49 results on '"Xu, Jingwen"'
Search Results
2. List of contributors
- Author
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Cai, Xinyi, primary, Duan, Lian, additional, He, Yanmei, additional, Jiang, Ruming, additional, Li, Qian, additional, Qiao, Juan, additional, Su, Shi-Jian, additional, Tang, Ben Zhong, additional, Tsuboi, Taiju, additional, Wang, Dan, additional, Wang, Liangying, additional, Xu, Jingwen, additional, Xue, Jie, additional, Yang, Chuluo, additional, Zeng, Jiajie, additional, Zeng, Weixuan, additional, Zhang, Dongdong, additional, Zhang, Qisheng, additional, Zhang, Xiaohong, additional, Zhao, Zujin, additional, and Zheng, Caijun, additional
- Published
- 2022
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3. Phenotypic Diversity of Colored Phytochemicals in Sorghum Accessions With Various Pericarp Pigments
- Author
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Davis, Haley, primary, Su, Xiaoyu, additional, Shen, Yanting, additional, Xu, Jingwen, additional, Wang, Donghai, additional, Scott Smith, J., additional, Aramouni, Fadi, additional, and Wang, Weiqun, additional
- Published
- 2019
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4. Contributors
- Author
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Achmadi, Suminar Setiati, primary, Aguilar, Cristóbal N., additional, Aguirre, A., additional, Amenta, Margherita, additional, Andabaka, Željko, additional, Aramouni, Fadi, additional, Ašperger, Danijela, additional, Ballistreri, Gabriele, additional, Barros, Ana I.R.N.A., additional, Borneo, R., additional, Bujor, Oana-Crina, additional, Caires, Anderson Rodrigues Lima, additional, Campillo, Natalia, additional, Carle, Reinhold, additional, Chedea, Veronica Sanda, additional, Cheetangdee, Nopparat, additional, Contreras-Esquivel, Juan C., additional, Davis, Haley, additional, de Oliveira, Ivan Pires, additional, Fabroni, Simona, additional, Freitas de Lima, Fernando, additional, Goula, Athanasia, additional, Gouvinhas, Irene, additional, Hofmann, Tamás, additional, Hoskin, David W., additional, Huzanić, Nera, additional, Ioannou, Aristodimos, additional, Kammerer, Dietmar Rolf, additional, Kammerer, Judith, additional, Kontić, Jasminka Karoglan, additional, Ledesma-Escobar, Carlos A., additional, Lescano, Caroline Honaiser, additional, Loung, Chao-Yu, additional, Luque de Castro, María D., additional, Maletić, Edi, additional, Mourtzinos, Ioannis, additional, Mu, Tai-Hua, additional, Muñiz-Márquez, Diana B., additional, Oreopoulou, Antigoni, additional, Oreopoulou, Vassiliki, additional, Passon, Maike, additional, Pop, Raluca Maria, additional, Popa, Valentin I., additional, Preiner, Darko, additional, Priego-Capote, Feliciano, additional, Pyrzynska, Krystyna, additional, Queiroz, Marcelo, additional, Rapisarda, Paolo, additional, Rasmussen, Andrea N., additional, Rodrigues, Miguel, additional, Rodriguez-Herrera, Raúl, additional, Romeo, Flora Valeria, additional, Ruberto, Giuseppe, additional, Scott Smith, J., additional, Sentkowska, Aleksandra, additional, Shen, Yanting, additional, Siracusa, Laura, additional, Stupić, Domagoj, additional, Su, Xiaoyu, additional, Sun, Hong-Nan, additional, Tanase, Corneliu, additional, Timpanaro, Nicolina, additional, Tomaz, Ivana, additional, Tsimogiannis, Dimitrios, additional, Varotsis, Constantinos, additional, Viñas, Pilar, additional, Wang, Donghai, additional, Wang, Weiqun, additional, Weber, Fabian, additional, Wong-Paz, Jorge E., additional, and Xu, Jingwen, additional
- Published
- 2019
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5. Aggregation-induced delayed fluorescence
- Author
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Zeng, Jiajie, Xu, Jingwen, Jiang, Ruming, Zhao, Zujin, Tang, Benzhong, Zeng, Jiajie, Xu, Jingwen, Jiang, Ruming, Zhao, Zujin, and Tang, Benzhong
- Abstract
High exciton utilization is essential to improve the efficiency of organic light-emitting diodes (OLEDs). Thermally activated delayed fluorescence (TADF) emitters can provide ~100% electrogenerated exciton utilization via rapid reverse intersystem crossing process and have gained wide attention as they are low cost compared to noble metal-containing phosphorescent emitters. However, traditional TADF emitters usually suffer from concentration quenching and exciton annihilation, resulting in sophisticated fabrication process and severe efficiency roll-off at high luminance. To solve this problem, TADF emitters are endowed with aggregation-induced emission, giving rise to novel aggregation-induced delayed fluorescence (AIDF) luminogens, which are proved to be promising candidates for both efficient nondoped and doped OLEDs, where they can act as emitter or host. In this chapter, the molecular design, photophysical characteristics, and device performances of newly developed AIDF luminogens are systematically demonstrated, which could be a reference for developing stable and efficient OLEDs for real-world applications.
- Published
- 2022
6. Cytotoxic and anti-inflammatory polyacetylenes from Tridax procumbens L.
- Author
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Tan Z, Chen C, Chen L, Zeng J, Zhang W, Xu J, He X, and Wang Y
- Subjects
- Humans, Mice, Animals, Apoptosis drug effects, Drug Screening Assays, Antitumor, RAW 264.7 Cells, Molecular Structure, Dose-Response Relationship, Drug, Lipopolysaccharides pharmacology, Lipopolysaccharides antagonists & inhibitors, Asteraceae chemistry, K562 Cells, Structure-Activity Relationship, Cell Proliferation drug effects, Nitric Oxide antagonists & inhibitors, Nitric Oxide biosynthesis, NF-kappa B metabolism, NF-kappa B antagonists & inhibitors, Cell Line, Tumor, Polyynes pharmacology, Polyynes chemistry, Polyynes isolation & purification, Antineoplastic Agents, Phytogenic pharmacology, Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic isolation & purification, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents isolation & purification
- Abstract
Herein, 17 previously undescribed polyacetylenes and 9 known ones were isolated from Tridax procumbens L. Their structures were identified using spectroscopic techniques (NMR, UV, IR, MS and optical rotation), the modified Mosher method, electronic circular dichroism (ECD) data and ECD calculation. The cytotoxicity of polyacetylenes on six human tumour cell lines (K562, K562/ADR, AGS, MGC-803, SPC-A-1 and MDA-MB-231) was evaluated. (3S,10R)-tridaxin B (2a), (3S,10S)-tridaxin B (2b) and tridaxin F (8) demonstrated substantial cytotoxic effects against the K562 cell line, with half-maximal inhibitory concentration (IC
50 ) values of 2.62, 14.43 and 17.91 μM, respectively. Cell and nucleus morphology assessments and Western blot analysis confirmed that the cytotoxicity of the three polyacetylenes on K562 cells was mediated through a dose-dependent apoptosis pathway. Furthermore, (3S,10R)-tridaxin A (1a) and tridaxin G (9) exhibited considerable inhibitory effects on lipopolysaccharide-stimulated nitric oxide production in RAW 264.7 macrophages, with IC50 values of 15.92 and 20.35 μM, respectively. Further investigations revealed that 9 exerted anti-inflammatory activities by impeding the nuclear translocation of NF-κB and down-regulating the expression of pro-inflammatory factors, including those of iNOS, COX-2, IL-1β and IL-6, in a concentration-dependent manner. The study provides evidence that polyacetylenes from T. procumbens may serve as a potential source of anti-tumour or anti-inflammatory agents for treating related diseases., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
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7. PLA tissue-engineered scaffolds loaded with sustained-release active substance chitosan nanoparticles: Modeling BSA-bFGF as the active substance.
- Author
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Shen W, Mao Y, Ge X, Xu J, Hu J, Ao F, Wu S, and Yan P
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- Animals, Porosity, Biocompatible Materials chemistry, Cattle, Chitosan chemistry, Polyesters chemistry, Tissue Engineering methods, Tissue Scaffolds chemistry, Nanoparticles chemistry, Delayed-Action Preparations, Serum Albumin, Bovine chemistry
- Abstract
The utilization of basic fibroblast growth factor (bFGF) in the development of tissue-engineered scaffolds is both challenging and imperative. In our pursuit of creating a scaffold that aligns with the natural healing process, we initially fabricated chitosan-bFGF nanoparticles (CS-bFGF NPs) through electrostatic spraying. Subsequently, polylactic acid (PLA) fiber was prepared using electrospinning technique, and the CS-bFGF NPs were uniformly embedded within the pores of porous PLA fibers. Scanning electron micrographs illustrate the smooth surface of the nanoparticles, showing a porous structure intricately attached to PLA fibers. Fourier-transform infrared spectroscopy (FTIR), energy-dispersive X-ray spectroscopy (EDS), and X-ray diffraction (XRD) analyses provided conclusive evidence that the CS-bFGF NPs were uniformly distributed throughout the porous PLA fibers, forming a robust physical bond through electrostatic adsorption. The resultant scaffolds exhibited commendable mechanical properties and hydrophilicity, facilitating a sustained-release for 72 h. Furthermore, the biocompatibility and degradation performance of the scaffolds were substantiated by monitoring conductivity and pH changes in pure water over different time intervals, complemented by scanning electron microscopy (SEM) observations. Cell experiments confirmed the cytocompatibility of the scaffolds. In animal studies, the group treated with 16 % NPs/Scaffold demonstrated the highest epidermal reconstruction rate. In summary, our developed materials present a promising candidate for serving as a tissue engineering scaffold, showcasing exceptional biocompatibility, sustained-release characteristics, and substantial potential for promoting epidermal regeneration., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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8. RpoS sigma factor mediates adaptation and virulence in Vibrio mimicus.
- Author
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Jiang Z, Chen A, Chen Z, Xu J, Gao X, Jiang Q, and Zhang X
- Subjects
- Virulence genetics, Adaptation, Physiological, Stress, Physiological, Sigma Factor genetics, Sigma Factor metabolism, Bacterial Proteins genetics, Bacterial Proteins metabolism, Gene Expression Regulation, Bacterial, Biofilms growth & development, Vibrio mimicus pathogenicity, Vibrio mimicus genetics, Vibrio mimicus metabolism
- Abstract
The alternative sigma factor RpoS functions as a regulator of stress and virulence response in numerous bacterial species. Vibrio mimicus is a critical opportunistic pathogen causing huge losses to aquaculture. However, the exact role of RpoS in V. mimicus remains unclear. In this study, rpoS deletion mutant of V. mimicus was constructed through allelic exchange and the phenotypic and transcriptional changes were investigated to determine the function of RpoS. The abilities of growth, motility, biofilm production, hemolytic activity and pathogenicity were significantly impaired in ΔrpoS strain. Stationary-phase cells of ΔrpoS strain showed lower tolerance to H
2 O2 , heat, ethanol, and starvation stress than the wild-type strain. Transcriptome analyses revealed the involvement of rpoS in various cellular processes, notably bacterial-type flagellum synthesis and assembly, membrane synthesis and assembly and response to various stimuli. Phenotypic and RNA-seq analysis revealed that RpoS is required for biofilm formation, stress resistance, and pathogenicity in V. mimicus. Furthermore, β-galactosidase activity showed that rpoS is essential for optimal transcription of the flgK, fliA, cheA, mcpH mRNA. These results offer significant insight into the function and regulatory network of rpoS/RpoS, thereby improving our understanding and facilitating selection of molecular targets for future prevention strategies against V. mimicus., Competing Interests: Declaration of competing interest The authors declare no conflict of interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)- Published
- 2024
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9. Litter quality and climate regulate the effect of invertebrates on litter decomposition in terrestrial and aquatic ecosystems: A global meta-analysis.
- Author
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Zhan Z, Wang S, Huang J, Cai J, Xu J, Zhou X, Wang B, and Chen D
- Subjects
- Animals, Climate, Biodegradation, Environmental, Aquatic Organisms, Invertebrates physiology, Ecosystem
- Abstract
Although the exclusion effects of invertebrate decomposers on litter decomposition have been extensively studied in different experimental contexts, a thorough comparison of the exclusion effects of invertebrate decomposers with different body sizes on litter decomposition and its possible regulatory factors in terrestrial and aquatic ecosystems is still lacking. Here, through a meta-analysis of 1207 pairs of observations from 110 studies in terrestrial ecosystems and 473 pairs of observations from 60 studies in aquatic ecosystems, we found that invertebrate exclusion reduced litter decomposition rates by 36 % globally, 30 % in terrestrial ecosystems, and 44 % in aquatic ecosystems. At the global scale, the exclusion effects of macroinvertebrates and mesoinvertebrates on litter decomposition rates (reduced by 38 % and 36 %, respectively) were greater than those of the combination of macroinvertebrates and mesoinvertebrates (reduced by 30 %). In terrestrial and aquatic ecosystems, the effects of invertebrate exclusion on litter decomposition rates were mainly regulated by climate and initial litter quality, but the effects of invertebrate exclusion with different body sizes were regulated differently by climate, initial litter quality, and abiotic environmental variables. These findings will help us better understand the role of invertebrate decomposers in litter decomposition, especially for invertebrate decomposers with different body sizes, and underscore the need to incorporate invertebrate decomposers with different body sizes into dynamic models of litter decomposition to examine the potential effects and regulatory mechanisms of land-water-atmosphere carbon fluxes., Competing Interests: Declaration of competing interest The authors declare that they have no Declaration of competing interest., (Copyright © 2024 Elsevier B.V. All rights reserved.)
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- 2024
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10. Six new phenylpropanoids from Kaempferia galanga L. and their anti-inflammatory activity.
- Author
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Zhu Y, Chen L, Zeng J, Xu J, Hu H, He X, and Wang Y
- Subjects
- RAW 264.7 Cells, Mice, Animals, Molecular Structure, Nitric Oxide Synthase Type II metabolism, China, Cyclooxygenase 2 metabolism, Zingiberaceae chemistry, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents isolation & purification, NF-kappa B metabolism, NF-kappa B antagonists & inhibitors, Rhizome chemistry, Phytochemicals pharmacology, Phytochemicals isolation & purification, Nitric Oxide metabolism
- Abstract
Kaempferia galanga L. is an aromatic medicinal plant belonging to the Zingiberaceae family. Its rhizome has been widely used as traditional Chinese medicine and a flavor spice for a long time. In this study, six previously undescribed phenylpropanoids, including four [2+2]-cycloaddition-derived cyclobutane natural products (1-4), and two phenylpropanoids (5-6) were isolated from the rhizomes of K. galanga L. Their structures were elucidated by spectroscopic methods, single-crystal X-ray diffraction, NMR calculation, and ECD spectra calculation. These cyclobutane derivatives were isolated from K. galanga for the first time. Furthermore, compounds 1-6 were evaluated for the potential inhibitory activities on NO production and NF-κB nuclear translocation in LPS-triggered RAW 264.7 macrophages. The results showed that the isolated compounds have a moderate anti-inflammatory activity measured on their potency to inhibit NO production and the expression of iNOS and COX-2. Additionally, compound 2 effectively suppressed NF-κB nuclear translocation at a concentration of 40 μM., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)
- Published
- 2024
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11. Antiproliferative piperidine alkaloids from the leaves of Alocasia macrorrhiza.
- Author
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Deng W, Shen L, Zeng J, Gao J, Luo J, Xu J, Wang Y, and He X
- Subjects
- Humans, Molecular Structure, Structure-Activity Relationship, Dose-Response Relationship, Drug, K562 Cells, Crystallography, X-Ray, Plant Leaves chemistry, Alkaloids pharmacology, Alkaloids chemistry, Alkaloids isolation & purification, Antineoplastic Agents, Phytogenic pharmacology, Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic isolation & purification, Cell Proliferation drug effects, Drug Screening Assays, Antitumor, Piperidines pharmacology, Piperidines chemistry, Piperidines isolation & purification, Alocasia chemistry
- Abstract
Seventeen piperidine alkaloids, including 15 previously undescribed 2-substituted-6-(9-phenylnonyl)-piperidine-3,4-diol alkaloids and a previously undescribed 2-substituted-6-(9-phenylnonyl)-piperidine-3-ol alkaloid, were isolated from the leaves of Alocasia macrorrhiza (L.) Schott. Their planar structures and configurations were elucidated based on HR-ESI-MS, 1D and 2D NMR, Snatzke's method, modified Mosher method, single-crystal X-ray crystallography, as well as quantum chemical calculation. It was found that Δδ
H5b - H5a can be used to elucidate the relative configuration of 2,3,4,6-tetrasubstituted piperidine, by analyzing the NMR data of 2-substituted-6-(9-phenylnonyl)-piperidine-3,4-diol. Antiproliferative activity was evaluated for all of the alkaloids, and compounds 6-8 showed considerable inhibitory activity against K562 cell line, with the IC50 values of 17.24 ± 1.62, 19.31 ± 0.9 and 18.77 ± 1.09μM, respectively. Furthermore, compounds 6 and 7 exerted an antiproliferative effect by inducing apoptosis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
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12. Isoflavones isolated from the fruits of Ficus altissima and their anti-proliferative activities.
- Author
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Liu Y, Pu Y, Shen L, Li D, Xu J, He X, and Wang Y
- Subjects
- Humans, Molecular Structure, Cell Line, Tumor, China, Phytochemicals pharmacology, Phytochemicals isolation & purification, Cell Proliferation drug effects, K562 Cells, Ficus chemistry, Fruit chemistry, Isoflavones pharmacology, Isoflavones isolation & purification, Antineoplastic Agents, Phytogenic pharmacology, Antineoplastic Agents, Phytogenic isolation & purification, Apoptosis drug effects
- Abstract
Ficus altissima, also known as lofty fig, is a monoecious plant from the Moraceae family commonly found in southern China. In this study, we isolated and identified one new isoflavone (1), three new hydroxycoumaronochromones (2a, 2b and 3a) and 12 known compounds from the fruits of F. altissima. Their chemical structures were determined using spectroscopic analysis methods. We also tested all the isolated compounds for their anti-proliferative activities against eight human tumour cell lines (A-549, AGS, K562, K562/ADR, HepG2, HeLa, SPC-A-1 and CNE2) using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Our experiments showed that compound 6 exhibited obvious anti-proliferative activity against the K562 cell line with an IC
50 value of 1.55 μM. Additionally, compounds 8 and 9 showed significant anti-proliferative activities against the AGS and K562 cell lines, respectively. Moreover, compound 6 induced apoptosis in K562 cells through the caspase family signalling pathway., Competing Interests: Declaration of competing interest The authors declare no competing financial interest., (Copyright © 2023. Published by Elsevier B.V.)- Published
- 2024
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13. Anti-inflammatory monoterpenes from morinda (Morinda officinalis How.).
- Author
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Jiang Y, Chen L, Zeng J, Wang Y, Chen Y, Chen S, Xu J, and He X
- Subjects
- Health Promotion, Anti-Inflammatory Agents pharmacology, NF-kappa B metabolism, Iridoids pharmacology, Nitric Oxide, Lipopolysaccharides pharmacology, Nitric Oxide Synthase Type II metabolism, Morinda chemistry
- Abstract
Morinda (Morinda officinalis How.) is widely consumed as a functional food owing to its potential to promote health. This study investigated the anti-inflammatory phytochemicals of morinda and isolated 30 monoterpenes, including 6 undescribed iridoids (1, 6, 9-11 and 25), 2 undescribed acyclic monoterpenoids (28, 29), a known acyclic monoterpenoid and 21 known iridoids. Their chemical and stereo-structures were elucidated based on HR-ESI-MS, NMR,
13 C-NMR calculations, ECD data and ECD calculations. Notably, compounds 11, 12 and 20 exerted pronounced inhibitory effects on lipopolysaccharide-induced nitric oxide production in RAW 264.7 macrophages, with IC50 values of 28.51 ± 1.70, 25.45 ± 4.17 and 29.17 ± 3.71 μM respectively (indomethacin, IC50 of 33.68 ± 2.19 μM). The same compounds exert anti-inflammatory effects by blocking nuclear translocation of nuclear factor κ-B, and down-regulating the expression of inflammatory cytokines such as cyclooxygenase-2, inducible nitric oxide synthase, interleukin-1β and interleukin-6 at mRNA and protein levels in a dose-dependent manner. These results suggest that moderate consumption of morinda helps prevent and reduce the occurrence of inflammatory-related diseases., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)- Published
- 2024
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14. Associations of Various Physical Activities with Mortality and Life Expectancy are Mediated by Telomere Length.
- Author
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Zhou HH, Jin B, Liao Y, Hu Y, Li P, YangLha T, Liu Y, Xu J, Wang B, Zhu M, Xiao J, Liu J, Nüssler AK, Liu L, Hao X, Chen J, Peng Z, and Yang W
- Subjects
- Adult, Humans, Middle Aged, Prospective Studies, Longevity, Telomere, Exercise, Life Expectancy
- Abstract
Objectives: Physical activity (PA) and telomeres both contribute to healthy aging and longevity. To investigate the optimal dosage of various PA for longevity and the role of telomere length in PA and mortality., Design: Prospective cohort study., Setting and Participants: A total of 333,865 adults (mean age of 56 years) from the UK Biobank were analyzed., Methods: Walking, moderate PA (MPA), and vigorous PA (VPA) were self-reported via questionnaire, and leukocyte telomere length (LTL) was measured. Cox proportional hazards regression was used to predict all-cause mortality risk. A flexible parametric Royston-Parmar survival model was used to estimate life expectancy., Results: During a median follow-up of 13.8 years, 19,789 deaths were recorded. Compared with the no-walking group, 90 to 720 minutes/week of walking was similarly associated with 27% to 31% of lower mortality and about 6 years of additional life expectancy. We observed nearly major benefits for mortality and life expectancy among those meeting the PA guidelines [151-300 minutes/wk for MPA: hazard ratio (HR) 0.80, 95% CI 0.75-0.85, 3.40-3.42 additional life years; 76-150 minutes/wk for VPA: HR 0.78, 95% CI 0.75-0.82, 2.61 years (2.33-2.89)] vs the no-PA group. Similar benefits were also observed at 76-150 and 301-375 minutes/wk of MPA (18%-19% lower mortality, 3.20-3.42 gained years) or 151-300 minutes/wk of VPA (20%-26% lower mortality, 2.41-2.61 gained years). The associations between MPA, VPA, and mortality risk were slightly mediated by LTL (≈1% mediation proportion, both P < .001)., Conclusions and Implications: Our study suggests a more flexible range of PA than the current PA guidelines, which could gain similar benefits and is easier to achieve: 90 to 720 minutes/wk of walking, 75 to 375 minutes/wk of MPA, and 75 to 300 minutes/wk of VPA. Telomeres might be a potential mechanism by which PA promotes longevity., (Copyright © 2023 AMDA – The Society for Post-Acute and Long-Term Care Medicine. Published by Elsevier Inc. All rights reserved.)
- Published
- 2024
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15. Epidemiological characteristics and transmissibility of HPV infection: A long-term retrospective study in Hokkien Golden Triangle, China, 2013-2021.
- Author
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Xu J, Abudurusuli G, Rui J, Li Z, Zhao Z, Xia Y, Guo X, Abudunaibi B, Zhao B, Guo Q, Cui JA, Zhou Y, and Chen T
- Subjects
- Humans, Retrospective Studies, Human Papillomavirus Viruses, China epidemiology, Papillomavirus Infections epidemiology, Papillomavirus Infections genetics
- Abstract
Objective: Multiple human papillomavirus (HPV)-associated diseases have put a significant disease burden on the world. Therefore, we conducted a study to explore the epidemiological characteristics of HPV and the transmissibility of its genotypes., Methods: HPV testing data was collected from Hospital. A transmission dynamics model of HPV was constructed to simulate and compare the transmissibility of different HPV genotypes, which was quantitatively described by the basic reproduction number (R
0 )., Results: The collected HPV subjects were mainly from Xiamen City, Zhangzhou City and Quanzhou City, together, they are known as the Hokkien golden triangle. There were variations in the distribution of HPV infections by age groups. Among all HPV genotypes, 13 of them had R0 > 1, with 10 of them being high-risk types. The top five were HPV56, 18, 58, 52 and 53, among which, HPV56, 18, 58 and 42 were of high risk, whereas HPV53 was not, and the R0 values for the five were 3.35 (CI: 0.00-9.99), 3.20 (CI: 0.00-6.46), 3.19 (CI: 1.27-6.94), 3.19 (CI: 1.01-8.42) and 2.99 (CI: 0.00-9.39), respectively. In addition, HPV52 had R0 > 1 for about 51 months, which had the longest duration., Conclusion: Most high-risk HPV types in the Hokkien golden triangle could transmit among the population. Therefore, there is a need of further optimization for developing HPV vaccines and better detection methods in the region., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier B.V. All rights reserved.)- Published
- 2023
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16. Metal organic frameworks-in-nanochannels: A tailorable chromatography membrane for isolation of target protein.
- Author
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Xu J, Yang X, Guo J, Xu H, Gao Z, and Song YY
- Subjects
- Adsorption, Chromatography, Excipients, Metal-Organic Frameworks
- Abstract
Metal-organic frameworks (MOFs) demonstrate strong potential in biosample separation. However, the obtained MOFs powders are unsuitable for recovery techniques in an aqueous solution, especially the challenges of withdrawing MOFs particles and expanding their functions for specific applications. Herein, a general strategy is designed utilizing metal oxide-nanochannel arrays as precursors and templates for in-situ selective growth of MOFs structures. The exemplary MOFs (Ni-bipy) with tailored composition are selectively grown in NiO/TiO
2 nanochannel membrane (NM) using NiO as the sacrificial precursor, which enables one to achieve a ∼262 times concentration of histidine-tagged proteins within 100 min. The significantly improved adsorption efficiency in a wide pH range and the effective enrichment from a complex matrix as a nanofilter illustrate the great potential of MOFs in nanochannels membranes for the high-efficiency recovery of essential proteins in complex biological samples. The porous self-aligned Ni-MOFs/TiO2 NM exhibits biocompatibility and flexible functionalities, which is desirable for the generation of multifunctional nanofilter devices and developing biomacromolecule delivery vehicles., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)- Published
- 2023
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17. Catalytic probes based on aggregation-induced emission-active Au nanoclusters for visualizing MicroRNA in living cells and in vivo.
- Author
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Shu W, Zhang X, Tang H, Wang L, Cheng M, Xu J, Li R, and Ran X
- Subjects
- Animals, Manganese Compounds, Gold, Oxides, MicroRNAs, Metal Nanoparticles
- Abstract
Highly sensitive monitoring of cancer-related miRNAs is of great significance for tumor diagnosis. Herein, catalytic probes based on DNA-functionalized Au nanoclusters (AuNCs) were prepared in this work. The aggregation-induced emission-active Au nanoclusters showed an interesting phenomenon of aggregation induced emission (AIE) affected by the aggregation state. Leveraging this property, the AIE-active AuNCs were used to develop catalytic turn-on probes for detecting in vivo cancer-related miRNA based on a hybridization chain reaction (HCR). The target miRNA triggered the HCR and induced aggregation of AIE-active AuNCs, leading to a highly luminescent signal. The catalytic approach demonstrated a remarkable selectivity and a low detection limit in comparison to noncatalytic sensing signals. In addition, the excellent delivery the ability of MnO
2 carrier made it possible to use the probes for intracellular imaging and in vivo imaging. Effective in situ visualization of miR-21 was achieved not only in living cells but also in tumors in living animals. This approach potentially offers a novel method for obtaining information for tumor diagnosis via highly sensitive cancer-related miRNA imaging in vivo., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
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18. Anti-inflammatory iridoid glycosides from Paederia scandens (Lour.) Merrill.
- Author
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Xu Y, Zeng J, Wang L, Xu J, He X, and Wang Y
- Subjects
- Iridoids pharmacology, Iridoids chemistry, Plant Extracts pharmacology, Plant Extracts chemistry, NF-kappa B, Anti-Inflammatory Agents pharmacology, Lipopolysaccharides pharmacology, Nitric Oxide, Iridoid Glycosides pharmacology, Rubiaceae chemistry
- Abstract
Eight previously undescribed iridoid glycosides together with 20 known congeners were isolated from the aerial parts of Paederia scandens (Lour.) Merrill (Rubiaceae). Their structures incorporating absolute configurations were elucidated based on the comprehensive analyses of NMR data, HR-ESI-MS spectrometry, and ECD data. The potential anti-inflammatory activities of the isolated iridoids were evaluated in lipopolysaccharide-stimulated RAW 264.7 macrophages. Compound 6 significantly inhibited the production of nitric oxide with an IC
50 value of 15.30 μM. The results of immunoblotting, qPCR, and immunofluorescence staining assays revealed that compound 6 exhibited anti-inflammatory activity by suppressing nuclear translocation of NF-κB and reducing the expression of COX-2, iNOS, IL-1β, and IL-6. These results provide a basis for further development and utilization of P. scandens as a natural source of potential anti-inflammatory agents., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)- Published
- 2023
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19. Generation of an induced pluripotent stem cell (iPSC) line (INNDSUi002-A) from a patient with riboflavin-responsive multiple acyl-CoA dehydrogenase deficiency.
- Author
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Zhang C, Wang D, Wang Y, Sun P, Zhao D, Zhao X, Zhao Y, Liu F, Xu J, and Yan C
- Subjects
- Humans, Electron-Transferring Flavoproteins genetics, Electron-Transferring Flavoproteins metabolism, Mutation genetics, Riboflavin genetics, Riboflavin metabolism, Multiple Acyl Coenzyme A Dehydrogenase Deficiency genetics, Multiple Acyl Coenzyme A Dehydrogenase Deficiency metabolism, Induced Pluripotent Stem Cells metabolism, Iron-Sulfur Proteins genetics, Iron-Sulfur Proteins metabolism
- Abstract
Mutations in the ETFDH gene, encoding electron transfer flavoprotein dehydrogenase, have been identified to cause riboflavin-responsive multiple acyl-CoA dehydrogenase deficiency (RR-MADD) (Wen et al., 2010). We performed the generation and characterization of human induced pluripotent stem cell (iPSC) line from skin fibroblasts of a patient with RR-MADD carrying two heterozygous ETFDH mutations (p.D130V and p.A84V). Their pluripotency was verified by the expression of several pluripotency markers on RNA and protein levels and the capability to differentiate into all three germ layers., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier B.V.)
- Published
- 2023
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20. Anti-inflammatory steroids from the stems of Solanum nigrum L.
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Deng J, Wang L, Jin Q, Zeng J, Xu J, He X, and Wang Y
- Subjects
- Animals, Mice, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents metabolism, Macrophages metabolism, NF-kappa B metabolism, Steroids chemistry, Lipopolysaccharides pharmacology, Nitric Oxide, Nitric Oxide Synthase Type II metabolism, RAW 264.7 Cells, Solanum nigrum chemistry
- Abstract
Sixteen previously undescribed steroidal sapogenins along with two known ones were isolated from the stems of Solanum nigrum L. (Solanaceae). Their structures were identified using a combination of 1D and 2D NMR, HR-ESI-MS spectroscopy, the Mosher method, and X-ray diffraction analysis. Compounds 1-8 have an unusual F ring and 9-12 have a derived A ring, both of which are rare skeletons found in natural products. The biological evaluation showed that the isolated steroids exhibited inhibition of nitric oxide in the LPS-induced RAW 264.7 macrophages with IC
50 values from 7.4 to 41.3 μM. Further studies revealed that compounds 6 and 10 exhibited anti-inflammatory activity by blocking the nuclear translocation of NF-κB, and down-regulating the expression of iNOS, COX-2, IL-1β, and IL-6 in a concentration-dependent manner. These results suggest that the stems of S. nigrum may serve as a source of anti-inflammatory agents for use in healthy or medicinal products., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)- Published
- 2023
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21. Anti-inflammatory phenolics and phenylpropanoids from Praxelis clematidea.
- Author
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Liang S, Wang L, Xiong Z, Zeng J, Xiao L, Xu J, He X, and Wang Y
- Subjects
- NF-kappa B metabolism, Molecular Structure, Anti-Inflammatory Agents pharmacology, Plant Extracts chemistry, Nitric Oxide Synthase Type II metabolism, Lipopolysaccharides pharmacology, Nitric Oxide metabolism, Asteraceae chemistry
- Abstract
Praxelis clematidea (Asteraceae) is a noxious invasive exotic plant in southern China, and it has caused great damage to ecological conditions and serious financial losses. In this study, four new phenolics (1, 2, 7, 8), and two new phenylpropanoids (3, 4), along with seventeen known compounds were separated and purified from the whole plant of P. clematidea. Their chemical structures were determined by extensive spectroscopic analysis methods. Additionally, the potential inhibitory activities on nitric oxide (NO) production and NF-κB nuclear translocation in LPS-triggered RAW 264.7 macrophages of the isolated compounds were evaluated. Notably, compounds 2, 7, and 8 showed significant inhibitory activities on NO production, and also inhibited the expression of iNOS and COX-2. Furthermore, compounds 2, 7, and 8 effectively suppressed the NF-κB nuclear translocation. These findings suggest that P. clematidea has the potential to be developed and promoted as a treatment for inflammation-related diseases., Competing Interests: Declaration of Competing Interest The authors declare no competing financial interest., (Copyright © 2023 Elsevier B.V. All rights reserved.)
- Published
- 2023
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22. Anti-proliferative and anti-inflammatory eudesmanolides from the flowers of Sphagneticola trilobata (L.) Pruski.
- Author
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Zhang B, Wang L, Wang L, Wang Y, Xu J, and He X
- Subjects
- Humans, Cell Line, Tumor, Flowers chemistry, Magnetic Resonance Spectroscopy, Anti-Inflammatory Agents pharmacology, Anti-Inflammatory Agents chemistry, Asteraceae chemistry, Antineoplastic Agents
- Abstract
Twenty-six eudesmanolides including six undescribed compounds were isolated from the flowers of Sphagneticola trilobata (L.) Pruski. Their structures were elucidated based on the interpretation of spectroscopic techniques, NMR calculation, and DP4+ analysis. The stereochemistry of (1S,4S,5R,6S,7R,8S,9R,10S,11S)-1,4,8- trihydroxy-6-isobutyryloxy-11-methyleudesman-9,12-olide (1) was demonstrated by single crystal X-ray diffraction. All eudesmanolides were evaluated for their anti-proliferative activities against four human tumor cell lines (HepG2, HeLa, SGC-7901, and MCF-7). 1α,4β-Dihydroxy-6α-methacryloxy-8β-isobutyryloxyeudesman-9,12-olide (3) and wedelolide B (8) showed pronounced cytotoxic effects against AGS cell line with IC
50 values of 1.31 and 0.89 μM, respectively. Their anti-proliferative activities against AGS cells were exerted through a dose-dependent apoptosis pathway, as verified by cell and nucleus morphological assessment, clone formation assay, and Western blot analysis. Furthermore, 1α,4β,8β-trihydroxy-6β-methacryloxyeudesman-9,12-olide (2) and 1α,4β,9β-trihydroxy-6α-isobutyryloxy- 11α-13-methacryloxyprostatolide (7) performed significant inhibitory effects on lipopolysaccharide-stimulated nitric oxide production in RAW 264.7 macrophages with IC50 values of 11.82 and 11.05 μM, respectively. Moreover, compounds 2 and 7 could block the nuclear translocation of NF-κB and reduce the expression of iNOS, COX-2, IL-1β, and IL-6 to exert anti-inflammatory effects. This study provides evidence for the utilization of the eudesmanolides from S. trilobata as lead compounds for further research due to their cytotoxic potential., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)- Published
- 2023
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23. Therapeutic potential of kaempferol on Streptococcus pneumoniae infection.
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Xu L, Fang J, Ou D, Xu J, Deng X, Chi G, Feng H, and Wang J
- Subjects
- Humans, Kaempferols pharmacology, Kaempferols therapeutic use, SARS-CoV-2, Streptococcus pneumoniae, Virulence Factors, Bacterial Proteins, Coinfection, COVID-19, Pneumococcal Infections drug therapy, Pneumococcal Infections microbiology
- Abstract
Co-infections with pathogens and secondary bacterial infections play significant roles during the pandemic coronavirus disease 2019 (COVID-19) pathogenetic process, caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). Notably, co-infections with Streptococcus pneumoniae (S. pneumoniae), as a major Gram-positive pathogen causing pneumonia or meningitis, severely threaten the diagnosis, therapy, and prognosis of COVID-19 worldwide. Accumulating evidences have emerged indicating that S. pneumoniae evolves multiple virulence factors, including pneumolysin (PLY) and sortase A (SrtA), which have been extensively explored as alternative anti-infection targets. In our study, natural flavonoid kaempferol was identified as a potential candidate drug for infection therapeutics via anti-virulence mechanisms. We found that kaempferol could interfere with the pore-forming activity of PLY by engaging with catalytic active sites and consequently inhibit PLY-mediated cytotoxicity. Additionally, exposed to kaempferol significantly reduced the SrtA peptidase activity by occupying the active sites of SrtA. Further, the biofilms formation and bacterial adhesion to the host cells could be significantly thwarted by kaempferol incubation. In vivo infection model by S. pneumoniae highlighted that kaempferol oral administration exhibited notable treatment benefits, as evidenced by decreased bacterial burden, suggesting that kaempferol has tremendous potential to attenuate S. pneumoniae pathogenicity. Scientifically, our study implies that kaempferol is a promising therapeutic option by targeting bacterial virulence factors., Competing Interests: Declaration of competing interest All authors declare no conflicts of interest., (Copyright © 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
- Published
- 2023
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24. Enhancement of sugar release from sugarcane bagasse through NaOH-catalyzed ethylene glycol pretreatment and water-soluble sulfonated lignin.
- Author
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Wang B, Qi J, Xie M, Wang X, Xu J, Yu Z, Zhao W, Xiao Y, and Wei W
- Subjects
- Cellulose, Lignin, Sodium Hydroxide, Sugars, Ethylene Glycol, Water, Hydrolysis, Catalysis, Glucose, Saccharum, Cellulase
- Abstract
In this work, five different NaOH-catalyzed ethylene glycol (EG) pretreatments together with water-soluble sulfonated lignin (SL) were used for enhancing sugarcane bagasse (SCB) enzymatic digestion. The results showed that the coupling of NaOH and EG into a one-pot pretreatment (10%NaOH/EG) was more beneficial to improve SCB enzymatic hydrolysis than that of single 10%NaOH or EG pretreatment, or the two-step pretreatment of NaOH and EG in different sequence (10%NaOH+EG and EG + 10%NaOH, respectively). The highest glucose yield of this work was 91.2 %, mainly released from the SCB that pretreated with 10%NaOH/EG at 130 °C for 60 min and 72 h enzymatic hydrolysis. The adding of SL into the enzymatic hydrolysis step could significantly lower the cellulase dosage and hydrolysis time from 20 FPU/g and 72 h to 10 FPU/g and 24 h, respectively, meanwhile keeping a high glucose yield of 90.4 %. The characterization of various pretreated or un-pretreated SCB confirmed that the improvement of hydrolysis efficiency of SCB after 10%NaOH/EG pretreatment was closely related to the removal of various components barriers in SCB and the fragmentation of pretreated solid. It can be concluded that the developed NaOH-catalyzed ethylene glycol pretreatment was an efficiency way to enhance the sugar release from SCB., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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25. Anti-neuroinflammatory 3-hydroxycoumaronochromones and isoflavanones enantiomers from the fruits of Ficus altissima Blume.
- Author
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Yao J, Qin Q, Wang Y, Zeng J, Xu J, and He X
- Subjects
- Anti-Inflammatory Agents chemistry, Fruit metabolism, Isoflavones, Lipopolysaccharides pharmacology, Microglia metabolism, NF-kappa B metabolism, Nitric Oxide metabolism, Ficus metabolism
- Abstract
A phytochemical study on the fruits of Ficus altissima Blume (lofty fig) led to the isolation and structural elucidation of three pairs of enantiomeric 3-hydroxycoumaronochromones and two pairs of enantiomeric isoflavanones, including eight undescribed compounds. Their structures were determined based on a comprehensive analysis of NMR and HR-ESI-MS spectroscopic data, calculated
13 C NMR-DP4 plus analysis and the comparisons of experimental measurements of ECD with calculated ECD spectra by TDDFT or ECD plots in reported protocols. The inhibitory effects of the isolated enantiomers on NO production stimulated by LPS in microglial BV-2 cells were evaluated. Among them, ficusaltin D exhibited the most potent anti-neuroinflammatory activity, which inhibited the production of NO and the expression of iNOS, IL-6 and IL-1β and suppressed the NF-κB nuclear translocation in LPS-induced BV-2 cells, while its enantiomer displayed cytotoxicity., (Copyright © 2022 Elsevier Ltd. All rights reserved.)- Published
- 2022
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26. The Annexin A2-Notch regulatory loop in hepatocytes promotes liver fibrosis in NAFLD by increasing osteopontin expression.
- Author
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Wang G, Duan J, Pu G, Ye C, Li Y, Xiu W, Xu J, Liu B, Zhu Y, and Wang C
- Subjects
- Animals, Hepatocytes metabolism, Humans, Liver Cirrhosis pathology, Methionine metabolism, Mice, Mice, Inbred C57BL, Annexin A2 genetics, Annexin A2 metabolism, Non-alcoholic Fatty Liver Disease pathology, Osteopontin genetics, Osteopontin metabolism
- Abstract
Background: The mechanisms underlying the progression of liver disease from simple hepatic steatosis to advanced nonalcoholic steatohepatitis (NASH) and liver fibrosis warrant further investigation. Increased mRNA levels of Annexin A2 protein (Anxa2) have been observed in patients with NASH. However, the role of Anxa2 in NASH remains unclear., Methods: The protein levels of Anxa2 were analyzed in the livers of mice and patients with NASH. Anxa2-knockout and -knockdown mice were generated, and NASH was induced through a high fructose, palmitate, and cholesterol (FPC) diet or methionine- and choline-deficient (MCD) diet., Findings: We found elevated expression of Anxa2 in the livers of patients and mice with NASH. Anxa2 knockdown but not knockout ameliorated liver fibrosis in both FPC and MCD diet-fed mice. Liver-specific Anxa2 overexpression increased collagen deposition in mice fed a normal diet. Mechanistically, Anxa2 overexpression in hepatocytes promoted hepatic stellate cell activation in a paracrine manner by increasing osteopontin expression. Notch inhibition suppressed the exogenous overexpression of Anxa2-induced osteopontin and endogenous Anxa2 expression. Additionally, Anxa2 overexpression accelerated the progression of nonalcoholic fatty liver disease (NAFLD) in mice fed a high-fat diet. Moreover, Anxa2 levels were higher in NAFLD patients with advanced liver fibrosis than in those with mild liver fibrosis, as determined using the Gene Expression Omnibus database., Interpretation: In conclusion, we found increased Anxa2 expression in hepatocytes promoted liver fibrosis in NASH mice by increasing osteopontin expression. The Anxa2-Notch positive regulatory loop contributes to this process and represents a novel target for the treatment of NASH-related liver fibrosis., (Copyright © 2022. Published by Elsevier B.V.)
- Published
- 2022
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27. Establishment of an induced pluripotent stem cell (iPSC) line (INNDSUi001-A) from a healthy female Chinese Han.
- Author
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Wang D, Zhang R, Wang Y, Sun P, Ji X, Sun X, Xu J, Zhao D, Yan C, and Liu F
- Subjects
- Adult, Cell Differentiation, Cellular Reprogramming, China, Female, Fibroblasts metabolism, Germ Layers, Humans, Young Adult, Induced Pluripotent Stem Cells metabolism
- Abstract
We have established the human induced pluripotent stem cell (hiPSC) line (INNDSUi001-A) from skin fibroblasts of a healthy 24-year-old female individual by using non-integrating reprogramming method. The resulted cells had typical features of embryonic stem cell as indicated by expression of specific pluripotency markers and had the capability of in vitro differentiation into the three germ layers. This iPSC line can be used as a healthy control for disease study., (Copyright © 2022. Published by Elsevier B.V.)
- Published
- 2022
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28. Generation of a human induced pluripotent stem cell line (INNDSUi003-A) derived from patient with Becker muscular dystrophy (BMD).
- Author
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Ji X, Wang Y, Sun P, Wang D, Sun X, Zhang R, Xu J, Li W, Liu F, and Yan C
- Subjects
- Fibroblasts pathology, Humans, Mutation, Induced Pluripotent Stem Cells metabolism, Muscular Dystrophy, Duchenne pathology
- Abstract
Becker muscular dystrophy (BMD), an X-linked recessive disorder caused of mutation in the dystrophin gene, is characterized by progressive muscle degeneration and proximal muscle weakness. We generated a human induced pluripotent stem cell (hiPSC) line from the fibroblasts isolated from patient with BMD by non-integrating reprogramming methods. The iPSC line highly expresses pluripotency markers, displays the normal karyotype and is able to differentiate into the three germ layers in vitro. The iPSC line will be a useful tool to study the pathogenesis of BMD and for drug screening., (Copyright © 2022 The Authors. Published by Elsevier B.V. All rights reserved.)
- Published
- 2022
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29. Structure characterization, antioxidant and emulsifying capacities of exopolysaccharide derived from Tetragenococcus halophilus SNTH-8.
- Author
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Yang X, Wu J, An F, Xu J, Bat-Ochir M, Wei L, Li M, Bilige M, and Wu R
- Subjects
- Enterococcaceae, Spectroscopy, Fourier Transform Infrared, Antioxidants chemistry, Polysaccharides chemistry
- Abstract
Tetragenococcus halophilus exopolysaccharides (THPS) are metabolites released by T. halophilus SNTH-8 to resist a high-salt environment. Although many studies have investigated the mechanisms underlying salt tolerance shown by T. halophilus, structural characteristics as well as antioxidant and emulsifying capacities of THPS remain unclear. In this study, we isolated and purified two components, THPS-1 and THPS-2, from T. halophilus SNTH-8. Purified THPS-1 and THPS-2 were composed of arabinose, xylose, fucose, galactose, glucose, and glucuronic acid at a molar ratio of 1.66:38.95:2.11:26.12:29.73:1.43 and 0.46:40.3:0.54:30.8:1.36:25.54, respectively. The average molecular weights of THPS-1 and THPS-2 were 14.98 kDa and 21.03 kDa, respectively. Moreover, the structures of THPS-1 and THPS-2 were investigated via fourier-transform infrared spectroscopy(FT-IR), nuclear magnetic resonance spectroscopy(NMR), scanning electron microscopy(SEM), and methylation analysis. THPS-1 was a highly branched polysaccharide with a backbone of α-D-(1,4)-Xyl, α-D-(1,6)-Glc and α-D-Xyl as the terminal, while THPS-2 was a highly branched polysaccharide with a backbone of α-D-(1,4)-Xyl and β-D-GlcA as the terminal. The branches were identified as β-D-(1,4,6)-Gal and β-D-(1,6)-Gal. Both THPS-1 and THPS-2 exhibited high antioxidant and emulsifying capacities. Overall, our structural analysis of THPS may further enhance research on natural emulsifiers and antioxidants., (Copyright © 2022. Published by Elsevier B.V.)
- Published
- 2022
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30. Antiproliferative Amaryllidaceae alkaloids from the bulbs of Hymenocallis littoralis (Jacq.) Salisb.
- Author
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Ma W, Wang S, Wang Y, Zeng J, Xu J, and He X
- Subjects
- Plant Extracts pharmacology, Plant Roots chemistry, Alkaloids analysis, Alkaloids pharmacology, Amaryllidaceae Alkaloids chemistry, Amaryllidaceae Alkaloids pharmacology, Liliaceae chemistry
- Abstract
Seven undescribed Amaryllidaceae alkaloids classified into four types, including the plicamine-type, secoplicamine-type, belladine-type and pretazettine-type, along with another three alkaloids that have not been isolated from plant material and seven known alkaloids, were isolated from the bulbs of Hymenocallis littoralis (Jacq.) Salisb. The structures were elucidated on the basis of various spectroscopic methods (UV, IR, MS, NMR, ECD). The isolated alkaloids were screened for antiproliferative activity against four human tumour cell lines (HepG2, HeLa, SPC-A-1, FaDu) through MTT assay, and some alkaloids exhibited potent cytotoxicity. Meanwhile, cell morphological assessment, flow cytometric analysis, Western blot analysis, clone formation and scratch wound assays were utilized for an undescribed belladine-type alkaloid and two known alkaloids, which had antiproliferative effects on the HepG2 cell line via induction of apoptosis in a dose-dependent manner. A pair of diastereoisomers of Amaryllidaceae alkaloids exhibited significant differences in antiproliferative activity. In addition, the alkaloids also possessed the potential to inhibit tumour cell migration., (Copyright © 2022 Elsevier Ltd. All rights reserved.)
- Published
- 2022
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31. A-24, a steroidal saponin from Allium chinense, induced apoptosis, autophagy and migration inhibition in p53 wild-type and p53-deficient gastric cancer cells.
- Author
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Xu J, Wang Y, Wang Y, Wang Z, and He X
- Subjects
- Humans, Cell Line, Tumor, Cell Movement drug effects, Cell Proliferation drug effects, Phosphatidylinositol 3-Kinases metabolism, Signal Transduction drug effects, Allium chemistry, Apoptosis drug effects, Autophagy drug effects, Proto-Oncogene Proteins c-akt metabolism, Reactive Oxygen Species metabolism, Saponins pharmacology, Stomach Neoplasms pathology, Stomach Neoplasms drug therapy, Stomach Neoplasms metabolism, TOR Serine-Threonine Kinases metabolism, Tumor Suppressor Protein p53 metabolism, Tumor Suppressor Protein p53 deficiency, Tumor Suppressor Protein p53 genetics
- Abstract
Allium chinense is a vegetable with nutrition and unique flavor, and it is used as traditional Chinese medicine. We previously reported that the active compound A-24 induces apoptosis and autophagy in p53 wild-type gastric cancer cells through the PI3K/Akt/mTOR pathway. Our present work indicates that A-24 also has a significant proliferation inhibition effect on p53-deficient KATO-III cells, and the p53 status did not affect A-24 induced migration inhibition, but negatively controlled the occurrence of autophagy. We also found that the accumulation of reactive oxygen species (ROS) mediated A-24 induced apoptosis is p53-independent. Besides, p-Akt was not downregulated by A-24 in p53-deficient gastric cancer cells. Taken together, our results indicate that A-24 induced apoptosis and autophagy via the ROS-PI3K/Akt/mTOR pathway in p53 wild-type gastric cancer cells and through the ROS-mTOR pathway in p53-deficient gastric cancer cells. Our study recommended A-24 as a promising future phytotherapeutic candidate for gastric cancer treatment., (Copyright © 2021 Elsevier B.V. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
32. Anti-proliferative and anti-inflammatory prenylated isoflavones and coumaronochromones from the fruits of Ficus altissima.
- Author
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Yao J, Wang Z, Wang R, Wang Y, Xu J, and He X
- Subjects
- Animals, Anti-Inflammatory Agents, Non-Steroidal chemistry, Anti-Inflammatory Agents, Non-Steroidal isolation & purification, Antineoplastic Agents, Phytogenic chemistry, Antineoplastic Agents, Phytogenic isolation & purification, Apoptosis drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Cell Survival drug effects, Chromones chemistry, Chromones isolation & purification, Coumarins chemistry, Coumarins isolation & purification, Dose-Response Relationship, Drug, Drug Screening Assays, Antitumor, Humans, Isoflavones chemistry, Isoflavones isolation & purification, Lipopolysaccharides antagonists & inhibitors, Lipopolysaccharides pharmacology, Mice, Molecular Structure, Nitric Oxide antagonists & inhibitors, Nitric Oxide biosynthesis, RAW 264.7 Cells, Structure-Activity Relationship, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Antineoplastic Agents, Phytogenic pharmacology, Chromones pharmacology, Coumarins pharmacology, Ficus chemistry, Fruit chemistry, Isoflavones pharmacology
- Abstract
Ficus altissima, an evergreen arbor belonging to the Moraceae family, is mainly cultivated in the tropics and subtropics of South and Southeast Asia with the characteristic of exuberant vitality and luxuriant foliage. In this article, four new prenylated isoflavones (1-4), along with ten previously described isoflavones (5-14) and two known prenylated coumaronochromones (15 and 16) were firstly obtained from the fruits of F. altissima. Their structures were identified by various spectroscopic techniques including specific optical rotation, HR-ESI-MS and NMR. The isolated products were evaluated for their anti-proliferative activities against three human tumor cell lines (HepG2, MCF-7 and MDA-MB-231) through MTT assay. Compounds 2, 3 and 16 exhibited obvious anti-proliferative activities against MDA-MB-231 cell line and compounds 3, 13 and 16 showed effective cytotoxic effects on HepG2 cell line in a concentration-dependent manner, as verified by the colony formation assay, cell and nucleus morphological assessment and apoptosis assay. Meanwhile, compounds 5 and 12 exhibited significant inhibition activities on NO production in LPS-stimulated RAW 264.7 cell line compared with positive control indometacin. The phytochemical investigation of the fruits of F. altissima in this study could provide the evidence for the discovery of lead compounds., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
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33. Pronounced anti-neuroinflammatory jasmonates and terpenes isolated from lychee seeds.
- Author
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Zhang X, Wang Y, Qin Q, Wang Y, Xu J, and He X
- Subjects
- Animals, Anti-Inflammatory Agents isolation & purification, Cell Line, Cyclopentanes isolation & purification, Mice, Molecular Structure, Neuroprotective Agents isolation & purification, Nitric Oxide metabolism, Oxylipins isolation & purification, Phytochemicals isolation & purification, Phytochemicals pharmacology, Seeds chemistry, Terpenes isolation & purification, Anti-Inflammatory Agents pharmacology, Cyclopentanes pharmacology, Litchi chemistry, Microglia drug effects, Neuroprotective Agents pharmacology, Oxylipins pharmacology, Terpenes pharmacology
- Abstract
Lychee is a favorite fruit of the Cantonese and native to Southeast Asia. In this study, the anti-neuroinflammatory bioactive compounds of lychee seeds have been carried out. Five new jasmonates (1, 2, 6-8) and seventeen known compounds were isolated using a series of chemical and chromatographic methods. Their chemical structures were identified through comprehensive spectroscopic analysis. Anti-neuroinflammatory activities were assayed and evaluated for the purified compounds. Most of the compounds exhibited pronounced anti-neuroinflammatory activities on nitric oxide (NO) induced by lipopolysaccharide (LPS) in BV-2 microglia cells. Moreover, compounds 1, 2 and 20 could reduce the expression of LPS-induced pro-inflammatory factors (iNOS and COX-2), inhibit the expression of mRNA levels of iNOS, COX-2, IL-6 and block NF-κB nuclear translocation in dose-dependent manners. This study suggested that lychee phytochemicals could be benefit to some neuroinflammatory-associated diseases, such as Alzheimer's disease., (Copyright © 2021. Published by Elsevier B.V.)
- Published
- 2021
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34. T-17, a spirostanol saponin, inhibits p53-independent proliferation and p53-dependent migration of gastric cancer cells.
- Author
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Xu J, Wang Y, Wang Z, Wang Y, and He X
- Subjects
- Humans, Cell Line, Tumor, Spirostans pharmacology, Spirostans chemistry, Spirostans isolation & purification, Autophagy drug effects, Reactive Oxygen Species metabolism, HMGB1 Protein metabolism, HMGB1 Protein genetics, Tumor Suppressor Protein p53 metabolism, Tumor Suppressor Protein p53 genetics, Stomach Neoplasms pathology, Stomach Neoplasms metabolism, Stomach Neoplasms drug therapy, Saponins pharmacology, Saponins chemistry, Saponins isolation & purification, Cell Movement drug effects, Cell Proliferation drug effects, Apoptosis drug effects
- Abstract
The p53 tumor suppressor gene contributes to a series of life processes of cells. Previously, we have shown that T-17, a spirostanol saponin extracted from Tupistra chinensis induces cell cycle arrest, apoptosis and autophagy in gastric cancer cells. The p53 is essential in the cell cycle arrest induced by T-17, however, the effect of p53 on T-17-induced apoptosis and autophagy is still unclear. Here, our study shows that T-17 has no difference in the sensitivity of gastric cancer cells with different p53 status. By transfecting p53 siRNA into AGS cells (p53 wild type cells) or wild-type p53 into KATO-III cells (p53 deficiency cells), T-17 was found to induce apoptosis and autophagy in gastric cancer cells in a p53-independent manner. Pre-treatment with N-acetylcysteine (NAC, a ROS scavenger) demonstrated that reactive oxygen species (ROS) mediated T-17-induced p53-independent apoptosis. Besides, T-17 induces apoptosis and autophagy in gastric cancer cells by decreasing the expression of HMGB1, also in a p53-independent manner. But when we detected the inhibitory effect of T-17 on gastric cancer cell migration, it was found that p53 is essential. These experimental results showed that T-17 induced apoptosis and autophagy in gastric cancer cells in a p53-independent manner, but inhibited the migration of gastric cancer cells in a p53-dependent manner. Our research indicates that T-17 is a potential candidate for gastric cancer and provides support for better utilization of Tupistra chinensis., (Copyright © 2021 Elsevier Inc. All rights reserved.)
- Published
- 2021
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35. Anti-inflammatory naphthoates and anthraquinones from the roots of Morinda officinalis.
- Author
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Luo H, Wang Y, Qin Q, Wang Y, Xu J, and He X
- Subjects
- Animals, Anthraquinones chemistry, Anthraquinones isolation & purification, Anti-Inflammatory Agents, Non-Steroidal chemistry, Anti-Inflammatory Agents, Non-Steroidal isolation & purification, Dose-Response Relationship, Drug, Lipopolysaccharides antagonists & inhibitors, Lipopolysaccharides pharmacology, Mice, Molecular Structure, Naphthalenes chemistry, Naphthalenes isolation & purification, Nitric Oxide biosynthesis, RAW 264.7 Cells, Structure-Activity Relationship, Anthraquinones pharmacology, Anti-Inflammatory Agents, Non-Steroidal pharmacology, Morinda chemistry, Naphthalenes pharmacology, Nitric Oxide antagonists & inhibitors, Plant Roots chemistry
- Abstract
Morinda (Morinda officinalis) is widely consumed as a health-care herb in Asia and reported to possess various biological activities. In this study, anti-inflammatory phytochemicals were investigated and two pairs of new methyl-2-naphthoate enantiomers (1a/1b, 2a/2b), one new anthraquinone (3), three new natural unknown anthraquinones (5-6, 23), and eighteen known anthraquinones were isolated and elucidated from the roots of morinda. Anti-inflammatory activities of the isolated compounds were assessed in lipopolysaccharide-stimulated RAW 264.7 macrophages. Compounds 2b and 19 significantly inhibited the production of NO with IC
50 values of 34.32 ± 4.87 and 17.17 ± 4.13 μM (indomethacin, IC50 26.71 ± 6.32 μM), and they were further corroborated via immunoblotting, quantitative real-time PCR and immunofluorescence staining assays. They could dose-dependent suppress lipopolysaccharide-stimulated pro-inflammatory factors (COX-2 and iNOS) production and block nuclear translocation of NF-κB. The results implied that reasonable consumption of morinda may be beneficial for preventing and reducing the occurrence of inflammatory-associated diseases., (Copyright © 2021 Elsevier Inc. All rights reserved.)- Published
- 2021
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- View/download PDF
36. Renewable photoelectrochemical cytosensing platform for rapid capture and detection of circulating tumor cells.
- Author
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Xu J, Zhao C, Niu K, Gao Z, and Song YY
- Subjects
- Cell Count, Electrodes, Humans, MCF-7 Cells, Biosensing Techniques, Neoplastic Cells, Circulating
- Abstract
Determination of circulating tumor cells (CTCs) is crucial for cancer diagnosis and therapy at an early stage. However, extremely low concentration of CTCs in peripheral blood makes the detection of CTCs challenging. In this study, a reusable cytosensor was developed for rapid detection of CTCs based on excellent photoelectrochemical (PEC) characteristic of semiconductor nanoarrays. Using typical breast cancer cell, MCF-7 cell, as a target model, a PEC sensing platform was constructed with polymerized aminophenylboronic acid (APBA) layer coated CdS/ZnO nanorod arrays, exhibiting outstanding performance for the capture and detection of CTCs. In this design, the polymerized APBA provides abundant binding sites for capturing terminal sialic acid (SA) molecules in CTCs. As a result, the PEC cytosensor shows good sensitivity and specificity with concentrations ranging from 50 to 1.0 × 10
6 cells/mL MCF-7 cells. Moreover, the PEC cytosensor can be rapidly and effectively recovered via a short-time bias triggered cell release and subsequent repair of APBA. This study establishes a new approach to refine a PEC cytosensor for stable monitoring and provides a robust PEC electrode with high sensitivity and low cost for clinical diagnosis related to CTCs., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2021
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37. A spirostanol saponin isolated from Tupistra chinensis Baker simultaneously induces apoptosis and autophagy by regulating the JNK pathway in human gastric cancer cells.
- Author
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Xu J, Wang Z, Huang Y, Wang Y, Xiang L, and He X
- Subjects
- Cell Cycle Checkpoints drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Humans, Saponins isolation & purification, Stomach Neoplasms enzymology, Apoptosis drug effects, Asparagaceae chemistry, Autophagy drug effects, MAP Kinase Signaling System drug effects, Saponins pharmacology, Stomach Neoplasms pathology
- Abstract
T-17, a bioactive spirostanol saponin extracted from Tupistra chinensis Baker, was previously reported with anti-inflammatory and cytotoxic activities. However, the mechanism underlying of its anti-proliferation activity remains to be elucidated. In this study, we investigated the anti-gastric cancer cell growth activity of T-17 in terms of cell viability, colony formation, cell cycle, induction of apoptosis/autophagy, and JNK pathway. T-17 showed dose-dependent cytotoxicity in SGC-7901 and AGS cell lines, it induced caspase-mediated apoptosis as well as G0/G1 phase arrest and modulation of cyclinE2 and p21 expression. In addition, T-17 promoted the cancer cell autophagy as evidenced with increased expression of Beclin-1 and decreased p62 in western blot and formation of GFP-LC3 puncta. Furthermore, T-17-induced autophagy decreased gastric cancer cell apoptosis as assessed by pharmacological autophagy inhibitors and ATG5 siRNA usage. Importantly, the activation of JNK pathway was simultaneously involved in T-17-induced apoptosis and autophagy. Taken together, the results suggest that T-17 is a promising cytotoxic agent for therapeutic treatment of human gastric adenocarcinoma, which provides a good foundation for further research and development of Tupistra chinensis Baker., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
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38. Jasmonates from Chinese acorns (Quercus serrata var. brevipetiolata) exert pronounced anti-neuroinflammatory activities.
- Author
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Wang Y, Lei Y, Huang Y, Wang Z, Xu J, and He X
- Subjects
- Anti-Inflammatory Agents pharmacology, Humans, Inflammation Mediators pharmacology, Molecular Structure, Structure-Activity Relationship, Anti-Inflammatory Agents therapeutic use, Cyclopentanes chemistry, Cyclopentanes therapeutic use, Inflammation drug therapy, Inflammation Mediators therapeutic use, NF-kappa B metabolism, Oxylipins chemistry, Oxylipins therapeutic use, Quercus chemistry
- Abstract
Chinese oak (Quercus serrata var. brevipetiolata) belongs to the genus Quercus in Fagaceae family. Its seed, called as Chinese acorn, has been served as a traditional medicine and foodstuff in China. In this study, ten jasmonates were isolated and purified from Chinese acorn, including five new (1-5) and five known jasmonates (6-10). The new jasmonates were identified as butyl (1R,2R)-2-[(2'Z)-5'-hydroxy-penten-2'-enyl]-3-oxo-cyclopentane acetate (1), methyl {2-[4'-(β-d-glucopyranosyloxy)-pentyl}-3-oxo-cyclopentane acetate (2), methyl {(1R,2R)-2-[(2'Z,4'R)-4'-(β-d-glucopyransyloxy)-pent-2'-enyl]}-3-oxo-cyclopentane acetate (3), methyl {(1R,2R)-2-[(2'E,4'S)-4'-(β-d-glucopyransyloxy)-pent-2'-enyl]}-3-oxo-cyclopentane acetate (4), and methyl {(1R,2R)-2-[(2'S,3'E)-2'-(β-D-glucopyransyloxy)-pent-3'-enyl]}-3-oxo-cyclopentane acetate (5), respectively. The isolated jasmonates were evaluated for anti-neuroinflammatory activity, and some showed pronounced inhibitory effects on the production of nitric oxide (NO) induced by lipopolysaccharide (LPS) in BV-2 microglia cells. Some jasmonates could dose-dependently reduce the expression of LPS-induced pro-inflammatory factors (iNOS and COX-2) and could block NF-κB nuclear translocation. This study suggested that Chinese acorns could be served as a healthy product for neuroinflammatory related diseases, such as Alzheimer's disease., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
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39. A steroidal saponin isolated from Allium chinense simultaneously induces apoptosis and autophagy by modulating the PI3K/Akt/mTOR signaling pathway in human gastric adenocarcinoma.
- Author
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Xu J, Zhang M, Lin X, Wang Y, and He X
- Subjects
- Antineoplastic Agents chemistry, Antineoplastic Agents isolation & purification, Antineoplastic Agents pharmacology, Caspases metabolism, Cell Line, Tumor, Cell Proliferation drug effects, Enzyme Activation drug effects, G2 Phase Cell Cycle Checkpoints drug effects, Gene Expression Regulation, Neoplastic drug effects, Humans, M Phase Cell Cycle Checkpoints drug effects, Phosphatidylinositol 3-Kinases metabolism, Proto-Oncogene Proteins c-akt metabolism, Saponins chemistry, Saponins isolation & purification, Steroids chemistry, TOR Serine-Threonine Kinases metabolism, Adenocarcinoma pathology, Allium chemistry, Apoptosis drug effects, Autophagy drug effects, Saponins pharmacology, Signal Transduction drug effects, Stomach Neoplasms pathology
- Abstract
Allium chinense, as a side dish on Asian table, is often used in folk medicine for its health benefits. (25R)-5α-spirostan-3β-yl-3-O-acetyl-O-β-d-glucopyranosyl-(1 → 2)-O-[β-d-glucopyranosyl-(1 → 3)]-O-β-d-glucopyranosyl-(1 → 4)-β-d-galactopyranoside (A-24) is a bioactive steroidal saponin isolated from Allium chinense. Previously, we have shown that A-24 has cytotoxic effects on cancer cells, but not on normal cells. To further explore the underlying mechanisms, in this study, we investigated the anticancer activity of A-24 in human gastric cancer cell lines in terms of cell proliferation, colony formation, cell cycle, induction of apoptosis/autophagy, and PI3K/Akt/mTOR pathway. A-24 showed dose-dependent cytotoxicity in SGC-7901 and AGS cell lines, it induced intrinsic mitochondrial pathway of apoptosis as well as autophagy, G2/M phase arrest and modulation of cyclinB1, p-cdc2, p-wee1 and p-Histone H3 expression. Furthermore, A-24 downregulated the phosphorylation of Akt at Ser473 and mTOR at Ser2448 in PI3K/Akt/mTOR pathway, and its downstream substrates p-p70S6K and p-4EBP1 in a dose-dependent manner. In addition, the pre-treatment of tumor cells with 3-methyladenine (3-MA) and LY294002 increased A-24-induced apoptosis. Collectively, these findings highlight the significance of downregulation of PI3K/Akt/mTOR pathway in A-24-induced apoptosis and autophagy, and the potential application of A-24 as a novel candidate in the treatment of human gastric adenocarcinoma., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier Inc. All rights reserved.)
- Published
- 2020
- Full Text
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40. New anti-neuroinflammatory steroids against LPS induced NO production in BV2 microglia cells by microbial transformation of isorhodeasapogenin.
- Author
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Wang Y, Xiang L, Wang Z, Li J, Xu J, and He X
- Subjects
- Animals, Anti-Inflammatory Agents chemistry, Biotransformation, Catalysis, Cell Line, Cyclooxygenase 2 metabolism, Hydroxylation, Microglia metabolism, Molecular Structure, Nervous System metabolism, Nitric Oxide Synthase Type II metabolism, Organic Chemicals chemistry, Saponins chemistry, Spectrum Analysis methods, Steroids chemistry, Anti-Inflammatory Agents pharmacology, Lipopolysaccharides pharmacology, Microglia drug effects, Mucorales metabolism, Nervous System drug effects, Nitric Oxide biosynthesis, Organic Chemicals pharmacology, Saponins pharmacology, Steroids pharmacology
- Abstract
Microbial transformation of isorhodeasapogenin (1), the major steroidal sapogenin of Tupistra chinensis, was performed with the fungus Syncephalastrum racemosum (AS 3.264). As a result, nine new biotransformation metabolites (2-10) were isolated and their structures were elucidated by spectroscopic analysis. Hydroxylation, oxidation and glycosylation reactions were observed on the B, C, D and F rings of steroidal skeleton. Substrate (1) and its biotransformed metabolites 2-6, 8-10 were evaluated for their anti-neuroinflammatory effect on the NO accumulation induced by LPS in BV-2 cells. All the tested metabolites were found to have more potential anti-neuroinflammatory activity than the substrate. Especially, metabolites 2, 5 and 6 exhibited significant inhibition on NO production after hydroxylation at C-12 or C-15. Moreover, metabolite 2 dose-dependently reduced the LPS-induced protein expression of iNOS and COX-2., Competing Interests: Declaration of Competing Interest The authors have declared that there is no conflict of interest., (Copyright © 2020. Published by Elsevier Inc.)
- Published
- 2020
- Full Text
- View/download PDF
41. Triterpenoids with anti-proliferative effects from the seeds of Peganum harmala L.
- Author
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Li H, Wang Z, Wang Y, Xu J, and He X
- Subjects
- HeLa Cells, Humans, Plant Extracts, Seeds, Peganum, Triterpenes, Zygophyllaceae
- Abstract
Three undescribed lupane-type triterpenoids (1-3), three undescribed oleanane-type triterpenoids (4-6), and ten known pentacyclic triterpenoids (7-16) were isolated from the seeds of Peganum harmala L. (Zygophyllaceae). Their structures were elucidated using various spectroscopic methods (IR, HR-ESI-MS, 1D, and 2D NMR). All the triterpenoids were screened for anti-proliferative activity against HeLa, HepG2, and SGC-7901 cells using the MTT method. Except compounds 1, 2, and 13, all the other triterpenoids exhibited potent cytotoxic activities against tumour cells. Compounds 3, 6, and 15 inhibited the proliferation of HeLa cells in a dose-dependent manner, as observed by the colony formation assay. When HeLa cells were treated with different doses of compounds 3, 6, and 15, the cell nuclei changed shape to a crescent form and were condensed and fragmented, as observed by Hoechst 33258 staining. Additionally, these three triterpenoids induced the apoptosis in HeLa cells, which was detected by Western blot analysis., Competing Interests: Declaration of competing interest The authors have declared no conflict of interest., (Copyright © 2020 Elsevier Ltd. All rights reserved.)
- Published
- 2020
- Full Text
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42. Anti-neuroinflammatory triterpenoids from the seeds of Quercus serrata Thunb.
- Author
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Mai Y, Wang Z, Wang Y, Xu J, and He X
- Subjects
- Animals, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents pharmacology, Cell Line, Drug Evaluation, Preclinical, Mice, Microglia drug effects, Seeds chemistry, Triterpenes chemistry, Triterpenes pharmacology, Anti-Inflammatory Agents isolation & purification, Quercus chemistry, Triterpenes isolation & purification
- Abstract
The seeds of Quercus serrata Thunb. (acorns) have been served as a healthy food in Asia. In this study, five previously undescribed triterpenoids, along with nineteen known triterpenoids were isolated from acorns. Their structures were identified on account of spectroscopic analysis and GC-MS analysis after acid hydrolysis. Meanwhile, the inhibitory effects on nitric oxide (NO) production induced by lipopolysaccharide (LPS) in microglia cells (BV-2) of the identified triterpenoids were evaluated. All these compounds revealed overt anti-neuroinflammatory activity expect for 7. Compounds 1, 16 and 17 had potent inhibitory effects on protein and mRNA expression of iNOS and COX-2 in LPS-induced BV-2 cells. Bioactive triterpenoids 1, 2, 16 and 17 could block LPS-induced NF-κB nuclear translocation. This study suggested that acorns could be served as natural healthy product against neuroinflammatory-associated diseases, such as Alzheimer's disease., Competing Interests: Declaration of Competing Interest None, (Copyright © 2020 Elsevier B.V. All rights reserved.)
- Published
- 2020
- Full Text
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43. Anti-proliferative and anti-neuroinflammatory eudesmanolides from Wedelia (Sphagneticola trilobata (L.) Pruski).
- Author
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Sun L, Wang Z, Wang Y, Xu J, and He X
- Subjects
- Animals, Anti-Inflammatory Agents chemistry, Antineoplastic Agents, Phytogenic chemistry, Cell Line, Cyclooxygenase 2 genetics, Cyclooxygenase 2 metabolism, Dose-Response Relationship, Drug, Gene Expression Regulation drug effects, Humans, Lipopolysaccharides toxicity, Mice, Molecular Structure, Nitric Oxide Synthase Type II genetics, Nitric Oxide Synthase Type II metabolism, Anti-Inflammatory Agents pharmacology, Antineoplastic Agents, Phytogenic pharmacology, Sesquiterpenes chemistry, Sesquiterpenes pharmacology, Wedelia chemistry
- Abstract
Wedelia (Sphagneticola trilobata, Syn. Wedelia trilobata), a notoriously invasive agricultural weed, can inhibit the growth and development of crops in the field. Eight new eudesmanolides (1-8), along with twelve known congeners (9-20), were isolated and identified from the flowers of Wedelia. Their chemical structures were elucidated through various spectroscopic techniques. The isolates were tested for anti-proliferative activities against HeLa, HepG
2 , and SGC-7901 tumor cell lines. Most eudesmanolides exhibited significant anti-proliferative activities with good selectivity. Meanwhile, the cells morphology and clonogenic survival assays were observed for compounds 1-3. Moreover, compound 6 displayed obvious anti-neuroinflammatory effect by inhibition of NO production in LPS-induced microglia BV-2 cells with the IC50 value of 11.77 ± 0.83 μM. Hence, this study demonstrated that the eudesmanolides from Wedelia possessed significant antitumor and anti-inflammatory activity, which provided some guides for the further development and a new thinking for its control., Competing Interests: Declaration of competing interest There is not conflict of interest for this manuscript., (Copyright © 2019. Published by Elsevier B.V.)- Published
- 2020
- Full Text
- View/download PDF
44. Engineering of antibacterial/recyclable difunctional nanoparticles via synergism of quaternary ammonia salt site and N-halamine sites on magnetic surface.
- Author
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Chen Y, Feng C, Zhang Q, Luo M, Xu J, and Han Q
- Subjects
- Escherichia coli drug effects, Ferric Compounds chemistry, Microbial Sensitivity Tests, Nanoparticles ultrastructure, Photoelectron Spectroscopy, Silicon Dioxide chemistry, Spectroscopy, Fourier Transform Infrared, Staphylococcus aureus drug effects, Amines pharmacology, Anti-Bacterial Agents pharmacology, Magnetic Phenomena, Nanoparticles chemistry, Nanotechnology methods, Quaternary Ammonium Compounds pharmacology
- Abstract
A biocidal composite unit with improved synergism, using one cationic quaternary ammonia salt (QAS) site to attract electronegative bacteria to three highly biocidal N-halamine sites, was designed for the first time and attached onto surface of magnetic silica coated Fe
3 O4 nanoparticles (silica@Fe3 O4 NPs) for superior biocidability, large killing area, and easy recyclability. Briefly, a compound containing one imide and two amide NH bonds, 2-(2,5-dioxoimidazolidin-4-yl)-N-(4-hydroxyphenyl)acetamide (DHPA), was prepared by amidation of hydantoin acetic acid with p-aminophenol. A biocidal precursor of one QAS site and three N-halamine sites was then constructed by alcoholysis of 3-triethoxysilylpropyl succinic anhydride with 2-(dimethylamino)ethan-1-ol to introduce a tertiary amine and subsequent esterification with DHPA to introduce three NH bonds. The triethoxysilyl groups in the precursor were hydrolyzed to silanol groups to condense with their counterparts on silica@Fe3 O4 NPs. The surface of resultant NPs carried units each contains one QAS site and three N-halamine sites after quaternization and chlorination. The biocidal surface showed superior biocidability against Escherichia coli and Staphylococcus aureus than reported systems due to the improved synergism between multiple antibacterial groups of different types and was stable towards quenching-chlorinating process and storage. The successful design opens insight in the syntheses of more powerful biocides., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2020
- Full Text
- View/download PDF
45. Anti-neuroinflammatory benzofurans and lignans from Praxelis clematidea.
- Author
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Xiao L, Huang Y, Wang Y, Xu J, and He X
- Subjects
- Animals, Benzofurans isolation & purification, Cell Line, China, Lignans isolation & purification, Mice, Molecular Structure, Phytochemicals isolation & purification, Phytochemicals pharmacology, Asteraceae chemistry, Benzofurans pharmacology, Lignans pharmacology, Microglia drug effects
- Abstract
Praxelis clematidea (Asteraceae) is a harmful invasive alien weed, which cause huge destruction of ecological environment and economic losses. In this study, 22 compounds were isolated and purified from the whole plant of P. clematidea, including 4 benzofurans (1-4), 18 lignans (5-22), and five of which were new compounds (1, 3, 4, 9, 10). Various spectroscopic analysis methods were utilized to elucidate their chemical structures. The inhibitory effects of the isolated compounds on NO release from BV-2 microglia cells induced by LPS were investigated. Most of the compounds showed pronounced anti-neuroinflammatory activity. Additionally, the new compounds 3, 4 and 10 exhibited significant anti-neuroinflammatory effects, and the biological activities were further confirmed by immunoblotting, quantitative PCR and immunofluorescence staining assays. As results, this study provided a new idea for the further treatment and utilization of P. clematidea as anti-neuroinflammatory agents in health-benefit products., (Copyright © 2019. Published by Elsevier B.V.)
- Published
- 2020
- Full Text
- View/download PDF
46. The miR-15b-5p/PDK4 axis regulates osteosarcoma proliferation through modulation of the Warburg effect.
- Author
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Weng Y, Shen Y, He Y, Pan X, Xu J, Jiang Y, Zhang Q, Wang S, Kong F, Zhao S, Zhou D, and Zhang Y
- Subjects
- 3' Untranslated Regions, Animals, Apoptosis, Base Sequence, Binding Sites, Bone Neoplasms metabolism, Bone Neoplasms mortality, Bone Neoplasms pathology, Cell Line, Tumor, Cell Proliferation, Glycolysis genetics, Humans, Male, Mice, Mice, Nude, MicroRNAs metabolism, Osteoblasts pathology, Osteosarcoma metabolism, Osteosarcoma mortality, Osteosarcoma pathology, Protein Serine-Threonine Kinases antagonists & inhibitors, Protein Serine-Threonine Kinases metabolism, Pyruvate Dehydrogenase Acetyl-Transferring Kinase, RNA, Small Interfering genetics, RNA, Small Interfering metabolism, Signal Transduction, Survival Analysis, Xenograft Model Antitumor Assays, Bone Neoplasms genetics, Gene Expression Regulation, Neoplastic, MicroRNAs genetics, Osteoblasts metabolism, Osteosarcoma genetics, Protein Serine-Threonine Kinases genetics
- Abstract
Blocking aerobic glycolysis has been proposed as an attractive therapeutic strategy for impairing the proliferation of cancer cells. However, the underlying mechanisms are poorly understood. Here, we show that miR-15b-5p was downregulated in osteosarcoma (OS) and that lower expression of miR-15b-5p promoted proliferation and contributed to the Warburg effect in OS cells. Mechanistically, miR-15b-5p acted as a tumor suppressor in OS by directly targeting pyruvate dehydrogenase kinase-4 and inhibiting its expression. These results reveal a previously unknown function of miR-15b-5p in OS, which is associated with metabolic alterations that promote cancer progression. miR-15b-5p may play an essential role in the molecular therapy of patients with OS., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
- Full Text
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47. Drought monitoring based on TIGGE and distributed hydrological model in Huaihe River Basin, China.
- Author
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Zhao J, Xu J, Xie X, and Lu H
- Abstract
Drought assessment is important for developing measures to reduce agricultural vulnerability and thereby secure the livelihoods of those who depend on agriculture. This study uses four global ensemble weather prediction systems: the China Meteorological Administration (CMA), the European Centre for Medium-Range Weather Forecasts (ECMWF), the UK Met Office (UKMO), and the US National Centres for Environmental Prediction (NCEP) in the THORPEX (The Observing System Research and Predictability Experiment) Interactive Grand Global Ensemble (TIGGE) archive from 2006 to 2010. Based on results from the XXT (the first X denotes Xinanjiang, the second X denotes hybrid, and the T denotes TOPMODEL) distributed hydrological model, as well as soil moisture observations and digital elevation model (DEM) data, synthesized drought grades were established in the Huaihe River Basin of China. To filter out the impact of short-term fluctuations on observed soil moisture, a 30-day moving average was calculated. Use of the moving average significantly improves the correlation between observed soil moisture and simulated soil water deficit depth. Finally, a linear regression model describing the relationship between observed soil moisture and simulated soil water deficit depth was constructed. The deterministic regression coefficient was 0.5872, the correlation coefficient was 0.77, and the regression coefficient was -154.23. The trends in drought grades calculated using soil moisture and soil water deficit depth were found to be the same, and the grades agreed to within one level. Our findings highlight the importance of synthesizing drought grading when assessing drought using different soil moisture indicators in order to obtain a more comprehensive forecast of drought conditions., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
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48. Serum metabolomics research of the anti-hypertensive effects of Tengfu Jiangya tablet on spontaneously hypertensive rats.
- Author
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Jiang H, Shen Z, Chu Y, Li Y, Li J, Wang X, Yang W, Zhang X, Ju J, Xu J, and Yang C
- Subjects
- Animals, Antihypertensive Agents therapeutic use, Hypertension metabolism, Nitric Oxide biosynthesis, Rats, Rats, Inbred SHR, Rats, Inbred WKY, Antihypertensive Agents blood, Drugs, Chinese Herbal therapeutic use, Hypertension drug therapy, Metabolomics
- Abstract
A HPLC/TOF-MS-based metabolomic study was conducted to investigate the holistic therapeutic effects of Tengfu Jiangya Tablet (TJT) on spontaneously hypertensive rats (SHRs). The SHRs were divided into valsartan (VST) group, TJT group and model group, in addition, the Wistar-Kyoto rats (WKY) were taken as normal control. Serum samples were separated and identified by HPLC/TOF-MS, while the obtained data was further processed by partial least-squares discriminant analysis (PLS-DA). A clear cluster among the four groups was observed, and we identified thirteen biomarkers involved involved in sphingolipid metabolism (sphinganine, lysosphingomyelin, ceramide), glycerophospholipid metabolism (phosphatidylcholines, phosphatidylethanolamine, lysophosphatidylcholines), arginine and proline metabolism (l-proline, citrulline), tryptophan metabolism (xanthuiulrenic acid, l-kynurenine, l-tryptophan), arachidonic acid metabolism(leukotriene D4), and linoleic acid metabolism (gamma-linolenic acid). Altered metabolic pathways involved in impaired NO production, inflammation and vascular smooth muscle cells (VSMCs) apoptosis and proliferation, which suggesting the possible pathological state in SHRs. TJT as well as VST altered the metabolic state, suggesting a possible anti-hypertension role by improving NO production, and an extra cardiovascular protection role possibly by amelioration of inflammatory state and vascular remodeling., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
49. 3-(3-Hydroxy-4-methoxyphenyl)-4-(3,4,5-trimethoxyphenyl)-1,2,5-selenadiazole (G-1103), a novel combretastatin A-4 analog, induces G2/M arrest and apoptosis by disrupting tubulin polymerization in human cervical HeLa cells and fibrosarcoma HT-1080 cells.
- Author
-
Zuo D, Guo D, Jiang X, Guan Q, Qi H, Xu J, Li Z, Yang F, Zhang W, and Wu Y
- Subjects
- Bibenzyls chemistry, Caspase 8 metabolism, Caspase 9 metabolism, Cell Line, Tumor, Cyclin B1 metabolism, Down-Regulation drug effects, Female, Fibrosarcoma metabolism, Fibrosarcoma pathology, G2 Phase Cell Cycle Checkpoints drug effects, Guaiacol chemical synthesis, Guaiacol chemistry, Guaiacol toxicity, HeLa Cells, Humans, M Phase Cell Cycle Checkpoints drug effects, Microtubules metabolism, Organoselenium Compounds chemical synthesis, Organoselenium Compounds chemistry, Proto-Oncogene Proteins c-bcl-2 metabolism, Up-Regulation drug effects, Uterine Cervical Neoplasms metabolism, Uterine Cervical Neoplasms pathology, bcl-2-Associated X Protein metabolism, cdc25 Phosphatases metabolism, Apoptosis drug effects, Bibenzyls toxicity, Guaiacol analogs & derivatives, Organoselenium Compounds toxicity
- Abstract
Microtubule is a popular target for anticancer drugs. In this study, we describe the effect 3-(3-hydroxy-4-methoxyphenyl)-4-(3,4,5-trimethoxyphenyl)-1,2,5-selenadiazole (G-1103), a newly synthesized analog of combretastatin A-4 (CA-4), showing a strong time- and dose-dependent anti-proliferative effect on human cervical cancer HeLa cells and human fibrosarcoma HT-1080 cells. We demonstrated that the growth inhibitory effects of G-1103 in HeLa and HT-1080 cells were associated with microtubule depolymerization and proved that G-1103 acted as microtubule destabilizing agent. Furthermore, cell cycle analysis revealed that G-1103 treatment resulted in cell cycle arrest at the G2/M phase in a time-dependent manner with subsequent apoptosis induction. Western blot analysis revealed that down-regulation of cdc25c and up-regulation of cyclin B1 was related with G2/M arrest in HeLa and HT-1080 cells treatment with G-1103. In addition, G-1103 induced HeLa cell apoptosis by up-regulating cleaved caspase-3, Fas, cleaved caspase-8 expression, which indicated that G-1103 induced HeLa cell apoptosis was mainly associated with death receptor pathway. However, G-1103 induced HT-1080 cell apoptosis by up-regulating cleaved caspase-3, Fas, cleaved caspase-8, Bax and cleaved caspase-9 expression and down-regulating anti-apoptotic protein Bcl-2 expression, which indicated that G-1103 induced HT-1080 cell apoptosis was associated with both mitochondrial and death receptor pathway. Taken together, all the data demonstrated that G-1103 exhibited its antitumor activity through disrupting the microtubule assembly, causing cell cycle arrest and consequently inducing apoptosis in HeLa and HT-1080 cells. Therefore, the novel compound G-1103 is a promising microtubule inhibitor that has great potentials for therapeutic treatment of various malignancies., (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
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