1. A single-cell atlas of mouse lung development.
- Author
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Negretti, Nicholas M., Plosa, Erin J., Benjamin, John T., Schuler, Bryce A., Habermann, A. Christian, Jetter, Christopher S., Gulleman, Peter, Bunn, Claire, Hackett, Alice N., Ransom, Meaghan, Taylor, Chase J., Nichols, David, Matlock, Brittany K., Guttentag, Susan H., Blackwell, Timothy S., Banovich, Nicholas E., Kropski, Jonathan A., and Sucre, Jennifer M. S.
- Subjects
LUNG development ,CELL populations ,ENDOTHELIAL cells ,IN situ hybridization ,MICE ,LUNGS - Abstract
Lung organogenesis requires precise timing and coordination to effect spatial organization and function of the parenchymal cells. To provide a systematic broad-based view of the mechanisms governing the dynamic alterations in parenchymal cells over crucial periods of development, we performed a single-cell RNA-sequencing timeseries yielding 102,571 epithelial, endothelial and mesenchymal cells across nine time points from embryonic day 12 to postnatal day 14 in mice. Combining computational fate-likelihood prediction with RNA in situ hybridization and immunofluorescence, we explore lineage relationships during the saccular to alveolar stage transition. The utility of this publicly searchable atlas resource (www.sucrelab.org/lungcells) is exemplified by discoveries of the complexity of type 1 pneumocyte function and characterization of mesenchymal Wnt expression patterns during the saccular and alveolar stages - wherein major expansion of the gas-exchange surface occurs. We provide an integrated view of cellular dynamics in epithelial, endothelial and mesenchymal cell populations during lung organogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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