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A fluorescent probe (probe 1) with selectivity and sensitivity for H 2 S and N 2 H 4 has been developed. Probe 1 exhibits dual-excitation and dual-emission fluorescence response to H 2 S and fluorescence enhancement toward N 2 H 4 with distinct naked-eye color changes. Moreover, probe 1 displayed low detection limits for H 2 S and N 2 H 4 of 0.90 nM and 23.6 nM, respectively. The reaction mechanism of probe 1 toward H 2 S and N 2 H 4 was established using 1H NMR and HR-MS spectroscopy. Additionally, probe 1 was used for monitoring and differentiating H 2 S and N 2 H 4 in HeLa cells and displays good biocompatibility and low cytotoxicity. • Probe 1 exhibits dual-emission fluorescence-enhanced detection of H 2 S in mitochondria. • Probe 1 exhibits a turn-on response for the detection of N 2 H 4 in mitochondria. • Probe 1 exhibits rapid response, good optical performance, high selectivity, and high sensitivity in solution. • Probe 1 displays multi species detection in the mitochondria of HeLa cells and zebrafish. • Detection using Probe 1 can be achieved using a smartphone with a chromaticity APP. [ABSTRACT FROM AUTHOR]

• A D-p-A type probe has been developed for detection of ClO−. • The mechanism associated with oxidation breakage of rhodanine moiety was proposed. • The crystal structure of the probe is reported. • The probe has been successfully applied to the imaging of ClO− in cells, plants and zebrafish. Hypochlorite (ClO−) plays an important role in many cellular processes, which is proved to be related to some serious diseases. Given the electron donating and electron withdrawing properties of benzo[ h ]chromene and rhodanine groups, respectively, we developed a Donor (D)-p-Acceptor (A) type probe 1 for the detection of ClO− based on benzo[ h ]chromene (D) and rhodanine (A), whose structure was determined by ESI-MS, NMR and X-ray diffraction. Probe 1 showed a fast (within 20 s), selective and sensitive response toward ClO− via a fluorescent turn-on output. Based-on the spectral change of probe 1 induced by ClO−, the mechanism associated with oxidation breakage of rhodanine moiety was proposed. Due to the excellent biocompatibility and low cytotoxicity, probe 1 can be used for imaging of ClO− in C6 cells, Arabidopsis thaliana, and zebrafish. [Display omitted] [ABSTRACT FROM AUTHOR]