1. Shikonin inhibits adipogenic differentiation via regulation of mir-34a-FKBP1B.
- Author
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Jang, Young Jin, Jung, Chang Hwa, Ahn, Jiyun, Gwon, So Young, and Ha, Tae Youl
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SHIKONIN , *ADIPOGENESIS , *MICRORNA , *GENETIC regulation , *NAPHTHOQUINONE , *LITHOSPERMUM - Abstract
Shikonin is a naturally occurring naphthoquinone pigment and a major constituent present in Lithospermum erythrorhizon . Since microRNAs (miRNAs) are one of the key post-transcriptional regulators of adipogenesis, their manipulation represents a potential new strategy to inhibit adipogenesis. Our aim was to investigate shikonin-dependent inhibition of adipogenesis with an emphasis on miRNA-related processes. Mir-34a increased during induced adipogenesis, and this was suppressed in the presence of shikonin. mRNA expression of FKBP1B, a suggested target of mir-34a according to bioinformatics studies, decreased during adipogenesis, but was recovered by shikonin treatment, which reversely correlated with mir-34a expression. A mir-34a inhibitor suppressed MDI-induced adipogenesis by blocking PPARγ and C/EBPα expression, while suppression of mir-34a recovered MDI-induced down-regulation of FKBP1B expression. A mir-34a mimic decreased FKBP1B mRNA expression in 3T3-L1 preadipocytes. We also observed that mir-34a bound directly to the 3′-untranslated region of FKBP1B. Finally, FKBP1B overexpression attenuated MDI-induced adipogenesis, PPARγ, and C/EBPα expression. These results suggest that mir-34a regulates adipogenesis by targeting FKBP1B expression. Our findings reveal that shikonin prevents adipogenesis by blocking the mir-34a-FKBP1B pathway which represents a promising potential target for preventing obesity. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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