1. Implication of fermentable carbohydrates targeting the gut microbiota on conjugated linoleic acid production in high-fat-fed mice
- Author
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Tom Van de Wiele, Evelyne M. Dewulf, Audrey M. Neyrinck, Patrice D. Cani, Luc De Vuyst, Yvan Larondelle, Frédéric Moens, Sam Possemiers, Fabienne De Backer, Céline Druart, Nathalie M. Delzenne, UCL - SST/ISV - Institut des sciences de la vie, UCL - SSS/LDRI - Louvain Drug Research Institute, and Industrial Microbiology more...
- Subjects
Male ,medicine.medical_treatment ,Conjugated linoleic acid ,Linoleic acid ,Carbohydrates ,Medicine (miscellaneous) ,Vaccenic acid ,Gut microbiota ,Biology ,Gut flora ,Carbohydrate metabolism ,digestive system ,Gene Expression Regulation, Enzymologic ,Mice ,chemistry.chemical_compound ,Fatty Acids, Omega-6 ,medicine ,Animals ,Linoleic Acids, Conjugated ,Food science ,Prebiotic supplementation ,chemistry.chemical_classification ,Nutrition and Dietetics ,Bacteria ,Rumenic acid ,Prebiotic ,food and beverages ,biology.organism_classification ,Dietary Fats ,Intestines ,Mice, Inbred C57BL ,high-fat diet ,Prebiotics ,Liver ,chemistry ,Fermentation ,Carbohydrate Metabolism ,Conjugated linoleic acids ,Stearoyl-CoA Desaturase ,Polyunsaturated fatty acid - Abstract
In vitro experiments have shown that isolated human gut bacteria are able to metabolise PUFA into conjugated PUFA like conjugated linoleic acids (CLA). The hypothesis of the present paper was that high-fat (HF) diet feeding and supplementation with fermentable carbohydrates that have prebiotic properties modulate the in vivo production of CLA by the mouse gut microbiota. Mice were treated for 4 weeks as follows: control (CT) groups were fed a standard diet; HF groups were fed a HF diet rich in linoleic acid (18 : 2n-6); the third groups were fed with the HF diet supplemented with either inulin-type fructans (HF-ITF) or arabinoxylans (HF-Ax). HF diet feeding increased rumenic acid (cis-9, trans-11-18 : 2 CLA) content both in the caecal and liver tissues compared with the CT groups. ITF supplementation had no major effect compared with the HF diet whereas Ax supplementation increased further rumenic acid (cis-9, trans-11-18 : 2 CLA) in the caecal tissue. These differences between both prebiotics may be linked to the high fat-binding capacity of Ax that provides more substrates for bacterial metabolism and to differential modulation of the gut microbiota (specific increase in Roseburia spp. in HF-Ax v. HF). In conclusion, these experiments supply the proof of concept that the mouse gut microbiota produces CLA in vivo, with consequences on the level of CLA in the caecal and liver tissues. We postulate that the CLA-producing bacteria could be a mediator to consider in the metabolic effects of both HF diet feeding and prebiotic supplementation. more...
- Published
- 2013
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