24 results on '"Yang, Lifang"'
Search Results
2. Contributors
- Author
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Aroor, Annayya R., primary, Bartlett, Jordan J., additional, Belaid, Amine, additional, Benarroch-Popivker, Delphine, additional, Brest, Patrick, additional, Brown, Michael A., additional, Camera, Donny M., additional, Cao, Feng, additional, Ceylan, Asli F., additional, Chargui, Abderrahman, additional, Chen, Jing-Hai, additional, Crépeaux, Guillemette, additional, Cui, Taixing, additional, Dai, Dao-Fu, additional, Domdom, Marie Angela, additional, Filippakis, Harilaos, additional, Gherardi, Romain K., additional, Gillette, Thomas G., additional, Gilson, Eric, additional, Gracia-Sancho, Jordi, additional, Grosjean, Iris, additional, Guixé-Muntet, Sergi, additional, Han, Ying, additional, He, Ting, additional, Hofman, Paul, additional, Hu, Xin-Yang, additional, Jia, Guanghong, additional, Lagadic-Gossmann, Dominique, additional, Li, Linsen, additional, Lv, Jianjun, additional, Ma, Xi, additional, Ma, Sai, additional, Ma, Jipeng, additional, Maiese, Kenneth, additional, Maloyan, Alina, additional, Meyer, Mickael, additional, Miao, Chao-Yu, additional, Miyamoto, Shigeki, additional, Mograbi, Baharia, additional, Navratil, Amy M., additional, Perrotta, Ida, additional, Pulinilkunnil, Thomas, additional, Rabinovitch, Peter S., additional, Ren, Jun, additional, Rodrigues, Brian, additional, Roméo, Barnabé, additional, Sack, Michael N., additional, Smiles, William J., additional, Sowers, James R., additional, Sun, Dondong, additional, Sun, Yong, additional, Tan, Valerie P., additional, Tang, Yao-Liang, additional, Trivedi, Purvi C., additional, Wang, Xuejun, additional, Wang, Chen, additional, Wang, Jian’an, additional, Wang, Pei, additional, Wang, Shuyi, additional, Wang, Lu, additional, Wang, Xu-Dong, additional, Wang, Zhao V., additional, Xiao, Chang-Chen, additional, Yang, Zhi, additional, Yang, Mingjie, additional, Yang, Lifang, additional, Yang, Jian, additional, Yazbeck, Nathalie, additional, Yu, Hong, additional, Zhang, Yingmei, additional, Zhong, Qing, additional, and Zhu, Wei, additional
- Published
- 2018
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3. Small extracellular vesicles: Roles and clinical application in prostate cancer.
- Author
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Smack C, Johnson B, Nyalwidhe JO, Semmes OJ, and Yang L
- Subjects
- Humans, Male, Biomarkers, Tumor metabolism, Animals, Tumor Microenvironment, Prostatic Neoplasms pathology, Prostatic Neoplasms therapy, Extracellular Vesicles metabolism
- Abstract
Prostate cancer is a significant health problem in the United States. It is remarkably heterogenous, ranging from slow growing disease amenable to active surveillance to highly aggressive forms requiring active treatments. Therefore, being able to precisely determine the nature of disease and appropriately match patients to available and/or novel therapeutics is crucial to improve patients' overall outcome and quality of life. Recently small extracellular vesicles (sEVs), a subset of nanoscale membranous vesicles secreted by various cells, have emerged as important analytes for liquid biopsy and promising vehicles for drug delivery. sEVs contain various biomolecules such as genetic material, proteins, and lipids that recapitulate the characteristics and state of their donor cells. The application of existing and newly developed technologies has resulted in an increased depth of knowledge about biophysical structures, biogenesis, and functions of sEVs. In prostate cancer patients, tumor-derived sEVs can be isolated from biofluids, commonly urine and blood. They mediate intercellular signaling within the tumor microenvironment and distal organ-specific sites, supporting cancer initiation, progression, and metastasis. A mounting body of evidence suggests that sEV components can be potent biomarkers for prostate cancer diagnosis, prognosis, and prediction of disease progression and treatment response. Due to enhanced circulation stability and bio-barrier permeability, sEVs can be also used as effective drug delivery carriers to improve the efficacy and specificity of anti-tumor therapies. This review discusses recent studies on sEVs in prostate cancer and is focused on their role as biomarkers and drug delivery vehicles in the clinical management of prostate cancer., (Copyright © 2024. Published by Elsevier Inc.)
- Published
- 2024
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4. Advances of nanomedicine in treatment of atherosclerosis and thrombosis.
- Author
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Mao Y, Ren J, and Yang L
- Subjects
- Humans, Nanomedicine methods, Pharmaceutical Preparations, Nanoparticles, Atherosclerosis, Nanostructures, Thrombosis
- Abstract
Atherosclerosis (AS) is a chronic inflammatory vascular disease. Myocardial ischemia originated from AS is the main cause of cardiovascular diseases, one of the major factors contributing to the global disease burden. AS is typically quiescent until occurrence of plaque rupture and thrombosis, leading to acute coronary syndrome and sudden death. Currently, clinical diagnostic techniques suffer from major pitfalls including lack of accuracy and specificity, which makes it rather difficult for drugs to directly target plaques to achieve therapeutic effect. Therefore, how to accurately diagnose and effectively intervene vulnerable AS plaques to achieve accurate delivery of drugs has become an urgent and evolving clinical problem. With the rapid development of nanomedicine and nanomaterials, nanotechnology has shown unique advantages in monitoring vulnerable plaques and thrombus and improving drug efficacy. Recent studies have shown that application of nanoparticle drug delivery system can booster the safety and effectiveness of drug therapy, and molecular imaging technology and nanomedicine also exhibit high clinical application potentials in disease diagnosis. Therefore, nanotechnology provides another promising avenue for diagnosis and treatment of AS and thrombosis, and has shown excellent performance in the development of targeted drug therapy and biomaterials. In this review, the research progress, challenges and prospects of nanotechnology in AS and thrombosis are discussed, expecting to provide new ideas for the prevention, diagnosis and treatment of AS and thrombosis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023. Published by Elsevier Inc.)
- Published
- 2023
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5. Application of deep reinforcement learning for spike sorting under multi-class imbalance.
- Author
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Li S, Tang Z, Yang L, Li M, and Shang Z
- Subjects
- Action Potentials physiology, Microelectrodes, Algorithms, Signal Processing, Computer-Assisted, Neurons physiology
- Abstract
Spike sorting is the basis for analyzing spike firing patterns encoded in high-dimensional information spaces. With the fact that high-density microelectrode arrays record multiple neurons simultaneously, the data collected often suffers from two problems: a few overlapping spikes and different neuronal firing rates, which both belong to the multi-class imbalance problem. Since deep reinforcement learning (DRL) assign targeted attention to categories through reward functions, we propose ImbSorter to implement spike sorting under multi-class imbalance. We describe spike sorting as a Markov sequence decision and construct a dynamic reward function (DRF) to improve the sensitivity of the agent to minor classes based on the inter-class imbalance ratios. The agent is eventually guided by the optimal strategy to classify spikes. We consider the Wave_Clus dataset, which contains overlapping spikes and diverse noise levels, and the macaque dataset, which has a multi-scale imbalance. ImbSorter is compared with classical DRL architectures, traditional machine learning algorithms, and advanced overlapping spike sorting techniques on these two above datasets. ImbSorter obtained improved results on the Macro_F1. The results show ImbSorter has a promising ability to resist overlapping and noise interference. It has high stability and promising performance in processing spikes with different degrees of skewed distribution., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)
- Published
- 2023
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6. A long-wavelength mitochondria-targeted fluorescent probe for imaging of peroxynitrite during dexamethasone treatment.
- Author
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Tang J, Li Z, Qiang C, Han Y, Yang L, Zhu L, Dang T, Chen G, and Ye Y
- Subjects
- Mitochondria chemistry, Microscopy, Fluorescence methods, Optical Imaging, Dexamethasone analysis, Fluorescent Dyes chemistry, Peroxynitrous Acid analysis
- Abstract
Peroxynitrite (ONOO
- ), as a strong oxidizing reactive nitrogen substance (RNS), is generated endogenously by cells. Its visualization research is crucial to understand relevant disease processes. Herein, we reported a long-wavelength mitochondria-targeted fluorescence "turn on" probe TL. The probe TL could react with ONOO- by using 4-(Bromomethyl)benzeneboronic as a reactive site, which exhibited outstanding characteristics for detection of ONOO- , thus improving response time (about 50 s), sensitivity (DL, 10.1 nM), and emission wavelength (667 nm). Besides, TL displayed well mitochondria targeting and biological visualizing of exogenous and endogenous ONOO- in biological systems. Finally, TL was used to monitor high concentration of dexamethasone-induced an up-regulation of ONOO- . This indicated that TL has excellent potential to study the fluctuation of ONOO- in the physiological and pathological system., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)- Published
- 2023
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7. Corrigendum to "Deficiency in adiponectin exaggerates cigarette smoking exposure-induced cardiac contractile dysfunction: Role of autophagy" [Pharm. Res. 100 (2015) 175-89].
- Author
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Hu N, Yang L, Dong M, Ren J, and Zhang Y
- Published
- 2022
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8. Oncogenic viral infection and amino acid metabolism in cancer progression: Molecular insights and clinical implications.
- Author
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Liu N, Shi F, Yang L, Liao W, and Cao Y
- Subjects
- Amino Acids, Glutamine metabolism, Humans, Neoplasms pathology, Oncogenic Viruses
- Abstract
Viruses lack essential living system, so they must hijack host cell metabolism for its survival and reproduction. Interestingly, the metabolic reprogramming induced by oncovirus is critical for the malignant transformation. Amino acid can supply the source of nitrogen and carbon for biosynthesis or fulfill the energy requirement for the rapid growth of tumor cells. Amino acid metabolism caused by oncogenic viral infection often mirrors metabolic changes observed in cancer cells, such as glutamine addiction, asparagine dependence, arginine auxotrophy and active serine/ proline metabolism. In this review, we describe amino acid metabolism reprogramming in tumors. We also discuss how oncogenic viruses hijack amino acid metabolism in the stress status. Further research on the metabolic profile of virus-related cancers will not only provide new targets for tumor prevention and treatment, but novel diagnostic and therapeutic strategies as well., (Copyright © 2022 Elsevier B.V. All rights reserved.)
- Published
- 2022
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9. Design, synthesis and biological evaluation of 2,3-dihydro-[1,4]dioxino[2,3-f]quinazoline derivatives as EGFR inhibitors.
- Author
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Qin X, Yang L, Liu P, Yang L, Chen L, Hu L, and Jiang M
- Subjects
- Cell Line, Tumor, Cell Survival drug effects, Drug Design, Humans, Molecular Docking Simulation, Antineoplastic Agents chemical synthesis, Antineoplastic Agents pharmacology, Quinazolines chemical synthesis, Quinazolines pharmacology
- Abstract
Epidermal growth factor receptor (EGFR) is the most attractive target for drug research in non-small cell lung cancer (NSCLC). The first-generation EGFR tyrosine kinase inhibitors (TKIs) Gefetinib and Elotinib showed good clinical efficacy on lung adenocarcinoma tumors, but almost all patients developed resistance to these inhibitors over time. Quinazoline and quinoline derivatives are common targeted inhibitors of EGFR kinase, and their structural optimization is an important direction for the development of effective targeted anticancer drugs. Based on these facts, a series of heterocyclic 2,3-dihydro-[1,4]dioxino[2,3-f]quinazoline derivatives have been designed and synthesized and their structures were confirmed by spectral analyses. The cytotoxic activity of the newly synthesized compounds was evaluated against the human kidney epithelial T293 cell line, normal lung cell lines WI-38, non-small cell lung cancer A549 and NCI-H157 cell lines using MTT. The tested compounds showed an evident anticancer activity against the tested cell lines, especially compound 13c, which was the most potent anticancer agent with half maximal inhibitory concentrations (IC
50 ) between 8.82 and 10.24 μM. Docking study showed that compound 13b could be nicely bound to the ATP binding pocket of EGFR. In addition, the inhibitory activity of the target compounds against epidermal growth factor receptor tyrosine kinase (EGFR-TK) was evaluated. Results indicated the ability of the target compounds to inhibit EGFR-TK with half maximal inhibitory concentrations (IC50 ) in the range of 10.29 nM to 652.3 nM. In view of the reported compound activity, the structure deserves further optimization as cancer treatment agents., (Copyright © 2021. Published by Elsevier Inc.)- Published
- 2021
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10. Hydrothermal synthesis of fluorescent carbon dots from gardenia fruit for sensitive on-off-on detection of Hg 2+ and cysteine.
- Author
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Sun D, Liu T, Wang C, Yang L, Yang S, and Zhuo K
- Subjects
- Carbon, Cysteine, Fluorescent Dyes, Fruit, Nitrogen, Spectrometry, Fluorescence, Gardenia, Mercury, Quantum Dots
- Abstract
Nitrogen and sulfur co-doped carbon dots (N/S-CDs) were prepared by a simple hydrothermal method using gardenia fruit as precursor. The N/S-CDs are nearly spherical particles with a size of 2.1 nm and possess excellent fluorescence stability in a wide pH range and high NaCl concentrations, as well as under UV light irradiation. The absolute quantum yield (QY) without any surface modification reaches up to 10.7%. Meanwhile, the N/S-CDs can be quenched by Hg
2+ (turn-off), while the quenched fluorescence can be recovered (turn-on) by introducing cysteine (Cys), with linear ranges of 2-20 μM for Hg2+ and 0.1-2.0 μM for Cys. On the basis of the on-off-on sensing mode, the N/S-CDs can be used to detect Hg2+ and Cys. Hence, the N/S-CDs would be a promising sensor in environmental and biological analysis., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)- Published
- 2020
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11. Automatic bad channel detection in implantable brain-computer interfaces using multimodal features based on local field potentials and spike signals.
- Author
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Li M, Liang Y, Yang L, Wang H, Yang Z, Zhao K, Shang Z, and Wan H
- Subjects
- Action Potentials, Machine Learning, Brain-Computer Interfaces
- Abstract
"Bad channels" in implantable multi-channel recordings bring troubles into the precise quantitative description and analysis of neural signals, especially in the current "big data" era. In this paper, we combine multimodal features based on local field potentials (LFPs) and spike signals to detect bad channels automatically using machine learning. On the basis of 2632 pairs of LFPs and spike recordings acquired from five pigeons, 12 multimodal features are used to quantify each channel's temporal, frequency, phase and firing-rate properties. We implement seven classifiers in the detection tasks, in which the synthetic minority oversampling technique (SMOTE) system and Fisher weighted Euclidean distance sorting (FWEDS) are used to cope with the class imbalance problem. The results of the two-dimensional scatterplots and classifications demonstrate that correlation coefficient, phase locking value, and coherence have good discriminability. For the multimodal features, almost all the classifiers can obtain high accuracy and bad channel detection rate after the SMOTE operation, in which the Random Forests classifier shows relatively better comprehensive performance (accuracy: 0.9092 ± 0.0081, precision: 0.9123 ± 0.0100, and recall: 0.9057 ± 0.0121). The proposed approach can automatically detect bad channels based on multimodal features, and the results provide valuable references for larger datasets., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2019 Elsevier Ltd. All rights reserved.)
- Published
- 2020
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12. CD74 knockout attenuates alcohol intake-induced cardiac dysfunction through AMPK-Skp2-mediated regulation of autophagy.
- Author
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Yang L, Wang S, Ma J, Li J, Yang J, Bucala R, and Ren J
- Subjects
- Animals, Antigens, Differentiation, B-Lymphocyte metabolism, Calcium metabolism, Histocompatibility Antigens Class II metabolism, Intramolecular Oxidoreductases genetics, Intramolecular Oxidoreductases metabolism, Macrophage Migration-Inhibitory Factors genetics, Macrophage Migration-Inhibitory Factors metabolism, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Mitochondria metabolism, Muscle Contraction drug effects, Myocardium pathology, Myocytes, Cardiac cytology, Myocytes, Cardiac drug effects, Myocytes, Cardiac metabolism, RNA Interference, RNA, Small Interfering metabolism, S-Phase Kinase-Associated Proteins antagonists & inhibitors, S-Phase Kinase-Associated Proteins genetics, Sirtuin 1 chemistry, Sirtuin 1 metabolism, TOR Serine-Threonine Kinases metabolism, AMP-Activated Protein Kinases metabolism, Antigens, Differentiation, B-Lymphocyte genetics, Autophagy drug effects, Ethanol pharmacology, Histocompatibility Antigens Class II genetics, Myocardium metabolism, S-Phase Kinase-Associated Proteins metabolism
- Abstract
CD74, a non-polymorphic type II transmembrane glycoprotein and MHC class II chaperone, is the cell surface receptor for the inflammatory cytokine macrophage migration inhibitory factor (MIF) and participates in inflammatory signaling regulation. This study examined the potential role of CD74 in binge drinking-induced cardiac contractile dysfunction. WT and CD74 knockout mice were exposed to ethanol (3 g/kg/d, i.p., for 3 days). Echocardiography, cardiomyocyte function, histological staining and autophagy signaling including AMPK, mTOR, and AMPK downstream signals Skp2 and Sirt1 were evaluated. Our results revealed that ethanol challenge overtly compromised echocardiographic, cardiomyocyte contractile, intracellular Ca
2+ and ultrastructural properties along with overt apoptosis, inflammation (elevated MIF, IL-1β and IL-6) and mitochondrial O2 - production (p < 0.01), the effect of which was reconciled by CD74 ablation (p < 0.01 vs. ethanol group) with the exception of MIF expression. Ethanol challenge upregulated autophagy (p < 0.001), promoted AMPK phosphorylation and Sirt1 levels (p < 0.003) while suppressing mTOR phosphorylation and Skp2 levels (p < 0.02). These effects were reversed by CD74 ablation. In vitro studies demonstrated that short-term ethanol challenge compromised cardiomyocyte contractile function and facilitated GFP-Puncta formation, which were mitigated by CD74 knockout (p < 0.0001). Moreover, the CD74 ablation-offered beneficial effects against ethanol-induced cardiomyocyte dysfunction, and GFP-Puncta formation were nullified by the AMPK activator AICAR, the Skp2 inhibitor C1 or the Sirt1 activator SRT1720 (p < 0.0001). Taken together, our data revealed that CD74 ablation counteracts acute ethanol challenge-induced myocardial dysfunction, inflammation and apoptosis possibly through an AMPK-mTOR-Skp2-mediated regulation of autophagy., (Copyright © 2019 Elsevier B.V. All rights reserved.)- Published
- 2019
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13. Transcriptome analyses of Paris polyphylla var. chinensis, Ypsilandra thibetica, and Polygonatum kingianum characterize their steroidal saponin biosynthesis pathway.
- Author
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Yang Z, Yang L, Liu C, Qin X, Liu H, Chen J, and Ji Y
- Subjects
- Biosynthetic Pathways, Cytochrome P-450 Enzyme System genetics, Gene Expression Profiling, Liliaceae chemistry, Liliaceae metabolism, Melanthiaceae chemistry, Melanthiaceae metabolism, Molecular Structure, Phylogeny, Phytosterols chemistry, Phytosterols genetics, Polygonatum chemistry, Polygonatum metabolism, Saponins chemistry, Saponins genetics, Triterpenes, Liliaceae genetics, Melanthiaceae genetics, Phytosterols biosynthesis, Polygonatum genetics, Saponins biosynthesis, Transcriptome
- Abstract
Steroidal saponins, one of the most diverse groups of plant-derived natural products, elicit biological and pharmacological activities; however, the genes involved in their biosynthesis and the corresponding biosynthetic pathway in monocotyledon plants remain unclear. This study aimed to identify genes involved in the biosynthesis of steroidal saponins by performing a comparative analysis among transcriptomes of Paris polyphylla var. chinensis (PPC), Ypsilandra thibetica (YT), and Polygonatum kingianum (PK). De novo transcriptome assemblies generated 57,537, 140,420, and 151,773 unigenes from PPC, YT, and PK, respectively, of which 56.54, 47.81, and 44.30% were successfully annotated, respectively. Among the transcriptomes for PPC, YT, and PK, we identified 194, 169, and 131; 17, 14, and 26; and, 80, 122, and 113 unigenes corresponding to terpenoid backbone biosynthesis; sesquiterpenoid and triterpenoid biosynthesis; and, steroid biosynthesis pathways, respectively. These genes are putatively involved in the biosynthesis of cholesterol that is the primary precursor of steroidal saponins. Phylogenetic analyses indicated that lanosterol synthase may be exclusive to dicotyledon plant species, and the cytochrome P450 unigenes were closely related to clusters CYP90B1 and CYP734A1, which are UDP-glycosyltransferases unigenes homologous with the UGT73 family. Thus, unigenes of β-glucosidase may be candidate genes for catalysis of later period modifications of the steroidal saponin skeleton. Our data provide evidence to support the hypothesis that monocotyledons biosynthesize steroidal saponins from cholesterol via the cycloartenol pathway., (Copyright © 2019. Published by Elsevier B.V.)
- Published
- 2019
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14. Rutin attenuates doxorubicin-induced cardiotoxicity via regulating autophagy and apoptosis.
- Author
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Ma Y, Yang L, Ma J, Lu L, Wang X, Ren J, and Yang J
- Subjects
- Animals, Cardiotoxins pharmacology, Doxorubicin pharmacology, Male, Mice, Apoptosis drug effects, Autophagy drug effects, Cardiotoxins adverse effects, Doxorubicin adverse effects, Rutin pharmacology
- Abstract
Doxorubicin as anticancer agent can cause dose-dependent cardiotoxicity and heart failure in the long term. Rutin as a polyphenolic flavonoid has been illustrated to protect hearts from diverse cardiovascular diseases. Its function is known to be related to its antioxidant and antiinflammatory activity which may regulate multiple cellular signal pathways. However, the role of rutin on doxorubicin-induced cardiotoxicity has yet to be discovered. In this study, we explored the protective role of rutin on doxorubicin-induced heart failure and elucidated the potential mechanisms of protective effects of rutin against cardiomyocyte death. We analyzed cardiac tissues at the time point of 8weeks after doxorubicin treatment. The results by echocardiography, TUNEL staining, Masson's trichrome staining as well as Western blot analysis revealed that doxorubicin induced remarkable cardiac dysfunction and cardiotoxicity in mice hearts and cardiomyocytes, which were alleviated by rutin treatment. Western blot analysis indicated that the underlying mechanisms included inhibition excessive autophagy and apoptosis mediated by Akt activation. Collectively, our findings suggest that suppression of autophagy and apoptosis by administration of rutin could attenuate doxorubicin-induced cardiotoxicity, which enhances our knowledge to explore new drugs and strategies for combating this devastating side effect induced by doxorubicin. This article is part of a Special Issue entitled: Genetic and epigenetic control of heart failure - edited by Jun Ren & Megan Yingmei Zhang., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
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15. Deficiency in adiponectin exaggerates cigarette smoking exposure-induced cardiac contractile dysfunction: Role of autophagy.
- Author
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Hu N, Yang L, Dong M, Ren J, and Zhang Y
- Subjects
- Animals, Apoptosis physiology, Calcium metabolism, Cardiomyopathies metabolism, Lysosomes metabolism, Mice, Mice, Inbred C57BL, Mice, Knockout, Myocardium metabolism, Myocytes, Cardiac metabolism, Reactive Oxygen Species metabolism, Adiponectin deficiency, Autophagy physiology, Myocardial Contraction physiology, Smoking adverse effects
- Abstract
Second hand smoke is an independent risk factor for cardiovascular disease. Adiponectin (APN), an adipose-derived adipokine, has been shown to offer cardioprotective effect through an AMPK-dependent manner. This study was designed to evaluate the impact of adiponectin deficiency on second hand smoke-induced cardiac pathology and underlying mechanisms using a mouse model of side-stream smoke exposure. Adult wild-type (WT) and adiponectin knockout (APNKO) mice were placed in a chamber exposed to cigarette smoke for 1 hour daily for 40 days. Echocardiographic, cardiomyocyte function, and intracellular Ca2+ handling were evaluated. Autophagy and apoptosis were examined using western blot. 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) staining was used to evaluate reactive oxygen species (ROS) generation. Masson trichrome staining was employed to measure interstitial fibrosis. Our data revealed that adiponectin deficiency provoked smoke exposure-induced cardiomyopathy (compromised fractional shortening, disrupted cardiomyocyte function and intracellular Ca2+ homeostasis, apoptosis and ROS generation). In addition, these detrimental effects of side-stream smoke were accompanied by defective autophagolysosome formation, the effect of which was exacerbated by adiponectin deficiency. Blocking autophagolysosome formation using bafilomycin A1 (BafA1) negated the cardioprotective effect of rapamycin against smoke extract. Induction of autophagy using rapamycin and AMPKα activation using AICAR rescued against smoke extract-induced myopathic anomalies in APNKO mice. Our data suggest that adiponectin serves as an indispensable cardioprotective factor against side-stream smoke exposure-induced myopathic changes possibly through facilitating autophagolysosome formation., (Copyright © 2015 Elsevier Ltd. All rights reserved.)
- Published
- 2015
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16. Cardiac-specific overexpression of metallothionein attenuates myocardial remodeling and contractile dysfunction in l-NAME-induced experimental hypertension: Role of autophagy regulation.
- Author
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Yang L, Gao JY, Ma J, Xu X, Wang Q, Xiong L, Yang J, and Ren J
- Subjects
- Animals, Calcium metabolism, Hypertension chemically induced, Male, Mice, Oxidative Stress, Reactive Oxygen Species metabolism, Sirolimus pharmacology, Autophagy physiology, Hypertension physiopathology, Metallothionein physiology, Myocardial Contraction, Myocardium pathology, NG-Nitroarginine Methyl Ester toxicity
- Abstract
Hypertension is an independent risk factor for heart disease and is responsible for the increased cardiac morbidity and mortality. Oxidative stress plays a key role in hypertensive heart diseases although the precise mechanism remains unclear. This study was designed to examine the effect of cardiac-specific overexpression of metallothionein, a cysteine-rich antioxidant, on myocardial contractile and intracellular Ca(2+) anomalies in N(G)-nitro-l-arginine methyl ester (l-NAME)-induced experimental hypertension and the mechanism involved with a focus on autophagy. Our results revealed that l-NAME treatment (14 days) led to hypertension and myocardial anomalies evidenced by interstitial fibrosis, cardiomyocyte hypertrophy, increased LV end systolic and diastolic diameters (LVESD and LVEDD) along with suppressed fractional shortening. l-NAME compromised cardiomyocyte contractile and intracellular Ca(2+) properties manifested as depressed peak shortening, maximal velocity of shortening/relengthening, electrically-stimulated rise in intracellular Ca(2+), elevated baseline and peak intracellular Ca(2+). These l-NAME-induced histological and mechanical changes were attenuated or reconciled by metallothionein. Protein levels of autophagy markers LC3B and p62 were decreased and increased, respectively. Autophagy signaling molecules AMPK, TSC2 and ULK1 were inactivated while those of mTOR and p70s6K were activated by l-NAME, the effects of which were ablated by metallothionein. Autophagy induction mimicked whereas autophagy inhibition nullified the beneficial effect of metallothionein against l-NAME. These findings suggested that metallothionein protects against l-NAME-induced myocardial anomalies possibly through restoration of autophagy., (Copyright © 2015 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2015
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17. Heavy metal scavenger metallothionein attenuates ER stress-induced myocardial contractile anomalies: role of autophagy.
- Author
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Yang L, Hu N, Jiang S, Zou Y, Yang J, Xiong L, and Ren J
- Subjects
- Acetylcysteine pharmacology, Adenine analogs & derivatives, Adenine pharmacology, Animals, Autophagy physiology, Echocardiography, Male, Mice, Mice, Transgenic, Myocardium cytology, Myocytes, Cardiac cytology, Myocytes, Cardiac metabolism, Tunicamycin administration & dosage, Endoplasmic Reticulum Stress physiology, Heart physiology, Metallothionein metabolism, Myocardial Contraction physiology, Myocardium metabolism
- Abstract
Endoplasmic reticulum (ER) stress increases the risk of cardiovascular morbidity and mortality although the underlying mechanism remains elusive. This study was designed to examine the impact of cardiac over-expression of metallothionein, a cysteine-rich heavy metal scavenger, on ER stress-induced changes in myocardial function and underlying mechanism involved with a focus on autophagy. Wild-type friendly virus B (FVB) and metallothionein transgenic mice were subjected to the ER stress inducer tunicamycin (1 mg/kg). Our results showed that ER stress led to compromised echocardiographic and cardiomyocyte contractile function, intracellular Ca(2+) mishandling. Tunicamycin promoted ER stress and oxidative stress, increased left ventricular end systolic and diastolic diameter, as well as suppressed fractional shortening and whole heart contractility, the effects of which were significantly attenuated or ablated by metallothionein. Levels of the autophagy markers such as phosphorylated ULK1, Atg5, Atg7, LC3B and the autophagy adaptor p62 were significantly upregulated. These ER stress-induced changes in myocardial function, autophagy and autophagy signaling were distinctly mitigated or alleviated by metallothionein. Inhibition of autophagy using 3-methyladenine in vitro reversed ER stress-induced cardiomyocyte contractile defects. Meanwhile, ER stress-induced cardiomyocyte dysfunction was attenuated by the antioxidant N-acetylcysteine. Collectively, these findings suggested that metallothionein protects against ER stress-induced cardiac anomalies possibly through attenuation of cardiac autophagy., (Copyright © 2014 Elsevier Ireland Ltd. All rights reserved.)
- Published
- 2014
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18. Transcatheter closure of congenital perimembranous ventricular septal defect in children using symmetric occluders: an 8-year multiinstitutional experience.
- Author
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Wang L, Cao S, Li J, Yang L, Liu Y, Ren J, Ma Q, Xing H, Li D, Tian D, Wan Y, Yu S, Chen T, Yang X, and Yang J
- Subjects
- Cardiac Catheterization, Cardiac Surgical Procedures methods, Child, Child, Preschool, Cohort Studies, Female, Follow-Up Studies, Humans, Male, Prosthesis Design, Time Factors, Heart Septal Defects, Ventricular surgery, Septal Occluder Device
- Abstract
Background: Perimembranous ventricular septal defects (pmVSDs) are one of the most common forms of congenital cardiac malformation in children. Results of transcatheter pmVSD closure remain debatable, prompting the need for further evaluation with regard to the safety and efficacy of this procedure. The aim of the study was to analyze the safety, efficacy, and long-term follow-up data associated with transcatheter closure of pmVSDs in children using symmetric occluders., Methods: From December 2002 to October 2011, 525 children with pmVSDs between 2 and 12 years of age underwent transcatheter closure at three major heart centers in northwest China with symmetric pmVSD occluders. All patients were followed up until October 2011 with electrocardiogram and transthoracic echocardiography. Adverse events were recorded and evaluated., Results: There were 252 male and 273 female patients with an average weight of 21.5 kg. The mean age at the time of transcatheter closure was 5.6 years, and the average ratio of pulmonic to systemic blood flow was 2.5. Transcatheter intervention was successfully performed in 502 patients (95.6%). The median device size implanted was 6.5 mm (range, 4 to 18 mm). During a median 45-month follow-up period, no mortality occurred. A total of three major adverse events (0.6%) were reported; two were valve-related. Meanwhile, 104 minor adverse events were detected during the entire follow-up period. All individuals experiencing major adverse events were younger than 3 years of age. The incidence of major adverse events in patients younger than 3 years old was significantly higher than that of patients older than 3 years old (3.75% versus 0.00%; Fisher's exact test p=0.004)., Conclusions: Data from the current study suggest that transcatheter pmVSD closure using symmetric occluders displayed an excellent success rate and long-term follow-up results. The transcatheter approach provides a less-invasive alternative to open surgery and displays some promise in the treatment of pmVSDs in certain patient populations., (Copyright © 2012 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.)
- Published
- 2012
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19. EBV encoded miR-BHRF1-1 potentiates viral lytic replication by downregulating host p53 in nasopharyngeal carcinoma.
- Author
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Li Z, Chen X, Li L, Liu S, Yang L, Ma X, Tang M, Bode AM, Dong Z, Sun L, and Cao Y
- Subjects
- Carcinoma, Cell Line, Down-Regulation, Herpesvirus 4, Human metabolism, Humans, Nasopharyngeal Carcinoma, Nasopharyngeal Neoplasms genetics, Nasopharyngeal Neoplasms metabolism, Tumor Suppressor Protein p53 metabolism, Viral Proteins metabolism, Virus Replication, Herpesvirus 4, Human genetics, MicroRNAs metabolism, Nasopharyngeal Neoplasms virology, Tumor Suppressor Protein p53 genetics
- Abstract
miRNAs (microRNAs) are a class of non-coding small RNAs. The Epstein-Barr-virus (EBV) encoded miR-BHRF1-1 is barely expressed in most nasopharyngeal carcinoma (NPC) cells with EBV latent infection. Here, we used a strategy of overexpression and inhibition of miR-BHRF1-1 and showed that miR-BHRF1-1 is involved in TPA-induced accumulation of EBV lytic proteins and viral copies in late lytic cycle. The data further suggested that the miR-BHRF1-1-potentiated induction of EBV lytic replication was accompanied by inhibiting p53 expression. Our results demonstrated that the EBV original pathogen miR-BHRF1-1 is involved in the control of EBV late lytic replication by directly targeting the host p53 gene., (Copyright © 2011 Elsevier Ltd. All rights reserved.)
- Published
- 2012
- Full Text
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20. ZL11n is a novel nitric oxide-releasing derivative of farnesylthiosalicylic acid that induces apoptosis in human hepatoma HepG2 cells via MAPK/mitochondrial pathways.
- Author
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Yang L, Ling Y, Zhang Z, Zhao Q, Tang J, Ji H, and Zhang Y
- Subjects
- Animals, Antineoplastic Agents chemistry, Antineoplastic Agents therapeutic use, Carcinoma, Hepatocellular enzymology, Carcinoma, Hepatocellular pathology, Enzyme Activation, Farnesol chemistry, Farnesol pharmacokinetics, Farnesol therapeutic use, Hep G2 Cells, Humans, Liver Neoplasms enzymology, Liver Neoplasms pathology, Male, Mice, Mice, Inbred ICR, Mitochondria enzymology, Nitric Oxide metabolism, Oxadiazoles chemistry, Oxadiazoles therapeutic use, Salicylates chemistry, Antineoplastic Agents pharmacology, Apoptosis, Carcinoma, Hepatocellular drug therapy, Farnesol analogs & derivatives, Liver Neoplasms drug therapy, Mitochondria drug effects, Mitogen-Activated Protein Kinase Kinases metabolism, Oxadiazoles pharmacokinetics
- Abstract
ZL11n is a novel furoxan-based nitric oxide (NO)-releasing derivative of farnesylthiosalicylic acid. In this study, we examined the anticancer effects and the potential mechanism of action of ZL11n in vitro and in vivo. It was found that ZL11n exhibited a favorable, selective cytotoxic effect in the HepG2 cell line. The yield of NO in the ZL11n treated HepG2 cells was much higher than in the control group and the normal human liver L-02 cells. Furthermore, the NO concentration was correlated to the degree of cytotoxicity observed. The ZL11n-induced apoptosis was assessed by Annexin V-FITC/propidium iodide flow cytometry assay. ZL11n triggered the mitochondrial/caspase apoptotic pathway by decreasing mitochondrial membrane potential, cytochrome c release from mitochondrial, and reducing the Bcl-2-to-Bax ratio, in addition to activating the caspase cascade. Simultaneously, we found that ZL11n treatment led to an increase in JNK and ERK1/2 phosphorylation. Furthermore, treatment with SP600125 (a JNK inhibitor) and PD98059 (an ERK1/2 inhibitor) prior to ZL11n treatment was found to significantly reverse ZL11n-induced apoptosis. The in vivo findings also revealed that ZL11n significantly reduced tumor volume and weight in the H(22) solid tumor mouse model examined. In short, our findings suggest that ZL11n induced apoptosis through the coordination of the mitochondrial apoptotic pathway (activated by NO) and MAPKs signaling pathway (triggered by JNK or ERK)., (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Published
- 2011
- Full Text
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21. Cardioprotective effects of electroacupuncture pretreatment on patients undergoing heart valve replacement surgery: a randomized controlled trial.
- Author
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Yang L, Yang J, Wang Q, Chen M, Lu Z, Chen S, and Xiong L
- Subjects
- Cardiotonic Agents therapeutic use, Female, Humans, Intensive Care Units, Length of Stay, Male, Middle Aged, Myocardial Reperfusion Injury etiology, Single-Blind Method, Troponin I blood, Cardiopulmonary Bypass adverse effects, Electroacupuncture, Heart Valve Prosthesis Implantation adverse effects, Ischemic Preconditioning, Myocardial, Myocardial Reperfusion Injury prevention & control, Preoperative Care
- Abstract
Background: Cardiac ischemia-reperfusion injury after cardiopulmonary bypass contributes to postoperative morbidity and mortality in patients with open-heart surgery. This randomized controlled trial was designed to address the protective effects of electroacupuncture (EA) pretreatment on myocardial injury in patients undergoing heart valve replacement surgery., Methods: Sixty patients with acquired heart valve disease were randomly allocated to the EA pretreatment group or the control group. Patients in the EA group received EA stimulus at bilateral Neiguan (PC 6), Lieque (LU 7), and Yunmen (LU 2) for 30 minutes each day for five consecutive days before surgery. Hemodynamic data, mechanical ventilation time, inotropic drug use in the intensive care unit, serum cardiac troponin I concentrations, morbidities, and mortalities were compared between the two groups. This trial is registered with ClinicalTrials.gov, number NCT00732459., Results: At 6 hours, 12 hours, and 24 hours after reperfusion, levels of serum cardiac troponin I were significantly decreased in the EA group (5.74 +/- 0.67, 6.22 +/- 0.66, and 5.21 +/- 0.58) compared with that in the control group (7.89 +/- 0.74, 8.34 +/- 1.08, and 7.57 +/- 0.89, p < 0.05). The EA pretreatment significantly reduced overall serum troponin I release at 6 hours, 12 hours, and 24 hours after aortic cross-clamp removal. Meanwhile, EA pretreatment also reduced the inotrope score at 12 hours, 24 hours, and 48 hours after the intensive care unit arrival and shortened intensive care unit stay time (p < 0.05)., Conclusions: The present study demonstrated that EA pretreatment may alleviate cardiac ischemia-reperfusion injury in adult patients undergoing heart valve replacements. This simple and convenient treatment has the potential to be used in the clinic for reducing myocardial injury in patients with heart valve replacement surgery., (2010 The Society of Thoracic Surgeons. Published by Elsevier Inc. All rights reserved.)
- Published
- 2010
- Full Text
- View/download PDF
22. Transcatheter closure of perimembranous ventricular septal defect in a patient with abnormal inferior vena cava return.
- Author
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Wang L, Yang L, Ma Q, Xing H, and Yang J
- Subjects
- Child, Heart Septal Defects, Ventricular diagnostic imaging, Heart Septal Defects, Ventricular therapy, Humans, Male, Radiography, Vena Cava, Inferior diagnostic imaging, Cardiac Catheterization methods, Heart Septal Defects, Ventricular diagnosis, Vena Cava, Inferior abnormalities
- Abstract
An 8-year-old boy with a 3.8 mm perimembranous ventricular septal defect (PmVSD) and abnormal inferior vena cava return was attempted cardiac catheterization to occlude the VSD. Through the right jugular vein and right femoral artery approach, an 8 mm Amplatzer VSD occluder was successfully deployed under fluoroscopic and echocardiographic guidance. After a 2 year follow-up, the patient is symptom-free. Our report offers a new perspective to percutaneous treatment of PmVSD with abnormal inferior vena cava return.
- Published
- 2009
- Full Text
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23. Exceptional survival: acute coronary syndrome in a 56-year-old patient with Takayasu's arteritis.
- Author
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Yang J, Yang L, Liu J, Zhang J, Zhao H, and Yi D
- Subjects
- Acute Coronary Syndrome diagnostic imaging, Aortography, Coronary Angiography, Female, Humans, Middle Aged, Recovery of Function, Survival, Takayasu Arteritis diagnostic imaging, Acute Coronary Syndrome complications, Acute Coronary Syndrome physiopathology, Takayasu Arteritis complications, Takayasu Arteritis physiopathology
- Published
- 2009
- Full Text
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24. Patent carotid arteries with nearly occluded aortic arch in Takayasu's arteritis demonstrated by multidetector-row computed tomography.
- Author
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Yang J, Yang L, Zhang J, Zheng M, Zhao H, and Yi D
- Subjects
- Adult, Aorta, Thoracic diagnostic imaging, Arterial Occlusive Diseases etiology, Carotid Artery, Common diagnostic imaging, Contrast Media, Female, Humans, Subclavian Artery diagnostic imaging, Vascular Patency, Arterial Occlusive Diseases diagnostic imaging, Takayasu Arteritis complications, Tomography, X-Ray Computed methods
- Published
- 2007
- Full Text
- View/download PDF
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