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Antilymphocytic globulin (ALG) depressed the antibody responses of mice to SRBC as measured in terms of splenic PFCs of IgM, IgG1, IgG2a and IgG2b specificity. This effect was apparent over a range of antigen does. ALG also reduced the antibody responses of congenitally athymic mice to SRBC and SHI. The possible mode of action of ALG is discussed in the light of these, and other, observations. [ABSTRACT FROM AUTHOR]

ALG has been isolated from the sera of four horses immunized with mouse thymocytes and its ability to suppress the immune response to SSS-III investigated. All four preparations suppressed the response to this antigen in BALB/c mice but only one was effective in CBA mice. Other ALGs were isolated from sequential bleeds obtained from a single horse. Preparations obtained after only two injections of the donor with mouse thymocytes, were able to suppress the immune response to SSS-III in CBA and BALB/c mice. Studies with selected ALG preparations indicated that they were less effective or ineffective if their administration was delayed until just before challenge with SSS-III. These results indicate that the ability of ALG to suppress thymus-independent immune processes is a variable one, but it is not necessarily a consequence of hyperimmunization of the donor animal. It is however influenced by the ALG protocol used and the strain of animal under test. [ABSTRACT FROM AUTHOR]

The effect of an horse anti-mouse thymocyte globulin (ALG) preparation on the primary and secondary immune responses to sheep erythrocytes (RBC) was studied in 2-3-month-old male A/HeJ, C57B1, BALB/c, DBA/1, CBA and C3H mice. A wide variation in the primary and secondary responses of the individual strains was noticed but in ail cases these were significantly suppressed by ALG pretreatment. The capacity of the ALG to suppress the primary immune response was not overcome by increasing the antigen dose from 3 × 107 to 3 × 109 sheep RBC. [ABSTRACT FROM AUTHOR]

When 14C-cortisone was given to pregnant A/Jax and C57BL mice, the concentration of radioactivity firmly bound to fetal tissue was significantly higher in A/Jax mice than in C57BL mice. Differences in the binding of cortisone to tissue proteins may explain the differential incidences of cleft palate in A/Jax and C57BL mice. [ABSTRACT FROM AUTHOR]