1. Erythrocyte n-6 Polyunsaturated Fatty Acids, Gut Microbiota, and Incident Type 2 Diabetes: A Prospective Cohort Study.
- Author
-
Miao Z, Lin JS, Mao Y, Chen GD, Zeng FF, Dong HL, Jiang Z, Wang J, Xiao C, Shuai M, Gou W, Fu Y, Imamura F, Chen YM, and Zheng JS
- Subjects
- Adult, Aged, Arachidonic Acid blood, Biomarkers blood, China epidemiology, Diabetes Mellitus, Type 2 blood, Diabetes Mellitus, Type 2 microbiology, Erythrocytes chemistry, Fatty Acids, Omega-6 analysis, Fatty Acids, Unsaturated analysis, Fatty Acids, Unsaturated blood, Female, Humans, Incidence, Male, Middle Aged, Prospective Studies, Diabetes Mellitus, Type 2 epidemiology, Erythrocytes metabolism, Fatty Acids, Omega-6 blood, Gastrointestinal Microbiome physiology
- Abstract
Objective: To examine the association of erythrocyte n-6 polyunsaturated fatty acid (PUFA) biomarkers with incident type 2 diabetes and explore the potential role of gut microbiota in the association., Research Design and Methods: We evaluated 2,731 participants without type 2 diabetes recruited between 2008 and 2013 in the Guangzhou Nutrition and Health Study (Guangzhou, China). Case subjects with type 2 diabetes were identified with clinical and biochemical information collected at follow-up visits. Using stool samples collected during the follow-up in the subset ( n = 1,591), 16S rRNA profiling was conducted. Using multivariable-adjusted Poisson or linear regression, we examined associations of erythrocyte n-6 PUFA biomarkers with incident type 2 diabetes and diversity and composition of gut microbiota., Results: Over 6.2 years of follow-up, 276 case subjects with type 2 diabetes were identified (risk 0.10). Higher levels of erythrocyte γ-linolenic acid (GLA), but not linoleic or arachidonic acid, were associated with higher type 2 diabetes incidence. Comparing the top to the bottom quartile groups of GLA levels, relative risk was 1.72 (95% CI 1.21, 2.44) adjusted for potential confounders. Baseline GLA was inversely associated with gut microbial richness and diversity (α-diversity, both P < 0.05) during follow-up and significantly associated with microbiota β-diversity ( P = 0.002). α-Diversity acted as a potential mediator in the association between GLA and type 2 diabetes ( P < 0.05). Seven genera ( Butyrivibrio , Blautia , Oscillospira , Odoribacter , S24-7 other , Rikenellaceae other , and Clostridiales other ) were enriched in quartile 1 of GLA and in participants without type 2 diabetes., Conclusions: Relative concentrations of erythrocyte GLA were positively associated with incident type 2 diabetes in a Chinese population and also with gut microbial profiles. These results highlight that gut microbiota may play an important role linking n-6 PUFA metabolism and type 2 diabetes etiology., (© 2020 by the American Diabetes Association.)
- Published
- 2020
- Full Text
- View/download PDF