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Your search keyword '"Jiaofeng Li"' showing total 2 results
Start Over Author "Jiaofeng Li" Publisher american chemical society (acs)
2 results on '"Jiaofeng Li"'

1. Palladium-Triggered Chemical Rescue of Intracellular Proteins via Genetically Encoded Allene-Caged Tyrosine

Author

Xin Wang, Jie Li, Jiaofeng Li, Siqi Zheng, Gong Zhang, Zhi Lin, Peng Chen, Zhaoyue Zhang, Yanjun Liu, and Jie Wang

Subjects
Stereochemistry, Bacterial Toxins, Lysine, 010402 general chemistry, 01 natural sciences, Biochemistry, Catalysis, chemistry.chemical_compound, Colloid and Surface Chemistry, Humans, Moiety, Taq Polymerase, Tyrosine, chemistry.chemical_classification, Antigens, Bacterial, 010405 organic chemistry, HEK 293 cells, General Chemistry, 0104 chemical sciences, Alkadienes, HEK293 Cells, Enzyme, chemistry, Bioorthogonal chemistry, Palladium, Taq polymerase, Proto-oncogene tyrosine-protein kinase Src
Abstract

Chemical de-caging has emerged as an attractive strategy for gain-of-function study of proteins via small-molecule reagents. The previously reported chemical de-caging reactions have been largely centered on liberating the side chain of lysine on a given protein. Herein, we developed an allene-based caging moiety and the corresponding palladium de-caging reagents for chemical rescue of tyrosine (Tyr) activity on intracellular proteins. This bioorthogonal de-caging pair has been successfully applied to unmask enzymatic Tyr sites (e.g., Y671 on Taq polymerase and Y728 on Anthrax lethal factor) as well as the post-translational Tyr modification site (Y416 on Src kinase) in vitro and in living cells. Our strategy provides a general platform for chemical rescue of Tyr-dependent protein activity inside cells.

Published
2016

2. Paired Design of dCas9 as a Systematic Platform for the Detection of Featured Nucleic Acid Sequences in Pathogenic Strains

Author

Xiaohan Zhang, Xuejin Zhao, Jiaofeng Li, Sida Cheng, Long Qian, Yixuan Ye, Haoqian Zhang, Wenchao Liu, Dongming Liu, Yiming Dong, Chunbo Lou, Yihao Zhang, Yu Wang, Luze Xu, Hang Li, Xiang Li, Cui Hua Liu, Beining Wang, Qi Ouyang, Pingping Lin, and Weijia Wei

Subjects
DNA, Bacterial, 0301 basic medicine, Luminescence, Biomedical Engineering, Biology, Sensitivity and Specificity, Biochemistry, Genetics and Molecular Biology (miscellaneous), Genome, Paired design, 03 medical and health sciences, Nucleic Acids, Luciferase, Luciferases, Sequence (medicine), Genetics, Base Sequence, Colocalization, Mycobacterium tuberculosis, General Medicine, Endonucleases, Dna detection, 030104 developmental biology, Nucleic acid, Mycobacterium tuberculosis DNA, CRISPR-Cas Systems, Genome, Bacterial
Abstract

We developed an in vitro DNA detection system using a pair of dCas9 proteins linked to split halves of luciferase. Luminescence was induced upon colocalization of the reporter pair to a ∼44 bp target sequence defined by sgRNAs. We used the system to detect Mycobacterium tuberculosis DNA with high specificity and sensitivity. The reprogrammability of dCas9 was further leveraged in an array design that accesses sequence information across the entire genome.

Published
2016

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