1. Effectiveness of digital PCR for MYD88L265P detection in vitreous fluid for primary central nervous system lymphoma diagnosis
- Author
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Ming Guan, Bobin Chen, Xinju Zhang, Yanchun Ma, Tianling Ding, and Kun Chen
- Subjects
0301 basic medicine ,Cancer Research ,Pathology ,medicine.medical_specialty ,ddPCR ,cerebrospinal fluid ,03 medical and health sciences ,0302 clinical medicine ,Cerebrospinal fluid ,Immunology and Microbiology (miscellaneous) ,hemic and lymphatic diseases ,medicine ,Digital polymerase chain reaction ,central nervous system lymphoma ,business.industry ,vitreous aspirates ,Not Otherwise Specified ,Primary central nervous system lymphoma ,Cancer ,General Medicine ,Articles ,medicine.disease ,Lymphoma ,030104 developmental biology ,Real-time polymerase chain reaction ,030220 oncology & carcinogenesis ,MYD88L265P mutation ,Mutation (genetic algorithm) ,business - Abstract
Primary central nervous system lymphoma (PCNSL) is a rare type of primary extranodal lymphoma (PEL). MYD88L265P mutation has been observed in up to 75% of PCNSL cases, however, the validity and sensitivity of digital PCR in detecting this mutation remains to be elucidated. A total of 44 PCNSL patients, 15 diffuse large B-cell lymphoma not otherwise specified (DLBCL-NOS) patients and 13 other PEL patients were enrolled in the present study. The abilities of reverse transcription quantitative PCR (RT-qPCR) and droplet digital PCR (ddPCR) to detect the MYD88L265P mutation in cerebral spinal fluid (CSF) samples were compared. The results suggested that ddPCR showed superior mutation detection sensitivity when compared with RT-qPCR (58 vs. 15%; P
- Published
- 2020