1. Aminophenylboronic-Acid-Conjugated Polyacrylic Acid--Mn -Doped ZnS Quantum Dot for Highly Sensitive Discrimination of Glycoproteins.
- Author
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Li-Jia Sang and He-Fang Wang
- Subjects
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GLYCOPROTEINS , *POLYACRYLIC acid , *GLYCANS , *QUANTUM dots , *SENSOR arrays , *PHOSPHORESCENCE , *LIGHT scattering - Abstract
Discrimination of glycoproteins with different glycans is a significant but difficult issue. We presented here a new strategy for strengthening the discrimination of glycoproteins by introducing a new signaling channel, fluorescence polarization (FP), into a "single probe with three signaling channels" sensor array. The single probe was aminophenylboronic-acid-conjugated poly (acrylic acid)-Mndoped ZnS quantum dots, and the three signaling channels were FP, room temperature phosphorescence and light scattering. Ten glycoproteins, including ovalbumin, fibrinogen, transferrin, horseradish peroxidase, vascular endothelial growth factor, immunoglobulin G, avidin, hyaluronidase, cellulase R- 10, and glucose oxidase, were involved for evaluating the discriminating capability. The introduction of the FP signaling channel improved the discriminating power of the sensor array, so that the 10 glycoproteins at 0.15 μM could be well discriminated both in PBS buffer and in the presence of human serum sample. The identification accuracy of the unknown samples was 96.25% (77 out of 80) at the 0.15 μM level and 97.50% (78 out of 80) at the 0.2 μM level. The integration of the signaling patterns with different responsive principles was demonstrated as the promising way to enhance the discrimination power of the single-probebased sensor arrays. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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