1. Bioremediase a unique protein from a novel bacterium BKH1, ushering a new hope in concrete technology
- Author
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Biswas, Mousumi, Majumdar, Sudipta, Chowdhury, Trinath, Chattopadhyay, Brajadulal, Mandal, Saroj, Halder, Umesh, and Yamasaki, Shinji
- Subjects
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BACTERIAL proteins , *NUCLEOTIDE sequence , *CONCRETE , *PHYLOGENY , *CARBONIC anhydrase , *MATERIALS compression testing , *HARDNESS - Abstract
Abstract: A novel hot spring bacterial strain was isolated and characterized from Bakreshwar, West Bengal, India. Phylogenetic analysis of partial 16 S-rRNA gene sequence (697 nucleotides) suggested that the isolate was affiliated with the family ‘Firmicutes’ and 99.8% homologous with Thermoanaerobactor thermohydrosulfuricus. A unique thermostable and high pH tolerant protein (28kDa) secreted by the bacterium was isolated whose N-terminal partial sequence (25 AA) suggested that the protein was similar to mammalian carbonic anhydrase-II. This bacterium or its unique protein both increase the compressive (>25%) and tensile (>20%) strengths, hardness and elastic modulus of cementitious material when incorporated to the cement/cement–sand mixture. The protein (named as bioremediase) possesses silica leaching activity, which is increased by calcium ions. No esterase and/or proteolytic activities are present in the protein. The use of such isolated bacterium or its protein in concrete technology develops new construction material that may be used for self-healing concrete in future. [Copyright &y& Elsevier]
- Published
- 2010
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