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SUMMARY The protein in wholewheat unbaked bread ingredients, microwave-baked wholewheat bread, oven-baked wholewheat bread, and two breakfast cereals was evaluated by gain in body weight of rats, protein efficiency ratio, increase in carcass nitrogen, and apparent availability of lysine as determined by fecal excretion. No significant differences were noted between the bread ingredients and microwave-baked bread. Oven-baked bread gave values significantly lower, 24–45%, than those of the ingredients. The apparent availability of lysine in bread ingredients and in microwave-baked bread was 96% and 95%, respectively, whereas that for oven-baked bread was 70%. The protein in one breakfast cereal barely maintained the weight of the animals, and gave low values for protein efficiency ratio and increase in carcass nitrogen. Seventy-five percent of the lysine was available. The other cereal allowed good growth of the animals and gave values similar to those for nonfat milk. In this oat-and-wheat-germ cereal, 88% of the lysine was available. A linear relation was obtained between either gain in body weight or increase in body nitrogen and the amount of available lysine ingested.

Interpretation of percentages obtained in phagocytic tests on patients undergoing treatment (in this instance, diathermy) is made difficult by the facts that the total leukocyte count in such patients varies and the concentration of leukocytes used in the test sensibly affects the percent of phagocytosis. To evaluate this variation it was necessary to devise a phagocytic test in which the leukocytes would be subjected to the least possible manipulation. Two cc. of heparinized salt solution (containing 1 mg. of heparin per cc. of 0.9% NaCl) are put into a test tube and about 9 cc. of blood added. In a second dry tube about 1 cc. of blood is collected and allowed to clot. The heparinized sample is centrifuged and the cells are washed once with salt solution, then divided into 2 portions. Portion A remains untreated, portion B is deleukocyted by a modification of the method of Fleming.1 A U-tube is prepared of glass tubing with a constriction in one limb into which absorbent cotton is packed tightly. The oth...

The effect of graded doses of irradiation (cobalt-60) on the morphology of McCoy cells was analyzed, and 4,000 to 5,000 r was selected as a satisfactory dose for production of giant cells. The susceptibility of radiation-induced giant cells to chlamydial infection was compared with that of nonirradiated cells by using three strains of Chlamydia trachomatis and one of C. psittaci . Monolayers of giant cells were more susceptible than normal McCoy cells as indicated by (i) greater numbers of inclusions (four- to eightfold) per unit area of monolayer, (ii) larger inclusions (fourfold greater in area), (iii) higher infective titers (1 log or more greater) of harvested cells, and (iv) greater ease of promoting a second cycle of growth. Graded doses of irradiation were applied also to mouse fibroblast (L) cells, and a similar increase in susceptibility to chlamydial infection was noted. It is concluded that giant cells produced by irradiation possess advantages over nonirradiated cells in culture for growth of Chlamydia .