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To accurately predict heat transfer coefficients for hydrocarbon refrigerants condensation flow in heat exchangers is imperative for designing efficient systems. While various studies have proposed numerous correlations for predicting heat transfer coefficients, some of these correlations are based on fitted experimental data and are limited to predicting specific conditions. The prediction accuracy and capability for different working fluids remain to be evaluated. Currently, most researches mainly focus on pure working fluids, with relatively little attention given to mixed working fluids. In this study, six commonly used heat transfer correlations are evaluated and a new heat transfer correlation based on flow patterns is proposed. The research results indicate that the existing correlations have poor universality for predicting hydrocarbon refrigerants, and the prediction accuracy needs to be improved. The correlation developed in this study demonstrates good prediction performance for condensation heat transfer coefficients of 413 sets of hydrocarbon refrigerants, including methane, propane, ethane/propane, and methane/propane. The overall mean relative deviation (MRD) is −1.77%, and mean absolute relative deviation (MARD) is 8.12%. The correlation developed in this study can provide theoretical guidance for predicting condensation heat transfer coefficient for pure and mixed hydrocarbon refrigerants, aiding in the design of efficient heat exchangers.

Summary: The fluorescent non-canonical amino acid tagging (FUNCAT) technique has been used to visualize newly synthesized proteins in cell lines and tissues. Here, we present a protocol for measuring protein synthesis in specific cell types in the mouse brain using in vivo FUNCAT. We describe steps for metabolically labeling newly synthesized proteins with azidohomoalanine, which introduces an azide group into the polypeptide. We then detail procedures for binding a fluorophore-conjugated alkyne to the azide group to allow its visualization.For complete details on the use and execution of this protocol, please refer to tom Dieck et al. (2012)1 and Hooshmandi et al. (2023).2 : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Gas submerged jet is an outstanding thermohydraulic phenomenon in pool scrubbing of fission products during a severe nuclear accident. Experiments were performed on the hydraulic characteristics in the ranges of air mass flux 0.1–1400 kg/m2s and nozzle diameter 10–80 mm. The results showed that the dependence of inlet pressure on the mass flux follows a power law in subsonic jets and a linear law in sonic jets. The effect of nozzle submerged depth was negligible. The isolated bubbling regime, continuous bubbling regime, transition regime, and jetting regime were observed in turn, as the mass flux increased. In the bubbling regime and jetting regime, the air volume fraction distribution was approximately symmetric in space. Themelis model could capture the jet trajectory well. In the transition regime, the air volume fraction distribution loses symmetry due to the bifurcated secondary plume. The Li correlation and Themelis model showed sufficient accuracy for the prediction of jet penetration length.

Twisted tri-lobed tubes have garnered attention due to their exceptional heat transfer efficiency and straightforward fabrication. Existing literature lacks comprehensive assessments of the overall heat transfer performance of twisted tri-lobed tubes from the perspective of energy loss and irreversibility. This research aims to investigate entropy generation during turbulent water flow within twisted tri-lobed tubes, examining the influence of geometric parameters on local and average entropy production. Findings indicate that larger small circle radius (r) and straight lengths (l), coupled with smaller transition circle radius (R) and twist pitch lengths (p), result in diminished local and average heat transfer entropy production while enhancing local and average frictional entropy production, with heat transfer entropy generation dominating the overall entropy production. Additionally, with increasing Reynolds numbers, all twisted tubes demonstrate an increasing trend in average frictional entropy production, except for some cases (Case 1-Case 4) that exhibit an initial rise followed by a decline in average heat transfer entropy generation. Among the examined Reynolds range, Case 4 displays lower overall irreversibility compared to a plain tube. Following the second law of thermodynamics, Case 4 is preferred. The findings and methodology contribute to enhancing the thermodynamic evaluation of convective heat transfer in twisted tri-lobed tubes.

Osteosarcoma (OS) is a malignant bone tumor that mainly occurs in adolescents. It is accompanied by a high rate of lung metastasis, and high mortality. Recent studies have suggested the important roles of tripartite motif-containing (TRIM) family proteins in regulating various substrates and signaling pathways in different tumors. However, the detailed functional role of TRIM family proteins in the progression of OS is still unknown and requires further investigations. In this study, we found that tripartite motif-containing 22 (TRIM22) was downregulated in OS tissues and was hence associated with better prognosis. In vitro and in vivo functional analysis demonstrated that TRIM22 inhibits proliferation and metastasis of OS cells. Nuclear factor erythroid 2-related factor 2 (NRF2), a redox regulator, was identified as a novel target for TRIM22. TRIM22 interacts with and accelerates the degradation of NRF2 by inducing its ubiquitination dependent on its E3 ligase activity but independent of Kelch-like ECH-associated protein 1 (KEAP1). Further, a series of gain- and loss-of-function experiments showed that knockdown or overexpression of NRF2 reversed the functions of knockdown or overexpression of TRIM22 in OS. Mechanistically, TRIM22 inhibited OS progression through NRF2-mediated intracellular reactive oxygen species (ROS) imbalance. ROS production was significantly promoted and mitochondrial potential was remarkably inhibited when overexpressing TRIM22, thus activating AMPK/mTOR signaling. Moreover, TRIM22 was also found to inhibit Warburg effect in OS cells. Autophagy activation was found in OS cells which were overexpressed TRIM22, thus leading to autophagic cell death. Treatment with N-Acetylcysteine (NAC), a ROS scavenger or the autophagy inhibitor 3-Methyladenine (3-MA) abolished the decreased malignant phenotypes in TRIM22 overexpressing OS cells. In conclusion, our study indicated that TRIM22 inhibits OS progression by promoting proteasomal degradation of NRF2 independent of KEAP1, thereby activating ROS/AMPK/mTOR/Autophagy signaling that leads to autophagic cell death in OS. Therefore, our findings indicated that targeting TRIM22/NRF2 could be a promising therapeutic target for treating OS.

Increasing evidence indicates that lymphocyte cytosolic protein 1 (LCP1) overexpression contributes to tumor progression; however, its role in osteosarcoma (OS) remains unclear. We aimed to investigate the potential effect of LCP1 in OS and the underlying mechanisms. We first demonstrated that LCP1 is upregulated in OS cell lines and tissues. Then, we found that aberrant expression of LCP1 could induce the proliferation and metastasis of OS cells in vitro and in vivo by destabilizing neuregulin receptor degradation protein-1 (Nrdp1) and subsequently activating the JAK2/STAT3 signaling pathway. When coculturing OS cells with bone marrow-derived mesenchymal stem cells (BMSCs) in vitro, we validated that oncogenic LCP1 in OS was transferred from BMSCs via exosomes. Moreover, microRNA (miR)-135a-5p, a tumor suppressor, was found to interact upstream of LCP1 to counteract the pro-tumorigenesis effects of LCP1 in OS. In conclusion, BMSC-derived exosomal LCP1 promotes OS proliferation and metastasis via the JAK2/STAT3 pathway. Targeting the miR-135a-5p/LCP1 axis may have potential in treating OS.

Pathologically, blood-spinal-cord-barrier (BSCB) disruption after spinal cord injury (SCI) leads to infiltration of numerous peripheral macrophages into injured areas and accumulation around newborn vessels. Among the leaked macrophages, M1-polarized macrophages are dominant and play a crucial role throughout the whole SCI process. The aim of our study was to investigate the effects of M1-polarized bone marrow-derived macrophages (M1-BMDMs) on vascular endothelial cells and their underlying mechanism. Microvascular endothelial cell line bEnd.3 cells were treated with conditioned medium or exosomes derived from M1-BMDMs, followed by evaluations of endothelial-to-mesenchymal transition (EndoMT) and mitochondrial function. After administration, we found conditioned medium or exosomes from M1-BMDMs significantly promoted EndoMT of vascular endothelial cells in vitro and in vivo, which aggravated BSCB disruption after SCI. In addition, significant dysfunction of mitochondria and accumulation of reactive oxygen species (ROS) were also detected. Furthermore, bioinformatics analysis demonstrated that miR-155 is upregulated in both M1-polarized macrophages and microglia. Experimentally, exosomal transfer of miR-155 participated in M1-BMDMs-induced EndoMT and mitochondrial ROS generation in bEnd.3 cells, and subsequently activated the NF-κB signaling pathway by targeting downstream suppressor of cytokine signaling 6 (SOCS6), and suppressing SOCS6-mediated p65 ubiquitination and degradation. Finally, a series of rescue assay further verified that exosomal miR155/SOCS6/p65 axis regulated the EndoMT process and mitochondrial function in vascular endothelial cells. In summary, our work revealed a potential mechanism describing the communications between macrophages and vascular endothelial cells after SCI which could benefit for future research and aid in the development of potential therapies for SCI.