1. Programmed Adsorption and Release of Proteins in a Microfluidic Device.
- Author
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Huber, Dale L., Manginell, Ronald P., Samara, Michael A., Kim, Byung-Il, and Bunker, Bruce C.
- Subjects
- *
DENATURATION of proteins , *PROTEINS , *POLYMERS , *PROTEOMICS - Abstract
A microfluidic device has been developed that can adsorb proteins from solution, hold them with negligible denaturation, and release them on command. The active element in the device is a 4-nanometer-thick polymer film that can be thermally switched between an antifouling hydrophilic state and a protein-adsorbing state that is more hydrophobic. This active polymer has been integrated into a microfluidic hot plate that can be programmed to adsorb and desorb protein monolayers in less than 1 second. The rapid response characteristics of the device can be manipulated for proteomic functions, including preconcentration and separation of soluble proteins on an integrated fluidics chip. [ABSTRACT FROM AUTHOR]
- Published
- 2003
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