24 results on '"Ma, Han"'
Search Results
2. Insights into the response of electroactive biofilm with petroleum hydrocarbons degradation ability to quorum sensing signals.
- Author
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Xue J, Ma H, Dong X, Shi K, Zhou X, Qiao Y, Gao Y, Liu Y, Feng Y, and Jiang Q
- Subjects
- Bacteria metabolism, Bacteria genetics, Electrochemical Techniques, Bioelectric Energy Sources, Biofilms drug effects, Quorum Sensing, Petroleum metabolism, Hydrocarbons metabolism, Biodegradation, Environmental
- Abstract
Bioelectrochemical technologies based on electroactive biofilms (EAB) are promising for petroleum hydrocarbons (PHs) remediation as anode can serve as inexhaustible electron acceptor. However, the toxicity of PHs might inhibit the formation and function of EABs. Quorum sensing (QS) is ideal for boosting the performance of EABs, but its potential effects on reshaping microbial composition of EABs in treating PHs are poorly understood. Herein, two AHL signals, C4-HSL and C12-HSL, were employed to promote EABs for PHs degradation. The start-times of AHL-mediated EABs decreased by 18-26%, and maximum current densities increased by 28-63%. Meanwhile, the removal of total PHs increased to over 90%. AHLs facilitate thicker and more compact biofilm as well as higher viability. AHLs enhanced the electroactivity and direct electron transfer capability. The total abundance of PH-degrading bacteria increased from 52.05% to 75.33% and 72.02%, and the proportion of electroactive bacteria increased from 26.14% to 62.72% and 63.30% for MFC-C4 and MFC-C12. Microbial networks became more complex, aggregated, and stable with addition of AHLs. Furthermore, AHL-stimulated EABs showed higher abundance of genes related to PHs degradation. This work advanced our understanding of AHL-mediated QS in maintaining the stable function of microbial communities in the biodegradation process of petroleum hydrocarbons., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
- Full Text
- View/download PDF
3. Using ultrasonic-assisted sodium bicarbonate treatment to improve the gel and rheological properties of reduced-salt pork myofibrillar protein.
- Author
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Wu ZW, Shang XY, Hou Q, Xu JG, Kang ZL, and Ma HJ
- Subjects
- Animals, Swine, Sodium Bicarbonate, Ultrasonics, Sodium Chloride, Sodium Chloride, Dietary, Rheology, Water chemistry, Pork Meat, Red Meat analysis
- Abstract
To study the impact of ultrasonic duration (0, 30, and 60 min) and sodium bicarbonate concentration (0% and 0.2%) on the gel properties of reduced-salt pork myofibrillar protein, the changes in cooking yield, colour, water retention, texture properties, and dynamic rheology were investigated. The findings revealed that added sodium bicarbonate significantly increased (P < 0.05) cooking yield, hardness, springiness, and strength of myofibrillar protein while reducing centrifugal loss. Furthermore, the incorporation of sodium bicarbonate led to a significant decrease in L
⁎ , a⁎ , b⁎ , and white values of cooked myofibrillar protein; these effects were further amplified with increasing ultrasonic duration (P < 0.05). Additionally, storage modulus (G') significantly increased for myofibrillar protein treated with ultrasonic-assisted sodium bicarbonate treatment resulting in a more compact gel structure post-cooking. In summary, the results demonstrated that ultrasonic-assisted sodium bicarbonate treatment could enhance the tightness of reduced-salt myofibrillar protein gel structure while improving the water retention and texture properties., Competing Interests: Declaration of competing interest The authors (Zhong-Wei Wu, Xue-Yan Shang, Qin Hou, Jing-Guo Xu, Zhuang-Li Kang, Han-Jun Ma) declare that there are no financial interests or personal relationships that will influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
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4. Presentation approaches for enhancing interpretability of patient-reported outcomes in meta-analyses: a systematic survey of Cochrane reviews.
- Author
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Zeng L, Yao L, Wang Y, Han MA, Granholm A, Nampo F, Tadayon B, Ni X, Zhang L, Devji T, and Guyatt G
- Subjects
- Humans, Retrospective Studies, Surveys and Questionnaires, Meta-Analysis as Topic, Systematic Reviews as Topic, Patient Reported Outcome Measures
- Abstract
Objectives: To systematically survey Cochrane reviews' approaches to calculating, presenting, and interpreting pooled estimates of patient-reported outcome measures (PROMs)., Study Design and Setting: We retrospectively selected 200 Cochrane reviews that met the eligibility criteria. Two researchers independently extracted the pooled effect measures and approaches for pooling and interpreting the effect measures, reaching consensus through discussions., Results: When primary studies used the same PROM, Cochrane review authors most often used mean differences (MDs) (81.9%) for calculating the pooled effect measures; when primary studies used different PROMs, the review authors often applied standardized mean differences (SMDs) (54.3%). Although in most cases (80.1%) the review authors interpreted the importance of effect, they failed, in 48.5% of the pooled effect measures, to report criteria for categorizing the magnitude of effect. When authors interpreted the importance of the effect, for those with primary studies using the same PROM, they most often referred to the minimally important differences (MIDs) (75.0%); for those with primary studies using different PROMs, the approaches used varied., Conclusion: Cochrane review authors most often used MDs or SMDs for calculating and presenting the pooled effect measures of PROs but often failed to make explicit their criteria for categorizing the magnitude of effect., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2023
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5. Effects of pre-emulsified safflower oil with magnetic field modified soy 11S globulin on the gel, rheological, and sensory properties of reduced-animal fat pork batter.
- Author
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Kang ZL, Xie JJ, Li YP, Song WJ, and Ma HJ
- Subjects
- Animals, Swine, Food Handling, Safflower Oil, Rheology, Magnetic Fields, Fat Substitutes chemistry, Pork Meat, Red Meat, Globulins
- Abstract
In this work, the differences in macrostructure and microstructure, rheology, and storage stability of pre-emulsified safflower oil (PSO) prepared by natural and magnetic field modified soy 11S globulin were analysised. It was concluded that the PSO with magnetic field modified soy 11S globulin (MPSO) has better emulsifying activity and physical stability. The changes in gel quality, oxidational sensitivity, rheological, and sensory properties of pork batters with different substitute ratios (0%, 25%, 50%, 75%, and 100%) of pork back-fat by MPSO with magnetic field modified soy 11S globulin were studied. Compared to the sample without MPSO, pork batter with MPSO showed higher emulsion stability, apparent viscosity, L
⁎ value, springiness, cohesiveness, and expressible moisture, while lower a⁎ value and cooking loss. Moreover, added MPSO could be more uniformly distributed into the meat matrix with smaller holes. With the increase in the replacement proportion of pork back-fat, the hardness, water- and fat-holding capacity, and P21 of pork batter significantly decreased (P < 0.05). As revealed by sensory evaluation and TBARS, using MPSO to substitute for pork back-fat decreased the lipid oxidational sensitivity of pork batter, and without negative effects on the appearance, juiciness and overall acceptability. Overall, it is feasible to apply MPSO as a pork-fat replacer to produce reduced-animal fat pork batter with excellent gel and sensory properties., Competing Interests: Declaration of Competing Interest The authors (Zhuang-Li Kang, Jing-jie Xie, Yan-ping Li, Wan-jie Song, Han-Jun Ma) declare that there are no financial interests or personal relationships that will influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)- Published
- 2023
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6. Hourly personal temperature exposure and heart rate variability: A multi-center panel study in populations at intermediate to high-risk of cardiovascular disease.
- Author
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Li H, Ma H, Li J, Li X, Huang K, Cao J, Li J, Yan W, Chen X, Zhou X, Cui C, Yu X, Liu F, and Huang J
- Subjects
- Humans, Heart Rate, Seasons, Cardiovascular Diseases epidemiology, Temperature, Environmental Exposure
- Abstract
Background: Several studies reported temperature exposure was associated with altered cardiac automatic function, while this effect of temperature on hourly heart rate variability (HRV) among populations with cardiovascular risks was seldom addressed., Methods: We conducted this panel study in four Chinese cities with three repeated visits among 296 participants at intermediate to high-risk of cardiovascular disease (CVD). Real-time temperature level and 24-h ambulatory electrocardiogram were monitored during each seasonal visit. Linear mixed-effects models were used to investigate associations between individual temperature and HRV parameters, and the seasonal effects and circadian effect were also evaluated., Results: We found the overall downward trend of hourly HRV associated with acute exposure to higher temperature. For each 1 °C increment in temperature of 1-3 h prior to HRV measurements (lag 1-3 h), hourly standard deviation of normal-to-normal intervals (SDNN) decreased by 0.38% (95% confidence interval [CI]: 0.22, 0.54), 0.28% (95% CI: 0.12, 0.44), and 0.20% (95% CI: 0.04, 0.36), respectively. Similar inverse associations between temperature and HRV were observed in stratified analyses by temperature level. Inverse associations for cold and warm seasons were also observed, despite some effects gradually decreased and reversed in the warm season as lag times extended. Moreover, HRV showed a more significant reduction with increased temperature during daytime than nighttime. Percent change of hourly SDNN was -0.41% (95% CI: -0.62, -0.21) with 1 °C increment of lag 1 h during daytime, while few obvious changes were revealed during nighttime., Conclusions: Generally, increasing temperature was significantly associated with reduced HRV. Inverse relationships for cold and warm seasons were also observed. Associations during daytime were much more prominent than nighttime. Our findings clarified the relationship of temperature with HRV and provided evidence for prevention approaches to alleviate cardiac automatic dysfunction among populations at intermediate to high-risk of CVD., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2023
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7. Effect of sodium bicarbonate on techno-functional and rheological properties of pale, soft, and exudative (PSE) meat batters.
- Author
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Li YP, Zou XL, Kang ZL, and Ma HJ
- Subjects
- Cooking, Sodium Chloride, Sodium Chloride, Dietary, Sodium Bicarbonate, Meat
- Abstract
In the study, changes in salt-soluble protein (SSP) content, gel properties, rheological characteristic, and microstructure attributes of pale, soft, and exudative (PSE) pork batters with different concentrations of added sodium bicarbonate (0-0.6%) were investigated. The pH, b
⁎ value, SSP content, cooking yield, texture properties, emulsion stability, and G' values at 72 °C significantly increased with the increase in sodium bicarbonate, but the texture properties and G' values of the samples with 0.4% and 0.6% did not significantly different, while the a⁎ value significantly decreased. Moreover, a greater G' value at 72 °C was in agreement with a higher hardness value of meat batter. The microstructure of cooked PSE meat batters with 0% and 0.2% sodium bicarbonate had a dense structure, and samples with 0.4% and 0.6% had some large cavities. In conclusion, the use of sodium bicarbonate can enhance the water holding capacity, texture and rheological properties of PSE meat batters by increasing their pH, SSP content, and emulsifying stability., Competing Interests: Declaration of Competing Interest The authors (Yan-ping Li, Xiao-Li Zou, Zhuang-Li Kang, Han-Jun Ma) declare that there are no financial interests or personal relationships that will influence the work reported in this paper., (Copyright © 2022. Published by Elsevier Ltd.)- Published
- 2022
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8. Causal language use in systematic reviews of observational studies is often inconsistent with intent: a systematic survey.
- Author
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Han MA, Leung G, Storman D, Xiao Y, Srivastava A, Talukdar JR, El Dib R, Morassut RE, Zeraatkar D, Johnston BC, and Guyatt G
- Subjects
- Humans, Systematic Reviews as Topic, Surveys and Questionnaires, MEDLINE, Causality, Language
- Abstract
Objectives: We systematically evaluated causal language use in systematic reviews of observational studies and explored the relation between language use and the intent of the investigation., Study Design and Setting: We searched EMBASE, MEDLINE, and Epistemonikos. We randomly selected 199 reviews published in 2019, stratified in a 1:1 ratio by use and nonuse of the Grading of Recommendations Assessment, Development and Evaluation approach to rating quality of evidence., Results: Of 199 reviews of observational studies 56.8% had causal intent. Reviews with causal intent were more likely to investigate therapeutic clinical intervention (33.6% vs. 12.8%). Although 78.8% of those with causal intent used causal language in one or more sections of the title, abstract, or main text, only 4.4% consistently used causal language throughout the manuscript, and 21.2% did not use causal language at all. Of reviews without causal intent, 51.2% used causal language somewhere in the manuscript., Conclusion: Systematic reviews of observational studies sometimes do and sometimes do not have causal intent. Both those are inconsistent in causal language use and often use language inconsistent with the intent. Journal policies would better serve clarity of thinking and appropriateness of inferences by demanding authors clearly specify their intent and consistently use language consistent with that intent., (Copyright © 2022 Elsevier Inc. All rights reserved.)
- Published
- 2022
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9. Gut microbiota induces DNA methylation via SCFAs predisposing obesity-prone individuals to diabetes.
- Author
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Guo W, Zhang Z, Li L, Liang X, Wu Y, Wang X, Ma H, Cheng J, Zhang A, Tang P, Wang CZ, Wan JY, Yao H, and Yuan CS
- Subjects
- DNA Methylation, Fatty Acids, Volatile metabolism, Humans, Obesity genetics, Obesity metabolism, Propionates pharmacology, Tandem Mass Spectrometry, Diabetes Mellitus, Gastrointestinal Microbiome
- Abstract
Obesity-prone (OP) individuals have a significant predisposition to obesity and diabetes. Previously, we have found that OP individuals, despite being normal in weight and BMI, have already exhibited diabetes-related DNA methylation signatures. However, the underlying mechanisms remain obscure. Here we determined the effects of gut microbiota on DNA methylation and investigated the underlying mechanism from microbial-derived short-chain fatty acids (SCFAs). Diabetes-related DNA methylation loci were screened and validated in a new OP cohort. Moreover, the OP group was revealed to have distinct gut microbiota compositions, and fecal microbiota transplantation (FMT) demonstrated the role of gut microbiota in inducing diabetes-related DNA methylations and glucolipid disorders. UPLC-ESI-MS/MS analysis indicated a significantly lower level of total fecal SCFAs in the OP group. The gut microbiota from OP subjects yielded markedly decreased total SCFAs, while notably enriched propionate. Additionally, propionate was also identified by variable importance in projection (VIP) score as the most symbolic SCFAs of the OP group. Further cellular experiments verified that propionate could induce hypermethylation at locus cg26345888 and subsequently inhibit the expression of the target gene DAB1, which was crucially associated with clinical vitamin D deficiency and thus may affect the development and progression of diabetes. In conclusion, our study revealed that gut microbiota-derived propionate induces specific DNA methylation, thus predisposing OP individuals to diabetes. The findings partially illuminate the mechanisms of diabetes susceptibility in OP populations, implying gut microbiota and SCFAs may serve as promising targets both for clinical treatment and medication development of diabetes., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2022
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10. LRP5 Promotes Gastric Cancer via Activating Canonical Wnt/β-Catenin and Glycolysis Pathways.
- Author
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Nie X, Wang H, Wei X, Li L, Xue T, Fan L, Ma H, Xia Y, Wang YD, and Chen WD
- Subjects
- Animals, Carcinogenesis, Cell Line, Tumor, Cell Proliferation genetics, Glycolysis, Mice, Mice, Nude, beta Catenin metabolism, Low Density Lipoprotein Receptor-Related Protein-5 genetics, Stomach Neoplasms pathology, Wnt Signaling Pathway
- Abstract
The overactivation of canonical Wnt/β-catenin pathway is one of the main cascades for the initiation, progression, and recurrence of most human malignancies. As an indispensable coreceptor for the signaling transduction of the canonical Wnt/β-catenin pathway, LRP5 is up-regulated and exerts a carcinogenic role in most types of cancer. However, its expression level and role in gastric cancer (GC) has not been clearly elucidated. The current work showed that LRP5 was overexpressed in GC tissues and the expression of LRP5 was positively associated with the advanced clinical stages and poor prognosis. Ectopic expression of LRP5 enhanced the proliferation, invasiveness, and drug resistance of GC cells in vitro, and accelerated the tumor growth in nude mice, through activating the canonical Wnt/β-catenin signaling pathway and up-regulating aerobic glycolysis, thus increasing the energy supply for GC cells. Additionally, the expression of LRP5 and glycolysis-related genes showed an obviously positive correlation in GC tissues. By contrast, the exact opposite results were observed when the endogenous LRP5 was silenced in GC cells. Collectively, these results not only reveal the carcinogenic role of LRP5 during GC development through activating the canonical Wnt/β-catenin and glycolysis pathways, but also provide a valuable candidate for the diagnosis and treatment of human GC., (Copyright © 2022 American Society for Investigative Pathology. Published by Elsevier Inc. All rights reserved.)
- Published
- 2022
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11. Reconciling contrasting guideline recommendations on red and processed meat for health outcomes.
- Author
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Vernooij R, Guyatt GH, Zeraatkar D, Han MA, Valli C, El Dib R, Alonso-Coello P, Bala MM, and Johnston BC
- Subjects
- Humans, Risk Factors, Diet standards, Guidelines as Topic, Meat Products standards, Nutrition Policy, Recommended Dietary Allowances, Red Meat standards
- Published
- 2021
- Full Text
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12. Association of short-term fine particulate matter exposure with pulmonary function in populations at intermediate to high-risk of cardiovascular disease: A panel study in three Chinese cities.
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Ma H, Liu F, Yang X, Liu Q, Wang X, Xing X, Lin Z, Cao J, Li J, Huang K, Yan W, Liu T, Fan M, Chen S, Lu X, Gu D, and Huang J
- Subjects
- Adult, Air Pollutants analysis, Air Pollution analysis, Blood Pressure, Cardiovascular Diseases chemically induced, China, Cities, Environmental Exposure analysis, Forced Expiratory Volume, Humans, Male, Middle Aged, Particulate Matter analysis, Risk Factors, Vital Capacity, Air Pollutants adverse effects, Air Pollution adverse effects, Cardiovascular Diseases physiopathology, Environmental Exposure adverse effects, Lung drug effects, Particulate Matter adverse effects, Respiratory Function Tests
- Abstract
Background: Decline in pulmonary function contributes to increasing cardiovascular disease (CVD) risk. Although adverse effects of short-term exposure to fine particulate matter (PM
2.5 ) on pulmonary function have been recognized in healthy people or patients with respiratory disease, these results were not well illustrated among people with elevated CVD risk., Materials and Methods: A panel study was conducted in three Chinese cities with three repeated visits among populations at intermediate to high-risk of CVD, defined as treated hypertension patients or those with blood pressure ≥ 130/80 mmHg, who met any of the three conditions including abdominal obesity, dyslipidemia, and diabetes mellitus. Individualized PM2.5 exposure and pulmonary function were measured during each seasonal visit. Linear mixed-effect models were applied to analyze the associations of PM2.5 concentrations with pulmonary function indicators, including forced expiratory volume in 1 s (FEV1 ), FEV1 /forced vital capacity (FVC), maximal mid-expiratory flow (MMF), and peak expiratory flow (PEF)., Results: Short-term PM2.5 exposure was significantly associated with decreased pulmonary function and an increment of 10 μg/m3 in PM2.5 concentrations during lag 12-24 hour was associated with declines of 41.7 ml/s (95% confidence interval [CI]: 7.7-75.7), 0.35% (95% CI: 0.01, 0.69), and 20.9 ml/s (95% CI: 0.5-41.3) for PEF, FEV1 /FVC, and MMF, respectively. Results from stratified and sensitivity analyses were generally similar with the overall findings, while the adverse effects of PM2.5 on pulmonary functions were more pronounced in those who were physically inactive., Conclusions: This study first identified short-term exposure to PM2.5 was associated with impaired pulmonary function and physical activity might attenuate the adverse effects of PM2.5 among populations at intermediate to high-risk of CVD. These findings provide new robust evidence on health effects of air pollution and call for effective prevention measures among people at CVD risk., (Copyright © 2021. Published by Elsevier Inc.)- Published
- 2021
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13. Effects of soy protein isolate on gel properties and water holding capacity of low-salt pork myofibrillar protein under high pressure processing.
- Author
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Li YP, Kang ZL, Sukmanov V, and Ma HJ
- Subjects
- Animals, Color, Cooking, Food Handling methods, Gels, Myofibrils chemistry, Pressure, Rheology, Swine, Water, Meat Products analysis, Meat Proteins analysis, Soybean Proteins
- Abstract
This paper studies the effects of soy protein isolate (SPI; 0, 2% and 4%; Weight/Weight) on texture, rheological property, sulfhydryl groups, and the water distribution state of low-salt (1% NaCl) pork myofibrillar protein systems under high pressure processing (HPP, 200 MPa, 10 min). The L
⁎ value, cooking yield, hardness, total and reactive sulfhydryl, surface hydrophobicity, and the G' value at 80 °C of pork myofibrillar protein increased significantly (P < 0.05) when SPI was added; however, the springiness, cohesiveness, and chewiness of gels with 4% SPI were lower than of gels with 2% SPI. The rheological findings indicated that the thermal stability of the myofibrillar protein increased when SPI was added. The initial relaxation time of T2b , T21 , and T22 decreased when SPI increased; meanwhile, the peak ratio of P21 increased significantly (P < 0.05), implying that water had lower mobility. Overall, the 2% SPI could enhance gel characteristics and water-holding capacity of pork myofibrillar protein under 200 MPa., (Copyright © 2021 Elsevier Ltd. All rights reserved.)- Published
- 2021
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14. Effect of sodium alginate on physical-chemical, protein conformation and sensory of low-fat frankfurters.
- Author
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Kang ZL, Wang TT, Li YP, Li K, and Ma HJ
- Subjects
- Animals, Color, Cooking, Dietary Fats, Emulsions chemistry, Hardness, Humans, Swine, Alginates, Meat Products analysis, Protein Conformation
- Abstract
In order to study the potential of replacing pork back-fat by sodium alginate solution (sodium alginate/water = 1/29, W/W), the physico-chemical, protein conformation and sensory of frankfurters made with various amounts of pork back-fat and sodium alginate were evaluated. With the increase of sodium alginate solute, the fat and energy was decreased significantly (P < .05). Compared with the control, the cooking yield, emulsion stability, L
⁎ and ΔE values were not different significantly (P > .05) when pork back-fat was replaced by 25% and 50%, meanwhile, a decrease of α-helix content accompanied by an increase of β-sheet, β-turn and random coil content, more hydrophobic and tyrosine residues became exposed, those implied the hardness was increased. The texture, juiciness and overall acceptance scores were decreased significantly (P < .05) when pork back-fat was replaced beyond 50%. From the above, the use of sodium alginate solution instead of pork back-fat could transform protein structure and lowered fat and energy of frankfurters., Competing Interests: Declaration of Competing Interest The authors (Zhuang-Li Kang, Teng-teng Wang, Yan-ping Li, Ke Li, Han-jun Ma) declare that there are no financial interests or personal relationships that will influence the work reported in this paper., (Copyright © 2020 Elsevier Ltd. All rights reserved.)- Published
- 2020
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15. Morphophysiological responses of detached and adhered biofilms of Pseudomonas fluorescens to acidic electrolyzed water.
- Author
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Cai LL, Hu HJ, Lu Q, Wang HH, Xu XL, Zhou GH, Kang ZL, and Ma HJ
- Subjects
- Acids chemistry, Cell Membrane Permeability, Colony Count, Microbial, Disinfection methods, Membrane Potentials drug effects, Microscopy, Electron, Scanning, Pseudomonas fluorescens physiology, Water chemistry, Biofilms drug effects, Disinfectants pharmacology, Electrolysis, Pseudomonas fluorescens drug effects, Water pharmacology
- Abstract
Pseudomonas spp. have emerged as the main spoilage bacteria, with many strains easily forming biofilms on food-contact surfaces and causing cross-contamination. The efficacy of disinfectants against bacteria is usually tested with planktonic cells; however, the disinfection tolerance of biofilms, especially detached biofilms, remains unknown. Here, we investigated the tolerance responses of detached and adhered biofilms of Pseudomonas fluorescens to acidic electrolyzed water (AEW) by determining tolerance responses by plate counting, comparing them using a Weibull model, and verifying changes in bacterial morphology by scanning electron microscopy. The experimental data and the responses calculated using Weibull a (scale) and b (shape) parameters agreed well (R
2 values: 0.974-0.999), and we found that AEW exhibited effective antimicrobial activity against P. fluorescens, with adhered biofilms were more resistant than detached biofilms and planktonic cells. Additionally, AEW increased the bacterial membrane permeability and decreased the membrane potential, intracellular ATP concentrations, and intracellular pH while also triggering the disruption of extracellular polymeric substances. These results demonstrated that the morphophysiological responses of detached and adhered biofilms differed significantly and provided information on disinfectant-resistance strategies potentially beneficial to the development of novel disinfection approaches., (Copyright © 2019 Elsevier Ltd. All rights reserved.)- Published
- 2019
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16. A ratiometric fluorescence probe based on a novel recognition mechanism for monitoring endogenous hypochlorite in living cells.
- Author
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Yan YH, Ma HL, Miao JY, Zhao BX, and Lin ZM
- Subjects
- Animals, Mice, Molecular Structure, RAW 264.7 Cells, Fluorescent Dyes chemistry, Hypochlorous Acid analysis, Optical Imaging
- Abstract
A ratiometric fluorescence probe (named ZOC) for the fast detection of HClO/ClO
- was constructed by coumarin (donor) and pyridinium (acceptor) based on Forster resonance energy transfer (FRET) and intramolecular charge transfer (ICT) platform. ZOC possessed red emission signal (610 nm), large Stocks shift (190 nm), high energy transfer efficiency (95.3%), high selectivity and sensitivity, low detection limit (25 nM), wider detection range (from 25 nM to 30 μM), rapid response (within 13 S), and good biocompatibility. It was very interesting that the recognition mechanism involved a new organic reaction in which olefin double bond reacted first with HClO/ClO- regioselectively, followed by cyclization. ZOC was successfully used to the real time detection of endogenous HClO/ClO- in RAW 264.7 cells., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2019
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17. A novel lipid droplets-targeting ratiometric fluorescence probe for hypochlorous acid in living cells.
- Author
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Wu WL, Ma HL, Xi LL, Huang MF, Wang KM, Miao JY, and Zhao BX
- Subjects
- Animals, Cell Survival, Coumarins chemistry, Fluorescent Dyes chemistry, Lipid Droplets chemistry, Mice, Optical Imaging, RAW 264.7 Cells, Fluorescent Dyes metabolism, Hypochlorous Acid metabolism, Lipid Droplets metabolism
- Abstract
Lipid droplets were found to be involved in many organism activities. Here, a lipid droplets-targeted near-infrared fluorescence probe (named XHZ) for ratiometric detection of endogenous hypochlorous acid/hypochlorite (HClO/ClO
- ) in living cells was developed, which was constructed by a coumarin moiety and a malononitrile derivative. XHZ could detect HClO/ClO- with high selectivity and sensitivity in a ratiometric manner based on FRET (Förster Resonance Energy Transfer) mechanism. The two well-resolved emission (470/672 nm) bands could ensure accurate detection of HClO/ClO- in vitro as well as in vivo. XHZ was successfully used for ratiometric fluorescence imaging of exogenous and endogenous HClO/ClO- in RAW264.7 cells. A good linear relationship between the fluorescence intensity ratios of the two emissions and HClO/ClO- concentrations from 0 to 40 μM was obtained. Importantly, XHZ could localize mainly in lipid droplets of RAW264.7 cells. To the best of our knowledge, XHZ is the first lipid droplets-targeted ratiometric fluorescence probe for HClO/ClO- ., (Copyright © 2018 Elsevier B.V. All rights reserved.)- Published
- 2019
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18. Cutaneous Amyloidosis Presenting as Bilateral Nodular Masses on the Eyelids and Cheeks.
- Author
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Ma H, Lu C, and Lai W
- Subjects
- Amyloidosis, Familial diagnosis, Biopsy, Diagnosis, Differential, Eyelid Diseases diagnosis, Humans, Male, Middle Aged, Skin Diseases, Genetic diagnosis, Amyloidosis, Familial complications, Cheek pathology, Eyelid Diseases etiology, Eyelids pathology, Skin Diseases, Genetic complications
- Published
- 2018
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19. Structure-Guided Combinatorial Engineering Facilitates Affinity and Specificity Optimization of Anti-CD81 Antibodies.
- Author
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Nelson B, Adams J, Kuglstatter A, Li Z, Harris SF, Liu Y, Bohini S, Ma H, Klumpp K, Gao J, and Sidhu SS
- Subjects
- Antibodies, Neutralizing chemistry, Antibodies, Neutralizing pharmacology, Antibody Specificity, Binding Sites, Antibody, Cell Line, Hep G2 Cells, Hepacivirus drug effects, Hepacivirus immunology, Hepatitis C Antibodies pharmacology, Humans, Models, Molecular, Peptide Library, Protein Conformation, Single-Chain Antibodies pharmacology, Structure-Activity Relationship, Tetraspanin 28 chemistry, Virus Internalization drug effects, Hepacivirus physiology, Hepatitis C immunology, Hepatitis C Antibodies chemistry, Single-Chain Antibodies chemistry, Tetraspanin 28 immunology
- Abstract
Hepatitis C viral infection is the major cause of chronic hepatitis that affects as many as 71 million people worldwide. Rather than target the rapidly shifting viruses and their numerous serotypes, four independent antibodies were made to target the host antigen CD81 and were shown to block hepatitis C viral entry. The single-chain variable fragment of each antibody was crystallized in complex with the CD81 large extracellular loop in order to guide affinity maturation of two distinct antibodies by phage display. Affinity maturation of antibodies using phage display has proven to be critical to therapeutic antibody development and typically involves modification of the paratope for increased affinity, improved specificity, enhanced stability or a combination of these traits. One antibody was engineered for increased affinity for human CD81 large extracellular loop that equated to increased efficacy, while the second antibody was engineered for cross-reactivity with cynomolgus CD81 to facilitate animal model testing. The use of structures to guide affinity maturation library design demonstrates the utility of combining structural analysis with phage display technologies., (Copyright © 2018 Elsevier Ltd. All rights reserved.)
- Published
- 2018
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20. 6-Shogaol enhances renal carcinoma Caki cells to TRAIL-induced apoptosis through reactive oxygen species-mediated cytochrome c release and down-regulation of c-FLIP(L) expression.
- Author
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Han MA, Woo SM, Min KJ, Kim S, Park JW, Kim DE, Kim SH, Choi YH, and Kwon TK
- Subjects
- Animals, Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic isolation & purification, Antineoplastic Agents, Phytogenic pharmacology, CASP8 and FADD-Like Apoptosis Regulating Protein genetics, CASP8 and FADD-Like Apoptosis Regulating Protein metabolism, Carcinoma, Renal Cell metabolism, Carcinoma, Renal Cell pathology, Catechols chemistry, Catechols isolation & purification, Cell Line, Tumor, Cell Proliferation drug effects, Dose-Response Relationship, Drug, Down-Regulation drug effects, Drug Screening Assays, Antitumor, Humans, Kidney Neoplasms metabolism, Kidney Neoplasms pathology, Mice, Structure-Activity Relationship, Apoptosis drug effects, CASP8 and FADD-Like Apoptosis Regulating Protein deficiency, Carcinoma, Renal Cell drug therapy, Catechols pharmacology, Cytochromes c metabolism, Kidney Neoplasms drug therapy, Reactive Oxygen Species metabolism, TNF-Related Apoptosis-Inducing Ligand metabolism
- Abstract
6-Shogaol, a potent bioactive compound in ginger (Zingiber officinale Roscoe), has been reported for anti-inflammatory and anti-cancer activity. In this study, we investigated the effect of 6-shogaol to enhance tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis. The combined treatment with 6-shogaol and TRAIL markedly induces apoptosis in various cancer cells (renal carcinoma Caki cells, breast carcinoma MDA-MB-231 cells and glioma U118MG cells), but not in normal mesangial cells and normal mouse kidney cells. 6-Shogaol reduced the mitochondrial membrane potential (MMP) and released cytochrome c from mitochondria to cytosol via Bax activation. Furthermore, we found that 6-shogaol induced down-regulation of c-FLIP(L) expression at the post-translational levels and the overexpression of c-FLIP(L) markedly inhibited 6-shogaol plus TRAIL-induced apoptosis. Moreover, 6-shogaol increased reactive oxygen species (ROS) production in Caki cells. Pretreatment with ROS scavengers attenuated 6-shogaol plus TRAIL-induced apoptosis through inhibition of MMP reduction and down-regulation of c-FLIP(L) expression. In addition, 6-gingerol, another phenolic alkanone isolated from ginger, did not enhance TRAIL-induced apoptosis and down-regulate c-FLIP(L) expression. Taken together, our results demonstrated that 6-shogaol enhances TRAIL-mediated apoptosis in renal carcinoma Caki cells via ROS-mediated cytochrome c release and down-regulation of c-FLIP(L) expression., (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
21. Immune cell responses are not required to induce substantial hepatitis B virus antigen decline during pegylated interferon-alpha administration.
- Author
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Allweiss L, Volz T, Lütgehetmann M, Giersch K, Bornscheuer T, Lohse AW, Petersen J, Ma H, Klumpp K, Fletcher SP, and Dandri M
- Subjects
- Animals, DNA, Viral blood, Guanine administration & dosage, Guanine analogs & derivatives, Hepatitis B, Chronic immunology, Hepatitis B, Chronic virology, Hepatocytes virology, Humans, Interferon alpha-2, Mice, Recombinant Proteins administration & dosage, Antiviral Agents administration & dosage, Hepatitis B Surface Antigens blood, Hepatitis B, Chronic drug therapy, Interferon-alpha administration & dosage, Polyethylene Glycols administration & dosage
- Abstract
Background & Aims: Pegylated interferon-alpha (PegIFNα) remains an attractive treatment option for chronic hepatitis B virus (HBV) infection because it induces higher rates of antigen loss and seroconversion than treatment with polymerase inhibitors. Although early HBsAg decline is recognised as the best predictor of sustained response to IFN-based therapy, it is unclear whether immune cell functions are required to induce significant antigenemia reduction in the first weeks of treatment. Aim of the study was to investigate whether PegIFNα can induce sustained human hepatocyte responsiveness and substantial loss of circulating and intrahepatic viral antigen loads in a system lacking immune cell functions., Methods: HBV-infected humanized uPA/SCID mice received either PegIFNα, entecavir (ETV), or both agents in combination. Serological and intrahepatic changes were determined by qRT-PCR and immunohistochemistry and compared to untreated mice., Results: After 4 weeks of treatment, median viremia reduction was greater in mice treated with ETV (either with or without PegIFNα) than with PegIFNα. In contrast, levels of circulating HBeAg, HBsAg, and intrahepatic HBcAg were significantly reduced (p = 0.03) only in mice receiving PegIFNα alone or in combination, as compared to mice receiving ETV monotherapy. Progressive antigen reduction was also demonstrated in mice receiving PegIFNα for 12 weeks (HBeAg = Δ1log; HBsAg = Δ1.4log; p < 0.0001). Notably, repeated administrations of the longer-active PegIFNα could breach the impairment of HBV-infected hepatocyte responsiveness and induce sustained enhancement of human interferon stimulated genes (ISG)., Conclusions: The antiviral effects of PegIFNα exerted on the human hepatocytes can induce sustained responsiveness and trigger substantial HBV antigen decline without claiming the involvement of immune cell responses., (Copyright © 2013 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
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22. Impact of subclinical hypothyroidism on the development of non-alcoholic fatty liver disease: a prospective case-control study.
- Author
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Xu L, Ma H, Miao M, and Li Y
- Subjects
- Female, Humans, Male, Fatty Liver epidemiology, Hypothyroidism epidemiology, Metabolic Syndrome epidemiology
- Published
- 2012
- Full Text
- View/download PDF
23. Association between levels of physical activity and poor self-rated health in Korean adults: The Third Korea National Health and Nutrition Examination Survey (KNHANES), 2005.
- Author
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Han MA, Kim KS, Park J, Kang MG, and Ryu SY
- Subjects
- Adult, Aged, Chi-Square Distribution, Chronic Disease, Cross-Sectional Studies, Female, Health Surveys, Humans, Interviews as Topic, Korea, Logistic Models, Male, Middle Aged, Odds Ratio, Sampling Studies, Socioeconomic Factors, Young Adult, Exercise, Health Behavior, Health Status
- Abstract
Objective: To investigate the association between level of physical activity and poor self-rated health in Korean adults., Study Design: A cross-sectional study was conducted on 7,800 adults aged > or =19 years who completed the Health Interview and the Health Behavior Surveys, issued during the Third National Health and Nutrition Examination Survey (2005)., Methods: The association between self-rated health and physical activity was tested using Chi-squared test. Multiple logistic regression analysis was used to calculate the odds ratios of poor self-rated health for different levels of physical activity after adjusting for physical and chronic medical conditions., Results: The prevalence of poor self-rated health was significantly lower as the level of physical activity increased, and odds ratios for poor self-rated health were significantly lower for higher levels of physical activity after adjusting for age, marital status, educational status, smoking, alcohol consumption, and the number of physical impairments and chronic medical conditions. The association was similar in different age groups and among healthy respondents and respondents with physical impairments or chronic medical conditions., Conclusions: Physical activity was positively associated with self-rated health in Koreans. The independent association between a lower level of physical activity and poor self-rated health supports public health programmes that encourage regular physical activity.
- Published
- 2009
- Full Text
- View/download PDF
24. High pressure/thermal treatment effects on the texture of beef muscle.
- Author
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Ma HJ and Ledward DA
- Abstract
The effects of high pressure (to 800 MPa) applied at different temperatures (20-70 °C) for 20 min on beef post-rigor longissimus dorsi texture were studied. Texture profile analysis showed that when heated at ambient pressure there was the expected increase in hardness with increasing temperature and when pressure was applied at room temperature there was again the expected increase in hardness with increasing pressure. Similar results to those found at ambient temperature were found when pressure was applied at 40 °C. However, at higher temperatures, 60 and 70 °C it was found that pressures of 200 MPa caused large and significant decreases in hardness. The results found for hardness were mirrored by those for gumminess and chewiness. To further understand the changes in texture observed, intact beef longissimus dorsi samples and extracted myofibrils were both subjected to differential scanning calorimetry after being subjected to the same pressure/temperature regimes. As expected collagen was reasonably inert to pressure and only at temperatures of 60-70 °C was it denatured/unfolded. However, myosin was relatively easily unfolded by both pressure and temperature and when pressure denatured a new and modified structure was formed of low thermal stability. Although this new structure had low thermal stability at ambient pressure it still formed in both the meat and myofibrils when pressure was applied at 60 °C. It seems unlikely that structurally induced changes can be a major cause of the significant loss of hardness observed when beef is treated at high temperature (60-70 °C) and 200 MPa and it is suggested that accelerated proteolysis under these conditions is the major cause.
- Published
- 2004
- Full Text
- View/download PDF
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