic for emerging quantum technologies, and highlight the versatility of this hybrid platform.

Bacterial species in the polymicrobial community evolve interspecific interaction relationships to adapt to the survival stresses imposed by neighbors or environmental cues. Pseudomonas aeruginosa and Staphylococcus aureus are two common bacterial pathogens frequently coisolated from patients with burns and respiratory disease. Whether the application of commonly used antibiotics influences the interaction dynamics of the two species still remains largely unexplored. By performing a series of on-plate competition assays and RNA sequencing-based transcriptional profiling, we showed that the presence of the cephalosporin antibiotic cefotaxime or the quinolone antibiotic levofloxacin at subinhibitory concentration contributes to selecting P. aeruginosa from the coculture with S. aureus by modulating the quorum-sensing (QS) system of P. aeruginosa. Specifically, a subinhibitory concentration of cefotaxime promotes the growth suppression of S. aureus by P. aeruginosa in coculture. This process may be related to the increased production of the antistaphylococcal molecule pyocyanin and the expression of lasR, which is the central regulatory gene of the P. aeruginosa QS hierarchy. On the other hand, subinhibitory concentrations of levofloxacin decrease the competitive advantage of P. aeruginosa over S. aureus by inhibiting the growth and the las QS system of P. aeruginosa. However, pqs signaling of P. aeruginosa can be activated instead to overcome S. aureus. Therefore, this study contributes to understanding the interaction dynamics of P. aeruginosa and S. aureus during antibiotic treatment and provides an important basis for studying the pathogenesis of polymicrobial infections. IMPORTANCE Increasing evidence has demonstrated the polymicrobial characteristics of most chronic infections, and the frequent communications among bacterial pathogens result in many difficulties for clinical therapy. Exploring bacterial interspecific interaction during antibiotic treatment is an emerging endeavor that may facilitate the understanding of polymicrobial infections and the optimization of clinical therapies. Here, we investigated the interaction of cocultured P. aeruginosa and S. aureus with the intervention of commonly used antibiotics in clinic. We found that the application of subinhibitory concentrations of cefotaxime and levofloxacin can select P. aeruginosa in coculture with S. aureus by modulating P. aeruginosa QS regulation to enhance the production of antistaphylococcal metabolites in different ways. This study emphasizes the role of the QS system in the interaction of P. aeruginosa with other bacterial species and provides an explanation for the persistence and enrichment of P. aeruginosa in patients after antibiotic treatment and a reference for further clinical therapy.

Background Trueperella pyogenes and Pseudomonas aeruginosa are two important bacterial pathogens closely relating to the occurrence and development of forest musk deer respiratory purulent disease. Although T. pyogenes is the causative agent of the disease, the subsequently invaded P. aeruginosa will predominate the infection by producing a substantial amount of quorum-sensing (QS)-controlled virulence factors, and co-infection of them usually creates serious difficulties for veterinary treatment. In order to find a potential compound that targets both T. pyogenes and P. aeruginosa, the antibacterial and anti-virulence capacities of 55 compounds, which have similar core structure to the signal molecules of P. aeruginosa QS system, were tested in this study by performing a series of in vitro screening experiments. Results We identified that furazolidone could significantly reduce the cell densities of T. pyogenes in mono-culture or in the co-culture with P. aeruginosa. Although the growth of P. aeruginosa could also be moderately inhibited by furazolidone, the results of phenotypic identification and transcriptomic analysis further revealed that sub-inhibitory furazolidone had remarkable inhibitory effect on the biofilm production, motility, and QS system of P. aeruginosa. Moreover, furazolidone could efficiently protect Caenorhabditis elegans models from P. aeruginosa infection under both fast-killing and slow-killing conditions. Conclusions This study reports the antibacterial and anti-virulence abilities of furazolidone on T. pyogenes and P. aeruginosa, and provides a promising strategy and molecular basis for the development of novel anti-infectious drugs to dealing with forest musk deer purulent disease, or other diseases caused by T. pyogenes and P. aeruginosa co-infection.

Red algae are the most widely cultivated macroalgae crops in aquaculture, and they are often directly ingested in a daily diet and used as raw material for food hydrocolloid extraction. The current dilemma regarding red algae exploitation involves their low added value. Here, we propose using an enzyme-assisted one-step process (ESOP) to convert homogenized Gracilariopsis lemaneiformis to high valued neoagarooligosaccharides (NAOs) and dietary fibre simultaneously. First, we optimized the hydrolysis process (the following conditions were used: 15 g wet G. lemaneiformis/150 mL buffer, 15 min homogenization and 25 U load enzyme amount) to produce 17.13 mg crude NAOs. Second, TLC, CNMR and MALDI-TOF-MS were performed, and the results indicated that NAOs may be modified by a variety of substitutions. Meanwhile, NAOs showed antioxidant activities in the DPPH, ABTS and free radical scavenging experiments at different concentrations. Third, dietary fibre concentrates were prepared and shown to have 60.78% dietary fibre, 4.19% protein, 8.43% moisture and 2.06% ash contents. Dietary fibre concentrate might have great potential in the food industry because of its physiological properties, including its thermostability at 30–1150 °C, water holding capacity (24.20 g/g) and oil holding capacity (8.26 g/g). Therefore, G. lemaneiformis was firstly converted into high value-added NAOs and dietary fibre at the same time using ESOP. This process was environmentally friendly and preserved the active compounds, and therefore provided clues for the further comprehensive use of other seaweeds or plants containing gel-like polysaccharides.