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Aim: The aim of this study was to reveal the epigenome landscape of human normal hepatocytes. Materials & methods: Cells purified from partial hepatectomy specimens of Japanese patients were subjected to whole-genome bisulfite sequencing using postbisulfite adaptor tagging, chromatin immunoprecipitation sequencing, RNA sequencing and whole-genome sequencing. Results: CHG and CHH methylations were inversely associated with gene expression. Histone modification profiles of personal differentially methylated regions (pDMRs) differed considerably among samples. pDMRs were observed around the transcription start sites of genes whose expression is reportedly regulated by CpG methylation. pDMRs were frequently observed in the vicinity of single-nucleotide variations and insertions/deletions. Conclusion: Genetic variations may induce epigenetic variations, generating individual differences in the phenotypes of normal hepatocytes through variations in expression.

This paper presents a new method for rapid assessment of the extent of annual croplands in Brazil. The proposed method applies a linear spectral mixing model (LSMM) to PROBA-V time series images to derive vegetation, soil, and shade fraction images for regional analysis. We used S10-TOC (10 days synthesis, 1 km spatial resolution, and top-of-canopy) products for Brazil and S5-TOC (five days synthesis, 100 m spatial resolution, and top-of-canopy) products for Mato Grosso State (Brazilian Legal Amazon). Using the time series of the vegetation fraction images of the whole year (2015 in this case), only one mosaic composed with maximum values of vegetation fraction was generated, allowing detecting and mapping semi-automatically the areas occupied by annual crops during the year. The results (100 m spatial resolution map) for the Mato Grosso State were compared with existing global datasets (Finer Resolution Observation and Monitoring?Global Land Cover (FROM-GLC) and Global Food Security?Support Analyses Data (GFSAD30)). Visually those maps present a good agreement, but the area estimated are not comparable since the agricultural class definition are different for those maps. In addition, we found 11.8 million ha of agricultural areas in the entire Brazilian territory. The area estimation for the Mato Grosso State was 3.4 million ha for 1 km dataset and 5.3 million ha for 100 m dataset. This difference is due to the spatial resolution of the PROBA-V datasets used. A coefficient of determination of 0.82 was found between PROBA-V 100 m and Landsat-8 OLI area estimations for the Mato Grosso State. Therefore, the proposed method is suitable for detecting and mapping annual croplands distribution operationally using PROBA-V datasets for regional analysis. Made available in DSpace on 2020-12-15T09:04:16Z (GMT). No. of bitstreams: 1 SANO-VEGETATION-FRACTION-IMAGES-DERIVED.pdf: 19445751 bytes, checksum: bfb77e6986a01be978681caf548120cb (MD5) Previous issue date: 2020

Carry out an updated meta-analysis to comprehensively search and review evidence available heretofore and derive reliable assessment of the diagnostic performances of computed tomography angiography-derived fractional flow reserve (CT-FFR) using fractional flow reserve (FFR) as the reference

In less than 15 years, the Amazon region experienced three major droughts. Links between droughts and fires have been demonstrated for the 1997/1998, 2005, and 2010 droughts. In 2010, emissions of 510 ± 120 Tg C were associated to fire alone in Amazonia. Existing approaches have, however, not yet disentangled the proportional contribution of multiple land cover sources to this total. We develop a novel integration of multisensor and multitemporal satellite-derived data on land cover, active fires, and burned area and an empirical model of fire-induced biomass loss to quantify the extent of burned areas and resulting biomass loss for multiple land covers in Mato Grosso (MT) state, southern Amazonia—the 2010 drought most impacted region. We show that 10.77% (96,855 km2) of MT burned. We estimated a gross carbon emission of 56.21 ± 22.5 Tg C from direct combustion of biomass, with an additional 29.4 ± 10 Tg C committed to be emitted in the following years due to dead wood decay. It is estimated that old-growth forest fires in the whole Brazilian Legal Amazon (BLA) have contributed to 14.81 Tg of C (11.75 Tg C to 17.87 Tg C) emissions to the atmosphere during the 2010 fire season, with an affected area of 27,555 km2. Total C loss from the 2010 fires in MT state and old-growth forest fires in the BLA represent, respectively, 77% (47% to 107%) and 86% (68.2% to 103%) of Brazil's National Plan on Climate Change annual target for Amazonia C emission reductions from deforestation.

In tropical areas, Dynamic Global Vegetation Models (DGVMs) still have deficiencies in simulating the timing of vegetation phenology. To start addressing this problem, standard Fourier-based methods are applied to aerosol screened monthly remotely sensed phenology time series (Enhanced Vegetation Index, EVI) and two major driving factors of phenology: solar radiation and precipitation (for March 2000 through December 2006 over northern South America). At 1 x 1 km scale using, power (or variance) spectra on good quality aerosol screened time series, annual cycles in EVI are detected across 58.24% of the study area, the strongest (largest amplitude) occurring in the savanna. Terra Firme forest have weak but significant annual cycles in comparison with savannas because of the heterogeneity of vegetation and nonsynchronous phenological events within 1 x 1 km scale pixels. Significant annual cycles for radiation and precipitation account for 86% and 90% of the region, respectively, with different spatial patterns to phenology. Cross-spectral analysis was used to compare separately radiation with phenology/EVI, precipitation with phenology/EVI and radiation with precipitation. Overall the majority of the Terra Firme forest appears to have radiation as the driver of phenology (either radiation is in phase or leading phenology/EVI at the annual scale). These results are in agreement with previous research, although in Acre, central and eastern Peru and northern Bolivia there is a coexistence of 'in phase' precipitation over Terra Firme forest. In contrast in most areas of savanna precipitation appears to be a driver and savanna areas experiencing an inverse (antiphase) relationship between radiation and phenology is consistent with inhibited grassland growth due to soil moisture limitation. The resulting maps provide a better spatial understanding of phenology-driver relationships offering a bench mark to parameterize ecological models.

Remote sensing data are a key tool to assess large forested areas, where limitations such as accessibility and lack of field measurements are prevalent. Here, we have analysed datasets from moderate resolution imaging spectroradiometer (MODIS) satellite measurements and field data to assess the impacts of the 2005 drought in Amazonia. We combined vegetation indices (VI) and climatological variables to evaluate the spatiotemporal patterns associated with the 2005 drought, and explore the relationships between remotely-sensed indices and forest inventory data on tree mortality. There were differences in results based on c4 and c5 MODIS products. C5 VI showed no spatial relationship with rainfall or aerosol optical depth; however, distinct regions responded significantly to the increased radiation in 2005. The increase in the Enhanced VI (EVI) during 2005 showed a significant positive relationship (P < 0.07) with the increase of tree mortality. By contrast, the normalized difference water index (NDWI) exhibited a significant negative relationship (P < 0.09) with tree mortality. Previous studies have suggested that the increase in EVI during the 2005 drought was associated with a positive response of forest photosynthesis to changes in the radiation income. We discuss the evidence that this increase could be related to structural changes in the canopy.

This introductory paper to the volume explains the DIISM problem statement and applies principles of architecture descriptions for evolutionary systems (IEEE 1471-2000) to the information infrastructure for engineering and manufacturing. In our vision, knowledge and skill chains depend on infrastructure systems fulfilling missions in three kinds of environments: the socio-industrial domain of society and its production systems as a whole, the knowledge domain for a scientific discipline, and the sectorial domain, which includes the operational entities (companies, organisational units, engineers, workers) in engineering and manufacturing. The relationships between these different domains are captured in a domain paradigm. For companies, the original scope for infrastructure systems was the factory floor and the engineering office. Recently the scopes of external collaboration and of man-system collaboration have gained importance. Within each of the four identified scopes a system can offer services to different operational levels: operations, development or engineering, and research. The dimensions of scope and service level are briefly explained in relation to the architecting of an infrastructure. Papers are grouped according to their contribution to an infrastructure scenario or to an infrastructure component.

This paper is about the requirements for an advanced factory governance system. Five capital assets are distinguished: Natural. artificial, human, social, and financial. A factory's operations involve and affect thcse fivc capital assets. To scopc workers' activities with respcct to objectives that exist for these capital asscts, this paper applies insights from Manufacturing System Design Decomposition. It is argued that in an advanccd factory govcrnance system the workers do also engage in govcrnance and management activities. We introduce the decision-object hierarchies and an extended generic activity model and explain how human profiles are derived from these. Sociotechnical systems design can be used in such a system to balance 'control by the system', and 'self-control' by the workers involved.