1. Chemical and Biological Investigation of Ochrosia elliptica Labill. Cultivated in Egypt
- Author
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Tzvetomira Tzanova, Emilie Evain-Bana, Essam Abdel-Sattar, Denyse Bagrel, Stéphanie Philippot, Dalia A. Al-Mahdy, Mohamed S. Hifnawy, and Riham A. El-Shiekh
- Subjects
Pharmacology ,Ochrosia elliptica ,010405 organic chemistry ,Organic Chemistry ,Plant Science ,Biology ,biology.organism_classification ,01 natural sciences ,Cdc25s ,0104 chemical sciences ,lcsh:QK1-989 ,lcsh:Chemistry ,lcsh:QD241-441 ,010404 medicinal & biomolecular chemistry ,lcsh:QD1-999 ,lcsh:Organic chemistry ,lcsh:Botany ,Drug Discovery ,Botany ,cytotoxicity ,methoxy ellipticine ,anti-inflammatory - Abstract
The phytochemical investigation of Ochrosia eliptica leaves resulted in isolation and identification of eight compounds; lupeol, lupeol acetate, uvaol, ursolic acid, β-sitosterol glucoside, rutin, 8-methoxy and 9-methoxy ellipticine . The ethanolic extract and fractions were studied for their cytotoxic, antioxidant and anti-inflammatory activities. The cytotoxic activity was performed on human mammary adenocarcinoma (MCF7), its multidrug-resistant counterpart (VCREMS), estrogen receptor negative human metastatic breast adenocarcinoma cells (MDA-MB-231) and the non-cancerous, immortalized by telomerase, human breast epithelial cell line (hTERT-HME1). Additionally, the inhibitory potential on Cdc25s proteins was determined. The results showed that the dichloromethane (DCM) fraction and the major alkaloid; 9-methoxyellipticine exhibited high inhibitory activity against all tested cell lines particularly MCF7 and VCREMS cell lines, whereas t he DCM fraction showed a significant inhibitory action on Cdc25 A isoform. In contrast, the n-butanol fraction and 9-methoxyellipticine displayed the highest antioxidant potential. The DCM fraction showed significant anti-inflammatory activity compared to indomethacin. This work comprises the first comprehensive work to be conducted on O. elliptica leaves showing its potential in multiple biological activities .
- Published
- 2017