158 results on '"T, Kanda"'
Search Results
2. Giant magnetoresistance and structural properties in NiFeCo/Cu multilayers
- Author
-
K. Meguro, T. Kanda, M. Jimbo, S. Goto, S. Tkunashima, and Susumu Uchiyama
- Subjects
Materials science ,Condensed matter physics ,Magnetoresistance ,Peak intensity ,Analytical chemistry ,Giant magnetoresistance ,Sputter deposition ,Layer thickness ,Antiferromagnetic coupling - Abstract
Giant magnetoresistance effect and structural properties of NiFeCo/Cu multilayers deposited by an rf magnetron sputtering method on glass substrates with Fe, NiFeCo, Cr and Zr buffer layers have been investigated. For all the buffer layers, the multilayers showed magnetoresistance which oscillated as a function of Cu layer thickness. The Fe and Cr buffered multilayers showed stronger antiferromagnetic coupling than the NiFeCo and Zr buffered ones. All the multilayers except for the Fe buffered ones exhibited more or less (111) preferred orientation. The Zr buffered multilayers showed especially strong orientation, where the X-ray (111) peak intensity was 20 times as large as for the NiFeCo, Cr or nonbuffered multilayers. more...
- Published
- 1994
- Full Text
- View/download PDF
Catalog
3. GLP-1 receptor signaling restores aquaporin 4 subcellular polarization in reactive astrocytes and promotes amyloid β clearance in a mouse model of Alzheimer's disease.
- Author
-
Sasaki K, Fujita H, Sato T, Kato S, Takahashi Y, Takeshita Y, Kanda T, Saito T, Saido TC, Hattori S, Hozumi Y, Yamada Y, and Waki H
- Subjects
- Animals, Mice, Liraglutide pharmacology, Mice, Transgenic, Mice, Inbred C57BL, Male, Humans, Peptide Fragments metabolism, Cell Polarity drug effects, Cerebral Cortex metabolism, Cerebral Cortex pathology, Cerebral Cortex drug effects, Aquaporin 4 metabolism, Aquaporin 4 genetics, Astrocytes metabolism, Astrocytes drug effects, Alzheimer Disease metabolism, Alzheimer Disease pathology, Amyloid beta-Peptides metabolism, Signal Transduction, Disease Models, Animal, Glucagon-Like Peptide-1 Receptor metabolism, Glucagon-Like Peptide-1 Receptor genetics
- Abstract
The physiological actions of a gut hormone, glucagon-like peptide-1 (GLP-1), in Alzheimer's disease (AD) brain remain poorly understood, although GLP-1 receptor (GLP-1R) expression in this organ has been shown in several experimental studies. Therefore, we explored whether the GLP-1R signaling promotes the clearance of amyloid β (Aβ) (1-42) which is a core pathological hallmark of AD, focusing on the water channel protein aquaporin 4 (AQP4) localized to astrocyte endfeet perivascular membranes in intact brain. First, we confirmed that Glp1r mRNA is predominantly expressed at perivascular site of astrocytes in normal mouse cerebral cortex through in situ hybridization analysis. Next, we observed that 20-week subcutaneous administration of a GLP-1R agonist (GLP-1RA) liraglutide significantly reduced Aβ (1-42) accumulation in the cerebral cortex and improved spatial working memory in an AD mouse model, App
NL-G-F/NL-G-F mice. Furthermore, our current data revealed that the 4-week liraglutide treatment relocalized subcellular AQP4 in morphologically injured reactive astrocytes of AppNL-G-F/NL-G-F mice to the cell surface perivascular site through PKA-mediated AQP4 phosphorylation. Such translocation of phosphorylated AQP4 to astrocyte cell surface following incubation with liraglutide was observed also in the present in vitro study using the cell line in which AQP4 cDNA was introduced into immortalized human astrocyte. These results suggest that enhanced intracerebral GLP-1R signaling following peripheral administration of GLP-1RA restores AQP4 subcellular polarization in reactive astrocytes and would promote Aβ excretion possibly through increasing AQP4-mediated intracerebral water flux in the brain in AD., Competing Interests: Declaration of competing interest K.S., H.F., S.K., Y.T., Y.Y., and H.W. received honoraria for lectures from Novo Nordisk. All other authors declared no competing interests., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.) more...- Published
- 2024
- Full Text
- View/download PDF
4. Peripheral nerve excitability abnormalities in Neuronal Intranuclear Inclusion Disease: Assessment with histopathological analysis.
- Author
-
Osaki Y, Nodera H, Sato R, Haji S, Fujita K, Miyamoto R, Muto K, Yamazaki H, Morino H, Kanda T, Murayama S, Kaji R, and Izumi Y
- Abstract
Objective: Neuronal Intranuclear Inclusion Disease (NIID) is a neurodegenerative disease affecting the central and peripheral nerves. We aimed to assess the pathophysiological features of peripheral nerve dysfunction in NIID., Methods: We observed six unrelated NIID patients through clinical records, nerve conduction studies, and multiple measures of motor nerve excitability. Additionally, we reviewed one NIID patientt who underwent a nerve biopsy. Control measures were obtained from 22 age-matched normal subjects., Results: The NIID patients exhibited mild conduction slowing and distinct nerve excitability abnormalities, including a significant decrease in excitability through hyperpolarizing threshold electrotonus (TE) and increased overshoots in both depolarizing and hyperpolarizing conditions. Histopathology revealed thinly myelinated fibers and axonal degeneration. Mathematical modeling suggested that reduced leak conductance was the key factor contributing to the observed excitability changes., Conclusions: The findings indicate that NIID involves a complex interplay of axonal degeneration and myelin dysfunction, leading to unique peripheral nerve excitability changes. These results provide new insights into the pathophysiology of NIID., Significance: Nerve excitability testing offers insight into particular axonal excitability abnormalities especially combined with histopathologic studies., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 International Federation of Clinical Neurophysiology. Published by Elsevier B.V. All rights reserved.) more...
- Published
- 2024
- Full Text
- View/download PDF
5. Methods and techniques to optimize energy delivery using the circular array pulsed field ablation catheter.
- Author
-
Mountantonakis S, Beccarino N, Abrams M, Sharma N, Skipitaris N, Bernstein N, Jia K, Bhasin K, Kanda T, Kordalis A, Tsiachris D, and Coleman K
- Abstract
Pulsed field ablation (PFA) is a novel method of cardiac ablation in which electrical fields are used to create microscopic pores in the cardiomyocyte cell membrane, resulting in cell death. Unlike traditional thermal radiofrequency and cryoablation technologies, PFA is cardiomyocyte preferential, reducing the risk of collateral damage to the esophagus and phrenic nerve. However, achieving durable lesions with PFA is dependent on the proximity to the tissue and presently approved systems do not provide contact force sensing. The PulseSelect PFA system is the first Food and Drug Administration-approved/CE-marked/Pharmaceuticals and Medical Devices Agency-approved system for PFA of atrial fibrillation; however, there is no consensus on workflow and best practices. We present here real-world experience from our centers in the United States, Europe, and Japan, and propose techniques and methods for incorporating fluoroscopy, electroanatomic mapping, and intracardiac echocardiography to assure optimal lesion delivery and predictability., Competing Interests: Disclosures The authors have no conflicts to disclose., (Copyright © 2024 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.) more...
- Published
- 2024
- Full Text
- View/download PDF
6. Cooperatively manual and endoscopic pancreatic stenting: A unique intraoperative procedure for postoperative pancreatic fistula after distal pancreatectomy.
- Author
-
Akabori H, Kanda T, Itoh A, and Mekata E
- Subjects
- Humans, Male, Pancreatic Neoplasms surgery, Middle Aged, Female, Aged, Pancreatic Fistula etiology, Pancreatic Fistula prevention & control, Pancreatectomy methods, Stents, Postoperative Complications etiology
- Abstract
Competing Interests: Declaration of competing interest There are no conflicts of interest to declare.
- Published
- 2024
- Full Text
- View/download PDF
7. Duration of atrial fibrillation persistence: Implications for recurrence risk after catheter ablation and efficacy of additional substrate ablation.
- Author
-
Matsunaga-Lee Y, Inoue K, Tanaka N, Masuda M, Watanabe T, Makino N, Egami Y, Oka T, Minamiguchi H, Miyoshi M, Okada M, Kanda T, Matsuda Y, Kawasaki M, Kawanami S, Ukita K, Kawamura A, Yasumoto K, Tsuda M, Okamoto N, Yano M, Nishino M, Sunaga A, Sotomi Y, Dohi T, Nakatani D, Hikoso S, and Sakata Y more...
- Subjects
- Humans, Male, Female, Middle Aged, Time Factors, Treatment Outcome, Aged, Risk Factors, Follow-Up Studies, Atrial Fibrillation surgery, Atrial Fibrillation physiopathology, Catheter Ablation methods, Recurrence, Pulmonary Veins surgery
- Abstract
Background: The optimal duration of atrial fibrillation (AF) persistence for predicting poor outcomes after catheter ablation of long-standing AF (LsAF) and the best ablation strategy for these patients remain unclear., Objective: We aimed to assess the impact of the duration of AF persistence on outcomes after catheter ablation of AF., Methods: We analyzed the Efficacy of Pulmonary Vein Isolation Alone in Patients with Persistent Atrial Fibrillation (EARNEST-PVI) trial data comparing pulmonary vein isolation (PVI) alone (PVI-alone) with additional linear ablation or defragmentation (PVI-plus) in persistent AF (PerAF). Patients who received catheter ablation by contact force-sensing catheter were enrolled in the study. In patients with LsAF, the optimal cutoff duration of AF persistence was evaluated. With use of the threshold, patients with LsAF were divided into 2 groups and compared with PerAF <1 year for arrhythmia-free survival after a 3-month blanking period., Results: The optimal cutoff duration was 2.4 years. Of 458 patients, arrhythmia-free survival rates for LsAF 1-2.4 years were comparable to those of PerAF (hazard ratio [HR], 1.01; 95% CI, 0.67-1.52). However, LsAF >2.4 years had a higher recurrence risk than PerAF (HR, 2.22; 95% CI, 1.42-3.47). In LsAF >2.4 years, the PVI-plus strategy showed advantages over the PVI-alone strategy (HR, 0.36; 95% CI, 0.14-0.89). However, the interaction effect between LsAF 1-2.4 years and LsAF >2.4 years did not reach statistical significance (P = .116)., Conclusion: Whereas LsAF 1-2.4 years has similar outcomes to those of PerAF, LsAF >2.4 years was linked to higher arrhythmia recurrence risks. For LsAF >2.4 years, the PVI-plus strategy showed a potential to be superior to the PVI-alone strategy., Competing Interests: Disclosures Drs Minamiguchi, Dohi, Nakatani, Hikoso, and Sakata have received grants from Medtronic, Johnson & Johnson, and Abbott during the conduct of the study. Other authors have nothing to disclose related to the submitted work. Conflict of interest outside the submitted work is found in a supplemental file., (Copyright © 2024 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.) more...
- Published
- 2024
- Full Text
- View/download PDF
8. Orthogeriatric co-management at a regional core hospital as a new multidisciplinary approach in Japanese hip fracture operation.
- Author
-
Higashikawa T, Shigemoto K, Moriyama M, Usuda D, Hangyou M, Inujima H, Nozaki K, Yamaguchi M, Usuda K, Iritani O, Morimoro S, Horii T, Nakahashi T, Matsumoto T, Hirohisa T, Takashima S, Kanda T, Okuro M, and Sawaguchi T more...
- Subjects
- Humans, Aged, Retrospective Studies, Japan epidemiology, Hospitals, Health Services for the Aged, Hip Fractures
- Abstract
Background: This study aimed to evaluate the effects of orthogeriatric co-management of hip fractures at a regional core hospital., Methods: This study included patients with proximal hip fracture. Patients were divided into two groups, conventional multidisciplinary group I including patients attending the hospital between April 2015 and March 2016 and orthogeriatric group II including patients attending the hospital between April 2016 and March 2017, which were compared retrospectively. In the control group, the conventional multidisciplinary team treated patients as whole-body controls. In the intervention group, the newly recruited geriatricians performed physical examinations, laboratory tests, radioactive imaging, and physiological tests. Furthermore, they consulted ward pharmacists, rigorously conducted positive polypharmacy interventions , and evaluated the type and number of mediated drugs on admission., Results: The number of medicated drugs significantly decreased from 6.03 ± 4.3 on admission to 5.50 ± 3.59 on discharge in group II, whereas group I did not show a significant decrease. Despite the more number of hospitalized patients in group II (166 patients) than in group I (126 patients), the recovery rate from postoperative urinary retention increased significantly from 57.8% (19/30) in group I to 84.3% (32/59) in group II (p = 0.049), while the incidence of aspiration pneumonia decreased from 7.1% (9/126) in group I to 2.49% (4/166) in group II (p = 0.08). The patients received six or more prescribed drugs on admission, and the number remained constant. However, the number of medicated drugs on discharge showed a marginally significant decrease from 6.03 ± 4.3 in group I to 5.50 ± 3.59 in group II (p < 0.05)., Conclusions: Compared to the conventional multidisciplinary group, the orthogeriatric team contributed to reducing the number of multi-effect drugs and perioperative complications without negatively affecting mortality despite the increased number of patients. The in-hospital mortality rate did not change between the groups. The orthogeriatric program succeeded in preventing and treating perioperative complications., Competing Interests: Declaration of competing interest None., (Copyright © 2022 The Japanese Orthopaedic Association. Published by Elsevier B.V. All rights reserved.) more...
- Published
- 2024
- Full Text
- View/download PDF
9. Pretilachlor-induced physiological, biochemical and morphological changes in Indian paddy field agroecosystem inhabited Anabaena doliolum.
- Author
-
Kanda T, Srivastava R, Yadav S, Singh N, Prajapati R, Singh PK, Yadav S, and Atri N
- Subjects
- Chlorophyll A pharmacology, Antioxidants metabolism, Cyanobacteria metabolism
- Abstract
Pretilachlor is a systemic, pre-emergence herbicide applied in the paddy fields to kill narrow and broadleaf weeds. The present study evaluates the toxicity of pretilachlor on the non-target diazotrophic free-living cyanobacterium Anabaena doliolum, commonly found in the paddy fields of eastern Uttar Pradesh (India) and used as a biofertilizer. A.doliolum was subjected to several doses (0, 2, 5, 7, 10, 20 and 40 μg/ml) of pretilachlor and its effects were examined in terms of alterations in cellular morphology, ultrastructure, physiology, and biochemical attributes. The treatment of pretilachlor decreased the growth, total pigment content and photosynthetic efficiency of the test organism in a dose-dependent manner. The decline in growth was observed on 20th day at 2, 5, 7, 10, 20 and 40 μg/ml of pretilachlor concentration by 4, 9, 26, 47, 71 and 92%, respectively. Furthermore, Chlorophyll a and phycocyanin levels were noticeably declined. As a result, the photosynthetic performance also registered a similar decline as measured by chlorophyll fluorescence. However, carotenoid content increased by 13%, 41% and 53% at 5, 10 and 20 μg/ml on 5th day reflecting its protective property. A marked increase in fluorescence intensity and malondialdehyde content by 2.65 and 2.45 folds at 10 and 20 μg/ml on 7th day was registered
. The enzymatic antioxidants (SOD and CAT) and a concurrent increase in glutathione reductase activity were registered (1.75 and 2.11-fold at 20 and 40 μg/ml on 5th day), indicating pretilachlor mediated ROS generation. Moreover, ultrastructural studies done by SEM and TEM revealed plasma membrane and thylakoid membrane damage and fragmentation. These findings have contributed to the broader comprehension of the stress responses triggered by pretilachlor in cyanobacteria. Moreover, they can aid in the evaluation of the detrimental impact of pretilachlor on A. doliolum, given their crucial function as a nitrogen contributor in paddy fields., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Inc.) more...- Published
- 2023
- Full Text
- View/download PDF
10. Ocular candidiasis in a tertiary hospital in Japan: A 10-year single-center retrospective study.
- Author
-
Sano T, Sasaki H, Maki Y, Nagaoka R, Hamamoto T, Sugaoka S, Kanda T, Takeuchi M, Kawana A, and Kimizuka Y
- Subjects
- Humans, Antifungal Agents pharmacology, Antifungal Agents therapeutic use, Retrospective Studies, Tertiary Care Centers, Japan epidemiology, Candida albicans, Candida glabrata, Candida parapsilosis, Microbial Sensitivity Tests, Candidemia drug therapy, Candidiasis drug therapy, Candidiasis epidemiology, Endophthalmitis drug therapy, Eye Infections, Fungal drug therapy, Eye Infections, Fungal epidemiology
- Abstract
Ocular candidiasis is a major complication of candidemia that is sometimes sight-threatening. Although prompt ophthalmologic consultation and antifungal medication have been emphasized, recent changes in the causative species and drug susceptibilities make the picture unclear. This study aimed to determine whether there are trends among patients with ocular candidiasis and included 80 patients with candidemia who underwent ophthalmological screening at our hospital between 2010 and 2020. Data on the clinical characteristics, comorbidities, biochemical test results, causative Candida species, treatment, outcomes, visual acuity, and antifungal susceptibility were collected and analyzed. Statistical analyses were performed by comparing two groups, namely, the ocular candidiasis (n = 29) and non-ocular candidiasis (n = 51) groups. In the ocular candidiasis group, there were significantly more cases of central venous catheter insertion (82.8%, p = 0.026) and Candida albicans candidemia (72.4%, p < 0.001). Regarding ocular involvement, the majority of patients were asymptomatic. Most cases improved with antifungal therapy, but one case underwent vitrectomy. Between 2016 and 2020, there was a diversification of species, with a decrease in Candida parapsilosis and the emergence of Candida glabrata and Candida tropicalis. Regarding drug susceptibility, the minimum inhibitory concentrations of echinocandin and 5-fluorocytosine against Candida albicans, Candida parapsilosis, and Candida glabrata were slightly increased. In conclusion, in addition to appropriately performing ophthalmologic examinations, it is beneficial to select antifungal agents according to the diversity of species and drug susceptibilities., Competing Interests: Declaration of competing interest The authors declare that they have no competing interests., (Copyright © 2023 Japanese Society of Chemotherapy, Japanese Association for Infectious Diseases, and Japanese Society for Infection Prevention and Control. Published by Elsevier Ltd. All rights reserved.) more...
- Published
- 2023
- Full Text
- View/download PDF
11. Impact of concomitant mitral regurgitation during transcatheter aortic valve replacement on 1-year survival outcomes.
- Author
-
Matsuura K, Kumamaru H, Kohsaka S, Kanda T, Yamashita D, Kitahara H, Shimamura K, Kobayashi Y, and Matsumiya G
- Subjects
- Humans, Treatment Outcome, Retrospective Studies, Aortic Valve surgery, Severity of Illness Index, Transcatheter Aortic Valve Replacement methods, Mitral Valve Insufficiency etiology, Aortic Valve Stenosis, Heart Valve Prosthesis Implantation methods, Heart Failure complications
- Abstract
Background: We investigated the influence of concomitant mitral regurgitation (MR) in patients undergoing transcatheter aortic valve implantation on the 1-year outcome using Japan Transcatheter Valve Therapy (J-TVT) registry data., Methods: The patients who underwent the transcatheter aortic valve implantation for aortic stenosis performed from August 2013 to December 2019 in Japan were included. History of previous valve surgery and dialysis patients were excluded. A total of 24,979 patients were included, and 1-year follow-up data were obtained from the registry (follow-up rate 98.5 %). Propensity-score matching, using multivariable logistic regression and 1:1 matching without replacement, was performed between the patients with grade 3-4 MR (MR 3-4 group) and those with grade 0-2 MR (MR 0-2 group). All-cause death and the composite outcome of death and/or heart failure events were compared., Results: After propensity score matching, 3920 cases (1960 cases each in MR 0-2 group and MR 3-4 group) were extracted. The procedure success rate was 96.4 % in MR 0-2 and 96.0 % in MR 3-4 group (p = 0.56) and the surgical conversion rate was 0.7 % in MR 0-2 group and 0.8 % in MR 3-4 group (p = 0.58). Cox regression model showed no statistical difference in 1-year survival rate between MR 0-2 group (89.4 %) and MR 3-4 group (89.6 %) (p = 0.80). However, freedom from 1-year death and/or heart failure event was lower in MR 3-4 (86.3 %) than in MR 0-2 group (88.9 %) (p = 0.01). This trend was also found in the subgroup of New York Heart Association (NYHA) class 1-2 but not in the subgroup of NYHA class 3-4., Conclusions: One-year survival rate was not different between groups but freedom from death and/or heart failure events was lower in patients with preoperative MR grade 3-4 than in patients with preoperative MR grade 0-2 after transcatheter aortic valve replacement., Competing Interests: Disclosures KM, TK, and GM report the affiliated department received scholarship funds from Otsuka Pharmaceutical. Co., Ltd., Century Medical Inc., Medtronic Japan Co., Ltd., Terumo Corp, Japan lifeline Co., Ltd. KM is a clinical proctor for Edwards Lifesciences. HK reports receiving consultation fees from Mitsubishi-Tanabe Pharma corporation, EPS Corporation, and speaker fees from Chugai Pharmaceutical Co., Ltd., and Johnson and Johnson. KK. SK reports an investigator-initiated grant from Novartis and Daiichi Sankyo and personal fees from Bristol-Myers Squibb. HK and SK are affiliated with the Department of Health Quality Assessment at the University of Tokyo, a social collaboration department supported by the National Clinical Database, Johnson & Johnson K.K., Nipro corporation, and Intuitive Surgical Sàrl., (Copyright © 2023 Elsevier Ltd. All rights reserved.) more...
- Published
- 2023
- Full Text
- View/download PDF
12. Effects of Intravenous Pimobendan on Cardiovascular Parameters in Healthy Sedated Cats.
- Author
-
Sugimoto K, Mochizuki Y, Akashi N, Kanda T, and Hoshi F
- Subjects
- Cats, Animals, Blood Pressure, Echocardiography veterinary, Cardiotonic Agents pharmacology, Cardiotonic Agents therapeutic use, Pyridazines pharmacology, Pyridazines therapeutic use
- Abstract
This study aimed to investigate the effects of intravenous pimobendan on cardiovascular function and to determine the appropriate dose for clinical usage in cats. Six purpose-bred cats received one of the following treatments: intravenous pimobendan at a single dose of 0.075 mg/kg (low dose [LD] group), 0.15 mg/kg (middle dose [MD] group), 0.3 mg/kg (high dose [HD] group), or saline at 0.1 mL/kg (placebo group). Echocardiography and blood pressure measurements were performed before and 5, 15, 30, 45, and 60 minute after drug administration for each treatment. In the MD and HD groups, the fractional shortening, peak systolic velocity, cardiac output, and heart rate increased significantly. There were no significant differences in blood pressure among the groups. Intravenous pimobendan at 0.15-0.3 mg/kg increased the fractional shortening, peak systolic velocity, cardiac output in healthy cats., (Copyright © 2023 Elsevier Inc. All rights reserved.) more...
- Published
- 2023
- Full Text
- View/download PDF
13. Miglustat, a glucosylceramide synthase inhibitor, mitigates liver fibrosis through TGF-β/Smad pathway suppression in hepatic stellate cells.
- Author
-
Iwanaga T, Chiba T, Nakamura M, Kaneko T, Ao J, Qiang N, Ma Y, Zhang J, Kogure T, Yumita S, Ishino T, Ogawa K, Kan M, Nakagawa M, Fujiwara K, Fujita N, Sakuma T, Kanzaki H, Koroki K, Kusakabe Y, Inoue M, Kobayashi K, Kanogawa N, Kiyono S, Kondo T, Nakagawa R, Ogasawara S, Nakamoto S, Muroyama R, Kato J, Kanda T, Maruyama H, Mimura N, Honda T, Murayama T, Nakamura H, and Kato N more...
- Subjects
- Animals, Humans, Mice, Carbon Tetrachloride pharmacology, Hepatic Stellate Cells metabolism, Liver metabolism, Liver Cirrhosis chemically induced, Liver Cirrhosis drug therapy, Liver Cirrhosis metabolism, Mice, Inbred C57BL, Signal Transduction, Smad Proteins metabolism, Transforming Growth Factor beta metabolism, Transforming Growth Factor beta1 metabolism
- Abstract
Transforming growth factor (TGF)-β/Smad pathway is implicated in the pathogenesis of liver fibrosis, a condition characterized by excessive deposition of extracellular matrix (ECM) proteins such as collagen in response to chronic inflammation. It has been reported that ceramide regulates collagen production through TGF-β/Smad pathway activation. In this study, we examined whether miglustat, an inhibitor of glucosylceramide synthase, can suppress liver fibrosis by reducing TGF-β/Smad pathway activity. Human hepatic stellate cells (HHSteCs) were cultured with TGF-β and multiple miglustat concentrations to examine dose-dependent effects on the expression levels of ECM-related genes and Smad proteins. To evaluate the efficacy of miglustat for fibrosis mitigation, C57BL/6 mice were treated with carbon tetrachloride (CCl
4 ) for 4 weeks to induce liver fibrosis, followed by combined CCl4 plus miglustat for a further 2 weeks. To examine if miglustat can also prevent fibrosis, mice were treated with CCl4 for 2 weeks, followed by CCl4 plus miglustat for 2 weeks. Miglustat dose-dependently downregulated expression of α-smooth muscle actin and ECM components in TGF-β-treated HHSteCs. Both phosphorylation and nuclear translocation of Smad2 and Smad3 were also suppressed by miglustat treatment. Sirius-Red staining and hydroxyproline assays of model mouse liver samples revealed that miglustat reduced fibrosis, an effect accompanied by decreased expression of ECM. Our findings suggest that miglustat can both prevent and reverse liver fibrosis by inhibiting TGF-β/Smad pathway., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Inc. All rights reserved.) more...- Published
- 2023
- Full Text
- View/download PDF
14. Intracellular glutathione levels affect the outcomes of verteporfin-mediated photodynamic therapy in esophageal cancer cells.
- Author
-
Edano M, Kanda T, Tarumoto R, Hamamoto W, Hasegawa T, Mae Y, Onoyama T, Takata T, Sugihara T, and Isomoto H
- Subjects
- Humans, Verteporfin pharmacology, Verteporfin therapeutic use, Photosensitizing Agents pharmacology, Photosensitizing Agents therapeutic use, Glutathione, Cell Line, Tumor, Photochemotherapy methods, Porphyrins pharmacology, Esophageal Neoplasms drug therapy
- Abstract
Photodynamic therapy (PDT) induces cancer cell death by generating reactive oxygen species (ROS). In this process, photosensitizers accumulate in cancer cells irradiated by laser light of a specific wavelength, leading to ROS generation. Verteporfin (VP), a second-generation photosensitizer, is used in PDT for age-related macular degeneration. However, the antitumor effects of VP-PDT remain poorly defined. This study investigated the antitumor effects of VP-PDT on esophageal cancer (EC) cell lines in vitro. Two types of EC cell lines, the KYSE30 cell line, derived from highly differentiated esophageal carcinoma, and the KYSE170 cell line, derived from moderately differentiated carcinoma, were used in this study. VP-PDT exerted effective anticancer effects in both cell lines. Our results revealed that the low-density lipoprotein receptor, albumin receptor, and heme carrier protein-1 in VP uptake were not involved in VP uptake. However, cells rich in intracellular glutathione were resistant to VP-PDT. Our study outcomes suggest that lowering intracellular glutathione via a glutathione synthesis inhibitor or sulfasalazine can increase the effectiveness of VP-PDT-mediated anticancer effects., (Copyright © 2022. Published by Elsevier B.V.) more...
- Published
- 2022
- Full Text
- View/download PDF
15. Proximal-tubule molecular relay from early Protein diaphanous homolog 1 to late Rho-associated protein kinase 1 regulates kidney function in obesity-induced kidney damage.
- Author
-
Ida-Naitoh M, Tokuyama H, Futatsugi K, Yasuda M, Adachi K, Kanda T, Tanabe Y, Wakino S, and Itoh H
- Subjects
- Animals, Cyclin-Dependent Kinases, Humans, Hypertrophy, Inflammation, Kidney Tubules, Proximal metabolism, Mice, Obesity complications, Albuminuria, rho-Associated Kinases metabolism
- Abstract
The small GTPase protein RhoA has two effectors, ROCK (Rho-associated protein kinase 1) and mDIA1 (protein diaphanous homolog 1), which cooperate reciprocally. However, temporal regulation of RhoA and its effectors in obesity-induced kidney damage remains unclear. Here, we investigated the role of RhoA activation in the proximal tubules at the early and late stages of obesity-induced kidney damage. In mice, a three-week high-fat-diet induced proximal tubule hypertrophy and damage without increased albuminuria, and RhoA/mDIA1 activation without ROCK activation. Conversely, a 12-week high-fat diet induced proximal tubule hypertrophy, proximal tubule damage, increased albuminuria, and RhoA/ROCK activation without mDIA1 elevation. Proximal tubule hypertrophy resulting from cell cycle arrest accompanied by downregulation of the multifunctional cyclin-dependent kinase inhibitor p27Kip1 was elicited by RhoA activation. Mice overexpressing proximal tubule-specific and dominant-negative RHOA display amelioration of high-fat diet-induced kidney hypertrophy, cell cycle abnormalities, inflammation, and renal impairment. In human proximal tubule cells, mechanical stretch mimicking hypertrophy activated ROCK, which triggered inflammation. In human kidney samples from normal individuals with a body mass index of about 25, proximal tubule cell size correlated with body mass index, proximal tubule cell damages, and mDIA1 expression. Thus, RhoA activation in proximal tubules is critical for the initiation and progression of obesity-induced kidney damage. Hence, the switch in the downstream RhoA effector in proximal tubule represents a transition from normal to pathogenic kidney adaptation and to body weight gain, leading to obesity-induced kidney damage., (Copyright © 2022. Published by Elsevier Inc.) more...
- Published
- 2022
- Full Text
- View/download PDF
16. Impacts of SNPs on adverse events and trough concentration of imatinib in patients with gastrointestinal stromal tumors.
- Author
-
Maekawa K, Yamamura M, Matsuki A, Ishikawa T, Hirai T, Yamaguchi Y, Saito Y, and Kanda T
- Subjects
- Genotype, Humans, Imatinib Mesylate adverse effects, Polymorphism, Single Nucleotide genetics, Antineoplastic Agents adverse effects, Gastrointestinal Stromal Tumors drug therapy, Gastrointestinal Stromal Tumors genetics
- Abstract
Although imatinib has dramatically improved the outcomes of patients with gastrointestinal stromal tumor (GIST), marked inter-individual differences in its efficacy and toxicity have been observed. Extensive pharmacogenetic studies in Caucasian and Asian populations have demonstrated that several genetic polymorphisms are involved in these differences; however, no studies have focused on Japanese patients with GIST. This study aimed to evaluate the impacts of genetic polymorphisms of drug metabolizing enzymes and transporters on the incidence of adverse events and trough plasma concentrations (C
trough s) of imatinib in Japanese patients with GIST. Of 35 candidate SNPs genotyped from 65 patients, ABCG2 421C>A was significantly associated with increased incidence rates of grade 2 or higher rash. When relationships between the genotypes and Ctrough s were examined in a subgroup of 38 patients from whom plasma was available, 5 SNPs were associated with significant trends toward increased or decreased dose-adjusted Ctrough s. Of them, SLCO1B3 334T>G and SLCO1A2 -1032G>A made significant contributions to the individual variability of Ctrough by multivariate regression analysis. Genetic variations in ABCG2, SLCO1B3, and SLCO1A2 may play important roles in the safety and pharmacokinetics of imatinib in Japanese patients with GIST, although a replication study is necessary for validation., Competing Interests: Declaration of competing interest None., (Copyright © 2021 The Japanese Society for the Study of Xenobiotics. Published by Elsevier Ltd. All rights reserved.) more...- Published
- 2022
- Full Text
- View/download PDF
17. Saphenous vein size as a surrogate marker for mortality of patients with chronic limb-threatening ischemia undergoing endovascular therapy.
- Author
-
Hata Y, Iida O, Takahara M, Asai M, Masuda M, Okamoto S, Ishihara T, Nanto K, Kanda T, Tsujumura T, Matsuda Y, Yanaka K, and Mano T
- Subjects
- Biomarkers, Humans, Ischemia diagnostic imaging, Limb Salvage, Retrospective Studies, Risk Factors, Saphenous Vein diagnostic imaging, Time Factors, Treatment Outcome, Vascular Patency, Endovascular Procedures, Peripheral Arterial Disease surgery
- Abstract
Background: The size of the autologous vein graft is an important determinant of prognosis in patients with chronic limb-threatening ischemia (CLTI) undergoing bypass surgery. However, it is unclear whether reconstruction using a small vein graft would directly affect prognosis, or a subgroup having small veins would be subject to a poor prognosis. If the latter is true, having small veins would work as a marker for a poor prognosis even in patients with CLTI undergoing endovascular therapy (EVT). Therefore, we investigated the association between the size of saphenous veins and prognosis in patients with CLTI undergoing EVT., Methods: This study included 209 consecutive patients with CLTI who primarily underwent EVT between January 2017 and December 2018. The diameter of the saphenous vein was measured at three locations (saphenous-femoral junction, knee, and ankle joint) per limb before EVT by ultrasonography. A small saphenous vein was defined as the mean diameter <3.0 mm., Results: All-cause mortality rate was significantly higher in patients with a small saphenous vein than in those with non-small one (46.3% versus 10.7% at 2 years, p<0.001). The multivariate Cox regression analysis revealed that a small saphenous vein was independently associated with an increased risk of all-cause mortality., Conclusions: Having a small saphenous vein was an independent marker for all-cause mortality in patients with CLTI undergoing EVT., (Copyright © 2021. Published by Elsevier Ltd.) more...
- Published
- 2021
- Full Text
- View/download PDF
18. Mycoplasma bovis May Travel Along the Eustachian Tube to Cause Meningitis in Japanese Black Cattle.
- Author
-
Suwanruengsri M, Uemura R, Izzati UZ, Kanda T, Fuke N, Yasuda M, Hirai T, and Yamaguchi R
- Subjects
- Animals, Cattle, Molecular Diagnostic Techniques, Nucleic Acid Amplification Techniques, Cattle Diseases microbiology, Eustachian Tube microbiology, Meningitis veterinary, Mycoplasma bovis
- Abstract
Mycoplasma bovis (M. bovis) is a common inhabitant of the upper and lower respiratory tracts of cattle and is considered to be the main aetiological agent of otitis media in calves. The eustachian tube appears to be the most common portal for pathogens to enter the middle ear. We investigated the transmission route of M. bovis causing otitis media that progressed to meningitis or meningoencephalitis in Japanese Black cattle. M. bovis was detected in 10 cases by a loop-mediated isothermal amplification method or by immunohistochemistry. One case of caseonecrotic granulomatous meningoencephalitis, one case of caseonecrotic granulomatous meningitis, one case of suppurative meningoencephalitis, eight cases of eustachitis, nine cases of tonsillitis and six cases of suppurative bronchopneumonia were identified by histopathological examination. M. bovis antigen was detected in the eustachian tubes of eight cases. In nine cases, M. bovis was also detected in tonsillar epithelial crypts and lumina, in intraluminal inflammatory cells and in the epithelial cells of minor salivary glands located around the eustachian tubes and tonsils. The results suggest that M. bovis can infect and colonize the tonsils and enter the eustachian tubes, causing otitis media, which, in cases of chronic infection, can progress to meningitis., (Copyright © 2021 Elsevier Ltd. All rights reserved.) more...
- Published
- 2021
- Full Text
- View/download PDF
19. Angioscopic assessments and clinical outcomes one year after polymer-free biolimus A9-coated coronary stent implantation.
- Author
-
Tsujimura T, Ishihara T, Okuno S, Iida O, Kurata N, Asai M, Masuda M, Okamoto S, Nanto K, Kanda T, Matsuda Y, Hata Y, and Mano T
- Subjects
- Absorbable Implants, Angioscopy, Humans, Polymers, Prosthesis Design, Sirolimus analogs & derivatives, Stents, Treatment Outcome, Coronary Artery Disease diagnostic imaging, Coronary Artery Disease surgery, Drug-Eluting Stents, Percutaneous Coronary Intervention adverse effects
- Abstract
Background: Polymer-free biolimus A9-coated coronary stent (DCS) has novel features which lead to the expectation of better arterial healing. However, comparisons of intravascular status between DCS and drug-eluting stents (DES), and robust real-word clinical assessments of DCS have been lacking to date., Methods: From September 2017 to September 2018, we evaluated the intra-vascular status of 74 DCS implanted in 55 lesions from 43 patients using coronary angioscopy (CAS) approximately one year after implantation from a cohort of 219 lesions in 158 patients. We set 239 second-generation durable-polymer DES (DP-DES) implanted in 211 lesions from 180 patients from a cohort of 2652 lesions in 1914 patients as the control. Angioscopic images were analyzed to determine (1) the dominant degree of neointimal coverage (NIC) over the stent; (2) the heterogeneity of NIC; (3) yellow color grade of the stented segment; and (4) the presence of intra-stent thrombus. The primary outcome was the incidence of thrombus and secondary outcomes were the other CAS findings, and the 1-year clinical outcomes which included target lesion revascularization (TLR) and major adverse cardiac events (MACE). To minimize inter-group differences in baseline characteristics, propensity score matching was performed for clinical outcomes., Results: Incidence of thrombus adhesion was similar in DCS and DP-DES groups (28.4% versus 22.6%, p=0.31). However, the dominant NIC grade was significantly higher in DCS (p<0.001), while NIC was more heterogeneous in DCS than in DP-DES (p=0.001). Maximum yellow color grade was similar (p=0.22). After propensity score matching, 202 lesion pairs from 146 patient pairs were retained for analysis. The cumulative incidence of TLR (4.6% versus 3.8%, p=0.38) and MACE (11.6% versus 11.7%, p=0.84) was similar for DCS and DP-DES., Conclusions: DCS showed thrombus adhesion and clinical outcomes at 1 year similar to DP-DES. DCS can thus be used with similar safety and efficacy as DP-DES., (Copyright © 2020 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved.) more...
- Published
- 2021
- Full Text
- View/download PDF
20. Acquisition of mesenchymal-like phenotypes and overproduction of angiogenic factors in lenvatinib-resistant hepatocellular carcinoma cells.
- Author
-
Ao J, Chiba T, Shibata S, Kurosugi A, Qiang N, Ma Y, Kan M, Iwanaga T, Sakuma T, Kanzaki H, Kanayama K, Kojima R, Kusakabe Y, Nakamura M, Saito T, Nakagawa R, Kondo T, Ogasawara S, Suzuki E, Muroyama R, Kato J, Mimura N, Kanda T, Maruyama H, and Kato N more...
- Subjects
- Carcinoma, Hepatocellular genetics, Cell Line, Tumor, Cell Movement drug effects, Cell Proliferation drug effects, Cytokines biosynthesis, Epithelial-Mesenchymal Transition drug effects, Gene Expression Regulation, Neoplastic drug effects, Human Umbilical Vein Endothelial Cells metabolism, Humans, Liver Neoplasms genetics, Neovascularization, Physiologic drug effects, Phenotype, RNA, Messenger genetics, RNA, Messenger metabolism, Angiogenesis Inducing Agents metabolism, Carcinoma, Hepatocellular pathology, Drug Resistance, Neoplasm drug effects, Liver Neoplasms pathology, Mesoderm pathology, Phenylurea Compounds pharmacology, Quinolines pharmacology
- Abstract
Lenvatinib is one of the first-line drugs for patients with advanced hepatocellular carcinoma (HCC) and widely used around the world. However, the mechanisms underlying resistance to lenvatinib remain unclear. In this study, we conducted characteristic analyses of lenvatinib-resistant HCC cells. Lenvatinib-resistant HCC cell lines were established by exposure to serially escalated doses of lenvatinib over 2 months. The biological characteristics of these cells were examined by in vitro assays. To investigate the cytokine profile of lenvatinib-resistant HCC cells, the supernatant derived from lenvatinib-resistant Huh7 cells was subjected to nitrocellulose membrane-based sandwich immunoassay. Both activation of the MAPK/MEK/ERK signaling pathway and upregulation of epithelial mesenchymal transition markers were observed in lenvatinib-resistant cells. Concordant with these findings, proliferation and invasion abilities were enhanced in these cells compared with control cells. Screening of a cytokine array spotted with 105 different antibodies to human cytokines enabled us to identify 16 upregulated cytokines in lenvatinib-resistant cells. Among them, 3 angiogenic cytokines: vascular endothelial growth factor (VEGF), platelet-derived growth factor-AA (PDGF-AA), and angiogenin, were increased significantly. Conditioned medium from lenvatinib-resistant cells accelerated tube formation of human umbilical vein cells. In conclusion, lenvatinib-resistant HCC cells were characterized by enhanced proliferation and invasion abilities. These findings might contribute to the establishment of new combination therapies with lenvatinib., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier Inc. All rights reserved.) more...
- Published
- 2021
- Full Text
- View/download PDF
21. Corrigendum to "Antimicrobial-Resistant Enterococcus faecium and Enterococcus faecalis Isolated From Healthy Thoroughbred Racehorses in Japan" [Journal of Equine Veterinary Science 94 (2020) 103232].
- Author
-
Sukmawinata E, Sato W, Uemura R, Kanda T, Kusano K, Kambayashi Y, Sato T, Ishikawa Y, Toya R, and Sueyoshi M
- Published
- 2021
- Full Text
- View/download PDF
22. Effects of uncomfortable care and histamine H2-antagonists on delirium in acute stroke: A propensity score analysis.
- Author
-
Nakamizo T, Kanda T, Kudo Y, Sugawara E, Hashimoto E, Okazaki A, Usuda M, Nagai T, Hara H, and Johkura K
- Subjects
- Histamine, Histamine H2 Antagonists, Humans, Intensive Care Units, Propensity Score, Prospective Studies, Delirium epidemiology, Delirium etiology, Stroke complications, Stroke drug therapy
- Abstract
Background and Purpose: Uncomfortable care and histamine H2 antagonist (H2A) are implicated in precipitating delirium. In acute stroke, however, the need for them depends on stroke severity, an established risk factor for delirium. So, it is unclear whether care or H2A itself is responsible for delirium. We aimed to evaluate their causal effects on delirium in acute stroke patients., Methods: This is a prospective cohort study on acute stroke patients admitted to a stroke care unit. Patients without stupor, coma, sedation, or delirium upon admission were enrolled. The treatment was H2A and five care modalities given during the first 24 h: restraint use, prohibited self-transfer, no oral feeding, indwelling catheters, and frequent nighttime care. The outcome was delirium within 5 days defined as Intensive Care Delirium Screening Checklist ≥4 points. We estimated the relative risk (RR) for delirium with regression models weighted by overlap weights using propensity scores estimated through logistic models incorporating known and potential confounders, including stroke severity., Results: Of the 387 participants, 188 were given at least one care modality and 130 were given H2A. A total of 42 developed delirium. Delirium was significantly associated with prohibited self-transfer (RR 1.7, 95% CI 1.0-3.0), frequent nighttime care (RR 2.1, 95% CI 1.2-3.7), and multiple care modalities (RR 2.4, 95% CI 1.3-4.4), while other care modalities and H2A were not., Conclusions: This study showed possible causal effects of uncomfortable care on delirium and suggests that minimizing it could prevent delirium in acute stroke., (Copyright © 2020 Elsevier B.V. All rights reserved.) more...
- Published
- 2021
- Full Text
- View/download PDF
23. Antimicrobial-Resistant Enterococcus faecium and Enterococcus faecalis Isolated From Healthy Thoroughbred Racehorses in Japan.
- Author
-
Sukmawinata E, Sato W, Uemura R, Kanda T, Kusano K, Kambayashi Y, Sato T, Ishikawa Y, Toya R, and Sueyoshi M
- Subjects
- Animals, Anti-Bacterial Agents pharmacology, Enterococcus faecalis, Horses, Japan epidemiology, Anti-Infective Agents, Enterococcus faecium
- Abstract
In this study, the occurrence of antimicrobial-resistant (AMR) enterococci was evaluated in Thoroughbred (TB) racehorses in Japan. Fecal samples were collected from 212 healthy TB racehorses at the Miho and Ritto Training Centers of the Japan Racing Association from March 2017 to August 2018. Isolation and identification were performed by enterococcus selective medium and confirmed to the species using MALDI-TOF MS. Enterococcus faecium and E. faecalis isolates were subjected to antimicrobial susceptibility test against 11 antimicrobials by minimum inhibitory concentration based on recommendation from Clinical and Laboratory Standards Institute guidelines. Among 583 enterococcus isolates, E. faecium and E. faecalis were identified for 48.2% (281/583) and 7.4% (43/583), respectively. One isolate that was representing E. faecium (153 isolates) and E. faecalis (31 isolates) from each sample was selected for antimicrobial susceptibility test. The highest rate of resistance for E. faecium isolates was observed against enrofloxacin (57.5%; 88/153), followed by streptomycin (32.0%; 49/153), kanamycin (18.3%; 28/153), gentamycin (5.9%; 9/153), erythromycin (5.9%; 9/153), and oxytetracycline (4.6%; 7/153). For E. faecium isolates, the highest resistance was observed against streptomycin (90.3%; 28/31), followed by kanamycin (41.9%; 13/31), gentamycin (29.0%; 9/31), lincomycin (9.7%; 3/31), oxytetracycline (6.5%; 2/31), erythromycin (6.5%; 2/31), tylosin (6.5%; 2/31), enrofloxacin (6.5%; 2/31), and chloramphenicol (3.2%; 1/31). The results indicated that enrofloxacin and aminoglycosides were highly resistant among tested antimicrobials. Continuous monitoring studies are useful to increase the awareness of the potential for AMR bacteria to arise from imprudent use of antimicrobials in TB racehorses in Japan., (Copyright © 2020 Elsevier Inc. All rights reserved.) more...
- Published
- 2020
- Full Text
- View/download PDF
24. Coinfection with Human Norovirus and Escherichia coli O25:H4 Harboring Two Chromosomal blaCTX-M-14 Genes in a Foodborne Norovirus Outbreak in Shizuoka Prefecture, Japan.
- Author
-
Nagaoka H, Hirai S, Morinushi H, Mizumoto S, Suzuki K, Shigemura H, Takahashi N, Suzuki F, Mochizuki M, Asanuma M, Maehata T, Ogawa A, Ohkoshi K, Sekizuka T, Ishioka T, Suzuki S, Kimura H, Kuroda M, Suzuki M, Murakami K, and Kanda T more...
- Subjects
- Anti-Bacterial Agents, Chromosomes, Disease Outbreaks, Escherichia coli genetics, Humans, Japan epidemiology, Microbial Sensitivity Tests, beta-Lactamases genetics, Coinfection, Escherichia coli Infections epidemiology, Norovirus genetics
- Abstract
Abstract: Hospital-acquired infections caused by extended-spectrum β-lactamase (ESBL)-producing Escherichia coli are a global problem. Healthy people can carry ESBL-producing E. coli in the intestines; thus, E. coli from healthy people can potentially cause hospital-acquired infections. Therefore, the transmission routes of ESBL-producing E. coli from healthy persons should be determined. A foodborne outbreak of human norovirus (HuNoV) GII occurred at a restaurant in Shizuoka, Japan, in 2018. E. coli O25:H4 was isolated from some of the HuNoV-infected customers. Pulsed-field gel electrophoresis showed that these E. coli O25:H4 strains originated from one clone. Because the only epidemiological link among the customers was eating food from this restaurant, the customers were concurrently infected with E. coli O25:H4 and HuNoV GII via the restaurant food. Whole genome analysis revealed that the E. coli O25:H4 strains possessed genes for regulating intracellular iron and expressing the flagellum and flagella. Extraintestinal pathogenic E. coli often express these genes on the chromosome. Additionally, the E. coli O25:H4 strains had plasmids harboring nine antimicrobial resistance genes. These strains harbored ESBL-encoding blaCTX-M-14 genes on two loci of the chromosome and had higher ESBL activity. Multilocus sequence typing and fimH subtyping revealed that the E. coli O25:H4 strains from the outbreak belonged to the subclonal group, ST131-fimH30R, which has been driving ESBL epidemics in Japan. Because the E. coli O25:H4 strains isolated in the outbreak belonged to a subclonal group spreading in Japan, foods contaminated with ESBL-producing E. coli might contribute to spreading these strains among healthy persons. The isolated E. coli O25:H4 strains produced ESBL and contained plasmids with multiple antimicrobial resistance genes, which may make it difficult to select antimicrobials for treating extraintestinal infections caused by these strains., (Copyright ©, International Association for Food Protection.) more...
- Published
- 2020
- Full Text
- View/download PDF
25. Antimicrobial susceptibility surveillance of bacterial isolates recovered in Japan from odontogenic infections in 2013.
- Author
-
Kaneko A, Matsumoto T, Iwabuchi H, Sato J, Wakamura T, Kiyota H, Tateda K, Hanaki H, Sakakibara N, Mizuno T, Miyajima H, Naito H, Takagi R, Kodama Y, Yamaguchi A, Akasiba R, Yamane N, Jinbu Y, Kusama M, Miyagi N, Kato R, Nakatogawa N, Izawa K, Tanzawa H, Kozu Y, Watanabe H, Matsumoto K, Shibahara T, Busujima Y, Takato T, Sakamoto H, Watanabe D, Kubota H, Sasaki J, Uematsu M, Sasaki M, Kaetsu A, Terasawa F, Yura Y, Iwai S, Morita S, Matsumoto K, Oonishi T, Komori T, Furudoi S, Fujibayashi J, Urade M, Kishimoto H, Yoshii T, Morihana T, Miyai D, Okamoto T, Kanda T, Okamoto K, Sakamoto A, Matsui Y, Miyake M, Sawai T, Ikebe T, and Hashimoto K more...
- Subjects
- Bacteria, Anaerobic, Clindamycin pharmacology, Clindamycin therapeutic use, Drug Resistance, Bacterial, Humans, Japan epidemiology, Microbial Sensitivity Tests, Penicillins, Anti-Bacterial Agents pharmacology, Anti-Bacterial Agents therapeutic use, Bacterial Infections drug therapy, Bacterial Infections epidemiology
- Abstract
We report on the findings of the first antimicrobial susceptibility surveillance study in Japan of isolates recovered from odontogenic infections. Of the 38 facilities where patients representing the 4 groups of odontogenic infections were seen, 102 samples were collected from cases of periodontitis (group 1), 6 samples from pericoronitis (group 2), 84 samples from jaw inflammation (group 3) and 54 samples from phlegmon of the jaw bone area (group 4) for a total of 246 samples. The positivity rates of bacterial growth on culture were 85.3%, 100%, 84% and 88.9%, respectively, for groups 1, 2, 3 and 4. Streptococcus spp. isolation rates according to odontogenic infection group were 22% (group 1), 17.7% (group 3) and 20.7% (group 4). Anaerobic isolation rates were 66.9% (group 1), 71.8% (group 3) and 68.2% (group 4). Drug susceptibility tests were performed on 726 strains excluding 121 strains that were undergrown. The breakdown of the strains subjected to testing was 186 Streptococcus spp., 179 anaerobic gram-positive cocci, 246 Prevotella spp., 27 Porphyromonas spp., and 88 Fusobacterium spp. The isolates were tested against 30 antimicrobial agents. Sensitivities to penicillins and cephems were good except for Prevotella spp. The low sensitivities of Prevotella spp is due to β-lactamase production. Prevotella strains resistant to macrolides, quinolones, and clindamycin were found. No strains resistant to carbapenems or penems were found among all strains tested. No anaerobic bacterial strain was resistant to metronidazole. Antimicrobial susceptibility testing performed on the S. anginosus group and anaerobic bacteria, which are the major pathogens associated with odontogenic infections, showed low MIC
90 values to the penicillins which are the first-line antimicrobial agents for odontogenic infections; however, for Prevotella spp., penicillins combined with β-lactamase inhibitor showed low MIC90 values., Competing Interests: Declaration of competing interest Tetsuya Matsumoto received speaker honoraria from MSD K.K., and Pfizer Japan Inc., Hiroshi Kiyota received scholarship donations from Taisho Toyama Pharmaceutical Co., Ltd., Fujifilm Toyama Chemical Co., Ltd., Daiichi Sankyo Co., Ltd., Astellas Pharma Inc., Taiho Pharmaceutical Co., Ltd., and Sanofi K.K., Kazuhiro Tateda received speaker honoraria from Pfizer Japan Inc., MSD K.K., Sumitomo Dainippon Pharma Co., Ltd., Meiji Seika Pharma Co., Ltd., and Taisho Toyama Pharmaceutical Co., Ltd., and grant support from Hitachi, Ltd., Nissui Pharmaceutical Co., Ltd., BD Japan Co., Ltd., Denka Co., Ltd., Kaneka Co., and Shimadzu Co., received scholarship donations from Fujifilm Toyama Chemical Co., Ltd., Meiji Seika Pharma Co., Ltd., Daiichi Sankyo Co., Ltd., Pfizer Japan Inc., Sumitomo Dainippon Pharma Co., Ltd., Shionogi & Co., Ltd., and Asahi Kasei Pharma Co., Hiromitsu Kishimoto received speaker honoraria from Daiichi Sankyo Co., Ltd., (Copyright © 2020 Japanese Society of Chemotherapy and The Japanese Association for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.) more...- Published
- 2020
- Full Text
- View/download PDF
26. Development of a clinical score, PANDA, to predict delirium in stroke care unit.
- Author
-
Nakamizo T, Kanda T, Kudo Y, Sugawara E, Hashimoto E, Okazaki A, Usuda M, Nagai T, Hara H, and Johkura K
- Subjects
- Critical Care, Humans, Intensive Care Units, Prospective Studies, Delirium diagnosis, Delirium epidemiology, Delirium etiology, Stroke complications, Stroke diagnosis
- Abstract
Background and Purpose: Delirium frequently complicates acute stroke and worsens outcomes. Because delirium is potentially preventable, predicting its occurrence is essential. Although several prediction scores have been proposed, nurses need to quickly predict delirium in stroke care units (SCUs). We aimed to develop a simple tool for this purpose by examining a comprehensive set of potential predictors., Methods: This is a prospective cohort study on acute stroke patients admitted to an SCU. Patients without stupor, coma, or delirium upon admission were eligible. Participants were followed for 5 days from admission. Delirium was defined as Intensive Care Delirium Screening Checklist ≥4 points. We examined 27 potential predictors, of which 13 predictors were used to developed a least absolute shrinkage and selection operator-penalized logistic regression model. Five variables with the largest coefficients were assigned one point each in the prediction score. The internal validation was performed by bootstrapping., Results: Delirium occurred in 42 of the 387 participants. The score consisted of prior delirium, alcohol, NIHSS ≥5, dementia, and auditory/visual impairment (PANDA). The apparent AUC was 0.84 (95% confidence interval [CI], 0.78-0.89), and the optimism-corrected AUC was 0.81 (95% CI, 0.73-0.88). With a cutoff of ≥2 points, sensitivity was 0.78 (95% CI, 0.65-0.90), and specificity was 0.74 (95% CI, 0.70-0.79)., Conclusions: PANDA score is simple and predicts delirium in an SCU satisfactorily., Competing Interests: Declaration of Competing Interest The authors have no conflicts of interest to disclose., (Copyright © 2020 Elsevier B.V. All rights reserved.) more...
- Published
- 2020
- Full Text
- View/download PDF
27. Effect of a heat and moisture exchanger on temperature and humidity of inhaled gas in isoflurane-anesthetized dogs.
- Author
-
Kanda T, Oishi Y, Kajiyama A, Shimizu Y, Maeta N, Furumoto K, and Furukawa T
- Subjects
- Animals, Female, Humidity, Male, Prospective Studies, Respiration, Artificial instrumentation, Temperature, Anesthesia, General veterinary, Anesthetics, Inhalation administration & dosage, Dogs physiology, Isoflurane administration & dosage, Oxygen Inhalation Therapy veterinary, Respiration, Artificial veterinary
- Abstract
Objective: To investigate the effects of a heat and moisture exchanger (HME) on the temperature and humidity of inhaled gas in isoflurane-anesthetized dogs., Study Design: Prospective, interventional study., Animals: A total of four experimental dogs and four client-owned dogs weighing 13.9 ± 7.4 kg (mean ± standard deviation)., Methods: The four experimental dogs were anesthetized on two occasions with and without an intact HME at least 1 week apart. The four client-owned dogs were anesthetized once only for a surgical procedure and assigned to the HME group or no-HME group in alternate order, resulting in six dogs for each group. All dogs were premedicated, anesthetized with propofol and intubated. The HME was connected to the endotracheal tube. Anesthesia was maintained with isoflurane. A digital thermo-hygrometer was placed between the endotracheal tube and HME. The temperature and relative humidity of the inhaled gas were measured every 5 minutes for 60 minutes and the absolute humidity was calculated at each time point., Results: The temperature and absolute humidity of the inhaled gas was significantly higher at 5-60 minutes after intubation in the HME group than in the no-HME group. Absolute humidity was maintained above 29 mg H
2 O L-1 in the HME group. No significant time-dependent effects on temperature, relative humidity or absolute humidity of the inhaled gas were observed., Conclusions and Clinical Relevance: The temperature and absolute humidity of the inhaled gas were higher when an HME was used during isoflurane anesthesia in dogs. The use of an HME may reduce the risk of dehydration and dysfunction of the airway mucosal epithelium., (Copyright © 2020 Association of Veterinary Anaesthetists and American College of Veterinary Anesthesia and Analgesia. Published by Elsevier Ltd. All rights reserved.) more...- Published
- 2020
- Full Text
- View/download PDF
28. Bovine Endocarditis Associated with Mycoplasma bovis.
- Author
-
Kanda T, Tanaka S, Suwanruengsri M, Sukmawinata E, Uemura R, Yamaguchi R, and Sueyoshi M
- Subjects
- Animals, Cattle, Cattle Diseases pathology, Endocarditis, Bacterial microbiology, Endocarditis, Bacterial pathology, Mycoplasma Infections microbiology, Mycoplasma Infections pathology, Cattle Diseases microbiology, Endocarditis, Bacterial veterinary, Mycoplasma Infections veterinary, Mycoplasma bovis isolation & purification
- Abstract
Mycoplasma bovis is a microorganism associated with pneumonia, mastitis, arthritis and otitis media of cattle; however, there are no reports of this organism causing bovine endocarditis. Five adult cattle with endocarditis characterized by caseated lesions (diameter 5-12 cm) of the endocardial surface of the left atrium, but without lesions in heart valves or affecting the right side of the heart, were identified in slaughterhouses in Japan. M. bovis was successfully isolated from the lesions and M. bovis antigen was detected immunohistochemically within the lesions. The results suggest that the lesions may have been associated with M. bovis alone. To our knowledge, this is the first demonstration of bovine endocarditis associated with M. bovis., (Copyright © 2019 Elsevier Ltd. All rights reserved.) more...
- Published
- 2019
- Full Text
- View/download PDF
29. Early- and middle-phase arterial repair following bioresorbable- and durable-polymer drug-eluting stent implantation: An angioscopic study.
- Author
-
Ishihara T, Tsujimura T, Okuno S, Iida O, Asai M, Masuda M, Okamoto S, Nanto K, Kanda T, Matsuda Y, and Mano T
- Subjects
- Aged, Coronary Artery Disease diagnosis, Coronary Vessels surgery, Female, Follow-Up Studies, Humans, Male, Prosthesis Design, Retrospective Studies, Time Factors, Treatment Outcome, Absorbable Implants, Angioscopy methods, Coronary Artery Disease surgery, Coronary Vessels pathology, Drug-Eluting Stents, Percutaneous Coronary Intervention methods, Polymers
- Abstract
Background: Bioresorbable-polymer drug-eluting stent (BP-DES) demonstrates comparable clinical outcomes compared to durable-polymer drug-eluting stent (DP-DES). However, early- and middle-phase arterial repair following deployment of BP-DES and DP-DES has not been elucidated to date., Methods: We extracted coronary angioscopy (CAS) findings covering early phase (4 ± 1 months) or middle phase (10 ± 2 months) between January 2010 and February 2018 from the database of Kansai Rosai Hospital. Neointimal coverage (NIC), yellow color intensity of the stented segment and incidence of thrombus adhesion were compared between BP-DES (Synergy or Ultimaster) and DP-DES (Promus or Resolute or Xience) in early (39 BP-DES of 33 lesions from 24 patients and 83 DP-DES of 74 lesions from 56 patients) and middle (198 BP-DES of 175 lesions from 135 patients and 204 DP-DES of 184 lesions from 149 patients) phases., Results: In early phase, while NIC was similar in both groups (P = 0.84), the incidence of thrombus adhesion was significantly higher in BP-DES than in DP-DES (67% versus 34%, P = 0.001) even though maximum yellow color was less intense in BP-DES (P = 0.004). In middle phase, while NIC was better in BP-DES (P < 0.001), thrombus adhesion (23% versus 22%, P = 0.81) and maximum yellow color (P = 0.72) were similar in both groups., Conclusions: Although NIC was similar in the early phase, the incidence of thrombus adhesion was significantly higher in BP-DES than in DP-DES. The incidence of thrombus adhesion reached similar values and NIC improved in BP-DES over that in DP-DES in the middle phase., (Copyright © 2019 Elsevier B.V. All rights reserved.) more...
- Published
- 2019
- Full Text
- View/download PDF
30. β-hydroxybutyrate attenuates renal ischemia-reperfusion injury through its anti-pyroptotic effects.
- Author
-
Tajima T, Yoshifuji A, Matsui A, Itoh T, Uchiyama K, Kanda T, Tokuyama H, Wakino S, and Itoh H
- Subjects
- 3-Hydroxybutyric Acid therapeutic use, Acetylation drug effects, Animals, Disease Models, Animal, Epigenesis, Genetic drug effects, Forkhead Box Protein O3 metabolism, Gene Expression Regulation drug effects, Histones metabolism, Humans, Infusions, Intravenous, Male, Mice, Promoter Regions, Genetic genetics, Pyroptosis genetics, Reperfusion Injury etiology, 3-Hydroxybutyric Acid pharmacology, Forkhead Box Protein O3 genetics, Kidney Tubules blood supply, Pyroptosis drug effects, Reperfusion Injury drug therapy
- Abstract
Ketone bodies including β-hydroxybutyrate (β-OHB) have been shown to protect against ischemic tissue injury when present at low concentrations. We evaluated the impact of β-OHB on renal ischemia/reperfusion injury (IRI). Mice were treated with a continuous infusion of β-OHB using an osmotic mini-pump before and after IRI. We also tested the effects of increasing endogenous serum β-OHB levels by fasting. Renal IRI was attenuated by β-OHB treatment compared to saline control, with similar results in the fasting condition. β-OHB treatment reduced the number of terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL)-positive cells and increased expression of forkhead transcription factor O3 (FOXO3), an upstream regulator of pyroptosis. Although β-OHB treatment did not impact markers of apoptosis, it decreased the expression of caspase-1 and proinflammatory cytokines, indicating that β-OHB blocked pyroptosis. In a human proximal tubular cell line exposed to hypoxia and reoxygenation, β-OHB reduced cell death in a FOXO3-dependent fashion. Histone acetylation was decreased in kidneys exposed to IRI and in proximal tubular cells exposed to hypoxia and reoxygenation, and this effect was ameliorated by β-OHB through the inactivation of histone deacetylases. In vitro, β-OHB treatment restored histone acetylation at the FOXO3 promoter. Consistent with epigenetic molecular effects, the renoprotective effects of β-OHB were still observed when the continuous infusion was stopped at the time of IRI. Thus, β-OHB attenuates renal IRI through anti-pyroptotic effects, likely mediated by an epigenetic effect on FOXO3 expression., (Copyright © 2019 International Society of Nephrology. Published by Elsevier Inc. All rights reserved.) more...
- Published
- 2019
- Full Text
- View/download PDF
31. Comparison of Clinical Outcomes between Endovascular Therapy with Self-Expandable Nitinol Stent and Femoral-Popliteal Bypass for Trans-Atlantic Inter-Society Consensus II C and D Femoropopliteal Lesions.
- Author
-
Okuno S, Iida O, Iida T, Takahara M, Yamaoka T, Kitano I, Asai M, Masuda M, Okamoto S, Ishihara T, Nanto K, Kanda T, Tsujimura T, Matsuda Y, and Mano T
- Subjects
- Aged, Aged, 80 and over, Endovascular Procedures adverse effects, Female, Femoral Artery diagnostic imaging, Femoral Artery physiopathology, Humans, Japan, Male, Peripheral Arterial Disease diagnostic imaging, Peripheral Arterial Disease physiopathology, Popliteal Artery diagnostic imaging, Popliteal Artery physiopathology, Prosthesis Design, Recurrence, Retrospective Studies, Risk Factors, Severity of Illness Index, Time Factors, Treatment Outcome, Vascular Grafting adverse effects, Vascular Patency, Alloys, Endovascular Procedures instrumentation, Femoral Artery surgery, Peripheral Arterial Disease surgery, Popliteal Artery surgery, Self Expandable Metallic Stents, Vascular Grafting methods
- Abstract
Background: Femoral-popliteal (F-P) bypass is the standard treatment for lower extremity peripheral artery disease with severe femoropopliteal (FP) artery lesions. However, in clinical settings, these patients are treated with endovascular therapy (EVT) because of frailty and difficulties with general anesthesia. We compared the clinical outcomes of F-P bypass and EVT for severe FP artery lesions and investigated the types of patients for whom EVT would be as appropriate as F-P bypass., Methods: This multicenter, retrospective study included 452 Trans-Atlantic Inter-Society Consensus (TASC) II C and D FP artery lesions in 352 patients. A total of 350 lesions in 260 patients (74 ± 9 years, 66% male, 60% claudication) were treated with EVT with nitinol self-expandable stents, and 102 lesions in 92 patients (71 ± 9 years, 75% male, 40% claudication) were treated with F-P bypass. The primary outcome measure was primary patency, and the influence of baseline characteristics on its association with the treatment strategy (EVT versus F-P bypass) was assessed using a Cox proportional hazards regression model., Results: Kaplan-Meier analysis indicated that the 3-year primary patency rate was significantly lower for EVT than F-P bypass (60% vs. 74%, P < 0.01). The body mass index (BMI) and C-reactive protein (CRP) levels significantly interacted with the treatment strategy for restenosis (P < 0.05). The adjusted hazard ratios of EVT versus F-P bypass for restenosis were 0.77 (P = 0.46) in cases with a low BMI (≤18 kg/m
2 ) or an elevated CRP level (≥1 mg/dL) and 3.35 (P < 0.01) in other cases. The 3-year primary patency rate was not significantly different between the EVT and F-P bypass groups in patients with BMI ≤ 18 kg/m2 or CRP ≥ 1 mg/dL (57% vs. 45%, P = 0.84)., Conclusions: In TASC II C and D lesions, EVT appears to yield patency comparable to that of F-P bypass in patients with a low BMI or an elevated CRP level, but lower patency in other patients., (Copyright © 2018 Elsevier Inc. All rights reserved.) more...- Published
- 2019
- Full Text
- View/download PDF
32. Centrifugal wave-front propagation speed for localizing the atrial tachycardia origin.
- Author
-
Kurata N, Masuda M, Asai M, Iida O, Okamoto S, Ishihara T, Nanto K, Kanda T, Tsujimura T, Matsuda Y, Okuno S, Ohashi T, Abe A, and Mano T
- Subjects
- Aged, Aged, 80 and over, Catheter Ablation methods, Female, Humans, Male, Middle Aged, Retrospective Studies, Tachycardia, Ectopic Atrial physiopathology, Tachycardia, Ectopic Atrial surgery, Electrocardiography methods, Imaging, Three-Dimensional methods, Tachycardia, Ectopic Atrial diagnostic imaging
- Abstract
Background: The earliest activation site (EAS) on a centrifugally-propagated atrial tachycardia (AT) map may represent the true AT origin (true-focal pattern), or the earliest site resulting from passive activation of AT originating from neighboring tissue (pseudo-focal pattern). We assessed the benefits of using the wave-front propagation speed to distinguish between the true- and the pseudo-focal pattern., Methods: AT mapping was performed using a novel ultra-high resolution mapping system with a 64-electrode mini-basket catheter. The true AT origin was defined as the site where radiofrequency application eliminated AT. The wave-front propagation speed was estimated from the area surrounded by the centrifugally-propagated wave front over a specific time interval., Results: Total of 46 centrifugally propagated AT maps from 34 patients were analyzed, including 18 true-focal and 28 pseudo-focal pattern. The area surrounded by the propagated wave front was significantly smaller for the true-focal pattern than for the pseudo-focal pattern, 1-20 msec after the earliest activation. The true-focal pattern was identified by the area 13 msec after the earliest activation, with the best cut-off area value of <4.5 cm
2 ., Conclusion: The presence or absence of a true origin of AT at the EAS on centrifugally-propagated AT maps can be distinguished using a wave-front propagation speed., (Copyright © 2018 Elsevier B.V. All rights reserved.) more...- Published
- 2019
- Full Text
- View/download PDF
33. Determination of serum carbohydrate-deficient transferrin by a nephelometric immunoassay for differential diagnosis of alcoholic and non-alcoholic liver diseases.
- Author
-
Nomura F, Kanda T, Seimiya M, Satoh M, Kageyama Y, Yamashita T, Yokosuka O, Kato N, and Maruyama K
- Subjects
- Biomarkers analysis, Biomarkers metabolism, Diagnosis, Differential, Female, Humans, Liver Diseases metabolism, Liver Function Tests, Male, Transferrin analysis, gamma-Glutamyltransferase metabolism, Immunoassay, Liver Diseases diagnosis, Nephelometry and Turbidimetry, Transferrin analogs & derivatives, gamma-Glutamyltransferase analysis
- Abstract
Background: Carbohydrate-deficient transferrin is a biological marker of excessive drinking. The aim of this study was to evaluate the diagnostic value of a direct nephelometric immunoassay for the differential diagnosis of alcoholic and non-alcoholic liver diseases in comparison with gamma glutamyl transferase., Methods: Serum samples were obtained from 305 subjects, including 122 patients with alcoholic and 102 cases with non-alcoholic liver diseases. Serum levels of carbohydrate-deficient transferrin were expressed as a percentage of total transferrin., Results: Serum % carbohydrate-deficient transferrin levels were significantly higher in patients with alcoholic than with non-alcoholic liver diseases. Carbohydrate-deficient transferrin had better specificity than gamma glutamyl transferase to differentiate between alcoholic and non-alcoholic liver diseases.There were 8 alcoholic liver disease patients with normal gamma glutamyl transferase levels, and carbohydrate-deficient transferrin was significantly elevated in 6 of them. On the other hand, there were 25 non-alcoholic liver disease patients with elevated gamma glutamyl transferase levels; their carbohydrate-deficient transferrin levels were within the reference intervals in all cases., Conclusion: This simple carbohydrate-deficient transferrin immunoassay is useful to detect so-called gamma glutamyl transferase non-responding drinkers and also to exclude the possible role of excessive drinking in apparently non-alcoholic liver diseases. A large-scale prospective study is needed to further confirm the diagnostic utility of carbohydrate-deficient transferrin., (Copyright © 2018 Elsevier B.V. All rights reserved.) more...
- Published
- 2018
- Full Text
- View/download PDF
34. Distal limb weakness phenotype of Guillain-Barré syndrome.
- Author
-
Koga M, Kishi M, Fukusako T, and Kanda T
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Antibodies cerebrospinal fluid, Campylobacter Infections physiopathology, Campylobacter jejuni pathogenicity, Child, Female, Gastrointestinal Tract physiopathology, Glycolipids immunology, Guillain-Barre Syndrome cerebrospinal fluid, Guillain-Barre Syndrome drug therapy, Humans, Immunoglobulins, Intravenous therapeutic use, Male, Middle Aged, Neural Conduction physiology, Phenotype, Retrospective Studies, Statistics, Nonparametric, Young Adult, Extremities physiopathology, Guillain-Barre Syndrome complications, Muscle Weakness etiology
- Abstract
Objective: Several regional variants of Guillain-Barré syndrome (GBS) have been proposed in western countries, but other variants remain unclear, especially among mildly disabled cases. The aim of this study was to identify unvalidated GBS phenotypes among Japanese patients with mild GBS., Methods: Retrospective study of a cohort of patients at a University Hospital in Japan., Results: Among 107 GBS patients, 25 (23%) were classified as having mild GBS (GBS disability scale ≤ 2 at nadir). A review of mild cases identified 8 patients (7.5% of all GBS and 32% of mild GBS) with an unusual phenotype, namely a distal limb weakness form of GBS (DL-GBS), which showed limited distribution of limb weakness within hands and feet with preserved strength in proximal limb muscles. The patients with DL-GBS were characterized by mild-to-moderate weakness in distal parts of limbs especially fingers, lacking or mild sensory disturbance at distal limbs, sometimes hyperreflexia at legs, and having prior Campylobacter jejuni enteritis. Among the patients with GBS after C. jejuni enteritis, DL-GBS patients were characterized by frequent detection of anti-GM1 antibodies without anti-GD1a antibodies, whereas the others were often positive for the two antibodies., Conclusions: DL-GBS is a distinct regional phenotype of GBS, which should be differentiated from cervical myelopathy. It can be generally categorized as a mild type of GBS after C. jejuni enteritis, which has characteristic pattern of anti-ganglioside autoantibodies., (Copyright © 2018 Elsevier B.V. All rights reserved.) more...
- Published
- 2018
- Full Text
- View/download PDF
35. Protoporphyrinogen oxidase is involved in the fluorescence intensity of 5-aminolevulinic acid-mediated laser-based photodynamic endoscopic diagnosis for early gastric cancer.
- Author
-
Kurumi H, Kanda T, Kawaguchi K, Yashima K, Koda H, Ogihara K, Matsushima K, Nakao K, Saito H, Fujiwara Y, Osaki M, Okada F, and Isomoto H
- Subjects
- Adenocarcinoma diagnosis, Adenocarcinoma pathology, Adult, Aged, Aged, 80 and over, Carcinoma, Signet Ring Cell diagnosis, Carcinoma, Signet Ring Cell pathology, Early Detection of Cancer, Female, Gastroscopy, Humans, Immunohistochemistry, Male, Middle Aged, Stomach Neoplasms pathology, Aminolevulinic Acid administration & dosage, Coproporphyrinogen Oxidase administration & dosage, Photosensitizing Agents administration & dosage, Protoporphyrinogen Oxidase administration & dosage, Stomach Neoplasms diagnosis
- Abstract
Background/aim: Laser-based photodynamic endoscopic diagnosis (LPDED) is a type of endoscopic diagnosis that uses the fluorescence caused by the photochemical reaction that occurs when a fluorescent substance is irradiated by a light of a specific wavelength. Although 5-aminolevulinic acid (5-ALA) can detect early gastric cancer (EGC) during LPDED, there is an unresolved issue of the differences in fluorescence intensity among histopathological types of gastric cancer. Thus, the aim of the present study was to assess whether protoporphyrinogen oxidase (PPOX), involved in the activation of protoporphyrin IX, can affect the fluorescence intensity in EGC., Methods: Thirty-three gastric tumor lesions in 30 patients were assessed by LPDED using a prototype endoscope equipped with a blue laser ray to cause excitation following oral 5-ALA administration. The tumors were then resected by endoscopic submucosal dissection or laparoscopic surgery. PPOX expression was examined immunohistochemically in the excised specimens. To explore the mechanisms of histopathological diversity in PPOX and coproporphyrinogen oxidase (CPOX) expression of EGC, immunohistochemical analysis was performed using 75 surgically resected specimens of diverse EGCs., Results: Among the 33 lesions, 26 tumors were detectable by LPDED, whereas seven were undetectable. Between the LPDED-positive and negative groups, there was a significant difference in histopathology. The expression of PPOX was higher in tubular adenocarcinoma (tub) than in signet-ring cell carcinoma (sig). There were significant differences in PPOX and CPOX expression scores of the surgically resected specimens among tub, poorly differentiated adenocarcinoma (por), and sig., Conclusion: PPOX protein expression could be involved in the fluorescence intensity of LPDED in EGC, possibly reflecting histopathological features., (Copyright © 2018 The Authors. Published by Elsevier B.V. All rights reserved.) more...
- Published
- 2018
- Full Text
- View/download PDF
36. Left atrial low-voltage areas predict atrial fibrillation recurrence after catheter ablation in patients with paroxysmal atrial fibrillation.
- Author
-
Masuda M, Fujita M, Iida O, Okamoto S, Ishihara T, Nanto K, Kanda T, Tsujimura T, Matsuda Y, Okuno S, Ohashi T, Tsuji A, and Mano T
- Subjects
- Aged, Atrial Fibrillation diagnosis, Body Surface Potential Mapping methods, Female, Follow-Up Studies, Humans, Male, Middle Aged, Predictive Value of Tests, Prospective Studies, Atrial Fibrillation physiopathology, Atrial Fibrillation surgery, Atrial Function, Left physiology, Catheter Ablation trends
- Abstract
Background: Association between the presence of left atrial low-voltage areas and atrial fibrillation (AF) recurrence after pulmonary vein isolation (PVI) has been shown mainly in persistent AF patients. We sought to compare the AF recurrence rate in paroxysmal AF patients with and without left atrial low-voltage areas., Methods: This prospective observational study included 147 consecutive patients undergoing initial ablation for paroxysmal AF. Voltage mapping was performed after PVI during sinus rhythm, and low-voltage areas were defined as regions where bipolar peak-to-peak voltage was <0.50mV., Results: Left atrial low-voltage areas after PVI were observed in 22 (15%) patients. Patients with low-voltage areas were significantly older (72±6 vs. 66±10, p<0.0001), more likely to be female (68% vs. 32%, p=0.002), and had higher CHA
2 DS2 -VASc score (2.5±1.5 vs. 1.8±1.3, p=0.028). During a mean follow-up of 22 (18, 26) months, AF recurrence was observed in 24 (16%) and 16 (11%) patients after the single and multiple ablation procedures, respectively. AF recurrence rate after multiple ablations was higher in patients with low-voltage areas than without (36% vs. 6%, p<0.001). Low-voltage areas were independently associated with AF recurrence even after adjustment for the other related factors (Hazard ratio, 5.89; 95% confidence interval, 2.16 to 16.0, p=0.001)., Conclusion: The presence of left atrial low-voltage areas after PVI predicts AF recurrence in patients with paroxysmal AF as well as in patients with persistent AF., (Copyright © 2017 Elsevier B.V. All rights reserved.) more...- Published
- 2018
- Full Text
- View/download PDF
37. Comparison of the origin and coupling interval between ectopy with and without atrial fibrillation initiation.
- Author
-
Kanda T, Masuda M, Fujita M, Iida O, Okamoto S, Ishihara T, Nanto K, Sunaga A, Tsujimura T, Matsuda Y, Ohashi T, and Uematsu M
- Subjects
- Aged, Atrial Fibrillation chemically induced, Atrial Fibrillation complications, Atrial Fibrillation surgery, Cardiac Complexes, Premature etiology, Cardiotonic Agents, Catheter Ablation, Female, Humans, Isoproterenol, Male, Middle Aged, Pulmonary Veins surgery, Treatment Outcome, Atrial Fibrillation physiopathology, Cardiac Complexes, Premature physiopathology
- Abstract
Background: Differentiation of atrial fibrillation (AF) trigger ectopy from other ectopy is often difficult. The purpose of this study was to compare the origin and coupling intervals (CI) between AF-trigger and non-AF-trigger ectopy., Methods: This study consisted of 120 patients with AF who underwent an initial ablation. Isoproterenol was infused up to 20μg/min to provoke ectopy and AF. We measured the CI of all ectopy provoked by an isoproterenol infusion. The %CI was calculated as the CI of the ectopy/P-P interval of the preceding 2 beats., Results: A total of 117 patients had at least one ectopy, and AF was induced in 56 (47%) patients. Of the 276 ectopies observed in this study, 211 (76%) originated from pulmonary veins and 77 (28%) were AF-trigger ectopy. AF-trigger ectopy more frequently originated from pulmonary veins (PVs) (74 vs. 3, p<0.001) and had a significantly shorter CI (201±70ms vs. 365±147ms, p<0.001) and lower %CI (29±11% vs. 55±14%, p<0.001) than that of non-AF-trigger ectopy. A receiver operating characteristics analysis revealed that a %CI of 40% was the best cut-off value for differentiating whether it was an AF-trigger or not. The identified trigger group, including patients with provoked AF-trigger ectopy or ectopy with a low %CI (<40%), had a significantly better AF recurrence-free survival rate than the other group (88% vs. 65%, p=0.004)., Conclusions: AF-trigger ectopy predominantly originated from PVs and had a short CI. These findings may be useful for estimating whether ectopies are an AF-trigger or not., (Copyright © 2017 Japanese College of Cardiology. Published by Elsevier Ltd. All rights reserved.) more...
- Published
- 2018
- Full Text
- View/download PDF
38. The identification of conduction gaps after pulmonary vein isolation using a new electroanatomic mapping system.
- Author
-
Masuda M, Fujita M, Iida O, Okamoto S, Ishihara T, Nanto K, Kanda T, Tsujimura T, Matsuda Y, Okuno S, Ohashi T, Tsuji A, and Mano T
- Subjects
- Aged, Atrial Fibrillation physiopathology, Catheter Ablation methods, Feasibility Studies, Female, Follow-Up Studies, Heart Conduction System surgery, Humans, Male, Prospective Studies, Reoperation, Treatment Outcome, Atrial Fibrillation surgery, Body Surface Potential Mapping methods, Heart Atria physiopathology, Heart Conduction System physiopathology, Heart Rate physiology, Pulmonary Veins surgery
- Abstract
Background: The reconnection of left atrial-pulmonary vein (LA-PV) conduction after the initial procedure of pulmonary vein (PV) isolation is not rare, and is one of the main cause of atrial fibrillation (AF) recurrence after PV isolation., Objective: We investigated feasibility of a new ultrahigh-resolution mapping system using a 64-pole small basket catheter for the identification of LA-PV conduction gaps., Methods: This prospective study included 31 consecutive patients (20 with persistent AF) undergoing a second ablation after a PV isolation procedure with LA-PV reconnected conduction at any of the 4 PVs. An LA-PV map was created using the mapping system, and ablation was performed at the estimated gap location., Results: The propagation map identified 54 gaps from 39 ipsilateral PV pairs, requiring manual electrogram reannotation for 23 gaps (43%). Gaps at the anterior and carinal regions of left and right ipsilateral PVs required manual electrogram reannotation more frequently than the other regions. The voltage map could identify the gap only in 19 instances (35%). Electrophysiological properties of the gaps (multiple gaps in the same ipsilateral PVs, conduction time, velocity, width, and length) did not differ between those needing and not needing manual electrogram reannotation. During the gap ablation, either the activation sequence alteration or elimination of PV potentials was observed using a circular catheter placed in the PV, suggesting that all the identified gaps were correct., Conclusion: This new electroanatomic mapping system visualized all the LA-PV gaps in patients undergoing a second AF ablation., (Copyright © 2017 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.) more...
- Published
- 2017
- Full Text
- View/download PDF
39. Sedative and physiological effects of brimonidine tartrate ophthalmic solution in healthy cats.
- Author
-
Ogata N, Kanda T, Kawahata M, Ichikawa T, Matsumoto Y, Morimitsu W, Nishino Y, Itoi T, and Furumoto K
- Subjects
- Adrenergic alpha-2 Receptor Agonists administration & dosage, Animals, Blood Pressure drug effects, Body Temperature drug effects, Brimonidine Tartrate administration & dosage, Cats, Conscious Sedation methods, Cross-Over Studies, Female, Heart Rate drug effects, Male, Ophthalmic Solutions, Respiratory Rate drug effects, Adrenergic alpha-2 Receptor Agonists pharmacology, Brimonidine Tartrate pharmacology, Conscious Sedation veterinary
- Abstract
Objective: To determine the effects of brimonidine tartrate ophthalmic solution on sedation, heart rate (HR), respiratory frequency (f
R ), rectal temperature (RT) and noninvasive mean arterial pressure (MAP) in healthy cats., Study Design: Randomized, blinded crossover study, with 1 week washout between treatments., Animals: Six healthy purpose-bred cats., Methods: Brimonidine tartrate ophthalmic solution 0.1% (one or two drops; 58.6 ± 3.3 μg per drop) or a control solution (artificial tear solution) was administered to six healthy cats. Behavioural observations and measurements of HR, fR , RT and MAP were recorded before and at 30, 60, 90, 120, 180, 240, 300 and 360 minutes after topical administration. Behavioural scores were analysed using Friedman's test for repeated measures to evaluate the time effect in each treatment and treatment effect at each time point. Physiological variables (HR, fR , RT and MAP) were analysed using two-way analysis of variance for repeated measures to evaluate the time and treatment effects. The level of significance was set at p < 0.05., Results: Dose-dependent behavioural and physiological responses were noted. A dose of two drops of brimonidine resulted in sedation in the cats and decreased HR and MAP. Significant sedative effects occurred between 30 and 120 minutes and for physiological responses up to 360 minutes. The most frequent adverse reaction was vomiting, occurring within 40 minutes in all six cats administered two drops and five of the six cats administered one drop of brimonidine., Conclusions and Clinical Relevance: The results demonstrated that ocular administration of brimonidine 0.1% ophthalmic solution induced sedation in cats and some cardiovascular effects usually associated with α2 -adrenoceptor agonists. Further studies should be performed to determine clinical applications for this agent in cats., (Copyright © 2017 Association of Veterinary Anaesthetists and American College of Veterinary Anesthesia and Analgesia. Published by Elsevier Ltd. All rights reserved.) more...- Published
- 2017
- Full Text
- View/download PDF
40. Expression of coproporphyrinogen oxidase is associated with detection of upper gastrointestinal carcinomas by 5-aminolevulinic acid-mediated photodynamic diagnosis.
- Author
-
Ogihara K, Isomoto H, Kurumi H, Kanda T, Hashisako M, Tabata K, Ishii H, Ohnita K, Yamaguchi N, Akazawa Y, Matsushima K, Takeshima F, Kunizaki M, Hidaka S, Nanashima A, Fukuoka J, Nagayasu T, and Nakao K more...
- Subjects
- Adult, Aged, Aged, 80 and over, Female, Humans, Levulinic Acids administration & dosage, Male, Membrane Transport Proteins biosynthesis, Middle Aged, Aminolevulinic Acid, Coproporphyrinogen Oxidase biosynthesis, Photosensitizing Agents administration & dosage, Protoporphyrins pharmacology, Stomach Neoplasms diagnosis, Stomach Neoplasms pathology
- Abstract
Background: 5-Aminolevulinic acid is a precursor of photosensitizing protoporphyrin IX and has been applied for photodynamic diagnosis of brain and bladder tumors with few side effects. Although most upper gastrointestinal tumors can be detected during photodynamic diagnosis, some tumors containing signet-ring cells cannot be visualized. Here, we aimed to assess whether proteins involved in the absorbance, activation, and turnover of protoporphyrin IX altered the fluorescence signal in gastric cancer., Methods: Aminolevulinic acid-mediated photodynamic diagnosis was performed in 23 lesions from 20 patients using an endoscope equipped with a blue laser light that caused red fluorescence emission of photosensitizing protoporphyrin IX. Red fluorescence signal and intensity was assessed during photodynamic diagnosis procedures. Lesions were resected by endoscopic and/or laparoscopic surgery, and specimens were immunostained and assessed for the expression of ATP-binding cassette sub-family G member 2, oligopeptide transporter-1, and coproporphyrinogen oxidase., Results: Photodynamic diagnosis was negative in four cases (17.4%). Three cases of photodynamic diagnosis-negative lesions were signet-ring cell carcinomas, and only one case was differentiated adenocarcinoma (intestinal type). Twenty intestinal type, photodynamic diagnosis-positive lesions showed high expression of coproporphyrinogen oxidase, whereas signet-ring cell carcinomas were all negative. Oligopeptide transporter-1 immunoreactivity was significantly higher in tumors of intestinal type. ATP-binding cassette sub-family G member 2 expression tended to be higher in luminal surface tumors than in intestinal type tumors., Conclusion: Aminolevulinic acid-mediated photodynamic diagnosis provided good detection of upper gastrointestinal tumors of intestinal type but not diffuse type tumors, such as signet-ring cell carcinomas, possibly owing to coproporphyrinogen oxidase expression., (Copyright © 2017 Elsevier B.V. All rights reserved.) more...
- Published
- 2017
- Full Text
- View/download PDF
41. Interferon induces interleukin 8 and bone marrow stromal cell antigen 2 expression, inhibiting the production of hepatitis B virus surface antigen from human hepatocytes.
- Author
-
Haga Y, Kanda T, Nakamoto S, Nakamura M, Sasaki R, Wu S, and Yokosuka O
- Subjects
- Antigens, CD genetics, Antigens, CD immunology, Cell Line, Tumor, Chemokine CXCL10 genetics, Chemokine CXCL10 immunology, GPI-Linked Proteins agonists, GPI-Linked Proteins genetics, GPI-Linked Proteins immunology, Gene Expression Profiling, Gene Expression Regulation, Genes, Reporter, Hep G2 Cells, Hepatitis B Surface Antigens genetics, Hepatitis B Surface Antigens immunology, Hepatitis B virus genetics, Hepatitis B virus pathogenicity, Humans, Interleukin-8 genetics, Interleukin-8 immunology, Luciferases genetics, Luciferases immunology, Poly I-C genetics, Poly I-C immunology, RNA, Messenger genetics, RNA, Messenger immunology, Signal Transduction, Transfection, Chemokine CXCL10 agonists, Hepatitis B Surface Antigens drug effects, Host-Pathogen Interactions, Interferon-alpha pharmacology, Interleukin-8 agonists
- Abstract
Hepatitis B virus (HBV) surface antigen (HBsAg) loss is one of the treatment goals of chronic HBV infection. Bone marrow stromal cell antigen 2 (BST2) is one of the interferon (IFN)-stimulated genes (ISGs) and inhibits the release of various enveloped viruses. Here we examined the effects of antiviral treatment on HBsAg levels and its intracellular mechanism in HBsAg-producing hepatocytes. In PLC/PRF/5 and Huh1, IFNα-2a treatment decreased HBsAg levels in their conditioned media. Upregulation of interleukin 8 (IL8), toll-like receptor 2 (TLR2) and interferon gamma-induced protein 10 (IP10) mRNAs was associated with the reduction of HBsAg in both PLC/PRF/5 and Huh1. The HBsAg level was upregulated by knockdown of IL8, TLR2 or IP10. Exogenous addition of IL8 enhanced BST2 promoter activity and BST2 mRNA expression. Additionally, knockdown of IL8 could lead to the downregulation of BST2 mRNA. Transfection of poly(I-C) enhanced IL8 and BST2 mRNA expression and inhibited HBsAg secretion from PLC/PRF/5 cells. In conclusion, IL8 might play an important role in the enhancement of BST2 and be involved in HBsAg eradication., (Copyright © 2017 Elsevier Inc. All rights reserved.) more...
- Published
- 2017
- Full Text
- View/download PDF
42. Promising techniques to illuminate neuromodulatory control of the cerebral cortex in sleeping and waking states.
- Author
-
Kanda T, Ohyama K, Muramoto H, Kitajima N, and Sekiya H
- Subjects
- Animals, Humans, Cerebral Cortex physiology, Neuroimaging methods, Neurotransmitter Agents physiology, Sleep physiology, Wakefulness physiology
- Abstract
Sleep, a common event in daily life, has clear benefits for brain function, but what goes on in the brain when we sleep remains unclear. Sleep was long regarded as a silent state of the brain because the brain seemingly lacks interaction with the surroundings during sleep. Since the discovery of electrical activities in the brain at rest, electrophysiological methods have revealed novel concepts in sleep research. During sleep, the brain generates oscillatory activities that represent characteristic states of sleep. In addition to electrophysiology, opto/chemogenetics and two-photon Ca
2+ imaging methods have clarified that the sleep/wake states organized by neuronal and glial ensembles in the cerebral cortex are transitioned by neuromodulators. Even with these methods, however, it is extremely difficult to elucidate how and when neuromodulators spread, accumulate, and disappear in the extracellular space of the cortex. Thus, real-time monitoring of neuromodulator dynamics at high spatiotemporal resolution is required for further understanding of sleep. Toward direct detection of neuromodulator behavior during sleep and wakefulness, in this review, we discuss developing imaging techniques based on the activation of G-protein-coupled receptors that allow for visualization of neuromodulator dynamics., (Copyright © 2017 Elsevier Ireland Ltd and Japan Neuroscience Society. All rights reserved.) more...- Published
- 2017
- Full Text
- View/download PDF
43. Free fatty acids or high-concentration glucose enhances hepatitis A virus replication in association with a reduction in glucose-regulated protein 78 expression.
- Author
-
Nwe Win N, Kanda T, Nakamura M, Nakamoto S, Okamoto H, Yokosuka O, and Shirasawa H
- Subjects
- Cell Line, Tumor, Down-Regulation, Endoplasmic Reticulum Chaperone BiP, Heat-Shock Proteins genetics, Hepatitis A complications, Hepatitis A virus physiology, Hepatocytes drug effects, Hepatocytes virology, Humans, MAP Kinase Signaling System drug effects, Metabolic Diseases complications, Thapsigargin pharmacology, Fatty Acids, Nonesterified pharmacology, Glucose pharmacology, Heat-Shock Proteins metabolism, Hepatitis A virology, Hepatitis A virus drug effects, Host-Pathogen Interactions, Virus Replication drug effects
- Abstract
Although the interaction between host and hepatitis A virus (HAV) factors could lead to severe hepatitis A, the exact mechanism of acute liver failure caused by HAV infection is not yet fully understood. The effects of metabolic diseases such as dyslipidemia or diabetes mellitus on HAV replication are still unknown. Here, we examined the effects of free fatty acids or high-concentration glucose on HAV replication and the effects on mitogen-activated protein kinase signaling pathway-related genes in human hepatocytes. We discovered a novel effect of free fatty acids or high-concentration glucose on HAV replication in association with a reduction in the expression of glucose-regulated protein 78 (GRP78). We also observed that thapsigargin induced GRP78 expression and inhibited HAV replication. These findings may provide a new interpretation of the relationship between metabolic diseases and severity of hepatitis A and suggest a new understanding of the mechanism of severe HAV infection., (Copyright © 2016 Elsevier Inc. All rights reserved.) more...
- Published
- 2017
- Full Text
- View/download PDF
44. Gadolinium deposition in the brain.
- Author
-
Kanda T, Nakai Y, Oba H, Toyoda K, Kitajima K, and Furui S
- Subjects
- Brain drug effects, Contrast Media adverse effects, Gadolinium adverse effects, Humans, Retrospective Studies, Brain metabolism, Contrast Media metabolism, Gadolinium metabolism, Image Enhancement, Magnetic Resonance Imaging
- Abstract
Gadolinium is highly toxic. Gadolinium-based contrast agents (GBCAs) consist of gadolinium ions and a chelating agent that binds the gadolinium ion tightly in order not to manifest its toxicity. Knowledge regarding gadolinium deposition in patients with normal renal function has advanced dramatically. Since 2014, increasing attention has been given to residual gadolinium known to accumulate in the tissues of patients with normal renal function. High signal intensity on T1-weighted images (T1WI) in the dentate nucleus, globus pallidus, and pulvinar region of the thalamus correlate roughly with the number of previous GBCA administrations. Pathological analyses have revealed that residual gadolinium is deposited not only in these brain regions, but also in extracranial tissues such as liver, skin and bone. The risks attendant with these deposits are unknown. Common sense dictates that gadolinium deposition be kept as low as possible, and that gadolinium contrast agents be used only when absolutely necessary, with preferential use of macrocyclic chelates, which seem to be deposited at lower concentrations., (Copyright © 2016 Elsevier Inc. All rights reserved.) more...
- Published
- 2016
- Full Text
- View/download PDF
45. The Geriatric Nutritional Risk Index is Independently Associated with Prognosis in Patients with Critical Limb Ischemia Following Endovascular Therapy.
- Author
-
Shiraki T, Iida O, Takahara M, Masuda M, Okamoto S, Ishihara T, Nanto K, Kanda T, Fujita M, and Uematsu M
- Subjects
- Aged, Amputation, Surgical statistics & numerical data, Female, Humans, Ischemia complications, Ischemia mortality, Male, Prognosis, Proportional Hazards Models, Retrospective Studies, Risk Factors, Endovascular Procedures adverse effects, Extremities blood supply, Geriatric Assessment, Ischemia diagnosis, Nutrition Assessment
- Abstract
Objectives: Patients with critical limb ischemia (CLI) have poor overall and limb prognosis. Although nutritional status influences overall prognosis, and the Geriatric Nutritional Risk Index (GNRI) is a widely used, simple and well established nutritional status screening method, the association between the GNRI and the overall and limb prognosis of patients with CLI following endovascular therapy (EVT) has not been explored., Methods: Clinical outcomes were retrospectively evaluated in 473 consecutive patients (74 ± 10 years; 59% male) with CLI who underwent EVT. The GNRI on admission was calculated as follows: [14.89 × albumin (g/dL)] + [41.7 × (body weight/ideal body weight)]. Cox proportional hazard analysis was performed to explore the independent association between the GNRI and mortality and major amputation., Results: Patients (53% ambulatory, 38% wheelchair bound, and 9% bedridden) were divided into two groups based on the median GNRI: the higher group (GNRI ≥ 91.2, n = 237) and the lower group (GNRI < 91.2, n = 236). Median follow up duration after EVT was 11.3 months. Three years after EVT, the survival rate (74% in the higher GNRI, and 48% in the lower GNRI, respectively), and limb salvage rate (92% in the higher GNRI, and 84% in the lower GNRI) were significantly lower in the lower GNRI group. GNRI (hazard ratio [HR], 1.03; 95% confidence interval [CI], 1.01-1.05), along with being wheelchair bound (HR, 1.87; 95% CI 1.17-2.97; vs. ambulatory status), being bedridden (HR, 3.10; 95% CI, 1.63-2.97; vs. ambulatory status), being on hemodialysis (HR, 2.33; 95% CI, 1.49-3.64), and having chronic heart failure (HR, 2.22; 95% CI, 1.44-3.43) were the independent predictors of mortality. The GNRI (HR, 1.04; 95% CI, 1.01-1.07), being bedridden (HR, 4.15; 95% CI, 1.67-10.3; vs. ambulatory status), isolated below knee disease (HR, 2.49; 95% CI, 1.30-4.77), and hemodialysis (HR, 2.44; 95% CI, 1.23-4.85) were independently associated with major amputation., Conclusions: The GNRI on admission was independently associated with mortality and major amputation after EVT in patients with CLI., (Copyright © 2016 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.) more...
- Published
- 2016
- Full Text
- View/download PDF
46. Endovascular Treatment for Proximal Anastomotic Pseudoaneurysm after Total Arch Replacement in Behcet's Disease.
- Author
-
Sakata T, Ueda H, Watanabe M, Kohno H, Tamura Y, Abe S, Inage Y, Ikeuchi H, Kanda T, Fujii M, and Matsumiya G
- Subjects
- Adolescent, Anastomosis, Surgical, Aneurysm, False diagnostic imaging, Aneurysm, False etiology, Aortic Aneurysm, Thoracic diagnostic imaging, Aortic Aneurysm, Thoracic etiology, Aortography methods, Behcet Syndrome diagnosis, Blood Vessel Prosthesis, Blood Vessel Prosthesis Implantation instrumentation, Female, Humans, Prosthesis Design, Reoperation, Stents, Tomography, X-Ray Computed, Treatment Outcome, Aneurysm, False surgery, Aortic Aneurysm, Thoracic surgery, Behcet Syndrome complications, Blood Vessel Prosthesis Implantation adverse effects, Endovascular Procedures instrumentation
- Abstract
A 17-year-old patient underwent total arch replacement for aortic arch aneurysm due to vascular Behcet's disease (BD). Follow-up computed tomography, performed 6 months after the operation, demonstrated pseudoaneurysm formation at the proximal anastomotic site. We performed endovascular treatment and used a short stent graft that was originally designed for abdominal aortic aneurysm. To avoid the occlusion of the coronary or brachiocephalic artery (BCA) due to stent graft migration, we used right ventricular rapid pacing and BCA ballooning. Thus, we believe that endovascular treatment can be used for anastomotic complications in the ascending aorta after open surgery for connective tissue disorders including BD., (Copyright © 2016 Elsevier Inc. All rights reserved.) more...
- Published
- 2016
- Full Text
- View/download PDF
47. Photodynamic therapy using Photofrin and excimer dye laser treatment for superficial oral squamous cell carcinomas with long-term follow up.
- Author
-
Toratani S, Tani R, Kanda T, Koizumi K, Yoshioka Y, and Okamoto T
- Subjects
- Adult, Aged, Aged, 80 and over, Carcinoma, Squamous Cell pathology, Carcinoma, Squamous Cell radiotherapy, Color, Female, Follow-Up Studies, Humans, Male, Middle Aged, Mouth Neoplasms pathology, Mouth Neoplasms radiotherapy, Photosensitizing Agents therapeutic use, Retrospective Studies, Time, Treatment Outcome, Carcinoma, Squamous Cell therapy, Dihematoporphyrin Ether therapeutic use, Lasers, Dye therapeutic use, Mouth Neoplasms therapy
- Abstract
Objectives: Photodynamic therapy (PDT) is a very effective treatment for superficial malignancies that does not result in loss of normal tissue. Here, we report successful PDT treatment of superficial oral cancers and its clinical outcome with long-term follow up., Materials and Methods: Thirty-four superficial oral squamous cell carcinomas were treated with PDT, and the effects were evaluated. Each patient received Photofrin (2mg/kg) intravenously 48h prior to light irradiation. Photoradiation was performed at doses of 100-150J/cm(2) using a 630-nm wavelength excimer dye laser., Results: Six months after PDT, 30 patients (88.2%) showed complete responses while 9 patients (26.5%) had local relapses during long-term follow-up. The 5-year overall survival, disease-specific survival, and disease-free survival rates were 76.5%, 84.6%, and 63.3%, respectively. Lesions with red patches had a significantly higher recurrence rate than lesions with white patches. Accurate evaluation of the extent of lesions and appropriate photoradiation were important in improving outcomes. Adverse events observed included sunburn and sequestrum formation of alveolar bone. No abnormal laboratory values or systemic complications were observed., Conclusion: PDT using Photofrin as the photosensitizer is an effective treatment modality for superficial oral carcinomas, with excellent healing and minimal side effects., (Copyright © 2015 Elsevier B.V. All rights reserved.) more...
- Published
- 2016
- Full Text
- View/download PDF
48. Influence of underlying substrate on atrial tachyarrhythmias after pulmonary vein isolation.
- Author
-
Masuda M, Fujita M, Iida O, Okamoto S, Ishihara T, Nanto K, Kanda T, Shiraki T, Sunaga A, Matsuda Y, and Uematsu M
- Subjects
- Aged, Atrial Fibrillation surgery, Female, Follow-Up Studies, Heart Conduction System physiopathology, Humans, Male, Middle Aged, Postoperative Period, Prospective Studies, Time Factors, Treatment Outcome, Atrial Fibrillation physiopathology, Body Surface Potential Mapping, Catheter Ablation methods, Heart Conduction System surgery, Pulmonary Veins surgery
- Abstract
Background: Recurrent atrial tachyarrhythmias occur as a result of residual atrial arrhythmogenic substrates after atrial fibrillation (AF) ablation. In patients with AF, electrograms with reduced amplitudes indicate diseased myocardium., Objective: The purpose of this study was to investigate the association between the distribution of low-voltage areas and the type of induced atrial tachyarrhythmias., Methods: Our prospective observational study enrolled 152 consecutive AF patients scheduled for an initial ablation (46% persistent AF). After pulmonary vein isolation, voltage mapping was performed during sinus rhythm, and regions with reduced electrogram amplitudes (<0.5 mV) were defined as low-voltage areas. Burst pacing was performed to investigate the inducibility of atrial tachyarrhythmias., Results: Low-voltage areas were more frequently observed in patients with persistent AF than paroxysmal AF (50% vs. 34%, P = .048). A higher proportion of patients with low-voltage areas presented with inducibility of atrial tachyarrhythmias than those without, as follows: AF 70% vs. 16% (P = .0001); perimitral macroreentrant atrial tachycardia (AT) 18% vs. 0% (P = .0001); and roof-dependent macroreentrant AT 13% vs. 0% (P = .01). Investigation into the regional distribution of low-voltage areas revealed that patients with perimitral macroreentrant AT more frequently coincided with low-voltage areas than those without in the septal (100% vs. 18%, P <.0001) and anterior regions (55% vs. 11%, P = .001), and those with roof-dependent AT in the roof (75% vs. 15%, P <.0001) and posterior regions (75% vs. 15%, P = .0001)., Conclusion: Low-voltage areas are associated with high inducibility of atrial tachyarrhythmias after pulmonary vein isolation. In addition, the distribution of low-voltage areas is specific for each type of macroreentrant AT., (Copyright © 2016 Heart Rhythm Society. Published by Elsevier Inc. All rights reserved.) more...
- Published
- 2016
- Full Text
- View/download PDF
49. Effects of medetomidine and xylazine on intraocular pressure and pupil size in healthy Beagle dogs.
- Author
-
Kanda T, Iguchi A, Yoshioka C, Nomura H, Higashi K, Kaya M, Yamamoto R, Kuramoto T, and Furukawa T
- Subjects
- Adrenergic alpha-2 Receptor Agonists administration & dosage, Animals, Cross-Over Studies, Dogs, Dose-Response Relationship, Drug, Injections, Intramuscular veterinary, Male, Medetomidine administration & dosage, Xylazine administration & dosage, Adrenergic alpha-2 Receptor Agonists pharmacology, Intraocular Pressure drug effects, Medetomidine pharmacology, Reflex, Pupillary drug effects, Xylazine pharmacology
- Abstract
Objective: To determine the effects of intramuscular (IM) administration of medetomidine and xylazine on intraocular pressure (IOP) and pupil size in normal dogs., Study Design: Prospective, randomized, experimental, crossover trial., Animals: Five healthy, purpose-bred Beagle dogs., Methods: Each dog was administered 11 IM injections of, respectively: physiological saline; medetomidine at doses of 5, 10, 20, 40 and 80 μg kg(-1), and xylazine at doses of 0.5, 1.0, 2.0, 4.0 and 8.0 mg kg(-1). Injections were administered at least 1 week apart. IOP and pupil size were measured at baseline (before treatment) and at 0.25, 0.50, 0.75, 1, 2, 3, 4, 5, 6, 7, 8 and 24 hours post-injection., Results: A significant decrease in IOP was observed at 6 hours after 80 μg kg(-1) medetomidine compared with values at 0.25 and 0.50 hours, although there were no significant changes in IOP from baseline. In dogs treated with 8.0 mg kg(-1) xylazine, significant reductions in IOP were observed at 4 and 5 hours compared with that at 0.25 hours after administration. In dogs treated with 5, 10, 20 and 40 μg kg(-1) medetomidine and 0.5, 1.0 and 2.0 mg kg(-1) xylazine, there were no significant changes in IOP. Pupil size did not change significantly after any of the medetomidine or xylazine treatments compared with the baseline value., Conclusions and Clinical Relevance: Low or moderate doses of medetomidine or xylazine did not induce significant changes in IOP or pupil size. In contrast, high doses of medetomidine or xylazine induced significant changes up to 8 hours after treatment, but values remained within the normal canine physiological range. The results of this study suggest a lack of significant change in IOP and pupil size in healthy dogs administered low or moderate doses of xylazine or medetomidine., (© 2015 Association of Veterinary Anaesthetists and the American College of Veterinary Anesthesia and Analgesia.) more...
- Published
- 2015
- Full Text
- View/download PDF
50. Low left atrial appendage flow velocity predicts recurrence of atrial fibrillation after catheter ablation of persistent atrial fibrillation.
- Author
-
Kanda T, Masuda M, Sunaga A, Fujita M, Iida O, Okamoto S, Ishihara T, Watanabe T, Takahara M, Sakata Y, and Uematsu M
- Subjects
- Aged, Atrial Fibrillation mortality, Atrial Fibrillation surgery, Blood Flow Velocity, Catheter Ablation mortality, Echocardiography, Transesophageal, Female, Humans, Kaplan-Meier Estimate, Male, Middle Aged, Proportional Hazards Models, Pulmonary Veins surgery, Recurrence, Survival Rate, Treatment Outcome, Atrial Appendage physiopathology, Atrial Fibrillation physiopathology, Atrial Function, Left physiology, Catheter Ablation adverse effects
- Abstract
Background: Recurrence after catheter ablation of persistent atrial fibrillation (AF) remains an unsolved issue. This study aimed to explore the association between the left atrial appendage peak flow velocity (LAAV) and AF recurrence after ablation in persistent AF patients., Methods: Fifty-three consecutive patients who underwent an initial catheter ablation of persistent AF were enrolled [age, 65±10 years; male, 42 (79%)]. The LAAV was obtained by transesophageal echocardiography before ablation. All the patients underwent pulmonary vein isolation and were followed up for 12 months. The LAAV and other clinical factors (AF duration, CHA2DS2VASc score, left atrial diameter, left atrial volume, and left ventricular ejection fraction) were tested using a Cox proportional hazards regression analysis as predictors of AF recurrence during the 1-year follow-up., Results: AF recurrence occurred in 16 (30%) patients. The patients with AF recurrences had lower LAAVs (23.3±7.2cm/s vs. 33.3±15.1cm/s, p=0.002) than those without AF recurrence. In the multivariable analysis, a low LAAV independently predicted AF recurrence (hazard ratio, 3.04; 95% confidence interval, 1.05-8.79; p=0.040). A Kaplan-Meier analysis also demonstrated a lower survival rate free from AF recurrence in the low LAAV group than in the high LAAV group (p=0.030)., Conclusion: A low LAAV was associated with AF recurrence after the initial catheter ablation of persistent AF., (Copyright © 2015. Published by Elsevier Ltd.) more...
- Published
- 2015
- Full Text
- View/download PDF
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.