1. Hypermethylation of DLX4 predicts poor clinical outcome in patients with myelodysplastic syndrome.
- Author
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Zhang, Ting-juan, Zhou, Jing-dong, Yang, Dong-qin, Wang, Yu-xin, Yao, Dong-ming, Ma, Ji-chun, Wen, Xiang-mei, Guo, Hong, Lin, Jiang, and Qian, Jun
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METHYLATION , *HOMEOBOX genes , *MYELODYSPLASTIC syndromes , *HEALTH outcome assessment , *POLYMERASE chain reaction , *KARYOTYPES , *PATIENTS , *PROGNOSIS - Abstract
Background: Hypermethylation of DLX4 (distal-less homeobox 4) has been disclosed in a variety of cancers. Our work was aimed to examine the pattern of DLX4 methylation and further investigate its clinical relevance in patients with myelodysplastic syndrome (MDS). Methods: Real-time quantitative methylation-specific PCR and bisulfite sequencing PCR were carried out to detect the level of DLX4 methylation. Clinical significance of DLX4 methylation was analyzed between the DLX4 hypermethylated and non-hypermethylated patients. Results: DLX4 was significantly hypermethylated in MDS patients than controls (p<0.001). No significant differences were observed between the hypermethylated and non-hypermethylated MDS patients in white blood cells, platelets, age, WHO classifications, FAB classifications, IPSS risks, and common gene mutations (p>0.05). However, DLX4 hypermethylated patients tended to have higher hemoglobin (HB) than DLX4 non-hypermethylated patients (p=0.079). Moreover, there was a trend that male patients, poor karyotype patients, and IPSS Int-2/High patients had a higher frequency of DLX4 hypermethylation (p=0.067, 0.065, and 0.068). DLX4 hypermethylated patients had significantly shorter overall survival than DLX4 non-hypermethylated patients (p=0.004). Multivariate analysis confirmed the prognostic value of DLX4 methylation in MDS patients (p<0.001). Conclusions: Our study indicated that DLX4 hypermethylation was a frequent event and acted as an independent prognostic biomarker in de novo MDS patients. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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