1. Structural Characterization of Polygonatum Cyrtonema Polysaccharide and Its Immunomodulatory Effects on Macrophages.
- Author
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Wen R, Luo L, Zhang R, Zhou X, Wang W, and Gong L
- Subjects
- Mice, Animals, Immunologic Factors pharmacology, Immunologic Factors chemistry, Immunologic Factors isolation & purification, RAW 264.7 Cells, Cytokines metabolism, Cell Proliferation drug effects, Immunomodulating Agents pharmacology, Immunomodulating Agents chemistry, Immunomodulating Agents isolation & purification, Molecular Weight, Macrophages drug effects, Macrophages metabolism, Macrophages immunology, Polygonatum chemistry, Polysaccharides pharmacology, Polysaccharides chemistry, Polysaccharides isolation & purification, Phagocytosis drug effects
- Abstract
A neutral Polygonatum cyrtonema polysaccharide (NPCP) was isolated and purified from Polygonatum cyrtonema by various chromatographic techniques, including DEAE-52 and Sephadex-G100 chromatography. The structure of NPCP was characterized by HPLC, HPGPC, GC-MS, FT-IR, NMR, and SEM. Results showed that NPCP is composed of glucose (55.4%) and galactose (44.6%) with a molecular weight of 3.2 kDa, and the sugar chain of NPCP was →1)-α-D-Glc-(4→1)-β-D-Gal-(3→. In vitro bioactivity experiments demonstrated that NPCP significantly enhanced macrophages proliferation and phagocytosis while inhibiting the M1 polarization induced by LPS as well as the M2 polarization induced by IL-4 and IL-13 in macrophages. Additionally, NPCP suppressed the secretion of IL-6 and TNF-α in both M1 and M2 cells but promoted the secretion of IL-10. These results suggest that NPCP could serve as an immunomodulatory agent with potential applications in anti-inflammatory therapy.
- Published
- 2024
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