35 results on '"Barnhart, Todd E."'
Search Results
2. Excitation function of 54Fe(p,α)51Mn from 9.5 MeV to 18 MeV
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Lin, Wilson, Wilkinson, John T., Barrett, Kendall E., Barnhart, Todd E., Gott, Matthew, Becker, Kaelyn V., Clark, Adam M., Miller, Anthony, Brown, Gunnar, DeLuca, Molly, Bartsch, Robert, Peaslee, Graham F., and Engle, Jonathan W.
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- 2022
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3. Durability test of a flowing-water target for isotope harvesting
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Abel, E. Paige, Domnanich, Katharina, Kalman, Colton, Walker, Wes, Engle, Jonathan W., Barnhart, Todd E., and Severin, Greg
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- 2020
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4. ImmunoPET imaging of tissue factor expression in pancreatic cancer with 89Zr-Df-ALT-836
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Hernandez, Reinier, England, Christopher G., Yang, Yunan, Valdovinos, Hector F., Liu, Bai, Wong, Hing C., Barnhart, Todd E., and Cai, Weibo
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- 2017
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5. Nuclear excitation functions of proton-induced reactions (Ep = 35–90 MeV) from Fe, Cu, and Al
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Graves, Stephen A., Ellison, Paul A., Barnhart, Todd E., Valdovinos, Hector F., Birnbaum, Eva R., Nortier, Francois M., Nickles, Robert J., and Engle, Jonathan W.
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- 2016
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6. Production of 34mCl and 38Cl via the (d,α) reaction on 36Ar and natAr gas at 8.4MeV
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Engle, Jonathan W., Barnhart, Todd E., DeJesus, Onofre T., and Nickles, Robert J.
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NUCLEOPHILIC reactions , *CYCLOTRONS , *ARGON isotopes , *IMAGE analysis , *BIOPHYSICS , *POSITRON emission tomography - Abstract
Abstract: Production of 38Cl and 34mCl via the (d, α) reaction on natural argon and 36Ar reveal medical cyclotrons’ ability to supply these isotopes in small quantities. 38Cl provides an inexpensive developmental tool, while 34mCl is a positron emitter of interest in PET imaging. Production yields, cyclotron target development, cryo-recovery of enriched gases, and nucleophilic chemistry of a model compound are described. [Copyright &y& Elsevier]
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- 2011
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7. PET Measurement of rCBF in the presence of a neurochemical tracer.
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Converse, Alexander K., Barnhart, Todd E., Dabbs, Kevin A., DeJesus, Onofre T., Larson, Julie A., Nickles, Robert J., Schneider, Mary L., and Roberts, Andrew D.
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NEUROTRANSMITTERS , *POSITRON emission tomography , *CEREBRAL circulation , *RHESUS monkeys , *ISOFLURANE - Abstract
Functional neurochemical imaging can indicate neurotransmitter release by detecting changes in receptor occupancy. A dual tracer positron emission tomography (PET) technique is presented here to extend such studies by simultaneously measuring changes in regional cerebral blood flow (rCBF). This would permit correlations of task or drug induced changes in rCBF and neurochemical function. In this proposed method, the rapidly varying signal from a blood flow tracer is distinguished from the slowly changing signal due to a long-lived neurochemical tracer. As a proof of principle, baseline studies were carried out in rhesus monkeys. Two monkeys were anesthetized with isoflurane, and [18F]fallypride (t1/2=110 min), a dopamine D2 receptor antagonist, was injected. Starting 99–137 min after injection, PET images were acquired every 10 s while the blood flow tracer [17F]fluoromethane (t1/2=65 s) was administered by inhalation in a repeating pattern of 45 s on/45 s off. The observed time–activity curves for 2 ml brain regions were fit with a three compartment lung–body–brain model of fluoromethane kinetics with whole brain perfusion fixed. Comparing consecutive 6 min scans, reproducibility of relative rCBF and striatal [18F]fallypride concentration were 9 and 8%, respectively. [Copyright &y& Elsevier]
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- 2004
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8. Improved production of 76Br, 77Br and 80mBr via CoSe cyclotron targets and vertical dry distillation.
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Ellison, Paul A., Olson, Aeli P., Barnhart, Todd E., Hoffman, Sabrina L.V., Reilly, Sean W., Makvandi, Mehran, Bartels, Jennifer L., Murali, Dhanabalan, DeJesus, Onofre T., Lapi, Suzanne E., Bednarz, Bryan, Nickles, Robert J., Mach, Robert H., and Engle, Jonathan W.
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CYCLOTRONS , *POLY ADP ribose , *ARYL esters , *DISTILLATION , *BORONIC esters , *RADIOACTIVE tracers , *SMALL molecules - Abstract
The radioisotopes of bromine are uniquely suitable radiolabels for small molecule theranostic radiopharmaceuticals but are of limited availability due to production challenges. Significantly improved methods were developed for the production and radiochemical isolation of clinical quality 76Br, 77Br, and 80mBr. The radiochemical quality of the radiobromine produced using these methods was tested through the synthesis of a novel 77Br-labeled inhibitor of poly (ADP-ribose) polymerase-1 (PARP-1), a DNA damage response protein. 76Br, 77Br, and 80mBr were produced in high radionuclidic purity via the proton irradiation of novel isotopically-enriched Co76Se, Co77Se, and Co80Se intermetallic targets, respectively. Radiobromine was isolated through thermal chromatographic distillation in a vertical furnace assembly. The 77Br-labeled PARP inhibitor was synthesized via copper-mediated aryl boronic ester radiobromination. Cyclotron production yields were 103 ± 10 MBq∙μA−1∙h−1 for 76Br, 88 ± 10 MBq∙μA−1∙h−1 for 80mBr at 16 MeV and 17 ± 1 MBq∙μA−1∙h−1 for 77Br at 13 MeV. Radiobromide isolation yields were 76 ± 11% in a small volume of aqueous solution. The synthesized 77Br-labeled PARP-1 inhibitor had a measured apparent molar activity up to 700 GBq/μmol at end of synthesis. A novel selenium alloy target enabled clinical-scale production of 76Br, 77Br, and 80mBr with high apparent molar activities, which was used to for the production of a new 77Br-labeled inhibitor of PARP-1. New methods for the cyclotron production and isolation of radiobromine improved the production capacity of 77Br by a factor of three and 76Br by a factor of six compared with previous methods. Preclinical translational research of 77Br-based Auger electron radiotherapeutics, such as those targeting PARP-1, will require the production of GBq-scale 77Br, which necessitates next-generation, high-yielding, isotopically-enriched cyclotron targets, such as the novel intermetallic Co77Se. [ABSTRACT FROM AUTHOR]
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- 2020
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9. Recycling of 52Cr electroplated targets for 52gMn production.
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Kretowicz, Margarita N., Barrett, Kendall E., Barnhart, Todd E., and Engle, Jonathan W.
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POSITRON emission tomography , *ELECTROPLATING , *RADIOISOTOPES - Abstract
52gMn is a promising radionuclide for positron emission tomography (PET). Enriched 52Cr targets are required to minimize formation of 54Mn radioisotopic impurities during production with proton beams. The need for radioisotopically pure 52gMn, accessibility and cost of 52Cr, sustainability of the radiochemical process, and potential for iterative purification of target materials motivate this development of recyclable, electroplated 52Cr metal targets and radiochemical isolation and labeling with resulting >99.89% radionuclidically pure 52gMn. The run-to-run replating efficiency is 60 ± 20%, and unplated chromium from this method is recovered with 94% efficiency as 52CrCl 3 hexahydrate. The decay-corrected molar activity of chemically isolated 52gMn for common chelating ligands was 376 MBq/μmol. • Manganese-52g is a promising radionuclide for positron emission tomography. • Enriched 52Cr targets are required to minimize formation of 54Mn radioisotopic impurities during production with proton beams. • Development of recyclable, electroplated 52Cr metal targets. [ABSTRACT FROM AUTHOR]
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- 2023
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10. Initial investigation of three selective and potent small molecule oxytocin receptor PET ligands in New World monkeys.
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Smith, Aaron L., Freeman, Sara M., Barnhart, Todd E., Abbott, David H., Ahlers, Elizabeth O., Kukis, David L., Bales, Karen L., Goodman, Mark M., and Young, Larry J.
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OXYTOCIN receptors , *LIGANDS (Biochemistry) , *NEUROENDOCRINE system , *UTERINE contraction , *BIOMARKERS , *POSITRON emission tomography , *LABORATORY monkeys , *PHYSIOLOGY - Abstract
The neuropeptide oxytocin is part of a neuroendocrine system that has physiological effects ranging from ensuring uterine myometrial contractions at parturition and post-partum mammary gland milk ejection to the modulation of neural control of social relationships. This initial study was performed to investigate the potential use of positron emission tomography (PET) for localizing oxytocin receptors in two New World primates. Three biomarkers for PET ( 1 – 3 ) that are known to have high affinity and selectivity for the human oxytocin receptor were investigated in the common marmoset ( Callithrix jacchus ) via PET imaging. Brain penetration, and uptake in the salivary gland area were both observed with biomarkers 2 and 3 . No brain penetration was observed with 1 , but uptake was observed more specifically in several peripheral endocrine glands compared to 2 or 3 . Biomarker 2 , which displayed the best brain penetration of the three biomarkers in the marmoset, was then investigated in the monogamous coppery titi monkey ( Callicebus cupreus ) in a brain scan and a limited full body scan. No significant brain penetration of 2 was observed in the titi monkey, but significant uptake was observed in various locations throughout the periphery. Metabolism of 2 was suspected to have been significant based upon HPLC analysis of blood draws, but parent compound was still present near the end of the scan. Follow-up investigations will focus on next generation biomarkers bearing improved binding characteristics and brain penetrability as well as investigating tissue in regions where biomarker uptake was observed. [ABSTRACT FROM AUTHOR]
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- 2016
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11. A [17F]-fluoromethane PET/TMS study of effective connectivity
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Ferrarelli, Fabio, Haraldsson, H. Magnus, Barnhart, Todd E., Roberts, Andy D., Oakes, Terrence R., Massimini, Marcello, Stone, Charles K., Kalin, Ned H., and Tononi, Giulio
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POSITRON emission tomography , *DIAGNOSTIC imaging , *MEDICAL imaging systems , *CEREBRAL cortex - Abstract
We used transcranial magnetic stimulation (TMS) in combination with positron emission tomography (PET) to investigate the effective connectivity of four cortical regions within the same study. By employing [17F]-[CH3F] ([17F]-fluoromethane) as a radiotracer of blood-flow, we were able to obtain increased sensitivity compared to [15O]-H2O for both cortical and subcortical structures. The brain areas investigated were left primary motor cortex, right primary visual cortex, and left and right prefrontal areas. We found that each site of stimulation yielded a different pattern of activation/deactivation consistent with its anatomical connectivity. Moreover, we found that TMS of prefrontal and motor cortical areas gave rise to trans-synaptic activation of subcortical circuits. [Copyright &y& Elsevier]
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- 2004
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12. Characterization of actinide resin for separation of 51,52gMn from bulk target material.
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Barrett, Kendall E., Aluicio-Sarduy, Eduardo, Happel, Steffen, Olson, Aeli P., Kutyreff, Christopher J., Ellison, Paul A., Barnhart, Todd E., and Engle, Jonathan W.
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BULK solids , *GUMS & resins , *RADIOISOTOPES , *CHROMIUM , *CYCLOTRONS - Abstract
We report an extraction chromatography-based method via Actinide Resin for the isolation of radio-manganese from both natural chromium and isotopically enriched iron targets for cyclotron production of 52gMn and 51Mn. For the separation of 52gMn from natCr, a decay-corrected radiochemical yield of 83.7 ± 8.4% was achieved. For 51Mn from 54Fe, a decay-corrected radiochemical yield of 78 ± 11% was achieved. This automatable method efficiently isolates both radionuclides from accelerator target material. [ABSTRACT FROM AUTHOR]
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- 2021
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13. Separation of cyclotron-produced cobalt-55/58m from iron targets using cation exchange chromatography with non-aqueous solvents and extraction chromatography.
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Lin, Wilson, Aluicio-Sarduy, Eduardo, Barrett, Kendall E., Barnhart, Todd E., Mixdorf, Jason C., DeLuca, Molly C., and Engle, Jonathan W.
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NONAQUEOUS solvents , *SOLVENT extraction , *IRON , *CHROMATOGRAPHIC analysis , *CYCLOTRONS , *CATIONS - Abstract
Cobalt-55 and -58m form a theranostic pair that has relevant properties for cancer research. We report a cation exchange chromatography/extraction chromatography method that separates cyclotron-produced 55/58mCo from 54/57Fe in <1.5 h, recovers >85% Co and achieves [55Co]Co-NOTA and -DOTA AMA 89 ± 48 and 35 ± 7 MBq/nmol (EOB), respectively. Cobalt-55 and -58m were quantitatively labeled to functionalized NOTA at 106 and 50 MBq/nmol (EOB), respectively, corroborating measured AMA. This method is faster than previously published methods and achieves better [55/58mCo]Co-NOTA and -DOTA AMA. • [55Co]Co-NOTA/DOTA apparent molar activity 89 ± 48/35 ± 7 MBq/nmol (N=6) at EOB. • 58mCo quantitatively labeled at 50 MBq/nmol to functionalized NOTA (EOB). • 55/58mCo separated from 54/57Fe via cation exchange and extraction chromatography in < 1.5 h. • >85% Co RCY in 1 mL and >99.99% 55Co radionuclidic purity. • >98% 54/57Fe recovery and resulting 54/57Fe can be electrodeposited. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
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14. Radiochemical isolation method for the production of 52gMn from natCr for accelerator targets.
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Barrett, Kendall E., Aluicio-Sarduy, Eduardo, Olson, Aeli P., Kutyreff, Christopher J., Ellison, Paul A., Barnhart, Todd E., Nickles, Robert J., and Engle, Jonathan W.
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CYCLOTRONS , *PRODUCTION methods - Abstract
Abstract We report a novel, precipitation-based method for the isolation of Mn from Cr targets for cyclotron production of 52gMn. The separation produces no-carrier-added 52gMn with a decay corrected radiochemical yield of 85 ± 3% and apparent molar activity for DOTA of 1.3 GBq/μmol. This method reduces stable metallic impurities in the purified 52gMn compared to previously reported chromatographic methods. Highlights • Radiochemical separation using a 2-phase precipitation method and single extraction chromatography column. • Separation strategy for production no-carrier-added 52gMn. • Process allows for chelation studies with DOTA. [ABSTRACT FROM AUTHOR]
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- 2019
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15. Simplified and automatable radiochemical separation strategy for the production of radiopharmaceutical quality 86Y using single column extraction chromatography.
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Aluicio-Sarduy, Eduardo, Hernandez, Reinier, Valdovinos, Hector F., Kutyreff, Christopher J., Ellison, Paul A., Barnhart, Todd E., Nickles, Robert J., and Engle, Jonathan W.
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RADIOCHEMICAL separation , *RADIOPHARMACEUTICALS , *CANCER treatment , *COMPANION diagnostics , *CHROMATOGRAPHIC analysis - Abstract
Abstract We present a simplified, automatable single-column radiochemical separation method using the extraction chromatographic branched-DGA resin for the production of no-carrier-added 86Y with a radiochemical yield higher than 95%, an apparent molar activity of 1.4 ± 0.4 Ci/μmol (DOTA) and 2.3 ± 0.7 Ci/μmol (DTPA), and a run-to-run recycling efficiency of the isotopically-enriched target of 98 ± 1%. These results enable the preparation of 86Y radiopharmaceuticals for 86Y/90Y-based cancer theranostic applications. Highlights • Simple radiochemical separation using single column extraction chromatography. • Separation strategy for production of high-quality theranostic Y-86 applications. • Process allows for high recycling efficiency of the enriched target material. [ABSTRACT FROM AUTHOR]
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- 2018
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16. Evaluation of a chloride-based 89Zr isolation strategy using a tributyl phosphate (TBP)-functionalized extraction resin.
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Graves, Stephen A., Kutyreff, Christopher, Barrett, Kendall E., Hernandez, Reinier, Ellison, Paul A., Happel, Steffen, Aluicio-Sarduy, Eduardo, Barnhart, Todd E., Nickles, Robert J., and Engle, Jonathan W.
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ZIRCONIUM , *TRIBUTYL phosphate , *EXTRACTION (Chemistry) , *TRACE metals , *CHLORIDES - Abstract
Abstract Introduction The remarkable stability of the 89Zr-DOTA complex has been shown in recent literature. The formation of this complex appears to require 89Zr-chloride as the complexation precursor rather than the more conventional 89Zr-oxalate. In this work we present a method for the direct isolation of 89Zr-chloride from irradiated natY foils. Methods 89Zr, 88Zr, and 88Y were prepared by 16 MeV proton irradiation of natY foils and used for batch-extraction based equilibrium coefficient measurements for TBP and UTEVA resin. Radionuclidically pure 89Zr was prepared by 14 MeV proton-irradiation of natY foils. These foils were dissolved in concentrated HCl, trapped on columns of TBP or UTEVA resin, and 89Zr-chloride was eluted in <1 mL of 0.1 M HCl. For purposes of comparison, conventionally-isolated 89Zr-oxalate was converted to 89Zr-chloride by trapping, rinsing, and elution from a QMA cartridge into 1 M HCl. Trace metal analysis was performed on the resulting 89Zr products. Results Equilibrium coefficients for Y and Zr were similar between UTEVA and TBP resins across all HCl concentrations. K d values of <10−1 mL/g were observed for Y across all HCl concentrations. K d values of >103 mL/g were observed at HCl concentrations >9 M for Zr, falling to K d values of <100 mL/g at low HCl concentrations. 89Zr-chloride was recovered from small columns of TBP in <1 mL of 0.1 M HCl with an overall recovery efficiency of 89 ± 3% (n = 3). An average Y/Zr separation factor of 1.5 × 105 (n = 3) was obtained. Trace metal impurities, notably Fe, were higher in TBP-isolated 89Zr-chloride compared with 89Zr-chloride prepared using the conventional two-step procedure. Conclusion TBP-functionalized resin appears promising for the direct isolation of 89Zr-chloride from irradiated natY targets. Excellent 89Zr recovery efficiencies were obtained, and chemical purity was sufficient for proof-of-concept chelation studies. [ABSTRACT FROM AUTHOR]
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- 2018
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17. Production and radiochemistry of antimony-120m: Efforts toward Auger electron therapy with 119Sb.
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Kostelnik, Thomas I., Olson, Aeli P., Grundmane, Aivija, Ellison, Paul A., Mynerich, Jenasee, Chen, Shaohuang, Marinova, Atanaska, Randhawa, Parmissa, Karaivanov, Dimitar, Aluicio-Sarduy, Eduardo, Barnhart, Todd E., Orvig, Chris, Ramogida, Caterina F., Hoehr, Cornelia, Filosofov, Dmitry, Engle, Jonathan W., and Radchenko, Valery
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RADIOCHEMISTRY , *PARTICLE emissions , *RADIOCHEMICAL purification , *ELECTRONS , *RADIOISOTOPES , *ELECTRON emission - Abstract
Targeted Meitner-Auger Therapy (TMAT) has potential for personalized treatment thanks to its subcellular dosimetric selectivity, which is distinct from the dosimetry of β − and α particle emission based Targeted Radionuclide Therapy (TRT). To date, most clinical and preclinical TMAT studies have used commercially available radionuclides. These studies showed promising results despite using radionuclides with theoretically suboptimal photon to electron ratios, decay kinetics, and electron emission spectra. Studies using radionuclides whose decay characteristics are considered more optimal are therefore important for evaluation of the full potential of Meitner-Auger therapy; 119Sb is among the best such candidates. In the present work, we develop radiochemical purification of 120Sb from irradiated natural tin targets for TMAT studies with 119Sb. [Display omitted] [ABSTRACT FROM AUTHOR]
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- 2023
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18. Radiolabeled polyoxometalate clusters: Kidney dysfunction evaluation and tumor diagnosis by positron emission tomography imaging.
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Ni, Dalong, Jiang, Dawei, Im, Hyung-Jun, Valdovinos, Hector F., Yu, Bo, Goel, Shreya, Barnhart, Todd E., Huang, Peng, and Cai, Weibo
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POLYOXOMETALATES , *KIDNEY tumors , *TUMOR microenvironment , *POSITRON emission tomography , *RADIOLABELING , *DIAGNOSIS - Abstract
Radiolabeled nanoprobes for positron emission tomography (PET) imaging has received special attention over the past decade, allowing for sensitive, non-invasive, and quantitative detection of different diseases. The rapidly renal clearable nanomaterials normally suffer from a low accumulation in the tumor through the enhanced permeability and retention (EPR) effect due to the rapidly reduced concentration in the blood circulation after renal clearance. It is highly important to design radiolabeled nanomaterials which can meet the balance between the rapid renal clearance and strong EPR effect within a suitable timescale. Herein, renal clearable polyoxometalate (POM) clusters of ultra-small size (∼1 nm in diameter) were readily radiolabeled with the oxophilic 89 Zr to obtain 89 Zr-POM clusters, which may allow for efficient staging of kidney dysfunction in a murine model of unilateral ureteral obstruction (UUO). Furthermore, the as-synthesized clusters can accumulate in the tumor through EPR effect and self-assemble into larger nanostructures in the acidic tumor microenvironment for enhanced tumor accumulation, offering an excellent balance between renal clearance and EPR effect. [ABSTRACT FROM AUTHOR]
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- 2018
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19. Bacteria-like mesoporous silica-coated gold nanorods for positron emission tomography and photoacoustic imaging-guided chemo-photothermal combined therapy.
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Xu, Cheng, Chen, Feng, Valdovinos, Hector F., Jiang, Dawei, Goel, Shreya, Yu, Bo, Sun, Haiyan, Barnhart, Todd E., Moon, James J., and Cai, Weibo
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MESOPOROUS silica , *DRUG delivery devices , *CANCER treatment , *NANOMEDICINE , *COMBINATION drug therapy , *PHOTOTHERAPY - Abstract
Mesoporous silica nanoshell (MSN) coating has been demonstrated as a versatile surface modification strategy for various kinds of inorganic functional nanoparticles, such as gold nanorods (GNRs), to achieve not only improved nanoparticle stability but also concomitant drug loading capability. However, limited drug loading capacity and low tumor accumulation rate in vivo are two major challenges for the biomedical applications of MSN-coated GNRs (GNR@MSN). In this study, by coating uniformly sized GNRs with MSN in an oil-water biphase reaction system, we have successfully synthesized a new bacteria-like GNR@MSN (i.e., bGNR@MSN) with a significantly enlarged pore size (4–8 nm) and surface area (470 m 2 /g). After PEGylation and highly efficient loading of doxorubicin (DOX, 40.9%, w/w), bGNR@MSN were used for positron emission tomography (PET, via facile and chelator-free 89 Zr-labeling) and photoacoustic imaging-guided chemo-photothermal cancer therapy in vivo . PET imaging showed that 89 Zr-labeled bGNR@MSN(DOX)-PEG can passively target to the 4T1 murine breast cancer-bearing mice with high efficiency (∼10 %ID/g), based on enhanced permeability and retention effect. Significantly enhanced chemo-photothermal combination therapy was also achieved due to excellent photothermal effect and near-infrared-light-triggered drug release by bGNR@MSN(DOX)-PEG at the tumor site. The promising results indicate great potential of bGNR@MSN-PEG nanoplatforms for future cancer diagnosis and therapy. [ABSTRACT FROM AUTHOR]
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- 2018
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20. Characterization of the radiosynthesis and purification of [18F]THK-5351, a PET ligand for neurofibrillary tau.
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Betthauser, Tobey J., Ellison, Paul A., Murali, Dhanabalan, Lao, Patrick J., Barnhart, Todd E., Furumoto, Shozo, Okamura, Nobuyuki, Johnson, Sterling C., Engle, Jonathan W., Nickles, Robert J., and Christian, Bradley T.
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HIGH performance liquid chromatography , *SOLID phase extraction , *CHROMATOGRAMS , *RADIOACTIVITY , *ALZHEIMER'S disease - Abstract
This work characterizes the radiochemical synthesis, purification, and formulation of [ 18 F]THK-5351, a tau PET radioligand, and develops an automated radiosynthesis routine (ELIXYS, Sofie Biosciences). Nucleophilic radiofluorination reaction was complete by 7 min at 110 °C with radiochemical yields proportional to precursor mass (0.1–0.5 mg). Optimized HPLC purification produced radiotracer product with no chemical impurities observed on analytical HPLC in formulation. Automated radiosynthesis (ELIXYS), HPLC purification and formulation was completed in 86 min producing formulated product suitable for human research use. [ABSTRACT FROM AUTHOR]
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- 2017
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21. Theranostic cobalt-55/58m for neurotensin receptor-mediated radiotherapy in vivo: A pilot study with dosimetry.
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Lin, Wilson, Aluicio-Sarduy, Eduardo, Houson, Hailey A., Barnhart, Todd E., Tekin, Volkan, Jeffery, Justin J., Weichmann, Ashley M., Barrett, Kendall E., Lapi, Suzanne E., and Engle, Jonathan W.
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MEDICAL dosimetry , *NEUROTENSIN , *BLOOD cell count , *NUDITY , *ALPHA rays , *RADIOCHEMICAL purification - Abstract
Neurotensin receptor 1 (NTSR1) can stimulate tumor proliferation through neurotensin (NTS) activation and are overexpressed by a variety of cancers. The high binding affinity of NTS/NTSR1 makes radiolabeled NTS derivatives interesting for cancer diagnosis and staging. Internalization of NTS/NTSR1 also suggests therapeutic application with high LET alpha particles and low energy electrons. We investigated the therapeutic efficacy of [58mCo]Co-NOTA-NT-20.3 in vivo using murine models xenografted with NTSR1-positive HT29 human colorectal adenocarcinoma cells, and utilized [55Co]Co-NOTA-NT-20.3 for dosimetry. Targeting properties and cytotoxicity of [55/58mCo]Co-NOTA-NT-20.3 were assessed with HT29 cells. Female nude mice were xenografted with HT29 tumors and administered [55Co or 58mCo]Co-NOTA-NT-20.3 to evaluate pharmacokinetics or for therapy, respectively. Dosimetry calculations followed the Medical Internal Radiation Dose (MIRD) formalism and human absorbed dose rate per unit activity were obtained from OpenDose. The pilot therapy study consisted of two groups (each N = 3) receiving 110 ± 15 MBq and 26 ± 6 MBq [58mCo]Co-NOTA-NT-20.3 one week after tumor inoculation, and control (N = 3). Tumor sizes and masses were measured twice a week after therapy. Complete blood count and kidney histology were also performed to assess toxicity. HPLC measured radiochemical purity of [55,58mCo]Co-NOTA-NT-20.3 > 99 %. Labeled compounds retained NTS targeting properties. [58mCo]Co-NOTA-NT-20.3 exhibited cytotoxicity for HT29 cells and was >15× more potent than [58mCo]CoCl 2. Xenografted tumors responded modestly to administered doses, but mice showed no signs of radiotoxicity. Absorbed dose to tumor and kidney with 110 MBq [58mCo]Co-NOTA-NT-20.3 were 0.6 Gy and 0.8 Gy, respectively, and other organs received less than half of the absorbed dose to tumor. Off-target radiation dose from cobalt-58g was small but reduces the therapeutic window. The enhanced in vitro cytotoxicity and high tumor-to-background led us to investigate the therapeutic efficacy of [58mCo]Co-NOTA-NT-20.3 in vivo. Although we were unable to induce tumor response commensurate with [177Lu]Lu-NT127 (NLys-Lys-Pro-Tyr-Tle-Leu) studies involving similar time-integrated activity, the absence of observed toxicity may constitute an opportunity for targeting vectors with improved uptake and/or retention to avoid the aftereffects of other high-LET radioactive emissions. Future studies with higher uptake, activity and/or multiple dosing regimens are warranted. The theranostic approach employed in this work was crucial for dosimetry analysis. [Display omitted] • First in vivo therapy study of 58mCo with an internalizing targeting vector • [58mCo]Co-NOTA-NT-20.3 was >15× more cytotoxic to HT29 cells in vitro than [58mCo]CoCl 2. • Theranostic approach with 55Co enabled visualization of tumor uptake and dosimetry. [ABSTRACT FROM AUTHOR]
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- 2023
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22. Spot-welding solid targets for high current cyclotron irradiation.
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Ellison, Paul A., Valdovinos, Hector F., Graves, Stephen A., Barnhart, Todd E., and Nickles, Robert J.
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SPOT welding , *POSITRON emission , *POSITRON emission tomography , *ZIRCONIUM , *TITANIUM group - Abstract
Zirconium-89 finds broad application for use in positron emission tomography. Its cyclotron production has been limited by the heat transfer from yttrium targets at high beam currents. A spot welding technique allows a three-fold increase in beam current, without affecting 89 Zr quality. An yttrium foil, welded to a jet-cooled tantalum support base accommodates a 50 µA proton beam degraded to 14 MeV. The resulting activity yield of 48±4 MBq/(μA∙hr) now extends the outreach of 89 Zr for a broader distribution. [ABSTRACT FROM AUTHOR]
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- 2016
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23. VEGFR targeting leads to significantly enhanced tumor uptake of nanographene oxide in vivo.
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Shi, Sixiang, Yang, Kai, Hong, Hao, Chen, Feng, Valdovinos, Hector F., Goel, Shreya, Barnhart, Todd E., Liu, Zhuang, and Cai, Weibo
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VASCULAR endothelial growth factors , *TARGETED drug delivery , *GRAPHENE oxide , *TUMOR treatment , *NANOSTRUCTURED materials , *POSITRON emission tomography - Abstract
Although graphene oxide (GO) has recently been considered as a highly attractive nanomaterial for future cancer imaging and therapy, it is still a major challenge to improve its in vivo tumor active targeting efficiency. Here in this full article, we demonstrated the successful and significantly enhanced in vivo tumor vasculature targeting efficacy of well-functionalized GO nanoconjugates by using vascular endothelial growth factor 121 (VEGF121) as the targeting ligand. As-developed GO nanoconjugate exhibits excellent in vivo stability, specific in vitro and in vivo vascular endothelial growth factor receptor (VEGFR) targeting, significantly enhanced tumor accumulation (>8 %ID/g) as well as high tumor-to-muscle contrast, showing great potential for future tumor targeted imaging and therapy. [ABSTRACT FROM AUTHOR]
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- 2015
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24. The effects of chronic alcohol self-administration on serotonin-1A receptor binding in nonhuman primates.
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Hillmer, Ansel T., Wooten, Dustin W., Tudorascu, Dana L., Barnhart, Todd E., Ahlers, Elizabeth O., Resch, Leslie M., Larson, Julie A., Converse, Alexander K., Moore, Colleen F., Schneider, Mary L., and Christian, Bradley T.
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PHYSIOLOGICAL effects of alcohol , *SEROTONIN receptors , *POSITRON emission tomography , *RADIOLIGAND assay , *BRAIN imaging , *ALCOHOLISM , *ETHANOL - Abstract
Background Previous studies have found interrelationships between the serotonin system and alcohol self-administration. The goal of this work was to directly observe in vivo effects of chronic ethanol self-administration on serotonin 5-HT 1A receptor binding with [ 18 F]mefway PET neuroimaging in rhesus monkeys. Subjects were first imaged alcohol-naïve and again during chronic ethanol self-administration to quantify changes in 5-HT 1A receptor binding. Methods Fourteen rhesus monkey subjects (10.7–12.8 years) underwent baseline [ 18 F]mefway PET scans prior to alcohol exposure. Subjects then drank gradually increasing ethanol doses over four months as an induction period, immediately followed by at least nine months ad libidum ethanol access. A post [ 18 F]mefway PET scan was acquired during the final three months of ad libidum ethanol self-administration. 5-HT 1A receptor binding was assayed with binding potential ( BP ND ) using the cerebellum as a reference region. Changes in 5-HT 1A binding during chronic ethanol self-administration were examined. Relationships of binding metrics with daily ethanol self-administration were also assessed. Results Widespread increases in 5-HT 1A binding were observed during chronic ethanol self-administration, independent of the amount of ethanol consumed. A positive correlation between 5-HT 1A binding in the raphe nuclei and average daily ethanol self-administration was also observed, indicating that baseline 5-HT 1A binding in this region predicted drinking levels. Conclusions The increase in 5-HT 1A binding levels during chronic ethanol self-administration demonstrates an important modulation of the serotonin system due to chronic alcohol exposure. Furthermore, the correlation between 5-HT 1A binding in the raphe nuclei and daily ethanol self-administration indicates a relationship between the serotonin system and alcohol self-administration. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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25. Changes in the α4β2* nicotinic acetylcholine system during chronic controlled alcohol exposure in nonhuman primates.
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Hillmer, Ansel T, Tudorascu, Dana L, Wooten, Dustin W, Lao, Patrick J, Barnhart, Todd E, Ahlers, Elizabeth O, Resch, Leslie M, Larson, Julie A, Converse, Alexander K, Moore, Colleen F, Schneider, Mary L, and Christian, Bradley T
- Abstract
Background: The precise nature of modifications to the nicotinic acetylcholine receptor (nAChR) system in response to chronic ethanol exposure is poorly understood. The present work used PET imaging to assay α4β2* nAChR binding levels of eight rhesus monkeys before and during controlled chronic ethanol intake.Methods: [(18)F]Nifene PET scans were conducted prior to alcohol exposure, and then again after at least 8 months controlled ethanol exposure, including 6 months at 1.5 g/kg/day following a dose escalation period. Receptor binding levels were quantified with binding potentials (BPND) using the cerebellum as a reference region. Alcohol self-administration was assessed as average daily alcohol intake during a 2 month free drinking period immediately following controlled alcohol.Results: Significant decreases in α4β2* nAChR binding were observed in both frontal and insular cortex in response to chronic ethanol exposure. During chronic alcohol exposure, BPND in the lateral geniculate region correlated positively with the amount of alcohol consumed during free drinking.Conclusions: The observed decreases in nAChR availability following chronic alcohol consumption suggest alterations to this receptor system in response to repeated alcohol administration, making this an important target for further study in alcohol abuse and alcohol and nicotine codependence. [ABSTRACT FROM AUTHOR]- Published
- 2014
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26. Amyloid burden and neural function in people at risk for Alzheimer's Disease.
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Johnson, Sterling C., Christian, Bradley T., Okonkwo, Ozioma C., Oh, Jennifer M., Harding, Sandra, Xu, Guofan, Hillmer, Ansel T., Wooten, Dustin W., Murali, Dhanabalan, Barnhart, Todd E., Hall, Lance T., Racine, Annie M., Klunk, William E., Mathis, Chester A., Bendlin, Barbara B., Gallagher, Catherine L., Carlsson, Cynthia M., Rowley, Howard A., Hermann, Bruce P., and Dowling, N. Maritza
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AMYLOID beta-protein , *ALZHEIMER'S disease risk factors , *BRAIN imaging , *POSITRON emission tomography , *COGNITIVE testing , *MAGNETIC resonance imaging , *CEREBRAL atrophy - Abstract
Abstract: To determine the relationship between amyloid burden and neural function in healthy adults at risk for Alzheimer's Disease (AD), we used multimodal imaging with [C-11]Pittsburgh compound B positron emission tomography, [F-18]fluorodeoxyglucose, positron emission tomography , and magnetic resonance imaging, together with cognitive measurement in 201 subjects (mean age, 60.1 years; range, 46–73 years) from the Wisconsin Registry for Alzheimer's Prevention. Using a qualitative rating, 18% of the samples were strongly positive Beta-amyloid (Aβ+), 41% indeterminate (Aβi), and 41% negative (Aβ−). Aβ+ was associated with older age, female sex, and showed trends for maternal family history of AD and APOE4. Relative to the Aβ− group, Aβ+ and Aβi participants had increased glucose metabolism in the bilateral thalamus; Aβ+ participants also had increased metabolism in the bilateral superior temporal gyrus. Aβ+ participants exhibited increased gray matter in the lateral parietal lobe bilaterally relative to the Aβ− group, and no areas of significant atrophy. Cognitive performance and self report cognitive and affective symptoms did not differ between groups. Amyloid burden can be identified in adults at a mean age of 60 years and is accompanied by glucometabolic increases in specific areas, but not atrophy or cognitive loss. This asymptomatic stage may be an opportune window for intervention to prevent progression to symptomatic AD. [Copyright &y& Elsevier]
- Published
- 2014
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27. Image-guided and tumor-targeted drug delivery with radiolabeled unimolecular micelles.
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Guo, Jintang, Hong, Hao, Chen, Guojun, Shi, Sixiang, Zheng, Qifeng, Zhang, Yin, Theuer, Charles P., Barnhart, Todd E., Cai, Weibo, and Gong, Shaoqin
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DRUG delivery systems , *MICELLES , *RADIOACTIVE tracers , *BLOCK copolymers , *AMPHIPHILES , *DENDRITIC cells , *ENDOTHELIAL cells - Abstract
Abstract: Unimolecular micelles formed by dendritic amphiphilic block copolymers poly(amidoamine)–poly(l-lactide)-b-poly(ethylene glycol) conjugated with anti-CD105 monoclonal antibody (TRC105) and 1,4,7-triazacyclononane-N, N′, N-triacetic acid (NOTA, a macrocyclic chelator for 64Cu) (abbreviated as PAMAM–PLA-b-PEG–TRC105) were synthesized and characterized. Doxorubicin (DOX), a model anti-cancer drug, was loaded into the hydrophobic core of the unimolecular micelles formed by PAMAM and PLA via physical encapsulation. The unimolecular micelles exhibited a uniform size distribution and pH-sensitive drug release behavior. TRC105-conjugated unimolecular micelles showed a CD105-associated cellular uptake in human umbilical vein endothelial cells (HUVEC) compared with non-targeted unimolecular micelles, which was further validated by cellular uptake in CD105-negative MCF-7 cells. In 4T1 murine breast tumor-bearing mice, 64Cu-labeled targeted micelles exhibited a much higher level of tumor accumulation than 64Cu-labeled non-targeted micelles, measured by serial non-invasive positron emission tomography (PET) imaging and confirmed by biodistribution studies. These unimolecular micelles formed by dendritic amphiphilic block copolymers that synergistically integrate passive and active tumor-targeting abilities with pH-controlled drug release and PET imaging capabilities provide the basis for future cancer theranostics. [Copyright &y& Elsevier]
- Published
- 2013
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28. Prenatal Stress Induces Increased Striatal Dopamine Transporter Binding in Adult Nonhuman Primates.
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Converse, Alexander K., Moore, Colleen F., Moirano, Jeffrey M., Ahlers, Elizabeth O., Larson, Julie A., Engle, Jonathan W., Barnhart, Todd E., Murali, Dhanabalan, Christian, Bradley T., DeJesus, Onofre T., Holden, James E., Nickles, Robert J., and Schneider, Mary L.
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PRENATAL care , *PHYSIOLOGICAL stress , *DOPAMINE , *POSITRON emission tomography , *PRIMATE physiology , *ALCOHOL use in pregnancy , *BEHAVIORAL assessment - Abstract
Background: To determine the effects in adult offspring of maternal exposure to stress and alcohol during pregnancy, we imaged striatal and midbrain dopamine transporter (DAT) binding by positron emission tomography in rhesus monkeys (Macaca mulatta). We also evaluated the relationship between DAT binding and behavioral responses previously found to relate to dopamine D2 receptor density (responsivity to tactile stimuli, performance on a learning task, and behavior during a learning task). Methods: Subjects were adult offspring derived from a 2 × 2 experiment in which pregnant monkeys were randomly assigned to control, daily mild stress exposure (acoustic startle), voluntary consumption of moderate-level alcohol, or both daily stress and alcohol. Adult offspring (n = 38) were imaged by positron emission tomography with the DAT ligand [18F]2β-carbomethoxy-3β-(4-chlorophenyl)-8-(2-fluoroethyl)-nortropane ([18F]FECNT). Results: Results showed that prenatal stress yielded an overall increase of 15% in [18F]FECNT binding in the striatum (p = .016), 17% greater binding in the putamen (p = .012), and 13% greater binding in the head of the caudate (p = .028) relative to animals not exposed to prenatal stress. Striatal [18F]FECNT binding correlated negatively with habituation to repeated tactile stimulation and positively with tactile responsivity. There were no significant effects of prenatal alcohol exposure on [18F]FECNT binding. Conclusions: Maternal exposure to mild daily stress during pregnancy yielded increases in striatal DAT availability that were apparent in adult offspring and were associated with behavioral characteristics reflecting tactile hyperresponsivity, a condition associated with problem behaviors in children. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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29. Alternative strategies for the synthesis of [11C]ER176 for PET imaging of neuroinflammation.
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Mixdorf, Jason C., Murali, Dhanabalan, Xin, Yangchun, DiFilippo, Alexandra H., Aluicio-Sarduy, Eduardo, Barnhart, Todd E., Engle, Jonathan W., Ellison, Paul A., and Christian, Bradley T.
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POSITRON emission tomography , *NEUROINFLAMMATION , *SOLID phase extraction , *RADIOCHEMICAL purification , *HIGH performance liquid chromatography - Abstract
[11C]ER176 is a next generation PET radioligand for imaging 18 kDa translocator protein, a biomarker for neuroinflammation. The goal of this work was to investigate alternative strategies for the radiochemical synthesis, purification, and formulation of [11C]ER176. An optimized tri-solvent high-performance liquid chromatography (HPLC) protocol is described to separate the hydro-de-chlorinated byproduct from [11C]ER176. A newly implemented solid phase extraction work-up efficiently removed HPLC solvent while maintaining chemical purity and overall radiochemical yield and purity. This new HPLC purification and final formulation was completed within 40 min, providing 2.7 ± 0.5 GBq of [11C]ER176 at end of synthesis with 1400 ± 300 GBq/μmol molar activity while meeting all specifications for radiopharmaceutical quality control tests for human research use. • New ternary isocratic semi-preparative HPLC providing improved resolution of [11C]ER176 from non-radioactive impurity. • Identification of non-radioactive impurity that elutes proximal to [11C]ER176 and preliminary mechanistic investigation of its formation. • Purification of [11C]ER176 utilizing solid-phase extraction (SPE) for removal of semi-preparatory HPLC mobile phase. • Production of [11C]ER176 within 40 min, providing 2.7 ± 0.5 GBq at end of synthesis with 1400 ± 300 GBq/μmol molar activity. [ABSTRACT FROM AUTHOR]
- Published
- 2021
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- View/download PDF
30. The effects of lobeline on α4β2* nicotinic acetylcholine receptor binding and uptake of [18F]nifene in rats
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Hillmer, Ansel T., Wooten, Dustin W., Farhoud, Mohammed, Barnhart, Todd E., Mukherjee, Jogeshwar, and Christian, Bradley T.
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NICOTINIC acetylcholine receptors , *SMOKING cessation , *BLOOD-brain barrier , *AMINES , *POSITRON emission tomography , *LABORATORY rats - Abstract
Abstract: Lobeline is a potential smoking cessation drug with affinity for the α4β2 nicotinic acetylcholine receptor and may inhibit the blood–brain barrier (BBB) amine transporter. The goal of this work was to use PET imaging to evaluate the effects of lobeline on the kinetic properties of [18F]nifene in the rat brain. Methods: Direct α4β2* competition of lobeline with [18F]nifene was evaluated using imaging experiments with both displacing and blocking doses of lobeline (1mg/kg, i.v.) given between two injections of [18F]nifene separated by 50min. Inhibition of the BBB amine transporter was examined using a separate imaging protocol with three injections of [18F]nifene, first at baseline, then following (−)nicotine blocking, and finally following lobeline blocking. Results: Rapid displacement of [18F]nifene was observed in the α4β2*-rich thalamus following lobeline administration, suggesting direct competition of the drug at α4β2* sites. Slight decreases in BBB transport of [18F]nifene were observed when the α4β2* system was first saturated with (−)nicotine and then given lobeline. This perturbation may be due to inhibition of the BBB amine transporter by lobeline or reductions in blood flow. Significant cerebellar displacement of [18F]nifene was found following the administration of both lobeline and (−)nicotine, indicating detectable specific binding in the rat cerebellum. Conclusion: The competition of lobeline with [18F]nifene is largely dominated at the α4β2* binding site and only small perturbations in BBB transport of [18F]nifene are seen at the 1mg/kg dose. Similar experiments could be used to study other drugs as therapeutic agents for smoking cessation with PET. [Copyright &y& Elsevier]
- Published
- 2013
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31. Tumor vasculature targeting and imaging in living mice with reduced graphene oxide
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Shi, Sixiang, Yang, Kai, Hong, Hao, Valdovinos, Hector F., Nayak, Tapas R., Zhang, Yin, Theuer, Charles P., Barnhart, Todd E., Liu, Zhuang, and Cai, Weibo
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TUMOR treatment , *BLOOD vessels , *TARGETED drug delivery , *LABORATORY mice , *GRAPHENE , *NANOSTRUCTURED materials , *POSITRON emission tomography - Abstract
Abstract: Graphene-based nanomaterials have attracted tremendous attention in the field of biomedicine due to their intriguing properties. Herein, we report tumor vasculature targeting and imaging in living mice using reduced graphene oxide (RGO), which was conjugated to the anti-CD105 antibody TRC105. The RGO conjugate, 64Cu–NOTA–RGO–TRC105, exhibited excellent stability in vitro and in vivo. Serial positron emission tomography (PET) imaging studies non-invasively assessed the pharmacokinetics and demonstrated specific targeting of 64Cu–NOTA–RGO–TRC105 to 4T1 murine breast tumors in vivo, compared to non-targeted RGO conjugate (64Cu–NOTA–RGO). In vivo (e.g., blocking 4T1 tumor uptake with excess TRC105), in vitro (e.g., flow cytometry), and ex vivo (e.g., histology) experiments confirmed the specificity of 64Cu–NOTA–RGO–TRC105 for tumor vascular CD105. Since RGO exhibits desirable properties for photothermal therapy, the tumor-specific RGO conjugate developed in this work may serve as a promising theranostic agent that integrates imaging and therapeutic components. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
32. In vivo targeting and positron emission tomography imaging of tumor vasculature with 66Ga-labeled nano-graphene
- Author
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Hong, Hao, Zhang, Yin, Engle, Jonathan W., Nayak, Tapas R., Theuer, Charles P., Nickles, Robert J., Barnhart, Todd E., and Cai, Weibo
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IMAGING of cancer , *POSITRON emission tomography , *GRAPHENE , *NANOSTRUCTURED materials , *GALLIUM , *TARGETED drug delivery , *ANIMAL models of cancer , *PHARMACOKINETICS - Abstract
Abstract: The goal of this study was to employ nano-graphene for tumor targeting in an animal tumor model, and quantitatively evaluate the pharmacokinetics and tumor targeting efficacy through positron emission tomography (PET) imaging using 66Ga as the radiolabel. Nano-graphene oxide (GO) sheets with covalently linked, amino group-terminated six-arm branched polyethylene glycol (PEG; 10 kDa) chains were conjugated to NOTA (1,4,7-triazacyclononane-1,4,7-triacetic acid, for 66Ga-labeling) and TRC105 (an antibody that binds to CD105). Flow cytometry analyses, size measurements, and serum stability studies were performed to characterize the GO conjugates before in vivo investigations in 4T1 murine breast tumor-bearing mice, which were further validated by histology. TRC105-conjugated GO was specific for CD105 in cell culture. 66Ga-NOTA-GO-TRC105 and 66Ga-NOTA-GO exhibited excellent stability in complete mouse serum. In 4T1 tumor-bearing mice, these GO conjugates were primarily cleared through the hepatobiliary pathway. 66Ga-NOTA-GO-TRC105 accumulated quickly in the 4T1 tumors and tumor uptake remained stable over time (3.8 ± 0.4, 4.5 ± 0.4, 5.8 ± 0.3, and 4.5 ± 0.4 %ID/g at 0.5, 3, 7, and 24 h post-injection respectively; n = 4). Blocking studies with unconjugated TRC105 confirmed CD105 specificity of 66Ga-NOTA-GO-TRC105, which was corroborated by biodistribution and histology studies. Furthermore, histological examination revealed that targeting of NOTA-GO-TRC105 is tumor vasculature CD105 specific with little extravasation. Successful demonstration of in vivo tumor targeting with GO, along with the versatile chemistry of graphene-based nanomaterials, makes them suitable nanoplatforms for future biomedical research such as cancer theranostics. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
33. Multifunctional unimolecular micelles for cancer-targeted drug delivery and positron emission tomography imaging
- Author
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Xiao, Yuling, Hong, Hao, Javadi, Alireza, Engle, Jonathan W., Xu, Wenjin, Yang, Yunan, Zhang, Yin, Barnhart, Todd E., Cai, Weibo, and Gong, Shaoqin
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MICELLES , *DRUG delivery systems , *POSITRON emission tomography , *COPOLYMERS , *LABORATORY mice , *PEPTIDES , *INTEGRINS - Abstract
Abstract: A multifunctional unimolecular micelle made of a hyperbranched amphiphilic block copolymer was designed, synthesized, and characterized for cancer-targeted drug delivery and non-invasive positron emission tomography (PET) imaging in tumor-bearing mice. The hyperbranched amphiphilic block copolymer, Boltorn® H40-poly(L-glutamate-hydrazone-doxorubicin)-b-poly(ethylene glycol) (i.e., H40-P(LG-Hyd-DOX)-b-PEG), was conjugated with cyclo(Arg-Gly-Asp-D-Phe-Cys) peptides (cRGD, for integrin αvβ3 targeting) and macrocyclic chelators (1,4,7-triazacyclononane-N, N’, N’’-triacetic acid [NOTA], for 64Cu-labeling and PET imaging) (i.e., H40-P(LG-Hyd-DOX)-b-PEG-OCH3/cRGD/NOTA, also referred to as H40-DOX-cRGD). The anti-cancer drug, doxorubicin (DOX) was covalently conjugated onto the hydrophobic segments of the amphiphilic block copolymer arms (i.e., PLG) via a pH-labile hydrazone linkage to enable pH-controlled drug release. The unimolecular micelles exhibited a uniform size distribution and pH-sensitive drug release behavior. cRGD-conjugated unimolecular micelles (i.e., H40-DOX-cRGD) exhibited a much higher cellular uptake in U87MG human glioblastoma cells due to integrin αvβ3-mediated endocytosis than non-targeted unimolecular micelles (i.e., H40-DOX), thereby leading to a significantly higher cytotoxicity. In U87MG tumor-bearing mice, H40-DOX-cRGD-64Cu also exhibited a much higher level of tumor accumulation than H40-DOX-64Cu, measured by non-invasive PET imaging and confirmed by biodistribution studies and ex vivo fluorescence imaging. We believe that unimolecular micelles formed by hyperbranched amphiphilic block copolymers that synergistically integrate passive and active tumor-targeting abilities with pH-controlled drug release and PET imaging capabilities provide the basis for future cancer theranostics. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
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34. Paraquat is excluded by the blood brain barrier in rhesus macaque: An in vivo pet study
- Author
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Bartlett, Rachel M., Holden, James E., Nickles, R. Jerome, Murali, Dhanabalan, Barbee, David L., Barnhart, Todd E., Christian, Bradley T., and DeJesus, Onofre T.
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PARAQUAT , *BLOOD-brain barrier , *RHESUS monkeys , *PARKINSON'S disease , *ETIOLOGY of diseases , *NEUROTOXICOLOGY , *METHYLPHENYLTETRAHYDROPYRIDINE , *DOPAMINE receptors , *ENVIRONMENTALLY induced diseases - Abstract
Abstract: Environmental factors have long been thought to have a role in the etiology of idiopathic Parkinson''s disease (PD). Since the discovery of the selective neurotoxicity of MPTP to dopamine cells, suspicion has focused on paraquat, a common herbicide with chemical structure similar to 1-methyl-4-phenylpyridinium (MPP+), the MPTP metabolite responsible for its neurotoxicity. Although in vitro evidence for paraquat neurotoxicity to dopamine cells is well established, its in vivo effects have been ambiguous because paraquat is di-cationic in plasma, which raises questions about its ability to cross the blood brain barrier. This study assessed the brain uptake of [11C]-paraquat in adult male rhesus macaques using quantitative PET imaging. Results showed minimal uptake of [11C]-paraquat in the macaque brain. The highest concentrations of paraquat were seen in the pineal gland and the lateral ventricles. Global brain concentrations including those in known dopamine areas were consistent with the blood volume in those structures. This acute exposure study found that paraquat is excluded from the brain by the blood brain barrier and thus does not readily support the causative role of paraquat exposure in idiopathic Parkinson''s disease. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
- View/download PDF
35. HPMA-based star polymer biomaterials with tuneable structure and biodegradability tailored for advanced drug delivery to solid tumours.
- Author
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Kostka, Libor, Kotrchová, Lenka, Šubr, Vladimír, Libánská, Alena, Ferreira, Carolina A., Malátová, Iva, Lee, Hye Jin, Barnhart, Todd E., Engle, Jonathan W., Cai, Weibo, Šírová, Milada, and Etrych, Tomáš
- Subjects
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STAR-branched polymers , *MOLECULAR weights , *ACRYLAMIDE , *BIOMATERIALS , *PROPIONIC acid , *TUMORS , *COPOLYMERS - Abstract
Design, controlled synthesis, physico-chemical and biological characteristics of novel well-defined biodegradable star-shaped copolymers intended for advanced drug delivery is described. These new biocompatible star copolymers were synthesised by grafting monodispersed semitelechelic linear (sL) N -(2-hydroxypropyl)methacrylamide copolymers onto a 2,2-bis(hydroxymethyl)propionic acid (bisMPA)-based polyester dendritic core of various structures. The hydrodynamic diameter of the star copolymer biomaterials can be tuned from 13 to 31 nm and could be adjusted to a given purpose by proper selection of the bisMPA dendritic core type and generation and by considering the sL copolymer molecular weight and polymer-to-core molar ratio. The hydrolytic degradation was proved for both the star copolymers containing either dendron or dendrimer core, showing the spontaneous hydrolysis in duration of few weeks. Finally, it was shown that the therapy with the biodegradable star conjugate with attached doxorubicin strongly suppresses the tumour growth in mice and is fully curative in most of the treated animals at dose corresponding approximately to one fourth of maximum tolerated dose (MTD) value. Both new biodegradable systems show superior efficacy and tumour accumulation over the first generation of star copolymers containing non-degradable PAMAM core. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
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