1. Development of immunoassay methods based on monoclonal antibody and its application in the determination of cadmium ion.
- Author
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Ling S, Zhao Q, Iqbal MN, Dong M, Li X, Lin M, Wang R, Lei F, He C, and Wang S
- Subjects
- Enzyme-Linked Immunosorbent Assay, Immunoassay, Limit of Detection, Antibodies, Monoclonal, Cadmium
- Abstract
Owing to the threat of cadmium (Cd
2+ ) to public health, it is an urgent demand to develop effective, sensitive, and rapid methods for the detection of cadmium. In this study, indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and immunochromatographic test strips (ICTS) were established for the determination of Cd2+ based on the obtained mAb with high specificity and high affinity (Kaff = 3.0 × 109 L/moL). The linear range of ic-ELISA detection was 0.03-1.11 ng/mL and 50% inhibitive concentration (IC50) of cadmium ion was determined to be 0.15 ng/mL. The visual limit of detection (vLOD) of the AuNS-based strip was 0.375 ng/mL. The vLOD of AuNF-based strip using higher intensity reporter determined to be 0.03 ng/mL, which was enhanced 12 times compared to the traditional strip. In summary, the developed immunoassays based on mAb shows great potential for monitoring the cadmium ion in environmental samples., (Copyright © 2021 Elsevier B.V. All rights reserved.)- Published
- 2021
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