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Allicin has been reported to play a biological role in human pathophysiological processes via interaction with numerous signaling pathways and gene expression alteration. The purpose of the present study was to evaluate the protective effects of allicin against renal ischemia/reperfusion injury (RIRI) in rats. In the present study, the RIRI model with 45-min ischemia and 22-h reperfusion in rats was generated and allicin was used as the intervention. Changes in renal tissue pathomorphology, renal function, oxidative stress, inflammatory response and apoptosis were evaluated in the RIRI model in rats. Compared with those in the RIRI group, renal function, renal pathological injury, and anti-inflammatory and antioxidant properties were markedly improved in the RIRI+allicin group. Thus, our research suggested that allicin exerted its protective effect against ischemia/reperfusion-induced renal injury by regulating apoptosis, oxidative stress and inflammatory response in rats. [Display omitted] • Pretreatment with allicin could regulate renal function impairment induced by ischemia reperfusion. • Preconditioning with allicin participated in oxidative stress of RIRI. • Allicin played a vital role in RIRI by exerting anti-apoptotic and anti-inflammatory activities. [ABSTRACT FROM AUTHOR]

The main cause of chronic renal allograft dysfunction (CRAD) still remains unclear. Insulin resistance (IR) may be a potential inducement, but there is insufficient evidence about this association. We aimed to establish a rat model of CRAD complicated with IR and to explore the function and pathologic changes of the renal allograft induced by IR. F344-to-Lewis rats of CRAD were fed a high-fat diet to induce IR. They were divided into 3 groups: IR (CRAD+IR), CRAD, and control (CTL). Serum levels of blood urea nitrogen (BUN) and serum creatinine (Scr) were measured to evaluate the renal function. The Homeostasis Model Assessment (HOMA)-IR index was detected by comparing the values of fasting serum insulin levels (FINS) with fasting blood glucose levels (FBG). The pathologic analysis was conducted by the degree of renal lesions including glomerular lesions, renal tubular lesions, hemorrhage, inflammatory cell infiltration, fibrillation, and hyperplasia of the renal interstitium. In the second, third, and fourth month after surgery, serum levels of Scr and BUN in the IR group were reduced more than those in the CRAD group, while they were both higher compared to the CTL group, suggesting that renal function in the CRAD group was declined. The HOMA-IR in the IR group was greater than that in the CRAD and CTL groups, showing that simple high-fat diet feeding significantly and steadily increased FINS and FBG in CRAD complicated with IR rats. Pathologic changes indicated that the CRAD rat model was successfully constructed and was still in the early-middle stages of renal lesions 4 months after surgery, yet IR presented a significant effect on CRAD. These results indicate that the stable CRAD complicated with IR rat model can be established through a high-fat diet in CRAD rats in 4 months, and IR could be an influencing factor. • Stable CRAD complicated with IR rat models can be established via a high-fat diet, in which IR could be an influencing factor. • Simple high-fat diet feeding steadily increased FINS and FBG in rats with CRAD complicated with IR. • HOMA-IR in the IR group was greater than that in the CRAD and CTL groups. [ABSTRACT FROM AUTHOR]

[Display omitted] • Cd inhibited the viability of MDA-MB-231 cells in a time- and dose-dependent manner. • Caspase-inhibitor of z-VAD reduced Cd-induced LDH release and pyroptosis. • NAC attenuated Cd-activated ROS, NLRP3 and inflammation-related genes. • MKK3/6/p38 MAPK/NF-κB pathway was activated. • Cd induced GSDME-dependent pyroptosis by activation of bax/caspase-3/GSDME pathway. Cadmium (Cd) exposure can exert an impact on carcinogenicity of breast cancer, however, the mechanism is not fully understood in triple-negative breast cancer (TNBC). We performed a TNBC MDA-MB-231 cell model and assessed the toxic effect of Cd exposure (0, 10, 20, 50, 60, 80 μM). Cd reduced cell viability in a time- and dose-dependent manner, followed by cell cycle arrest in S phase with alterations of cyclin 1A1, cyclin 1D1 and CDK2. Lactate dehydrogenase (LDH) release, apoptosis and pyroptosis were increased, which were relieved by z-VAD. Elevated ROS and NLRP3, caspase-1, IL-1β and IL-18 were detected, which was attenuated by N-acetylcysteine. Increased bax and decreased caspase-8, caspase-9 and caspase-3 were found. gasdermin E (GSDME) was activated with cleavage of GSDME-NT, which was retarded by z-VAD. Additionally, p38 MAPK signaling pathway was activated. Our data demonstrate GSDME-activated pyroptosis in Cd toxicity, implying a potential impact on TNBC. [ABSTRACT FROM AUTHOR]

Abstract: We have studied the chromosomes of lymphocytes of the residents in a high background radiation area (HBRA) and a control area (CA) in the southern China. The level of natural radiation in HBRA is three to five times higher than that in control area. Unstable type aberrations (dicentrics and rings) were analyzed in 22 individuals in HBRA and 17 individuals in CA. Stable type aberrations (translocations) were examined in 28 elderly individuals and 6 children in HBRA and 24 elderly individuals and 8 children in CA. There was statistically significant difference in the increasing rates between those two areas in adult. In case of stable aberrations, the frequencies were much higher than those of the unstable aberrations both in HBRA and in control. Statistically, there was no difference in the frequencies between HBRA and CA, but the frequencies in children were lower than those in elderly individuals in both groups. Individual variation was small in children while that in adults was large. The amount of chromosome aberrations induced by radiation is not significant when it is compared with that induced by other mutagenic factors such as smoking in the Southern China. [Copyright &y& Elsevier]

The Soil Moisture Experiment in the Luan River (SMELR) was conducted from 2017 to 2018 in the semi-arid Luan River watershed located in the North of China. One of the objectives of SMELR is to serve as an assessment tool and demonstration for a new Terrestrial Water Resources Satellite (TWRS) concept with one-dimensional synthetic aperture microwave techniques, for which soil moisture retrieval under variable satellite observing configurations (mainly in terms of incidence angels) is the greatest challenge. This proposed mission is targeted to provide continuity for the current satellite L-band microwave observations, and further improve the accuracy and spatial resolution of soil moisture mapping through the synergistic use of active, passive and optical remote sensing data. Multi-resolution, multi-angle and multi-spectral airborne data were obtained four times over a 70 km by 12 km area in the Shandian River basin, and one time over a 165 km by 5 km area that includes the Xiaoluan River basin. The near surface soil moisture (0 cm–5 cm) was measured extensively on the ground in fifty 1 km by 2 km quadrats (targeted to compare with the airborne radiometer), and two hundred and fifty 200 m by 200 m quadrats corresponding to radar observations. Two networks were established for continuous measurement of the soil moisture and temperature profile (3 cm, 5 cm, 10 cm, 20 cm, 50 cm) and precipitation in the Shandian and Xiaoluan River basin, respectively. Supporting ground measurements also included ground temperature, vegetation water content, surface roughness, continuous measurement of microwave emission and backscatter at a pasture site, reflectance of various land cover types, evapotranspiration and aerosol observations. Preliminary results within the experimental area indicate that (1) the near surface soil moisture spatial variability at a 200 m scale was up to ~0.1 cm3/cm3 at an intermediate value of ~0.35 cm3/cm3. (2) The difference of soil and vegetation temperature in grass and croplands reach its maximum of 11 K around solar noon time, and the soil temperature gradient is largest at around 15 P.M. (3) Both the airborne and ground measurements cover a wide range of conditions. The L-band active and passive observations exhibit a large variation of ~30 dB and ~80 K, respectively, corresponding to soil moisture range from 0.1 cm3/cm3 to 0.5 cm3/cm3. The sensitivity of both active and passive data to soil moisture is compared at corresponding spatial resolutions and show high information complementarity for better accuracy and resolution soil moisture retrieval. • First airborne optical and active-passive microwave experiment conducted in China • Demonstration of the active-passive microwave synergy at varying incidence angles • Addressing prelaunch science questions of the terrestrial water resources mission [ABSTRACT FROM AUTHOR]