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Salmonella spp. are known pathogens in fish, with their presence potentially resulting from the contamination of the aquatic environment or improper handling. Accurate bacterial identification is crucial across various fields, including medicine, microbiology, and the food industry, and thus a range of techniques are available for this purpose. In this study, Salmonella spp. and other hydrogen sulphide-positive bacteria were investigated in the digestive contents of fish destined for consumption from the Atlantic area of Macaronesia. Two identification techniques were compared: the traditional API method and the MALDI-TOF MS technique. For the identification of Salmonella spp. carriers, 59 samples were processed following ISO 6579–1:2017. A total of 47 strains of Gram-negative bacilli were obtained. No Salmonella spp. isolates were detected. The most frequent genus was Enterobacter (76.50%), followed by Shewanella (10.63%). The MALDI-TOF MS technique showed a high concordance with the API technique, with 72.34% concordance at the species level. Both techniques demonstrated a high degree of concordance in the identification of Enterobacter cloacae, with 87.23% genus-level concordance and 12.76% non-concordant identifications. This study highlights the limitations of the API technique and the speed and precision of MALDI-TOF MS. The identified bacteria could pose a health risk to humans.

This technical paper provides descriptions and classification of the main semi-industrial and industrial fishing vessel types: trawlers, purse seiners, seiners, dredgers, gillnetters, trap setters, longliners, pole-and-line vessels, trollers, and multipurpose vessels. It also covers vessels supporting fishing-related activities such as fish carriers, motherships, fisheries research vessels and vessels involved in aquaculture operations. It includes illustrations of most of the vessel types and drawings of the general arrangements of selected vessels. The design, size and other characteristics of the main types of semiindustrial and industrial fishing vessels have changed significantly in recent decades, so the second edition includes several important updates with respect to the 1985 version. The vessel type classification codes employed have been updated, as has the approximate relationship between fishing vessels' length overall (LOA) and their gross tonnage (GT). The classification of the fishing vessel types follows the International Standard Statistical Classification of Fishery Vessels by Vessel Types (ISSCFV) that was endorsed by the Coordinating Working Party on Fishery Statistics (CWP) in 2019. The descriptions and classification are valid worldwide. The main purpose of this technical paper is to assist FAO Members, regional fishery bodies, as well as those working on fishery statistics and management, with updated information on vessel types and characteristics. It provides users, including non-specialists, with sufficient information to identify and classify all types of semi-industrial and industrial fishery vessels for reporting purposes. The paper also contributes to FAO's work in support of the implementation of the 1999 International Plan of Action for the Management of Fishing Capacity (IPOA-Capacity), with regard to the prevention, deterrence and elimination of illegal, unreported and unregulated (IUU) fishing. It does so by providing monitoring, control and surveillance personnel with information to identify the type of fishery vessels in terms of their licensing and authorization to carry out fishing and fishing-related operations. The paper also complements the International Standard Statistical Classification of Fishing Gear (ISSCFG) and the FAO publication Classification and illustrated definition of fishing gears. [ABSTRACT FROM AUTHOR]

Introduction: This study evaluates the feasibility of culturing the high-value marine fish Protonibea diacanthus in a polyculture system with Indian pompano (Trachinotus mookalee) using a marine cage culture setup. The study aims to determine growth performance, feed efficiency, and the potential of P. diacanthus as a candidate species for polyculture. Methods: Wild-caught P. diacanthus were reared in six high-density polyethylene sea cages (Inner Diameter: 6 m; Outer Diameter: 7 m; Depth: 4.5 m; Area: 28.29 m²; Volume: 127.29 m³). Two experimental groups were established: Group-1 with 90 individuals (average weight: 130.15 ± 6.39 g) and Group-2 with 90 individuals (average weight: 287.80 ± 16.90 g). Each group was divided into three cages (30 fish/cage) and reared in triplicate with T. mookalee (2,500 fish/cage, average weight: 31.12 ± 1.17 g). The fish were cultured for 148 days and fed a commercial diet (40% crude protein, 10% crude lipid). Growth indices and feed efficiency indices were analyzed, and growth of the fish was modeled using von Bertalanffy Growth Function (VBGF). Results: Growth performance indices, including total length gain, body weight gain, and specific growth rate, were significantly higher in Group-1 (P ≤ 0.05). Feed efficiency indices (feed conversion ratio, feed efficiency ratio, and protein efficiency ratio) did not differ significantly between the groups (P > 0.05). VBGF modeling estimated P. diacanthus asymptotic length (L∞) at 164.21 ± 3.58 cm, asymptotic weight (W∞) at 44,070.19 ± 2811 g, growth coefficient (K) at 0.30 ± 0.01 yr−1 and t₀ at -0.005 ± 0.02 yr. The growth performance index (ϕ') was calculated at 1.91, surpassing values reported for wild populations. Polyculture with P. diacanthus did not significantly affect the growth or feed utilization of T. mookalee (P > 0.05). Discussion and conclusion: The results indicate that P. diacanthus exhibits superior growth under marine cage culture conditions, with a high growth performance and compatibility in polyculture systems. These findings support the potential of P. diacanthus as a viable candidate for integration into commercial polyculture systems. [ABSTRACT FROM AUTHOR]

Simple Summary: This study addresses the issue of the detection of harmful bacteria (Salmonella and other hydrogen sulphide-positive bacteria) present in fish for consumption, which may arise from contaminated water or improper handling. The aim is to compare two methods for identifying these bacteria in the digestive content of fish from the Macaronesian region. A total of 59 samples were analysed. Salmonella was not detected, but other bacteria were identified (47), the most common being Enterobacter. This study revealed that the new MALDI-TOF MS method is faster and more accurate than the traditional API method, showing high concordance in the identification of bacteria. These findings are important because they highlight the limitations of older techniques and emphasise the importance of using more effective methods to ensure food safety. By improving the identification of potentially harmful bacteria, this research can help protect public health and improve safety standards in the food industry. Salmonella spp. are known pathogens in fish, with their presence potentially resulting from the contamination of the aquatic environment or improper handling. Accurate bacterial identification is crucial across various fields, including medicine, microbiology, and the food industry, and thus a range of techniques are available for this purpose. In this study, Salmonella spp. and other hydrogen sulphide-positive bacteria were investigated in the digestive contents of fish destined for consumption from the Atlantic area of Macaronesia. Two identification techniques were compared: the traditional API method and the MALDI-TOF MS technique. For the identification of Salmonella spp. carriers, 59 samples were processed following ISO 6579–1:2017. A total of 47 strains of Gram-negative bacilli were obtained. No Salmonella spp. isolates were detected. The most frequent genus was Enterobacter (76.50%), followed by Shewanella (10.63%). The MALDI-TOF MS technique showed a high concordance with the API technique, with 72.34% concordance at the species level. Both techniques demonstrated a high degree of concordance in the identification of Enterobacter cloacae, with 87.23% genus-level concordance and 12.76% non-concordant identifications. This study highlights the limitations of the API technique and the speed and precision of MALDI-TOF MS. The identified bacteria could pose a health risk to humans. [ABSTRACT FROM AUTHOR]

Enteric red mouth disease (ERM) or yersiniosis, is an infectious and contagious disease, that is wildly spread across the world. In Romania, trout culture is facing this serious disease that causes a high mortality among fish population, varying between 10 and 60%. The etiologic agent of the disease is the virulent bacterium Yersinia ruckeri, from the Enterobacteriaceae family. This bacterium is considered strictly pathogenic, although it can survive a few months in water and sediments. The sources of infection can be the ill fish, carriers of the pathogen, that spread the bacteria in the water through their excrements of the poorly maintained ponds, water and sediment, farm equipment, bird excrements, some mammals. The predominant factors leading to yersiniosis disease are poor fish rearing conditions (accumulations out of the accepted limits for organic substances, nitrates, oxygen deficit, presence of periphyton etc.) combined with adaptive stress. When the poor conditions lead to an immunity loss of the fish and the disease sets in. Purpose of this paper is to find new treatments that act simultaneous internally on the pathogen inside the trout through administration of antibiotics in food, but also externally by destroying the residual bacteria found in mud and periphyton, through administration of non-toxic disinfectants, which does not cause side effects to the antibiotic medication. The work method consists in applying the same antibiotic treatment in the two ponds (B2, B3) under identical growth conditions and density, selected from the same batch of trout yersiniosis, with the difference that in the B3 pond, oral administration of medication was cumulated with long term peracetic acid baths. The diagnosis of Yersinosis was based on the identification of the first symptoms of the disease and was confirmed by microbiological examination and the treatment was based by sensitivity tests which decided the type of administered antibiotic. Rresults were quantified by monitoring mortalities in the two ponds and shows that in the B3 pond where the trout was treated with antibiotic and disinfectant, the recorded mortality is 3.18% lower. The current experiment, using an antibiotic treatment administered orally, combined with a general disinfectant, administered in the water, peracetic acid, reduced the rainbow trouts mortality caused by Yersiniosis. [ABSTRACT FROM AUTHOR]

Lactococcus garvieae is the aetiological agent of lactococcosis, a haemorrhagic septicaemia that affects marine and freshwater fish, with special incidence and economic relevance in farmed rainbow trout. Water temperature is one of the most important predisposing factors in the development of lactococcosis outbreaks. Lactococcosis in trout usually occur when water temperatures rise to about 18 °C, while fish carriers remain asymptomatic at temperatures below 13 °C. The aim of this work was to analyse the differences in the complete transcriptome response of L. garvieae grown at 18 °C and at 13 °C and to identify the immunogenic proteins expressed by this bacterium at 18 °C. Our results show that water temperature influences the expression of L. garvieae genes involved in the lysis of part of the bacterial cell population and in the cold response bacterial adaptation. Moreover, the surface immunogenic protein profile at 18 °C suggests an important role of the lysozyme-like enzyme, WxL surface proteins and some putative moonlighting proteins (proteins with more than one function, usually associated with different cellular locations) as virulence factors in L. garvieae. The results of this study could provide insights into the understanding of the virulence mechanisms of L. garvieae in fish. [ABSTRACT FROM AUTHOR]

Simple Summary: Viruses are pervasive components of aquatic ecosystems, and most of them are harmless to humans and animals; however, several aquatic viruses can infect animals, leading to diseases, especially when fish are confined, such as in aquaculture facilities. Traditional methods used to detect and study viruses have been widely applied to aquatic animals' viruses, leading to the successful isolation, identification and understanding of several of them. However, they have limits, which can be overcome by molecular methods, such as polymerase chain reaction (PCR)-based assays, sequencing and in situ hybridisation. A standard PCR, followed by the sequencing of purified amplicons, is an effective method for both identifying well-known viruses and discovering new ones. In situ hybridisation, in which a labelled probe binds to a nucleic acid sequence in tissue, is able to correlate the presence of viruses to lesions. Novel molecular isothermal methods, such as loop-mediated isothermal amplification (LAMP), were also developed and applied to viral aquatic animal diseases, bringing molecular diagnosis into the field. This review considers the scientific literature dealing with the molecular methods employed hitherto to study the most relevant finfish and shellfish viral pathogens, stressing their advantages and disadvantages. Aquaculture is the fastest-growing food-producing sector, with a global production of 122.6 million tonnes in 2020. Nonetheless, aquatic animal production can be hampered by the occurrence of viral diseases. Furthermore, intensive farming conditions and an increasing number of reared fish species have boosted the number of aquatic animals' pathogens that researchers have to deal with, requiring the quick development of new detection and study methods for novel unknown pathogens. In this respect, the molecular tools have significantly contributed to investigating thoroughly the structural constituents of fish viruses and providing efficient detection methods. For instance, next-generation sequencing has been crucial in reassignment to the correct taxonomic family, the sturgeon nucleo-cytoplasmic large DNA viruses, a group of viruses historically known, but mistakenly considered as iridoviruses. Further methods such as in situ hybridisation allowed objectifying the role played by the pathogen in the determinism of disease, as the cyprinid herpesvirus 2, ostreid herpesvirus 1 and betanodaviruses. Often, a combination of molecular techniques is crucial to understanding the viral role, especially when the virus is detected in a new aquatic animal species. With this paper, the authors would critically revise the scientific literature, dealing with the molecular techniques employed hitherto to study the most relevant finfish and shellfish viral pathogens. [ABSTRACT FROM AUTHOR]

For the maritime sector to be sustainable and to have an intact blue economy, shipowners should be ready to implement Ship Energy Efficiency Management Plans alongside energy efficiency projects. The problem for organizations and shipowners having fishing carriers older than 20 years is highlighted and the following challenges arise for decision-making authorities. To keep such ships in the fleet for the next decade, shipowners should deploy energy efficiency projects for marine system retrofitting to improve energy efficiency and meet environmental regulations. An energy audit is performed and an energy efficiency program is proposed with guidelines for regulations that are currently coming into force. To improve energy efficiency, reduce the environmental impact, and cut fuel consumption costs, marine system retrofitting is done, in a particular case, with two options proposed. The first is a cascade refrigeration system with hydrocarbons and carbon dioxide, where the shipowner gains an energy efficiency improvement of about 20%. The second option is a two-stage refrigeration system with ammonium as the environmentally friendly refrigerant, which improves the energy efficiency by about 26%. Technical and economic issues have been discussed. [ABSTRACT FROM AUTHOR]

The study explored the legislative database for the maritime sector to be sustainable and have an intact blue economy. The problem for organizations with shipowners that have fishing boats older than 20 years is stated. Decision-making authorities face challenges nowadays. To be in the fleet for the next decade, shipowners for their ships should deploy energy efficiency projects for marine system retrofitting to improve energy efficiency and meet environmental regulations. The Ship Energy Efficiency Management Plan's development principles are discussed due to the current need for its deployment from 2021, so shipowners can contribute to the fast adoption of new regulations by the International Maritime Organization. In this work it is being offered to incorporate energy management issues with guidelines for upcoming regulations as well as those that are currently enforced. To improve energy efficiency, reduce environmental impact and cut fuel consumption costs, marine system retrofitting has been proposed. In this paper the focus is being put on studying energy management strengths and weaknesses which can lead to a qualitative understanding of the performance of the organizational structure, this work also focuses on the management settings concerning energy efficiency. If the organization wants to win by integrating energy policy, high-quality communication or promotion of energy efficiency issues should be employed. Marine plastic debris challenges fishing carriers on the African Union route. A possible beneficial solution for shipowners is discussed in this study. [ABSTRACT FROM AUTHOR]

Background: Janus kinase 3 (JAK3) acts downstream of the interleukin-2 (IL-2) receptor family to play a pivotal role in the regulation of lymphoid cell development. Activating JAK3 mutations are associated with a number of lymphoid and other malignancies, with mutations within the regulatory pseudokinase domain common. Methods: The pseudokinase domain mutations A572V and A573V were separately introduced into the highly conserved zebrafish Jak3 and transiently expressed in cell lines and zebrafish embryos to examine their activity and impact on early T cells. Genome editing was subsequently used to introduce the A573V mutation into the zebrafish genome to study the effects of JAK3 activation on lymphoid cells in a physiologically relevant context throughout the life-course. Results: Zebrafish Jak3 A573V produced the strongest activation of downstream STAT5 in vitro and elicited a significant increase in T cells in zebrafish embryos. Zebrafish carrying just a single copy of the Jak3 A573V allele displayed elevated embryonic T cells, which continued into adulthood. Hematopoietic precursors and NK cells were also increased, but not B cells. The lymphoproliferative effects of Jak3 A573V in embryos was shown to be dependent on zebrafish IL-2Rγc, JAK1 and STAT5B equivalents, and could be suppressed with the JAK3 inhibitor Tofacitinib. Conclusions: This study demonstrates that a single JAK3 A573V allele expressed from the endogenous locus was able to enhance lymphopoiesis throughout the life-course, which was mediated via an IL-2Rγc/JAK1/JAK3/STAT5 signaling pathway and was sensitive to Tofacitinib. This extends our understanding of oncogenic JAK3 mutations and creates a novel model to underpin further translational investigations. [ABSTRACT FROM AUTHOR]

Grouper aquaculture is rapidly expanding in both tropical and subtropical regions. The presence of marine leeches (Pterobdella arugamensis; previously named Zeylanicobdella arugamensis) infesting cultured groupers, however, can have a fatal effect on grouper aquaculture production and cause significant economic loss. Understanding the marine leech's population structure is therefore important to determine its possible distributional origin and distributional mechanisms, which will help monitor and mitigate the infestation. In this study, a total of 84 marine leeches collected from cultured hybrid groupers Epinephelus spp. in Brunei Darussalam, Malaysia and Indonesia were identified as P. arugamensis, based on morphological and mitochondrial cytochrome c oxidase subunit I gene sequence analyses. These leech samples, together with additional sequences from the GenBank database, were grouped into four genetically distinct haplogroups: (1) Asia Pacific, (2) Borneo, (3) Surabaya and (4) Iran. The four populations were found to be highly diverged from each other. The results also suggested that the samples from the Asia Pacific population could be dispersed and transported from Indonesia. [ABSTRACT FROM AUTHOR]

This study aimed to explore the marketing channel, marketing cost and marketing margin and problems associated with fish marketing in order to provide recommendations for efficient fish marketing system in Barisal city, southern Bangladesh. Data were collected from seven fish markets of Barisal, southern Bangladesh including Port road fish market, Taltoli bazar fish market, Chaumatha fish market, Notullabad bus stand fish market, Kasipur bazar fish market and Bazar road fish market. Both primary and secondary data were used during 1 year study period from May, 2011 to April, 2012. Primary data were collected through field surveys and pre-structured questionnaire was used for interviewing of 10 organizers, 20 aratdars (commission agent), 50 fish traders and 100 consumers. Secondary source of information consists of published materials such as journals, textbooks, newspapers and also from interviewing different government and non-government officials and members of various fisheries related cooperative societies. The results of this study revealed 11 fish marketing channels in Barisal city's fish marketing system. The average marketing cost ranged from 4.15% to 8.33% (6.76±0.98) % of the final retail price. The highest and lowest average fish price were recorded as USD 7.07/kg and USD 1.03/kg for giant fresh water prawn and silver carp, correspondingly. Intermediaries' marketing margin on different species' marketing fluctuated from 29.6% to 50.28% (39.38±5.22) % for silver carp and brown shrimp marketing, respectively. Establishment of modern fish landing center and retail markets near the fish landing port, introduction of government fish shops and insulated and refrigerated fish vans and fish carriers, training of all personnel related to fish marketing about fish handling, quality of fish, hygiene practices, improvement of existing fish market structure, provision for government and private funding assistance for fishers/fish farmer, formulation and enactment of independent act/ordinance for fish landing and marketing are highly recommended. [ABSTRACT FROM AUTHOR]

We propose the concept of Accelerated Marine Extraction to signify two periods when rapidly increasing cod (Gadus morhua, Gadidae) and herring (Clupea harengus, Clupeidae) fisheries, c.1540–1600 and c. 1730–1790, exceeded human demographic growth. Total landings vastly exceeded previous assessments and more than doubled between 1520 and 1620 from about 220,000 metric tonnes (t) to 460,000 t. Supplies of cod and herring to the European market peaked in 1788 at more than 1 million t before the unrest connected with the French Revolution brought many fisheries to a temporary halt. Accelerated Marine Extractions increased European food security at times of human demographic growth by almost doubling the supplies of fish protein per capita. While herring was the most important species by 1520, cod dominated through the period 1540–1790, and the trajectories of cod and herring extractions differed significantly. Cod landings increased almost ten‐fold between 1520 and 1790, driven by strong and sustained landings in the Northwest Atlantic. Herring landings remained stable through the 16th century but declined severely through the next 150 years. However, from 1750, herring landings quadrupled, largely because of Swedish west coast fisheries. The results fundamentally shift our understanding of the scale of Atlantic fisheries in the past and underline the role of marine resources for European societies. [ABSTRACT FROM AUTHOR]

Farmed marine fish constitute 20% of the total farmed fish production in Egypt, and the Deeba Triangle produces a relatively large portion of this percentage. Last year, several private fish farms in the Deeba Triangle have suffered severe economic losses due to acute fish mass kills. This study aimed to investigate the hidden aetiologies behind these colossal mass fish kills and to propose an emergency control strategy. Several tons of dead farmed fish were remarkably scattered throughout affected ponds and at the vicinity of impacted fish farms. Moribund farmed European seabass (Dicentrarchus labrax), thin‐lipped grey mullet (Liza ramada) and gilthead seabream (Sparus aurata) have exhibited skin darkness, emaciation, congested gills and fins, ascites, skin erosions and ulcerations. Internally, moribund fish emitted unpleasant odour upon opening the abdomen together with severe congestion and haemorrhages in kidneys and brain. Mottled atrophied spleens were the most prominent findings, while the gastrointestinal tracts were filled with whitish caseous material. The liver was pale with multiple whitish nodules. Photobacterium damselae was the most retrievable bacterial pathogen from most infected fish and trash fish. Photobacterium damselae subspecies piscicida and Photobacterium damselae subspecies damselae were definitively identified from examined moribund fish using both conventional morpho‐chemical and molecular assays. Data analysis has revealed that the poor water quality was profoundly incriminated in triggering the bacterial infections with a fate of mass mortalities. Conclusively, adopting various strict biosecurity strategies will be the key factors in prevention of future episodes of mass kills. [ABSTRACT FROM AUTHOR]

Combating and controlling sea lice causes large economic costs for the farmers, with estimated values of more than 305 million euros (€) per year. Increased resistance against traditional chemotherapeutants due to evolutionary drivers in the sea lice combined with the lack of an effective vaccine and few other chemical treatments available are expected to cause these costs to increase. Several possible methods for managing sea lice infestations have been investigated, but only cleaner fish has proven to have an effect on lice levels. Cleaning activity is well known in marine fish and has been observed in the wild as a form of symbiosis between two species: one species, the 'client' fish, seek out the other species, the 'cleaner' fish, to have ectoparasites and dead tissue cleared from its body. The Atlantic lumpfish is a relatively new aquaculture species, and wild‐caught mature fish are used as brood stock for farmed production. This poses a biosecurity risk, as wild fish can carry pathogens, and the use of quarantine and health screening is recommended. Vaccine development is unfortunately lagging far behind relatively to the wide spread and high utilisation of the fish. This review contains description of the main pathogens and diseases that affect cleaner fish. [ABSTRACT FROM AUTHOR]

This article examines livelihood strategies of fishers and youth in an urban fishing community in India. Situated next to the busiest fishing harbour in Karnataka, I show how proximity to the city provides fishers and youth broader occupational choices to diversify their livelihoods by intensifying or taking on several fisheries-based activities, moving into the service sector, or getting urban jobs. Urban conditions have largely influenced how fishers and youth decide their livelihood strategy. The article shows how the fishers and youth have employed livelihood diversification via both accumulation and risk management strategies. Due to the lack of analysis drawing on urban fisheries case studies, the narratives of small-scale fisheries have largely been based on rural contexts, which often portrait small-scale fishers as either inefficient or vulnerable. This study, however, allows us to open up existing small-scale fisheries narratives to view fishers as active agents. Therefore, this study calls for more systematic emphasis on studying urban implications in small-scale fishing communities with important repercussions for urban fishers and their livelihood strategies. [ABSTRACT FROM AUTHOR]

Gata2 is a key transcription factor required to generate Haematopoietic Stem and Progenitor Cells (HSPCs) from haemogenic endothelium (HE); misexpression of Gata2 leads to haematopoietic disorders. Here we deleted a conserved enhancer (i4 enhancer) driving pan-endothelial expression of the zebrafish gata2a and showed that Gata2a is required for HE programming by regulating expression of runx1 and of the second Gata2 orthologue, gata2b. By 5 days, homozygous gata2aΔi4/Δi4 larvae showed normal numbers of HSPCs, a recovery mediated by Notch signalling driving gata2b and runx1 expression in HE. However, gata2aΔi4/Δi4 adults showed oedema, susceptibility to infections and marrow hypo-cellularity, consistent with bone marrow failure found in GATA2 deficiency syndromes. Thus, gata2a expression driven by the i4 enhancer is required for correct HE programming in embryos and maintenance of steady-state haematopoietic stem cell output in the adult. These enhancer mutants will be useful in exploring further the pathophysiology of GATA2-related deficiencies in vivo. Dobrzycki et al delete a conserved enhancer that drives endothelial expression of the transcription factor Gata2a in Zebrafish and find that it is required for programming of haemogenic endothelium and for maintenance of haematopoietic stem cells in the adult. These data may help understanding Gata2 deficiency syndromes in humans. [ABSTRACT FROM AUTHOR]

Abundance and antibiotic resistance of bacteria of the genus Aeromonas isolated from the water of three carp ponds were studied. The number of those bacteria differed between the studied ponds, sites and season. The results of the present study showed that planktonic Aeromonas inhabiting those ponds strongly differed in the resistance level to tested antibiotics. These microorganisms were the most resistant to amoxicillin, ampicillin, clindamycin and penicillin. However, all isolates Aeromonas were susceptible to gentamycin and streptomycin. Majority of bacterial strains were characterized by resistance to 4–6 of the 12 antibiotics tested. Bacterial resistance to antibiotics depended on their chemical structure. Aeromonas strains isolated from the studied ponds were the most resistant to β-lactam and lincosamides antibiotics, while the most susceptible to aminoglycosides, chloramphenicols and fluoroquinolones. [ABSTRACT FROM AUTHOR]

Indonesia is one of the largest exporters of groupers, both live and dead, as part of the reef fish food trade. These fisheries sub-sector plays an important role in the livelihoods of fishing communities, especially for those living on small islands. The aim of this article is to provide a baseline profile of the trade as currently practiced and develop a framework strategy for establishing a sustainable grouper export trade for Indonesia. Challenges faced in managing the country's (both live and dead) grouper fishery and trade include the overfishing of groupers, catch of sexually immature groupers, exploitation of grouper spawning aggregation, illegal and unreported fishing for live groupers and overall lack of management or monitoring of grouper fisheries. These factors, collectively, have resulted in declining wild grouper stocks. Management and export trade of these fisheries requires regular and standardized monitoring, management and trade controls. Control on minimum size, fishing quotas and improved spawning aggregation protection to ensure sustainability are needed in addition to oversight of vessel activity to control the trade, especially exports. To maintain viable stocks, it is essential to safeguard adequate spawning capacity and reduce the illegal fishing methods (particularly potassium cyanide and compressor diving). In particular, it is important to ensure that sufficient fish become sexually mature and that sufficient adults are able to breed, particularly those that depend on aggregating to spawn because they are easy to over-fish in such time, requiring their seasonal and/or spatial protection from fishing. Since the grouper fisheries are particularly heavily driven by export markets, regulating volumes and sizes exported, especially oversight of at-sea and air cargo exports is needed. Immediate measures are essential to stop further declines and begin to rebuild grouper stocks in Indonesia. [ABSTRACT FROM AUTHOR]

Humphead, or Napoleon, wrasse, (Cheilinus undulatus), is a large reef fish naturally vulnerable to over‐exploitation and highly prized as luxury seafood. It is predominantly consumed in Hong Kong and mainland China. Hong Kong is the global trade hub for this species. In 2004 (effective 2005), the humphead wrasse was listed on the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Appendix II that substantially improved understanding and management of the species although illegal, unregulated and/or undocumented trade (IUU) continues.To understand patterns of trade in humphead wrasse, evaluate implementation of the CITES listing and identify ways to tackle IUU, especially in Hong Kong, data from multiple sources, including government databases between 1999 and 2017 and independent retail surveys between 2014 and 2017, were compiled.IUU trade into and through Hong Kong was detected, but declined following increased government enforcement in 2016. Nonetheless, fish numbers on sale continue to exceed imports with CITES permits and mainland China only sparsely reports imports of the species. Approximately 0.5–1.5 metric tonnes were traded internationally annually.Several legislative loopholes undermine enforcement into and within Hong Kong, including poor oversight of live fish carrier vessels and air imports, excessively long validity period for Licences to Possess in the city, non‐compliant traders, and legislative exemptions or omissions. The introduction of a ranched fish category in 2018 by Indonesia created additional challenges. More intelligence and risk analyses could help enforcers target their work.A major enforcement challenge is the inability to distinguish legally from illegally imported fish within Hong Kong. To improve fish tracking, identifying individuals using their complex facial patterns as fingerprints was explored; proof‐of‐concept was demonstrated using a mobile phone application: Saving Face. Facial marks were determined to be long‐lasting and different enough to distinguish among multiple individuals. [ABSTRACT FROM AUTHOR]

On-board live storage of Atlantic cod caught by trawl was evaluated as a potential method to improve the color characteristics of fillets. Before slaughter and processing, the fish were: (i) stored live for 3–6 h, (ii) kept in dry bin, or (iii) stored on deck for 5 h post capture (without bleeding). Blood chemistry and white muscle biochemistry were determined after capture and live storage. Fillet color and presence of discolorations were assessed on board (fresh) and after frozen storage (market quality). All fish were considerably stressed by capture and did not recover to baseline levels after live storage. Processing just after capture, live or dry bin storage, resulted in fillets with good color characteristics. Delayed processing (5 h) resulted in fillets with inferior color characteristics. The color characteristics of fillets evaluated on board and after frozen storage followed a similar pattern, although fillets from all treatments exhibited a more yellowish tint after frozen storage. Under the present catch and processing conditions, live storage on board did not improve the color characteristics of cod fillets. Color analysis indicated that consumers would only be able to identify worst-case fillets cut from unbled fish as being inferior to fillets from all other treatments. [ABSTRACT FROM AUTHOR]

Microbial communities are complex and dynamic, composed of hundreds of taxa interacting across multiple spatial scales. Advances in sequencing and imaging technology have led to great strides in understanding both the composition and the spatial organization of these complex communities. In the human mouth, sequencing results indicate that distinct sites host microbial communities that not only are distinguishable but to a meaningful degree are composed of entirely different microbes. Imaging suggests that the spatial organization of these communities is also distinct. Together, the literature supports the idea that most oral microbes are site specialists. A clear understanding of microbiota structure at different sites in the mouth enables mechanistic studies, informs the generation of hypotheses, and strengthens the position of oral microbiology as a model system for microbial ecology in general. [ABSTRACT FROM AUTHOR]

The high volume of traffic originates two well-known problems in many cities: congestion and pollution. In recent years, a social phenomenon is emerging cooperation. This work is aimed at evaluating the circumstances under which transport cooperation is possible between different stakeholders operating in the same geographical area. To this end, a double survey process was conducted in a marketplace situated in the Seville City (Spain) centre. The first survey was designed to know the characteristics of the retailers and their preferences with respect to cooperation and regulations. A relational analysis between retailer features and their willingness to cooperate was carried out. After analysing the motivations for non-cooperation, a mixed proposal was designed and surveyed. Although the research was limited to a marketplace, the relevant data gathered from this double survey process highlights some implications: (a) the importance of personal relations in retailer cooperation; (b) a high volume of freight and the use of vans as on-street warehouses appear as significant motivations for non-cooperation; (c) forcing changes in the statu quo encourages cooperation. [ABSTRACT FROM AUTHOR]

Electronic monitoring (EM) consisting of on-board video imagery and on-shore analysis, offers an alternative or supplement to at-sea observer programs in commercial fisheries. In the western and central Pacific Ocean (WCPO), where observer coverage in most tuna longline fisheries has historically been < 5%, the advent of EM has been perceived as a tool for meeting international data collection and exchange obligations. However, the capability of EM to collect and support interpretation of records into data for all fields currently collected by at-sea observers is still under assessment. We use the Western and Central Pacific Fisheries Commission (WCPFC) as a case-study to evaluate the longline WCPFC regional observer programme minimum standard data fields, their current scientific application, the proportion of member countries exchanging data and the capability of EM technology to collect these fields. We identify that 78% of the longline fields can be collected with current EM technology, with 84% of these used in scientific analyses. For the 16% of fields not routinely used in scientific analyses, the introduction of EM may facilitate a sufficient increase in data availability to support their future use. Alternative tools would be required to collect fields that EM could not record to ensure data continuity and scientific rigour are not compromised. In examining the capability of EM in the context of WCPFC member state requirements under international law, we advocate for a holistic and integrated approach to the use of EM in future research and monitoring programs in both the WCPO and global longline fisheries. [ABSTRACT FROM AUTHOR]

The article discusses research on legal issues concerning the registration and proprietary status of fishing boats in Lamongan, Indonesia. Topics explored include the fishing boat registration rules at Pelabuhan Perikanan Nusantara Brondong in Lamongan, the fishing vessel classification based on the Indonesia Ship Nationality Certificate system, and the boat registration requirements such as fishery business license and ship measurement document.

Previous studies from the coastal cargo sector report of a considerable gap between formal and informal aspects of safety. Several maritime accident investigations point to this gap as an important contributory cause, indicating that risk increases when informal practices ignore or violate formal procedures. The main aims of this paper are therefore to examine the factors influencing procedure negligence in the coastal cargo sector, and to discuss how to reduce the gap between formal and informal aspects of safety. The study is based on quantitative survey results of 180 crewmembers and qualitative research interviews with 10 leading officers on cargo vessels sailing along the coast of Norway. Survey results indicate that procedure negligence is related to work pressure and demanding working conditions. It is also related to outcome measures like personal injuries and perceptions of risk and safety. A positive organizational safety culture is experienced by respondents reporting of less procedure negligence. Interview results indicate, for example, that formal procedures are ignored or violated because of issues like efficiency demands and low-quality procedures, and that procedures more often are followed when they are perceived as useful and timely. In this article, it is thus suggested that a positive safety culture includes an alignment of formal and informal aspects of safety, which requires time to comply with practical procedures. Interviewees suggest that appropriate procedures are: (1) developed by the users, (2) continuously improved, (3) simplified, and (4) supervised by local expertise instead of general experts. [ABSTRACT FROM AUTHOR]

Cooperation by Pacific Island countries in the management of tuna fisheries is an important means of achieving sustainable development of these shared resources, in line with Sustainable Development Goal 14. The Western and Central Pacific Ocean (WCPO) tuna fishery is the largest tuna fishery in the world and two examples of arrangements based on regional cooperation are provided: The purse seine Vessel Day Scheme (VDS) is a fisheries management system that is being implemented through the cooperation of the Parties to the Nauru Agreement (PNA), which regulates harvesting of tuna in line with SDG 14.4. The scheme also establishes rights in the shared fishery for small island developing states, increasing economic returns in support of SDG 14.7. Regional cooperation in fisheries Monitoring Control and Surveillance is a unique collaboration between the members of the Forum Fisheries Agency (FFA) to address illegal, unreported and unregistered (IUU) fishing in support of SDG 14.4. A range of regionally agreed systems and tools is applied. [ABSTRACT FROM AUTHOR]

This paper presents the energy and greenhouse gas (GHG) emission assessments of Korean offshore fisheries. The consumption of energy by fisheries is a significant concern because of its attendant environmental effect, as well as the cost of the fuel consumed in fishing industry. With the global attention of reducing GHG emission and increasing energy efficiency of fuel, the seafood industry needs to further understand its energy use and reduce its GHG emission. In the present study, the amount of energy consumed and the GHG emission of Korean offshore fisheries in a period from 2009 to 2013 were examined. Offshore fisheries accounted for 24% of Korean production in 2013 and 60% of fuel consumption related GHG emission. Whereas the total GHG emission intensity of this sector improved slightly between 2009 and 2012; as such emission decreased by approximately 1.9%, which increased again in 2013. The average amount of total GHG emission in this five years period was 1.78 × 106 tons of carbon dioxide equivalent/year (t CO2 eq. y−1). Active fishing gear was found to consume 20% more fuel than passive gear. However, the production from passive gear was 28%, lower than 72% from active gear. The reason for this is that less abundant stationary resources are harvested using passive gear. Furthermore, the consumption of fuel was significantly influenced by the fishing method. Implementation and development of new fishing technologies and methods are important for improving energy efficiency and reducing the climate impact on fisheries. To realize these purposes, the fishery management system needs to be established by centralizing on energy efficiency and climate effect. [ABSTRACT FROM AUTHOR]

Cyprinid herpesvirus 3 (CyHV-3), also known as koi herpesvirus, is the causative agent of the highly contagious koi herpesvirus disease, which is restricted to koi and common carp and causes significant losses in both fish stock. Some experimental investigations have shown that other cyprinid or non-cyprinid species may be asymptomatically susceptible to this virus and might play roles as potential carriers of CyHV-3 or might contribute to persistence of this virus in environment. Therefore, it seems important to verify not only the susceptibility of other cyprinid or non-cyprinid species, but also their ability to transmit CyHV-3 infection to susceptible species. Our previous investigation of the susceptibility of the topmouth gudgeon (Pseudorasbora parva) did not reveal the presence of CyHV-3 DNA in the tissues of this species after cohabitation with infected koi. Consequently, we changed the experimental conditions and applied two stress factors (removal of skin mucus and scaring) which would presumably mimic the stress most commonly encountered in the wild. Both experiments (without and with stress factors) consisted of primary and secondary challenges. In both the no-stress and stress experiments, the first challenge was focused only on testing the susceptibility of the topmouth gudgeon to the virus. With the secondary challenge, we investigated potential viral transmission from the topmouth gudgeon to healthy naive koi after exposure to stress factors. All fish (dead, surviving and sacrificed) were tested for the presence of CyHV-3 DNA using nested PCR (no-stress experiment) and real-time PCR (stress experiment). After the primary challenge of the no-stress experiment, PCR did not reveal the presence of CyHV-3 DNA in any specimen of cohabitated topmouth gudgeon, but all specimens of dead koi were CyHV-3 DNA-positive. PCR of fish tissues subjected to the secondary challenge did not show the transfer of virus to naive fish. After exposure to stress (removal of skin mucus), qPCR revealed four out of five samples (80%) of topmouth gudgeon to be positive for CyHV-3 DNA. Two out of five samples (40%) of topmouth gudgeon treated by scaring were found to be positive for the presence of viral DNA. Real-time PCR after the secondary challenge did not reveal any viral DNA positivity in specimens of topmouth gudgeon from groups previously exposed to stress. The stress experiments show that removal of skin mucus might potentially lead to susceptibility of topmouth gudgeon to CyHV-3 infection, but the transmission of the virus to koi carp was not observed. [ABSTRACT FROM AUTHOR]

Distant hybridization refers to crosses between two different species, genera, or higher-ranking taxa, which can break species limits, increase genetic variation, and combine the biological characteristics of existing species. It is an important way of creating genetic variation, fertile strains, and excellent characteristics in new strains and populations. Combining analyses and summaries from many inter-related documents in plants and animals, both domestic and international, including examples and long-standing research on distant hybridization in fish from our laboratory, we summarize and compare the similarities and differences in plant and animal distant hybridization. In addition, we analyze and review the biological characteristics of their different ploidy progenies and the possible causes of disparity in survival rates. Mechanisms of sterility in animal and plant distant hybrids are also discussed, and research methods for the study of biological characteristics of hybrids, including morphology, cytology, and molecular cytogenetics are presented. This paper aims to provide comprehensive research materials and to systematically compare the general and specific characteristics of plant and animal hybrids with regards to reproduction, genetics, growth traits, and other biological characteristics. It is hoped that this paper will have great theoretical and practical significance for the study of genetic breeding and biological evolution of plant and animal distant hybridization. [ABSTRACT FROM AUTHOR]

For the past several decades, Norwegian shipyards have focused on innovative, customized, and technologically advanced ships, often serving the offshore oil and gas industry. Usually, a significant part of the ship production is offshored, especially steel-related tasks. That is, such tasks are carried out in a country with lower factor costs. The Norwegian yards focus on the more advanced outfitting tasks, such as the installation and commissioning of machinery and deck equipment, electrical systems, and accommodation. Nevertheless, the amount of work performed abroad before the Norwegian yard takes over and continues production differs among various yards; some only offshore block construction, others construction of the entire hull. Yet others finish the ship to such a degree abroad that it does not need to be recovered from the water in Norway and all the remaining work can be done from the quayside. This paper introduces a typology of shipbuilding strategies that differ in how much of the steel and outfitting work is performed in a country with lower cost levels. The strategies are discussed and compared in terms of relevant build strategic elements, such as preoutfitting, concurrent execution of engineering and production, yard capabilities, and vertical integration. The strategies' likely effect on performance is also addressed, in terms of costs, quality, delivery dependability, delivery time, and flexibility. The results are based on a qualitative study of Norwegian yards and their offshoring strategies. [ABSTRACT FROM AUTHOR]

Background: Dorsal spine reduction in threespine sticklebacks (Gasterosteus aculeatus) is a classic example of recurrent skeletal evolution in nature. Sticklebacks in marine environments typically have long spines that form part of their skeletal armor. Many derived freshwater populations have evolved shorter spines. Changes in spine length are controlled in part by a quantitative trait locus (QTL) previously mapped to chromosome 4, but the causative gene and mutations underlying the repeated evolution of this interesting skeletal trait have not been identified. Results: Refined mapping of the spine length QTL shows that it lies near the MSX2A transcription factor gene. MSX2A is expressed in developing spines. In F1 marine x freshwater fish, the marine allele is preferentially expressed. Differences in expression can be attributed to splicing regulation. Due to the use of an alternative 5' splice site within the first exon, the freshwater allele produces greater amounts of a shortened, non-functional transcript and makes less of the full-length transcript. Sequence changes in the MSX2A region are shared by many freshwater fish, suggesting that repeated evolution occurs by reuse of a spine-reduction variant. To demonstrate the effect of full-length MSX2A on spine length, we produced transgenic freshwater fish expressing a copy of marine MSX2A. The spines of the transgenic fish were significantly longer on average than those of their non-transgenic siblings, partially reversing the reduced spine lengths that have evolved in freshwater populations. Conclusions: MSX2A is a major gene underlying dorsal spine reduction in freshwater sticklebacks. The gene is linked to a separate gene controlling bony plate loss, helping explain the concerted effects of chromosome 4 on multiple armor-reduction traits. The nature of the molecular changes provides an interesting example of morphological evolution occurring not through a simple amino acid change, nor through a change only in gene expression levels, but through a change in the ratio of splice products encoding both normal and truncated proteins. [ABSTRACT FROM AUTHOR]

Infectious pancreatic necrosis virus (IPNV) has been isolated annually since 1987 from salmonids without clinical signs at coastal fish farms in Finland. In the inland area, viral isolations were rare until 2012, when IPNV was detected at several freshwater fish farms. Between 2013 and 2015, the infection spread and IPNV was continuously isolated from several farms, both inland and on the coast. The aim of this study was to genetically characterise the IPNV isolates collected from Finnish coastal and inland fish farms over the last 15 years, and to detect genetic changes that may have occurred in the virus populations during the study period. The partial VP2 gene sequence from 88 isolates was analysed. In addition, a complete genomic coding sequence was obtained from 11 isolates. Based on the genetic analyses, Finnish IPNV isolates belong to three genogroups: 2, 5 and 6. The genetic properties of the isolates appear to vary between inland farms producing juveniles and food fish farms in the coastal region: the inland farms harboured genogroup 2 isolates, whereas at coastal farms, all three genogroups were detected. Little genetic variation was observed within the Finnish genogroup 2 and 5 isolates, whereas among the genogroup 6 isolates, two subgroups were detected. All isolates studied demonstrated amino acid patterns in the viral VP2 gene previously associated with avirulence. However, increased mortality was detected at some of the farms, indicating that more research is needed to clarify the relationship between the pathogenicity and genetic properties of IPNV isolates from different genogroups. [ABSTRACT FROM AUTHOR]

Triggered by urbanization and changing land use, coastal transformation is a rapidly increasing phenomenon in the global south, driving dramatic livelihoods impacts. However, the existing literature on small-scale fisheries (SSF) has paid little attention to the way coastal transformations shape conditions for SSF livelihoods communities. This study proposes a new orientation in SSF studies by exploring the assemblage of entangled sociomaterial processes that account for coastal transformations by investigating waterfront transformation in a fishing community in Karnataka, India. Drawing on ethnographic fieldwork, we conclude that an entanglement of sociomaterial processes produces unequal outcomes among stakeholders that subsequently reinforce the political and economic marginalization of certain groups of waterfront users. Moreover, the investigated context-specific waterfront assemblage intimately connects to the broader context of national fishery policy, urbanization, and tourism, directing the way coastal space can and should be transformed. Such an analysis contributes to the understanding of changing livelihoods in SSF communities. [ABSTRACT FROM AUTHOR]

Viral encephalopathy and retinopathy ( VER), otherwise known as viral nervous necrosis ( VNN), is a major devastating threat for aquatic animals. Betanodaviruses have been isolated in at least 70 aquatic animal species in marine and in freshwater environments throughout the world, with the notable exception of South America. In this review, the main features of betanodavirus, including its diversity, its distribution and its transmission modes in fish, are firstly presented. Then, the existing diagnosis and detection methods, as well as the different control procedures of this disease, are reviewed. Finally, the potential of selective breeding, including both conventional and genomic selection, as an opportunity to obtain resistant commercial populations, is examined. [ABSTRACT FROM AUTHOR]