37 results on '"Buentello A"'
Search Results
2. Effective antimicrobial activity of ZnO and Yb-doped ZnO nanoparticles against Staphylococcus aureus and Escherichia coli
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Navarro-López, Diego Eloyr, Garcia-Varela, Rebeca, Ceballos-Sanchez, O., Sanchez-Martinez, A., Sanchez-Ante, Gildardo, Corona-Romero, Kaled, Buentello-Montoya, D.A., Elías-Zuñiga, Alex, and López-Mena, Edgar R.
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- 2021
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3. Design of a protocol for obtaining genomic DNA from saliva using mouthwash: Samples taken from patients with periodontal disease
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Mendoza, Ángel Chávez, Volante, Beatriz Buentello, Hernández, María Esther Ocharán, Mendoza, Claudia Camelia Calzada, Pliego, Arturo Flores, Baptista Gonzalez, Héctor A., and Juárez, Higinio Estrada
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- 2016
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4. A preliminary study of digestive enzyme activities and amino acid composition of early juvenile yellowfin tuna ( Thunnus albacares)
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Buentello, J. Alejandro, Pohlenz, Camilo, Margulies, Daniel, Scholey, Vernon P., Wexler, Jeanne B., Tovar-Ramírez, Dariel, Neill, William H., Hinojosa-Baltazar, Patricia, and Gatlin, Delbert. M., III
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- 2011
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5. Effects of dietary prebiotics on growth performance, immune response and intestinal morphology of red drum ( Sciaenops ocellatus)
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Zhou, Qi-Cun, Buentello, J. Alejandro, and Gatlin, Delbert M., III
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- 2010
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6. Effects of dietary protein source and protein–lipid source interaction on channel catfish ( Ictalurus punctatus) egg biochemical composition, egg production and quality, and fry hatching percentage and performance
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Sink, Todd D., Lochmann, Rebecca T., Pohlenz, Camilo, Buentello, Alejandro, and Gatlin, Delbert, III
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- 2010
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7. Mixed-valence porphyrin π-cation radical derivatives: Electrochemical investigations
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Scheidt, W. Robert, Buentello, Kristin E., Ehlinger, Noelle, Cinquantini, Arnaldo, Fontani, Marco, and Laschi, Franco
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- 2008
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8. The use of gasification solid products as catalysts for tar reforming.
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Buentello-Montoya, D.A., Zhang, X., and Li, J.
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BIOMASS gasification , *TAR , *CATALYSTS , *ACTIVATED carbon , *SYNTHESIS gas , *CHEMICAL potential - Abstract
Abstract The presence of tars in syngas is a major technological constraint for upscaling biomass gasification to produce heat, power, and other value-added chemicals such as biofuels. At the same time, the solid remains from biomass gasification i.e. char and ashes, have capabilities to catalyse the reforming of gasification tars. This work presents a comprehensive analysis of the relevance of gasification chars and ashes as catalysts for tar reforming. A description of the solid products from biomass gasification, their formation, chemical characteristics and potential applications is given. Additionally, a review of the state of the art of the uses of regular char, activated carbon and ashes as a catalyst for tar reforming is presented. Further, kinetics reported in literature, and the homogeneous and heterogeneous mechanisms for tar reforming over char are discussed and explained. From reviewing literature it was found that activated chars exhibit the best reforming capabilities, followed by regular char and ashes. Knowing the role of the interactions between the char and the tars is a key factor for optimization of char catalysts. Ultimately, this work provides guidance for understanding the uses of biomass solids as catalysts for tar reforming, and aid in future research to increase the economic feasibility of biomass gasification. Highlights • Catalysts from gasification solids comprise an opportunity for syngas tar removal. • Tar degradation over char occurs by coke deposition or reforming in the surface. • Ashes as catalysts are overlooked and provide good support for tar reforming. • Char catalytic activity can be sustained under adequate conditions. • Understanding the gas-solid interactions is key to optimizing catalyst performance. [ABSTRACT FROM AUTHOR]
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- 2019
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9. Investigation of competitive tar reforming using activated char as catalyst.
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Buentello-Montoya, David, Zhang, Xiaolei, Marques, Simão, and Geron, Marco
- Abstract
Abstract Syngas tar removal is one of the biggest challenges for the gasification of biomass as a clean energy source. Efforts to understand the reforming mechanism of tar compounds have been continuous during the last decades. Kinetic studies often employ a single tar species, neglecting possible interactions between different tar compounds. On the other hand, char, a by-product from biomass gasification, presents opportunities to catalyze tar reforming. In this work, reforming experiments were conducted in a fixed-bed reactor using syngas with a known mixture of benzene (C 6 H 6), toluene (C 7 H 8) and naphthalene (C 10 H 8). Hardwood char and an in-situ CO 2 -activated hardwood char were used as catalysts. The activated hardwood char exhibited the best reforming capabilities by converting 44% and 90% of the tars at 750 and 850°C, respectively, compared to 24% and 87% tar conversion obtained with the regular hardwood char at 750 and 850°C, respectively. From the experiments, a reduced mechanism model was proposed. This mechanism was used in computational simulations for analysis of the reaction kinetics, including possible catalyst selectivity. It was found that under a range of conditions, the benzene degradation is slower than its formation rate from toluene and naphthalene decomposition. This leads to increases in the benzene fraction at temperatures around 700°C. If the naphthalene and toluene concentrations are sufficient, benzene will accumulate even at high temperatures (around 800°C) regardless of residence times. It can be concluded that when benzene, toluene and naphthalene are present together, char favors the heterogeneous reforming of toluene and naphthalene, with benzene following an homogeneous reforming pathway. [ABSTRACT FROM AUTHOR]
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- 2019
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10. Immunohistochemical study of Metallothionein in patients with temporal lobe epilepsy.
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Juárez-Rebollar, Daniel, Alonso-Vanegas, Mario, Nava-Ruíz, Concepción, Buentello-García, Masao, Yescas-Gómez, Petra, Díaz-Ruíz, Araceli, Rios, Camilo, and Méndez-Armenta, Marisela
- Abstract
Epilepsy is characterized by spontaneous recurrent seizures and temporal lobe epilepsy (TLE) is the most common serious neurological example of acquired and frequent epilepsy. Oxidative stress is recognized as playing a contributing role in several neurological disorders, and most recently have been implicated in acquired epilepsies. The MTs occur in several brain regions and may serve as neuroprotective proteins against reactive oxygen species causing oxidative damage and stress. The main aim of this work was to describe the immunohistochemical localization of MT in the specimens derived from the patients affected by TLE. Histopathological examination showed NeuN, GFAP and MT immunopositive cells that were analyzed for determinate in hippocampal and parietal cortex samples. An increase in the reactive gliosis associated with increased MT expression was observed in patients with TLE. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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11. The effect of surface thermal radiation on entropy generation in an open cavity with natural convection.
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Hinojosa, J.F., Buentello, David, Xamán, J., and Pérez-Tello, Manuel
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NATURAL heat convection , *HEAT radiation & absorption , *ENTROPY , *FINITE volume method , *RAYLEIGH number - Abstract
In this work, a numerical study on entropy generation in a square open cavity with natural convection and surface thermal radiation is presented. The overall continuity, momentum, and energy equations for the gas phase in the open cavity were solved numerically by means of the finite-volume method. Temperature-dependent fluid properties were considered. During the calculations, the values of the Rayleigh number ( Ra ) were set in the range of 10 4 –10 6 . The temperature difference between the hot wall and the bulk fluid (ΔT) was varied between 50 and 400 K, and was represented by a dimensionless temperature difference (φ) for the purpose of generalization of the present study. The results of this investigation indicate that surface thermal radiation increases the overall entropy generation rate between 33.52% and 560.87%, and thus cannot be neglected in the analysis of this type of system. [ABSTRACT FROM AUTHOR]
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- 2017
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12. Co-gasification of waste PET, PP and biomass for energy recovery: A thermodynamic model to assess the produced syngas quality.
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Buentello-Montoya, D.A., Duarte-Ruiz, C.A., and Maldonado-Escalante, J.F.
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BIOMASS gasification , *BIOMASS energy , *SYNTHESIS gas , *POLYETHYLENE terephthalate , *PLASTIC scrap , *THERMODYNAMIC equilibrium - Abstract
Co-gasification of plastics with biomass is an attractive energy-recovery option to handle the plastic waste generated by society and requires further study. This work presents a simulation-based analysis of the air co-gasification of polypropylene (PP), polyethylene terephthalate (PET) and biomass (straw) using different combinations (PP/PET, PP/biomass, PET/biomass and PP/PET/biomass) and proportions, temperatures (650–850 °C), and equivalence ratios (0.25–0.45). A thermodynamic equilibrium model based on the Cantera chemistry toolbox and Python scripting was used in the simulations. Results indicate that increasing the proportion of plastics in the gasification feedstock increases the gas heating value (to a maximum of 5.78 MJ/Nm3) and tar contents (to a maximum of 72.89 g/Nm3). Additionally, it was found that PET is the plastic that adds the least value to the gas because of its lower heating value and tendency to form tar. Moreover, when gasifying a mixture of PP/PET/biomass, the gas H 2 /CO ratio decreases with temperature (from 1.91 at 650 °C to 1.14 at 750 °C and an equivalence ratio of 0.25), an aspect of particular importance for the end-use of the syngas. From the simulation results, a surrogate model was computed, and a series of response surface and polynomials were produced. [Display omitted] • Models to calculate Lower Heating Values and tar concentrations are presented. • PP provides better value than PET to the syngas from co-gasification. • Raising the temperature lowers the H 2 /CO ratio in plastic/biomass co-gasification. • In co-gasification of plastic/biomass, adding plastics increases the tar contents. [ABSTRACT FROM AUTHOR]
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- 2023
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13. 54. Long term surgical results in supplementary motor area epilepsy surgery
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Alonso-Vanegas, Mario A., Buentello Garcia, Ricardo M., Castillo-Montoya, Carlos, San-Juan, Daniel, Senties-Madrid, Horacio, Mascher, Erika Brust, Bialik, Paul Shkurovick, and Trenado, Carlos
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- 2016
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14. Toxicity of some essential oil formulations against the Mexican fruit fly Anastrepha ludens (Loew) (Diptera: Tephritidae).
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Buentello-Wong, S., Galán-Wong, L., Arévalo-Niño, K., Almaguer-Cantú, V., and Rojas-Verde, G.
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ESSENTIAL oils , *MEXICAN fruit fly , *ANASTREPHA , *EXTRACTION (Chemistry) , *CLOVE tree , *PHENYLPROPANOIDS - Abstract
Essential oils (EOs) extracted from Eugenia caryophyllus , Ocimum basilicum and Thymus vulgaris were evaluated for toxicity against adults of the fruit pest the Mexican fruit fly, Anastrepha ludens (Diptera: Tephritidae). The chemical composition of the EOs was also determined. The EOs of E. caryophyllus and O. basilicum contained primarily phenylpropanoids (77.58% and 72.63%, respectively), which were followed by benzoate esters (10.99%) and sesquiterpenes (6.22%) in E. caryophyllus and monoterpenes (16.65%) in O. basilicum . The EO of T. vulgaris was composed primarily of monoterpene hydrocarbons (89.39%). In ingestion toxicity assays, the EO of E. caryophyllus was the most toxic, with an LC50 of 3529 ppm, followed by the EOs of T. vulgaris and O. basilicum with LC50s of 5347 and 8050 ppm, respectively. At the highest concentration (1.5, 2.0 or 3.5% w/v), 100% mortality was observed with the three EOs in the ingestion toxicity assays. In general, the three EOs were significantly toxic to adult A. ludens . The development of a technology to incorporate the EOs into food bait could provide an alternative method to attract and kill A. ludens in field applications. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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15. Characterization of pantothenic acid deficiency and the dietary requirement of juvenile hybrid striped bass, Morone chrysops × M. saxatilis.
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Raggi, Thiago, Buentello, Alejandro, and Gatlin, Delbert M.
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STRIPED bass , *PANTOTHENIC acid , *NUTRITIONAL requirements , *WHITE bass , *DIETARY supplements - Abstract
An 8-week feeding trial was conducted to characterize the pantothenic acid deficiency signs and quantify the dietary requirement of juvenile hybrid striped bass, Morone chrysops × Morone saxatilis . A basal diet composed of fish muscle and crystalline amino acids was analyzed to contain 5 mg of pantothenate/kg and supplemented with calcium d -pantothenate resulting in five diets with graded pantothenate levels of 5, 10, 20, 30 and 40 mg/kg diet. Each diet was fed to triplicate groups of hybrid striped bass juveniles (initial average weight 1.6 g) in a recirculating system at 26.0 ± 1 °C. Fish fed the unsupplemented basal diet performed poorly in terms of growth parameters and exhibited typical signs of pantothenic acid deficiency such as hemorrhages, sluggishness, high mortality, anemia and severe hyperplasia of the epithelial cells of gill lamellae. Fish fed the diet containing 10 mg of pantothenate/kg diet performed significantly better than fish fed the basal diet but poorly when compared with the responses of fish fed diets containing 20, 30 and 40 mg of calcium d -pantothenate/kg diet. Also, pantothenic acid-deficiency signs were apparent in fish fed the diet containing 10 mg/kg diet; whereas, no deficiency signs were observed in fish fed diets supplemented with the higher levels. Based on these data, the dietary pantothenic acid requirement of hybrid striped bass was quantified at 18.8 mg/kg diet based on broken-line regression analysis of weight gain data. Statement of relevance Pantothenic acid deficiency in hybrid striped bass was characterized and the minimum dietary pantothenic acid requirement was determined to be 18.8 mg/kg based on weight gain. This information will assist in refining diet formulations for hybrid striped bass. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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16. Dietary arginine requirement of juvenile red drum (Sciaenops ocellatus) based on weight gain and feed efficiency.
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Barziza, Daniel E., Buentello, J. Alejandro, Barziza, D E, Buentello, J A, and Gatlin, D M 3rd
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RED drum (Fish) , *ARGININE , *ANIMAL nutrition , *FEED utilization efficiency , *AMINO acid metabolism , *ASPARTIC acid metabolism , *FISH physiology , *GLYCINE metabolism , *GLUTAMIC acid metabolism , *AGRICULTURE , *ANIMAL experimentation , *COMPARATIVE studies , *FOOD , *INGESTION , *RESEARCH methodology , *MEDICAL cooperation , *NUTRITIONAL requirements , *RESEARCH , *WEIGHT gain , *EVALUATION research - Abstract
Increasing aquacultural production of red drum (Sciaenops ocellatus) has prompted the determination of many of this species' nutritional requirements. However, limited information is available concerning its amino acid requirements, especially for arginine. Therefore, a feeding trial was conducted with juvenile red drum to determine their quantitative dietary requirement for arginine. Experimental diets contained 35 g crude protein/100 g from red drum muscle and crystalline amino acids. Incremental levels of arginine were added to the diets in place of a mixture of glycine and aspartic acid to maintain all diets isonitrogenous. All diets were fed in triplicate to juvenile red drum for 7 wk. Graded levels of arginine significantly (P < 0.05) affected weight gain, feed efficiency, protein efficiency ratio (PER) and plasma arginine levels of the fish. Based on least-squares regression of feed efficiency and PER data, the minimum requirement (+/- SEM) of red drum for arginine was estimated at 1.44 (+/- 0.15) and 1.48 (+/- 0.12) g/100 g diet (4.11 and 4.23 g/100 g dietary protein), respectively. The arginine requirements estimated from weight gain data were 1.75 (+/- 0.18) g/100 g diet or 5.0 g/100 g dietary protein. These values are similar to those reported for other carnivorous fish species. [ABSTRACT FROM AUTHOR]
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- 2000
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17. Minimizing fishmeal use in juvenile California yellowtail, Seriola lalandi, diets using non-GM soybeans selectively bred for aquafeeds.
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Buentello, Alejandro, Jirsa, Dave, Barrows, Frederic T., and Drawbridge, Mark
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FISH meal , *GENETICALLY modified foods , *SOYBEAN , *LOW-protein diet , *CALIFORNIA yellowtail , *CULTIVARS , *OLIGOSACCHARIDES - Abstract
A study was undertaken to examine the potential of two non-genetically modified (non-GM) soy products, differentially processed, as fishmeal (FM) replacements. Dietary protein (DP) was replaced at 50, 60 and 70% using the 3011 meal or 40, 50 and 60% with the 3032 meal. Ingredient 3011 was a cooked and solvent-extracted meal from a high protein and low oligosaccharide soy cultivar; whereas, ingredient 3032 was a cold-pressed, low fat cake ground to a meal that originated from a medium protein, low oligosaccharide, low protease inhibitor soy cultivar. Experimental diets were isonitrogenous (45% crude protein, CP), isolipidic (13% lipid), isocaloric (12.6 kJ energy kg − 1 of diet) and were balanced for lysine (3.2%) and methionine + cysteine (2%). Taurine was supplemented at 1.5% in all diets. A commercial control diet (52% CP and 13% lipid) was included for a total of eight test diets. Twenty four round tanks (330 L) were each stocked with 20, 4 g yellowtail ( Seriola lalandi ). Tanks were supplied with recirculated seawater and each diet was randomly assigned to three replicate tanks. After a 10-week feeding trial, growth, survival, feed conversion ratio, protein efficiency ratio, protein retention, condition factor and carcass proximate composition were contrasted among dietary treatments. Results indicate that juvenile yellowtail fed diets in which the 3032 meal provided 50 and 60% of DP grew significantly ( P < 0.001) better than those fed all other diets, including the FM-based (menhaden FM) and commercial control diets. Fish fed with the 3011 meal providing 50% of DP and those fed with the 3032 meal providing 40% of DP had similar growth performance which was slightly greater than the fish fed the FM reference diet. Minor intestinal histology differences were found among fish fed the 8 diets. Taken together these data indicates that the improved non-GM soybean meals supported better performance of juvenile yellowtail than fish fed the FM reference diet. No enteritis was detected even at high dietary inclusion levels. [ABSTRACT FROM AUTHOR]
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- 2015
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18. Partial replacement of fishmeal, poultry by-product meal and soy protein concentrate with two non-genetically modified soybean cultivars in diets for juvenile cobia, Rachycentron canadum.
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Watson, Aaron M., Buentello, Alejandro, and Place, Allen R.
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SOY proteins , *SOYBEAN varieties , *FISH feeds , *COBIA , *FISH growth , *PLANT proteins , *AQUACULTURE - Abstract
Fishmeal (FM) replacement in diets for intensive aquaculture has become a high priority area for the global aquaculture industry. In this study, a twelve week growth trial was conducted with juvenile cobia (18 g initial weight) to examine the effects of non-genetically modified soybean meals as potential replacement protein sources. Genetically modified (GM) crops and their intended and unintended effects have become major topics of controversy worldwide, with several regions banning their use in food and feeds. Therefore, it is especially critical to develop and evaluate non-GM feedstuffs for use in aquaculture diets where GM products are prohibited as the global aquaculture industry continues its expansion to meet increasing demands. Navita Premium Feed Ingredients (NPFI's) 3010 solvent extracted meal and 3032 cold-pressed cake meal were utilized to replace 50, 60 or 70% and 40, 50, or 60% of FM protein, respectively and were compared to a FM based reference formulation. None of the experimental diets performed significantly different from the reference diet in terms of weight gain (WG) or specific growth rate (SGR). However the 3010 50% diet performed significantly better than the 3032 50 and 60% diets in regard to WG, SGR, and protein efficiency. There were no significant differences between the seven diets at the conclusion of the trial in regard to their effects on filet quality as determined by organoleptic testing. These two non-GM soybean protein sources appear to be valuable FM replacement options for juvenile cobia, with none of the typical indicators of intestinal enteritis developing as has been observed in various other teleost species when high quantities of commodity soybean meal have been utilized. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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19. Homozygosity mapping identifies a GALK1 mutation as the cause of autosomal recessive congenital cataracts in 4 adult siblings.
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Chacon-Camacho, Oscar F., Buentello-Volante, Beatriz, Velázquez-Montoya, Roberto, Ayala-Ramirez, Raul, and Zenteno, Juan C.
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HOMOZYGOSITY , *GENE mapping , *GENETIC mutation , *CONGENITAL heart disease in adolescence , *MONOGENIC functions , *OPHTHALMIC surgery complications - Abstract
Abstract: Objective: Monogenic congenital cataract is one of the most genetically heterogeneous ocular conditions with almost 30 different genes involved in its etiology. In adult patients, genotype–phenotype correlations are troubled by eye surgery during infancy and/or long-term ocular complications. Here, we describe the molecular diagnosis of GALK1 deficiency as the cause of autosomal recessive congenital cataract in a family from Costa Rica. Methods: Four affected siblings were included in the study. All of them underwent eye surgery during the first decade but medical records were not available. Congenital cataract was diagnosed by report. Molecular analysis included genome wide homozygosity mapping using a 250K SNP Affymetrix microarray followed by PCR amplification and direct nucleotide sequencing of candidate gene. Results: Genome wide homozygosity mapping revealed a 6Mb region of homozygosity shared by two affected siblings at 17q25. The GALK1 gene was included in this interval and direct sequencing of this gene revealed a homozygous c.1144C>T mutation (p.Q382*) in all four affected subjects. Conclusions: This work demonstrates the utility of homozygosity mapping in the retrospective diagnosis of a family with congenital cataracts in which ocular surgery at early age, the lack of medical records, and the presence of long term eye complications, impeded a clear clinical diagnosis during the initial phases of evaluation. [Copyright &y& Elsevier]
- Published
- 2014
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20. Beyond harm-producing versus harm-reducing: A qualitative meta-synthesis of people who use drugs' perspectives of and experiences with the extramedical use and diversion of buprenorphine.
- Author
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Sud, Abhimanyu, Salamanca-Buentello, Fabio, Buchman, Daniel Z., Sabioni, Pamela, and Majid, Umair
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BUPRENORPHINE , *OPIOID abuse , *GROUNDED theory - Abstract
Introduction: This review synthesizes the literature on the perspectives and experiences of people who use drugs to better understand motivations and behaviors related to the extramedical use and diversion of buprenorphine. Given the particular social construction of buprenorphine against methadone, and the centrality of concerns around extramedical use in delivering opioid agonist therapies, a focus on extramedical buprenorphine use can provide an important lens through which to analyze treatment for opioid use disorder. This review is framed within persistent tensions between potential harm-producing versus harm-reducing effects of extramedical use that have long been described for opioid agonist therapies.Methods: The research team conducted a qualitative meta-synthesis based on a systematic search of eight databases as well as hand searching. The review includes all primary qualitative and mixed-methods studies related to the perspectives and experiences of people who use drugs on extramedical buprenorphine use. The study team carried out three rounds of qualitative coding using NVivo 12, and constructivist grounded theory and the constant comparative method informed the synthesis.Results: The review includes twenty-one studies. Findings are organized into the following three themes: 1) the experiences of people who use drugs (PWUD) with extramedical use of buprenorphine and their motivations to engage in it (including the desire to self-medicate and achieve "stability", to manage ongoing use of other opioids, and to "get high"); 2) the relationship between extramedical use and formal medical opioid agonist therapy programs; and 3) the established drug economy of extramedical buprenorphine.Conclusions: The review identified varied and often divergent perspectives and experiences with extramedical buprenorphine use. An examination of the reported "normalizing" effects of extramedical buprenorphine suggests this practice as extending medicalized discipline beyond the clinical environment. Taken together, these findings identify a need to move beyond the tension of harm-reducing versus harm-producing effects toward forms of health care and promotion that focus on the needs, perspectives, and priorities of people who use drugs. [ABSTRACT FROM AUTHOR]- Published
- 2022
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21. Effects of dietary arginine on endocrine growth factors of channel catfish, Ictalurus punctatus.
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Pohlenz, Camilo, Buentello, Alejandro, Miller, Thomas, Small, Brian C., MacKenzie, Duncan S., and Gatlin, Delbert M.
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ARGININE , *ENDOCRINE gland physiology , *CHANNEL catfish , *FISH nutrition , *FISH growth , *SOMATOMEDIN C - Abstract
Abstract: Thyroid (TH) and growth (GH) hormones, and insulin-like growth factor I (IGF-I) are anabolic regulators in fish and responsive to nutrient intake. A study was conducted to determine if previously reported growth effects of dietary arginine (ARG) in channel catfish were related to the activation of endocrine axes. In a first experiment, catfish were fed incremental levels of ARG (0.5 – 4% of diet) for 6weeks and sampled at 2-week intervals. In a second experiment, fasted (48h) fish were fed a single ration of ARG (0.5 or 4% of diet) and sampled at various intervals (0 to 72h postprandial, PP). Experiment 1 did not reveal any influence of ARG on circulating TH, GH, or IGF-I despite the significantly increased growth of fish fed ARG-enriched diets. In experiment 2, feeding the 4% ARG diet significantly increased the amplitude of pulsatile plasma GH levels and also significantly increased IGF-I mRNA in liver and muscle, (at 2h PP) and plasma IGF-I levels (at 6h PP). Although relatively infrequent sampling failed to reveal alterations in TH or GH levels in response to ARG-induced growth activation, PP high frequency sampling unveiled high amplitude pulsatile GH secretions and may be important in activating IGF production in target tissues. Additionally, expressed and secreted IGF-I exhibited discernible patterns which closely correlate with ARG-induced growth effects in catfish. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
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22. Free dietary glutamine improves intestinal morphology and increases enterocyte migration rates, but has limited effects on plasma amino acid profile and growth performance of channel catfish Ictalurus punctatus
- Author
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Pohlenz, Camilo, Buentello, Alejandro, Bakke, Anne Marie, and Gatlin, Delbert M.
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CHANNEL catfish , *GLUTAMINE , *ENTEROCYTES , *PLASMA amino acids , *FISH growth , *FISH feeds , *GELATIN , *CASEINS - Abstract
Abstract: The present experiment was conducted to investigate the effects of graded amounts of dietary GLN on the intestinal morphology, plasma amino acids and growth performance of juvenile channel catfish. Six experimental diets (280gkg−1 crude protein, 80gkg−1 lipid) were composed of casein, gelatin and crystalline amino acids and supplemented with 0, 5, 10, 15, 20 or 30g free glutamine kg−1. These diets were fed to triplicate groups of fish (average initial weight of 6.1) for 10weeks after which blood and intestinal samples were collected. Intestinal microstructures were positively affected by dietary GLN at the highest levels of supplementation (20 and 30gkg−1), significantly (P <0.05) increasing enterocyte and microvilli height in anterior, mid and posterior intestinal sections. Moreover, a subsequent experiment revealed that GLN supplementation significantly (P <0.05) increased the enterocyte migration rate in all sampled intestinal segments. In contrast, immunohistochemical staining of proliferating cells with mouse anti-proliferating cell nuclear antigen (PCNA), revealed an increased number of proliferating cells only at the distal intestine. Plasma amino acid levels 15h postprandial showed significantly (P <0.05) higher levels of asparagine, serine, glycine and threonine in fish fed the diet supplemented with 20g GLN kg−1 diet. Despite the consistent trend of higher values seen in fish fed the diet supplemented with 20g GLN kg−1, there were no significant (P >0.05) differences in growth-related parameters among treatments. The present results demonstrate an efficient utilization of free GLN by intestinal mucosal cells of channel catfish, which resulted in enhancement of the enteric microstructure along with increased migration rates of enterocytes and modified plasma amino acid profiles. However, these results were not translated into improved growth at the end of the 10-week feeding trial. [Copyright &y& Elsevier]
- Published
- 2012
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23. Effects of dietary arginine and glutamine on growth performance, immune responses and intestinal structure of red drum, Sciaenops ocellatus
- Author
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Cheng, Zhenyan, Buentello, Alejandro, and Gatlin, Delbert M.
- Subjects
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ARGININE , *GLUTAMINE , *IMMUNE response , *RED drum (Fish) , *FISH immunology , *FISH growth , *COLLAGEN , *LYSOZYMES - Abstract
Abstract: Both arginine and glutamine play important roles in tissue repair, cell replication and collagen synthesis which impact animal growth and survival. Thus, a 7-week feeding trial was conducted to determine the effects of dietary arginine and glutamine on growth performance, innate immune responses and intestinal structure of juvenile red drum, Sciaenops ocellatus (6.9±0.04g, initial average weight). Protein in all experimental diets was derived from menhaden fishmeal and dehulled, solvent-extracted soybean meal to provide isonitrogenous (44% crude protein) and isolipidic (10%) diets. The basal diet contained 0.14% glutamine and 1.72% arginine on a dry-matter basis, which exceeds the established dietary requirement of red drum. Experimental diets were prepared by supplementing the basal diet with either arginine at 1% of dry weight, glutamine at 1%, glutamine at 2%, or both arginine and glutamine at 1%, with adjustments in glycine to maintain equal nitrogen among all diets. Feed efficiency was significantly (P <0.05) improved by supplementation of glutamine at 2% and the combination of both arginine and glutamine at 1% of diet. Neutrophil oxidative radical production in fish fed the glutamine and/or arginine-supplemented diets was significantly (P =0.03) higher compared with that of fish fed the basal diet, with a synergistic effect observed in fish fed the combined arginine–glutamine diet. Significantly (P <0.05) higher serum lysozyme activity also was observed in fish fed the diet supplemented with 1% of both arginine and glutamine. Extracellular superoxide anion production by red drum macrophages was significantly (P <0.05) higher for fish fed diets with glutamine at either 1 or 2% and the 1% arginine–glutamine combination compared to fish fed the basal diet. Similarly, macrophages from fish fed the diet with glutamine at 2% and the arginine–glutamine combination at 1% produced significantly higher amounts of intracellular superoxide anion. Morphometric analyses – i.e., measurements of enterocyte, microvillus and fold heights – demonstrated positive effects of both dietary glutamine and arginine in different portions (proximal, mid and distal) of the gastrointestinal tract of red drum. In general, fish fed the diet supplemented with 2% glutamine had the greatest increases in magnitude of the chosen structures, although those fed the diet with arginine at 1% also yielded improved scores for some enteric portions. Therefore, results from the present study establish the importance of both dietary arginine and glutamine supplementation in improving feed efficiency, as well as eliciting positive changes to several components of the innate immune system and intestinal functionality of red drum. [Copyright &y& Elsevier]
- Published
- 2011
- Full Text
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24. The role of reality enhancing technologies in teaching and learning of mathematics.
- Author
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Buentello-Montoya, D.A., Lomelí-Plascencia, M.G., and Medina-Herrera, L.M.
- Subjects
- *
COMPUTER literacy , *AUGMENTED reality , *VIRTUAL reality , *MATHEMATICS , *DIGITAL natives ,DEVELOPING countries - Abstract
• AR and VR have been successfully used to improve the student's performance. • A challenge for AR and VR lies in the digital literacy of teachers and students. • AR and VR require user's prior preparation to be exploited at its fullest. • The use of AR and VR in education might globally increase over the coming years. Virtual and Augmented Reality (VR and AR, respectively) are technologies with potential impact in education, and can facilitate subjects, such as mathematics, that may be difficult for students. This manuscript presents a comprehensive review of works using VR and/or AR for teaching mathematics at all academic levels during the timespan from 2015 to June 2020. The work contributes by providing an overview of the findings, challenges, and trends for the use of these technologies. Results from the review indicate that both VR and AR have seen acceptance by students but using the technologies might be complicated if lectures are not designed adequately. Moreover, the number of publications involving AR and VR in teaching and learning maths has increased over time, and the demographics indicate that the technology is taking prevalence in developing countries. [Display omitted] [ABSTRACT FROM AUTHOR]
- Published
- 2021
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25. An overview of Neem (Azadirachta indica) and its potential impact on health.
- Author
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Islas, Jose Francisco, Acosta, Ezeiza, G-Buentello, Zuca, Delgado-Gallegos, Juan Luis, Moreno-Treviño, María Guadalupe, Escalante, Bruno, and Moreno-Cuevas, Jorge E.
- Abstract
• Global health benefits form from plant-based research. • Insights into the molecular compounds found in Azadirachta indica. • Health benefits derived from Azadirachta indica extracts. • Industrial and other modern applications of Azadirachta indica. Global health and medical practice seek to merge alternative medicine with evidence-based medicine for a better understanding of the metabolic process and its effects in the human body. An example is the use of complementary medicine like phytotherapy. Azadirachta indica (Neem), a tree originally from India and Myanmar, called by many "The village pharmacy" or "Divine tree" because of its many health properties. In recent times, Neem-derived extracts have been shown to work from anywhere from insect repellent, to supplements to lower inflammation, diabetic control, and even to combat cancer. Herein, we state the health benefits found in diverse compounds and extracts derived from Neem, highlighting the mechanisms and pathways in which Neem compounds produce their effects, while warning that the improper and unstandardized conditions to produce extracts can lead to health issues, particularly certain compounds might have damaging effects on the liver and kidneys. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
26. Performance of biochar as a catalyst for tar steam reforming: Effect of the porous structure.
- Author
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Buentello-Montoya, David, Zhang, Xiaolei, Li, Jun, Ranade, Vivek, Marques, Simão, and Geron, Marco
- Subjects
- *
BIOMASS gasification , *STEAM reforming , *TAR , *CARBON offsetting , *BIOCHAR , *CATALYSTS , *SCANNING electron microscopy - Abstract
• The catalytic performance of char can be maintained at high temperatures (e.g. 850 °C). • Microporous chars deactivate faster than mesoporous chars. • At elevated temperatures activated char and regular char demonstrate similar performance. • Simulations showed that mesoporous chars conserve their catalytic activity for over 10 h. • Coke in micropores cannot be gasified even at high temperatures (e.g. 850 °C). The application of gasification to thermally treat biomass as carbon neutral resources has been constrained by the technical challenges associated with tar formations, which cause operational problems in downstream equipment for syngas processing. Catalysts, such as transition metals, calcined rocks and char, can be used to catalyse tar reforming. Biochars, which are naturally produced during biomass gasification, are particularly attractive as an alternative catalyst due to their catalytic functions, low cost and long endurance. Despite these promising characteristics, adequate knowledge on the relationship between the porous structure of biochar and its deactivation by coking during the steam reforming of tars is not available. In this work, the influence of the porous structure of biochar on its performance across time for reforming tar was investigated in a fixed-bed reactor, over a temperature range from 650 to 850 °C. Regular biochar and physically activated biochar from the same precursor biomass were employed as bed material. The tar samples were the composed mixture of benzene, toluene and naphthalene. Both fresh and spent catalysts were analysed with Brunauer-Emmet-Teller, t-plot, Fourier Transform Infrared and Scanning Electron Microscopy/Energy Dispersive Spectroscopy. Results showed that, while at moderate temperatures of 650 and 750 °C, the activated biochar offered a higher tar conversion but more severe deactivation than that of the regular biochar. At the high temperature of 850 °C, the difference in the catalytic performance between the two chars was negligible, and over 90% of the initial tar species were removed throughout the 3-hour long experiments. At 850 °C, the coke deposited in the meso- and macro-pores of both chars was gasified, leading to a stable catalytic performance of both chars. The results indicated that meso- and macro-porous biochars are resilient and active enough to become a viable option for tar steam reforming. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
27. Evaluation of new non-genetically modified soybean varieties as ingredients in practical diets for Litopenaeus vannamei.
- Author
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Fang, Xiaoyun, Yu, Denghang, Buentello, Alejandro, Zeng, Peng, and Davis, D. Allen
- Subjects
- *
SOYBEAN varieties , *WHITELEG shrimp , *DIETARY supplements , *SOYBEAN meal , *PRINCIPAL components analysis - Abstract
Twelve ingredients obtained from new, non-genetically modified soybean cultivars as well as commercially available sources of soybean meal (SBM) were evaluated in practical feed formulations for the Pacific white shrimp, Litopenaeus vannamei , using both digestibility and growth trials. Apparent digestibility coefficients for energy (ADE) and protein (ADP) were determined using chromic oxide as inert marker and the 70:30 replacement technique. Digestibility coefficients for ADE and ADP ranged from 76.6 to 91.3% and 93.6 to 99.8%, respectively. In general, two distinct groups of high and moderate digestibility values were observed. A separate 8-week growth trial was conducted to evaluate selected SBMs using six replicate tanks per dietary treatment (10 shrimp per tank, initial weight 0.52 ± 0.04 g). An SBM-based reference diet was formulated using commercial SBM (45.3), which was then completely replaced on iso-nitrogenous basis with other experimental SBMs. Significant differences ( P ≤ 0.05) were observed in growth performance but not survival. In general, trends in digestibility values were similar to those of the growth trial. Ingredients (I)-16, I-17, I-18, and I-19 demonstrate relatively higher values in terms of digestibility as well as supporting good growth. Results indicate that genetic selection of soybean for nutritional characteristics of the meal has the potential to increase the nutritional values of SBM for shrimp feeds. Statement of relevance This study provides useful and important information of 12 varieties soybean meal in shrimp to support the aquaculture feed industry development. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
28. Effect of catalytic hydrodynamics over microagglomerates of Mn2O3 and PdO supported on γ-χ-Al2O3 for dimethyl ether production.
- Author
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Armenta, M.A., Maytorena, V.M., Buentello-Montoya, D.A., Arroyo, E., Cota-Leal, M., Yong, D., and Olivas, A.
- Subjects
- *
CATALYSIS , *METHYL ether , *PALLADIUM oxides , *HYDRODYNAMICS , *MANGANESE oxides , *HETEROGENEOUS catalysts - Published
- 2022
- Full Text
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29. Exome sequencing identifies RDH12 compound heterozygous mutations in a family with severe retinitis pigmentosa.
- Author
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Chacon-Camacho, Oscar F., Jitskii, Serguei, Buentello-Volante, Beatriz, Quevedo-Martinez, Jonathan, and Zenteno, Juan C.
- Subjects
- *
NUCLEOTIDE sequence , *RETINOL dehydrogenase , *GENETIC mutation , *RETINITIS pigmentosa , *RETINAL degeneration , *VISION disorders , *GENE expression , *GENETICS - Abstract
Abstract: Objective: Retinitis pigmentosa (RP) is the most prevalent type of inherited retinal degeneration and one of the commonest causes of genetically determined visual dysfunction worldwide. To date, approximately 35 genes have been associated with nonsyndromic autosomal recessive RP (arRP), however the small contribution of each gene to the total prevalence of arRP and the lack of a clear genotype–phenotype correlation complicate the genetic analysis in affected patients. Next generation sequencing technologies are powerful and cost-effective methods for detecting causative mutations in both sporadic and familial RP cases. Methods: A Mexican family with 5 members affected from arRP was studied. All patients underwent a complete ophthalmologic examination. Molecular methods included genome-wide SNP homozygosity mapping, exome sequencing analysis, and Sanger-sequencing confirmation of causal mutations. Results: No regions of shared homozygosity among affected subjects were identified. Exome sequencing in a single patient allowed the detection of two missense mutations in the RDH12 gene: a c.446T>C transition predicting a novel p.L149P substitution, and a c.295C>A transversion predicting a previously reported p.L99I replacement. Sanger sequencing confirmed that all affected subjects carried both RDH12 mutations. Conclusions: This study adds to the molecular spectrum of RDH12-related retinopathy and offers an additional example of the power of exome sequencing in the diagnosis of recessively inherited retinal degenerations. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
30. Dietary supplementation of arginine and/or glutamine influences growth performance, immune responses and intestinal morphology of hybrid striped bass (Morone chrysops × Morone saxatilis)
- Author
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Cheng, Zhenyan, Gatlin, Delbert M., and Buentello, Alejandro
- Subjects
- *
DIETARY supplements , *ARGININE , *IMMUNE response , *WEIGHT gain , *STRIPED bass , *FISH growth , *FISH morphology , *ANIMAL species - Abstract
Abstract: Various studies with fish species indicate that dietary arginine (Arg) and glutamine (Gln) supplementation may support an enhanced performance beyond simply improving weight gain. Therefore, a feeding trial was conducted to determine the effects of dietary Arg or Gln on growth performance, immune responses and intestinal morphology of juvenile hybrid striped bass, Morone chrysops × Morone saxatilis (4.1±0.02g, initial average weight). The basal diet was formulated from menhaden fishmeal and solvent-extracted soybean meal to contain 45% crude protein and 10% lipid. Arginine and Gln were singularly added to the basal diet at either 1% or 2% or both amino acids were added in combination at 1% to formulate experimental diets with adjustments in glycine to maintain all diets iso-nitrogenous. Fish were fed the experimental diets for a period of 8weeks, at the end of which final weight, specific growth rate and feed efficiency were significantly (P <0.05) higher in fish fed diets supplemented with Arg at either 1 or 2%, Gln at 1%, Arg and Gln both at 1%, but not in those fed the diet supplemented with Gln at 2%. Arginine and/or Gln supplementation tended to improve neutrophil oxidative radical production, but not significantly (P >0.05). However, significantly higher serum lysozyme activity was observed in fish fed the diet supplemented with Arg at either 1 or 2% and Gln at 1%. Intracellular superoxide anion production by hybrid striped bass macrophages also tended to increase, while extracellular superoxide anion production was significantly improved by supplementation of Arg at 1% and Gln at either 1 or 2% of diet. Morphometric analyses demonstrated positive effects of both dietary Arg and Gln on histo-morphological measurements in different portions of the gastrointestinal tract of hybrid striped bass. The 1% Arg diet resulted in the greatest increases in size of the chosen enteric structures, but the other four experimental diets also significantly improved the scores for various intestinal portions compared to fish fed the basal diet. Results from the present experiment demonstrate the importance of dietary supplementation of both Arg and Gln in improving growth performance, eliciting positive changes to several components of the innate immune system, and also benefiting the intestinal functionality of hybrid striped bass. [Copyright &y& Elsevier]
- Published
- 2012
- Full Text
- View/download PDF
31. Highly dispersed Ag2O-CuO nanospheres supported on γ-χ-Al2O3 for methanol dehydration to dimethyl ether.
- Author
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Armenta, M.A., Alvarez, Luis H., Maytorena, V.M., Buentello-Montoya, D.A., Silva-Rodrigo, R., Olivas, A., and Flores-Sánchez, L.A.
- Subjects
- *
CHEMICAL systems , *METHYL ether , *NANOPARTICLE size , *DEHYDRATION , *CATALYTIC activity , *METHANOL - Abstract
[Display omitted] • Methanol dehydration using Ag 2 O-CuO/γ-χ-Al 2 O 3 as a catalyst. • Ag 2 O-CuO/γ-χ-Al 2 O 3 reached selectivity to DME of 100 %. • Effect of nanoparticle shape and size on methanol dehydration. In this manuscript, the wet impregnation method was used to support Ag 2 O, CuO, and Ag 2 O-CuO nanoparticles on γ-χ-Al 2 O 3 to assess their catalytic capabilities in the methanol dehydration (MD) reaction to produce dimethyl ether (DME); the operation conditions were 1 atm and temperature ranging between 200 and 240 °C. It was found that Ag 2 O-CuO/γ-χ-Al 2 O 3 catalyst result in MD conversions up to 88 % and selectivities of 100 %, under 240 °C conditions. The catalytic activity of the Ag 2 O-CuO/γ-χ-Al 2 O 3 catalyst is related to the high dispersion, homogeneity and larger size of the Ag 2 O-CuO nanoparticles (∼12.6 nm) compared to Ag 2 O and CuO nanoparticles, which have sizes of ∼ 9.52 and 10 nm, respectively. Additionally, the catalytic activity of the Ag 2 O-CuO/γ-χ-Al 2 O 3 material is related to its total acidity (weak and moderate), 0.056 mmol/g, showing a difference of 25 %, 28.57 and 5.35 % with respect to the γ-χ-Al 2 O 3 , CuO/γ-χ-Al 2 O 3 and Ag 2 O/γ-χ-Al 2 O 3 materials, respectively. Finally, the catalytic stability measurements of the Ag 2 O-CuO/γ-χ-Al 2 O 3 catalyst remained relatively constant over time, presenting a conversion value of 79 % after 2.6 h of reaction due to the low apparent activation energy, 112.6 kJ/mol. The results of the exergy analysis indicate that the chemical exergy of the system ranges between 1/6 and 1/3 of the physical exergy, and most of the energy available to produce work is in the form of heat. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
32. Increased expression of BDNF transcript with exon VI in hippocampi of patients with pharmaco-resistant temporal lobe epilepsy.
- Author
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Martínez-Levy, G.A., Rocha, L., Lubin, F.D., Alonso-Vanegas, M.A., Nani, A., Buentello-García, R.M., Pérez-Molina, R., Briones-Velasco, M., Recillas-Targa, F., Pérez-Molina, A., San-Juan, D., Cienfuegos, J., and Cruz-Fuentes, C.S.
- Subjects
- *
TEMPORAL lobe epilepsy , *BRAIN-derived neurotrophic factor , *GENE expression , *HIPPOCAMPUS (Brain) , *EXONS (Genetics) , *PROMOTERS (Genetics) , *GENETICS - Abstract
A putative role of the brain-derived neurotrophic factor (BDNF) in epilepsy has emerged from in vitro and animal models, but few studies have analyzed human samples. We assessed the BDNF expression of transcripts with exons I (BDNFI), II (BDNFII), IV (BDNFIV) and VI (BDNFVI) and methylation levels of promoters 4 and 6 in the hippocampi of patients with pharmaco-resistant temporal lobe epilepsy (TLE) ( n = 24). Hippocampal sclerosis (HS) and pre-surgical pharmacological treatment were considered as clinical independent variables. A statistical significant increase for the BDNFVI ( p < 0.05) was observed in TLE patients compared to the autopsy control group ( n = 8). BDNFVI was also increased in anxiety/depression TLE ( N = 4) when compared to autopsies or to the remaining group of patients ( p < 0.05). In contrast, the use of the antiepileptic drug Topiramate (TPM) ( N = 3) was associated to a decrease in BDNFVI expression ( p < 0.05) when compared to the remaining group of patients. Methylation levels at the BDNF promoters 4 and 6 were similar between TLE and autopsies and in relation to the use of either Sertraline (SRT) or TPM. These results suggest an up-regulated expression of a specific BDNF transcript in patients with TLE, an effect that seems to be dependent on the use of specific drugs. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
33. Expressed IgH μ and τ transcripts share diversity segment in ranched Thunnus orientalis.
- Author
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Mashoof, Sara, Pohlenz, Camilo, Chen, Patricia L., Deiss, Thaddeus C., Gatlin, Delbert, Buentello, Alejandro, and Criscitiello, Michael F.
- Subjects
- *
IMMUNOGLOBULINS , *GENETIC transcription , *TUNA , *GENE expression in fishes , *NUCLEOTIDE sequence , *POLYMERASE chain reaction - Abstract
Highlights: [•] Ig μ and τ were analyzed in the Pacific bluefin tuna, Thunnus orientalis. [•] Repertoire analysis shows one shared diversity (DH) segment by μ and τ. [•] The shared DH segment suggests a divergent isotype control mechanism. [•] qPCR shows a lower relative predominance of IgM in the gills to IgT. [•] Ig μ and τ CDR3 lengths are similar in tuna, perhaps owing to the shared DH. [Copyright &y& Elsevier]
- Published
- 2014
- Full Text
- View/download PDF
34. Replacement of fish meal by a novel non-GM variety of soybean meal in cobia, Rachycentron canadum: Ingredient nutrient digestibility and growth performance.
- Author
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Suarez, Jorge A., Tudela, Carlos, Davis, Drew, Daugherty, Zachary, Taynor, Matthew, Glass, Lindsay, Hoenig, Ronald, Buentello, Alejandro, and Benetti, Daniel D.
- Subjects
- *
FISH meal as feed , *SOYBEAN meal as feed , *COBIA , *PROTEIN content of fish as food , *AQUACULTURE , *FISH growth - Abstract
Abstract: A constraint for the expansion of cobia aquaculture is the availability of high quality formulated diets which reduce or eliminate fish meal (FM) protein. Therefore, the nutritive value of a novel soybean cultivar, Navita™ (Navita, non-genetically modified and selectively bred soy), and regular, commodity soybean meal (SBM, de-hulled, defatted, roasted and solvent-extracted) was evaluated for cobia, Rachycentron canadum via separate digestibility and growth trials. In the first experiment Navita's apparent digestibility coefficients (ADC) were higher than those of SBM for nearly every nutrient evaluated. Crude protein ADCs were 82 and 69% for Navita and SBM, respectively. Apparent DC for amino acids ranged from 68 to 109% for Navita whereas, amino acid ADCs for SBM varied from 42 to 98%. The feeding trial utilized fish of a size that more closely resembles commercial cobia stocking (1.8kg), and was conducted over a 91-day period. Experimental diets (iso-nitrogenous and iso-energetic) were formulated such that 67% of the FM protein in the reference diet was replaced by either a combination of SBM+soy protein concentrate (SPC, Solae Profine®) labeled MXSB-diet, or by a combination of SPC+Navita; Navita-diet, hereafter. A fourth experimental diet had 80% of the FM protein replaced by a combination of Navita+SPC and was identified as Navita-high. No significant differences (P >0.05) were observed in fish fed the experimental diets for feed conversion ratio, protein efficiency ratio, feed efficiency, mean daily intake, gross protein intake, gross energy intake, visceral somatic index, muscle ratio, and hepatosomatic index. Fish fed the Navita-high diet had the lowest fish in:fish out ratio (FIFO) at 0.9±0.16. These results indicate that Navita meal can be incorporated at very high levels in the diet of marine carnivorous fish such as cobia with no detriment to performance, making it a prime candidate for FM replacement in aquafeeds. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
35. The effect of NovaSil dietary supplementation on the growth and health performance of Nile tilapia (Oreochromis niloticus) fed aflatoxin-B1 contaminated feed
- Author
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Zychowski, Katherine E., Pohlenz, Camilo, Mays, Travis, Romoser, Amelia, Hume, Michael, Buentello, Alejandro, Gatlin, Delbert M., and Phillips, Timothy D.
- Subjects
- *
DIETARY supplements , *FISH growth , *NILE tilapia , *AFLATOXINS , *FEED contamination , *MACROPHAGES , *ELECTROPHORESIS , *HEALTH - Abstract
Abstract: The objective of this study was to evaluate the ability of NovaSil (NS) clay to sorb and mitigate the toxic effects of aflatoxin B1 (AFB1) in Nile tilapia (Oreochromis niloticus). Growth performance, targeted innate immunological function, intestinal microbial community and histology were evaluated after feeding tilapia diets with or without AFB1 and/or NS for 10weeks. Aflatoxin B1 at concentrations of 1.5 and 3.0ppm significantly (P <0.05) decreased weight gain, feed efficiency, hepatosomatic index and macrophage extracellular superoxide anion production in tilapia, regardless of NS addition to the diet. The overall results regarding the efficacy of NS were mixed; however, there was a trend (P =0.157) towards AFB1-toxicity prevention in regards to macrophage extracellular superoxide anion production. Additionally, when 0.5 and 1% NS was included in diets containing 1.5ppm AFB1, total histopathological score was lowered; however, this protective effect was not evident when fish were exposed to 3.0ppm AFB1. Denaturing gradient gel electrophoresis was performed to assess the effects of both AFB1 and NS on gut microbiota, but no significant differences were found among treatment groups. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
36. A novel approach for assessing protein synthesis in channel catfish, Ictalurus punctatus
- Author
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Gasier, Heath G., Previs, Stephen F., Pohlenz, Camilo, Fluckey, James D., Gatlin, Delbert M., and Buentello, J. Alejandro
- Subjects
- *
PROTEIN synthesis , *CHANNEL catfish , *FISH growth , *WATER in the body , *ICTALURUS , *NONINVASIVE diagnostic tests , *DEUTERIUM oxide , *AQUARIUM water - Abstract
Abstract: A comprehensive understanding of animal growth requires adequate knowledge of protein synthesis (PS), which in fish, has traditionally been determined by the flooding dose method. However, this procedure is limited to short-term assessments and may not accurately describe fish growth over extended periods of time. Since deuterium oxide (2H2O) has been used to non-invasively quantify PS in mammals over short- and long-term periods, we aimed at determining if 2H2O could also be used to measure PS in channel catfish. Fish were stocked in a 40-L aquarium with ~4% 2H2O and sampled at 4, 8 and 24h (n =6 at each time period) to determine 2H-labeling of body water (plasma), as well as protein-free and protein-bound 2H-labeled alanine. The labeling of body water reflected that of aquarium water and the labeling of protein-free alanine remained constant over 24h and was ~3.8 times greater than that of body water. By measuring 2H-labeled alanine incorporation after 24h of 2H2O exposure we were able to calculate a rate of PS: 0.04±0.01% h−1. These results demonstrate that PS in fish can be effectively measured using 2H2O and, because this method yields integrative measures of PS, is relatively inexpensive and accounts for perturbations such as feeding, it is a novel and practical assessment option. [Copyright &y& Elsevier]
- Published
- 2009
- Full Text
- View/download PDF
37. Tiny technologies for the global good
- Author
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Court, Erin B., Daar, Abdallah S., Persad, Deepa L., Salamanca-Buentello, Fabio, and Singer, Peter A.
- Published
- 2005
- Full Text
- View/download PDF
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