1. Comparative study of ROR2 and WNT5a expression in squamous/adenosquamous carcinoma and adenocarcinoma of the gallbladder
- Author
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Lingxiang Wang, Ziru Liu, Qiong Zou, Zhulin Yang, Li Xiong, Zhengchun Wu, Hua Zhao, Xiongying Miao, Kuijie Liu, and Daiqiang Li
- Subjects
Male ,0301 basic medicine ,Oncology ,Pathology ,Time Factors ,Clinical Trials Study ,Kaplan-Meier Estimate ,0302 clinical medicine ,Risk Factors ,Gallbladder adenosquamous carcinoma ,WNT5a ,ROR2 ,Aged, 80 and over ,Gallbladder Adenosquamous Carcinoma ,Gallbladder adenocarcinoma ,Gastroenterology ,Gallbladder squamous carcinoma ,Cell Differentiation ,General Medicine ,Middle Aged ,Immunohistochemistry ,Up-Regulation ,WNT5A ,Treatment Outcome ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,embryonic structures ,Adenocarcinoma ,Female ,Gallbladder Neoplasms ,Adult ,China ,medicine.medical_specialty ,Adenosquamous carcinoma ,Receptor Tyrosine Kinase-like Orphan Receptors ,Wnt-5a Protein ,Carcinoma, Adenosquamous ,03 medical and health sciences ,Internal medicine ,Biomarkers, Tumor ,medicine ,Humans ,Neoplasm Invasiveness ,Aged ,Neoplasm Staging ,Proportional Hazards Models ,Chi-Square Distribution ,business.industry ,Gallbladder ,medicine.disease ,digestive system diseases ,body regions ,stomatognathic diseases ,030104 developmental biology ,Multivariate Analysis ,sense organs ,Gallbladder Neoplasm ,business - Abstract
AIM To investigate the expression and clinical pathological significance of ROR2 and WNT5a in gallbladder squamous/adenosquamous carcinoma (SC/ASC) and adenocarcinoma (AC). METHODS EnVision immunohistochemistry was used to stain for ROR2 and WNT5a in 46 SC/ASC patients and 80 AC patients. RESULTS Poorly differentiated AC among AC patients aged > 45 years were significantly more frequent compared with SC/ASC patients, while tumors with a maximal diameter > 3 cm in the SC/ASC group were significantly more frequent compared with the AC group. Positive ROR2 and WNT5a expression was significantly lower in SC/ASC or AC with a maximal mass diameter ≤ 3 cm, a TNM stage of I + II, no lymph node metastasis, no surrounding invasion, and radical resection than in patients with a maximal mass diameter > 3 cm, TNM stage IV, lymph node metastasis, surrounding invasion, and no resection. Positive ROR2 expression in patients with highly differentiated SC/ASC was significantly lower than in patients with poorly differentiated SC/ASC. Positive ROR2 and WNT5a expression levels in highly differentiated AC were significantly lower than in poorly differentiated AC. Kaplan-Meier survival analysis showed that differentiation degree, maximal mass diameter, TNM stage, lymph node metastasis, surrounding invasion, surgical procedure and the ROR2 and WNT5a expression levels were closely related to average survival of SC/ASC or AC. The survival of SC/ASC or AC patients with positive expression of ROR2 and WNT5a was significantly shorter than that of patients with negative expression results. Cox multivariate analysis revealed that poor differentiation, a maximal diameter of the mass ≥ 3 cm, TNM stage III or IV, lymph node metastasis, surrounding invasion, unresected surgery and positive ROR2 or WNT5a expression in the SC/ASC or AC patients were negatively correlated with the postoperative survival rate and positively correlated with mortality, which are risk factors and independent prognostic predictors. CONCLUSION SC/ASC or AC patients with positive ROR2 or WNT5a expression generally have a poor prognosis.
- Published
- 2017