1. Generation of a human induced pluripotent stem cell line (HMGUi002-A) from a healthy male individual.
- Author
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Wang X, Malinowski AR, Beckenbauer J, Siehler J, Blöchinger A, Meitinger T, Häring HU, Staiger H, Burtscher I, and Lickert H
- Subjects
- Cell Differentiation genetics, Cell Differentiation physiology, Cell Lineage, Cells, Cultured, Cellular Reprogramming genetics, Cellular Reprogramming physiology, Humans, Male, Induced Pluripotent Stem Cells cytology
- Abstract
Induced pluripotent stem cells (iPSCs) can be used to generate different somatic cell types in vitro, including insulin-producing pancreatic β-cells. Here, we have generated iPSCs from a healthy male individual using an episomal reprogramming method. The resulting iPSCs are integration-free, have a normal karyotype and are pluripotent in vitro and in vivo. Furthermore, we show that this iPSC line can be differentiated into pancreatic lineage cells. Taken together, this iPSC line will be useful to test differentiation protocols towards β-cell as well as other cell types and will also serve as a control for drug development and disease modelling studies., (Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2019
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