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3 results on '"Hong, Die"'

1. Danshen can interact with intestinal bacteria from normal and chronic renal failure rats

Author

Zhenhua Zhu, Dawei Qian, Sheng Guo, Jianming Guo, Jin-Ao Duan, Hong-Die Cai, Li Yonghui, Yue Zhu, and Shulan Su

Subjects
Male, 0301 basic medicine, Glucuronidation, Salvia miltiorrhiza, Intestinal bacteria, RM1-950, Pharmacology, Salviae miltiorrhizae radix et rhizoma, Peptostreptococcaceae, Rats, Sprague-Dawley, 03 medical and health sciences, 0302 clinical medicine, Clostridium, Butyrivibrio, Chronic renal failure, Animals, Demethylation, biology, Chemistry, Ruminococcus, Pseudomonas, General Medicine, biology.organism_classification, Gastrointestinal Microbiome, Rats, 030104 developmental biology, 030220 oncology & carcinogenesis, Salvianolic acid, Tanshinone, Kidney Failure, Chronic, Peptococcus, Therapeutics. Pharmacology, Biomarkers
Abstract

Danshen (Salviae Miltiorrhizae Radix et Rhizoma, SMR) has been used as a traditional Chinese medicine in clinic for treatment of coronary heart diseases. Previous works have shown that the chronic renal failure (CRF) is closely related to changes of intestinal bacteria. The aim is to explore the interaction between active components of SMR and intestinal bacteria from normal and CRF rats. The changes of intestinal bacteria were evaluated among normal rats, CRF model rats and SMR-treated rats via 16S rRNA gene sequencing technology. UPLC-QTOF/MS was applied for the analysis and identification of metabolites. RESULTS: Results showed that the following intestinal bacteria varied significantly in CRF rats, including Mucispirillum, Kurthia, Clostridium, Blautia, Butyrivibrio, Shuttleworthia, Peptococcus, Ruminococcus, Bradyrhizobium, Methylobacterium, Azospirillum, Thalassospira, Methylophilus, Pseudomonas, peptostreptococcaceae and bacteroidales. The ethanol extract of SMR (DS) significantly regulated Shuttleworthia, peptostreptococcaceae and Pseudomonas, while the water extract (DSS) significantly affected Peptococcus, peptostreptococcaceae and Ruminococcus. Methylation, demethylation, dehydrogenation, hydrogenation and hydroxylation were the major metabolic transformation of tanshinones in vitro by intestinal bacteria. Glucuronidation, methylation and hydrogenation were the main metabolic transformation of salvianolic acids. These results showed that the bioactive components of SMR, including tanshinones and salvianolic acids, might exert the medical effect via regulation intestinal bacteria.

Published
2019

2. Salvia miltiorrhiza protects against diabetic nephropathy through metabolome regulation and wnt/β-catenin and TGF-β signaling inhibition.

Author

Xiang X, Cai HD, Su SL, Dai XX, Zhu Y, Guo JM, Yan H, Guo S, Gu W, Qian DW, Tang ZS, and Duan JA

Subjects
Animals, Diabetic Nephropathies metabolism, Diabetic Nephropathies pathology, Kidney drug effects, Kidney metabolism, Kidney pathology, Male, Rats, Sprague-Dawley, Signal Transduction, Transforming Growth Factor beta metabolism, Wnt Proteins metabolism, beta Catenin metabolism, Diabetic Nephropathies drug therapy, Metabolome drug effects, Plant Extracts pharmacology, Plant Extracts therapeutic use, Protective Agents pharmacology, Protective Agents therapeutic use, Salvia miltiorrhiza
Abstract

Diabetic nephropathy (DN) is a complication of diabetes that is caused by uncontrolled high blood sugar. It has been reported that Salvia miltiorrhiza (SM) possesses the ability to prevent kidney damage, although the mechanisms remain unclear. The study was to investigate whether and how SM improved DN injury via regulation of metabolome and the molecular mechanisms. In this study, SD rats were fed a high glucose / high fat diet accompanied by 0.5% glucose water. Three weeks later, the rats were given one intraperitoneal injection of 30 mg/kg STZ each day for three days for DN model. The biochemical indicators and metabolomics of plasma, urine and renal tissue were analyzed. Then the western blotting analysis of renal tissue and glomerular mesangial cells were investigated. The results showed that Salvia miltiorrhiza extracts improved the renal injury and regulation of abnormal glycolipid metabolism. The metabolites in serum, urine and renal tissues have been changed significantly. The involved metabolic pathways mainly include phospholipid, arachidonic acid, and pyrimidine metabolisms. Meanwhile, SM inhibited the relative expression levels of wnt4, β-catenin and TGF-β in renal tissue and high-glucose induced glomerular mesangial cells., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Published
2019
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3. Inhibition of cervical cancer cell growth in vitro and in vivo by lentiviral-vector mediated shRNA targeting the common promoter of HPV16 E6 and E7 oncogenes.

Author

Zhou J, Li B, Peng C, Wang F, Fu Z, Zhou C, Hong D, Ye F, Lü W, and Xie X

Subjects
Animals, Cell Line, Tumor, Female, Genetic Therapy, Genetic Vectors genetics, Genetic Vectors physiology, Human papillomavirus 16 metabolism, Humans, Lentivirus genetics, Lentivirus physiology, Mice, Mice, Inbred BALB C, Mice, Nude, Oncogene Proteins, Viral metabolism, Papillomavirus E7 Proteins metabolism, RNA, Small Interfering metabolism, RNA, Small Interfering therapeutic use, Repressor Proteins metabolism, Uterine Cervical Neoplasms physiopathology, Uterine Cervical Neoplasms therapy, Cell Proliferation, Gene Silencing, Human papillomavirus 16 genetics, Oncogene Proteins, Viral genetics, Papillomavirus E7 Proteins genetics, Promoter Regions, Genetic, RNA, Small Interfering genetics, Repressor Proteins genetics, Uterine Cervical Neoplasms virology
Abstract

Deregulated expression of high-risk human papillomavirus oncogenes (E6 and E7) is a pivotal event for pathogenesis and progression in cervical cancer. Both viral oncogenes are therefore regarded as ideal therapeutic targets. Small interfering RNAs (siRNA) or double-stranded RNAs can knock down target genes effectively through siRNA-induced transcriptional gene silencing (TGS). Here, we established lentiviral-vector mediated shRNA (LV-shRNA) targeting common promoter of HPV16 E6/E7 and targeting E6 transcript, transduced the lentiviral construct into cervical HPV16-positive cell lines Siha and Caski, then selected and established stably transduced monoclonal cell lines. The results showed that LV-shRNA targeting promoter, as well as targeting E6 transcript, effectively knocked down E6 and E7 expression, resulted in accumulation of p53 and pRB protein and decrease of MCM7 and p16 protein, and consequently remarkably reduced the abilities of proliferation and invasiveness of cervical cancers cells in vitro. Then we inoculated subcutaneously those monoclonal cells into nude mice to establish the transplanted tumor animal models, and found dramatically inhibited tumorigenesis and growth, as well as prolonged survival time of mice incubated by cells with LV-shRNA targeting promoter and E6 transcript. Our results may provide evidence for application of LV-shRNA targeting HR-HPV key oncogenes, as a new treatment strategy, in cervical and other HPV-associated cancer therapy., (Copyright © 2013 Elsevier B.V. All rights reserved.)

Published
2013
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