6 results on '"Hasegawa, Daigaku"'
Search Results
2. Development of a novel direct dental pulp-capping material using 4-META/MMA-TBB resin with nano hydroxyapatite
- Author
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Yoshida, Shinichiro, Sugii, Hideki, Itoyama, Tomohiro, Kadowaki, Masataka, Hasegawa, Daigaku, Tomokiyo, Atsushi, Hamano, Sayuri, Ipposhi, Keita, Yamashita, Kozue, and Maeda, Hidefumi
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- 2021
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- View/download PDF
3. Inhibition of c-Jun N-terminal kinase signaling promotes osteoblastic differentiation of periodontal ligament stem cells and induces regeneration of periodontal tissues.
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Kaneko, Hiroshi, Hasegawa, Daigaku, Itoyama, Tomohiro, Yoshida, Shinichiro, Tomokiyo, Atsushi, Hamano, Sayuri, Sugii, Hideki, and Maeda, Hidefumi
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PERIODONTAL ligament , *STEM cells , *REGENERATION (Biology) , *ALVEOLAR process , *BONE regeneration - Abstract
Few clinical treatments to regenerate periodontal tissue lost due to severe endodontic and periodontal disease have yet been developed. Therefore, the development of new treatment methods for the regeneration of periodontal tissue is expected. The purpose of this study was to investigate the effects of a c-Jun N-terminal kinase (JNK) inhibitor, SP600125, on the osteoblastic differentiation of periodontal ligament stem cells (PDLSCs) in vitro, and the function of SP600125 on the regeneration of alveolar bone in vivo. Alizarin red S staining, quantitative RT-PCR, and western blotting analysis was performed to determine whether SP600125 affects osteoblastic differentiation of human PDLSCs (HPDLSCs) and bone-related intracellular signaling. The effect of SP600125 on the regeneration of alveolar bone was assessed by using a rat periodontal defect model. The healing of periodontal defects was evaluated using micro-CT scans and histological analysis. SP600125 promoted the osteoblastic differentiation such as Alizarin red S-positive mineralized nodule formation and the expression of osteoblast-related genes in HPDLSCs under osteogenic conditions. In addition, this inhibitor upregulated the BMP2 expression and the phosphorylation of Smad1/5/8 in HPDLSCs under the same conditions. The inhibition of Smad1/5/8 signaling by LDN193189 suppressed the SP600125-induced osteoblastic differentiation of HPDLSCs. Furthermore, the application of SP600125 promoted the regeneration of not only alveolar bone but also PDL tissue in periodontal defects. This study suggested that inhibition of JNK signaling promotes the osteoblastic differentiation of HPDLSCs through BMP2-Smad1/5/8 signaling, leading to the regeneration of periodontal tissues such as alveolar bone and PDL tissue. • A JNK inhibitor, SP600125, promotes osteoblastic differentiation of PDL stem cells. • Smad1/5/8 mediates SP600125-induced osteoblast differentiation of PDL stem cells. • SP600125 promotes periodontal tissue regeneration at periodontal defects. [ABSTRACT FROM AUTHOR]
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- 2022
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4. Transforming growth factor-β-induced gene product-h3 inhibits odontoblastic differentiation of dental pulp cells.
- Author
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Serita, Suguru, Tomokiyo, Atsushi, Hasegawa, Daigaku, Hamano, Sayuri, Sugii, Hideki, Yoshida, Shinichiro, Mizumachi, Hiroyuki, Mitarai, Hiromi, Monnouchi, Satoshi, Wada, Naohisa, and Maeda, Hidefumi
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ODONTOBLASTS , *DENTAL pulp , *TRANSFORMING growth factors , *CELL differentiation , *SILICATE cements (Dentistry) , *DENTAL extraction - Abstract
Objective The aim of this study was to investigate transforming growth factor-β-induced gene product-h3 (βig-h3) expression in dental pulp tissue and its effects on odontoblastic differentiation of dental pulp cells (DPCs). Design A rat direct pulp capping model was prepared using perforated rat upper first molars capped with mineral trioxide aggregate cement. Human DPCs (HDPCs) were isolated from extracted teeth. βig-h3 expression in rat dental pulp tissue and HDPCs was assessed by immunostaining. Mineralization of HDPCs was assessed by Alizarin red-S staining. Odontoblast-related gene expression in HDPCs was analyzed by quantitative RT-PCR. Results Expression of βig-h3 was detected in rat dental pulp tissue, and attenuated by direct pulp capping, while expression of interleukin-1β and tumor necrosis factor-α was increased in exposed pulp tissue. βig-h3 expression was also detected in HDPCs, with reduced expression during odontoblastic differentiation. The above cytokines reduced βig-h3 expression in HDPCs, and promoted their mineralization. Recombinant βig-h3 inhibited the expression of odontoblast-related genes and mineralization of HDPCs, while knockdown of βig-h3 gene expression promoted the expression of odontoblast-related genes in HDPCs. Conclusions The present findings suggest that βig-h3 in DPCs may be involved in reparative dentin formation and that its expression is likely to negatively regulate this process. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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5. Calcium-sensing receptor-ERK signaling promotes odontoblastic differentiation of human dental pulp cells.
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Mizumachi, Hiroyuki, Yoshida, Shinichiro, Tomokiyo, Atsushi, Hasegawa, Daigaku, Hamano, Sayuri, Yuda, Asuka, Sugii, Hideki, Serita, Suguru, Mitarai, Hiromi, Koori, Katsuaki, Wada, Naohisa, and Maeda, Hidefumi
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G proteins , *BONE morphogenetic proteins , *STRONTIUM , *CALCIUM-sensing receptors , *ODONTOBLASTS - Abstract
Activation of the G protein-coupled calcium-sensing receptor (CaSR) has crucial roles in skeletal development and bone turnover. Our recent study has identified a role for activated CaSR in the osteogenic differentiation of human periodontal ligament stem cells. Furthermore, odontoblasts residing inside the tooth pulp chamber play a central role in dentin formation. However, it remains unclear how CaSR activation affects the odontoblastic differentiation of human dental pulp cells (HDPCs). We have investigated the odontoblastic differentiation of HDPCs exposed to elevated levels of extracellular calcium (Ca) and strontium (Sr), and the contribution of CaSR and the L-type voltage-dependent calcium channel (L-VDCC) to this process. Immunochemical staining of rat dental pulp tissue demonstrated that CaSR was expressed at high levels in the odontoblastic layer, moderate levels in the sublayer, and low levels in the central pulp tissue. Although normal HDPCs expressed low levels of CaSR, stimulation with Ca or Sr promoted both CaSR expression and odontoblastic differentiation of HDPCs along with increased expression of odontoblastic makers. These effects were inhibited by treatment with a CaSR antagonist, whereas treatment with an L-VDCC inhibitor had no effect. Additionally, knockdown of CaSR with siRNA suppressed odontoblastic differentiation of Ca- and Sr-treated HDPCs. ERK1/2 phosphorylation was observed in Ca- and Sr-treated HDPCs, whereas CaSR antagonist treatment or CaSR knockdown blocked ERK1/2 phosphorylation. Furthermore, inhibition of ERK1/2 suppressed mineralization of Ca- and Sr-treated HDPCs. These results suggest that elevated concentrations of extracellular Ca and Sr induce odontoblastic differentiation of HDPCs through CaSR activation and the ERK1/2 phosphorylation. [ABSTRACT FROM AUTHOR]
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- 2017
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6. Effects of Activin A on the phenotypic properties of human periodontal ligament cells.
- Author
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Sugii, Hideki, Maeda, Hidefumi, Tomokiyo, Atsushi, Yamamoto, Naohide, Wada, Naohisa, Koori, Katsuaki, Hasegawa, Daigaku, Hamano, Sayuri, Yuda, Asuka, Monnouchi, Satoshi, and Akamine, Akifumi
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ACTIVIN , *LIGAMENTS , *PERIODONTAL ligament , *BONE physiology , *TOOTH roots - Abstract
Periodontal ligament (PDL) tissue plays an important role in tooth preservation by structurally maintaining the connection between the tooth root and the bone. The mechanisms involved in the healing and regeneration of damaged PDL tissue, caused by bacterial infection, caries and trauma, have been explored. Accumulating evidence suggests that Activin A, a member of the transforming growth factor-β (TGF-β) superfamily and a dimer of inhibinβa, contributes to tissue healing through cell proliferation, migration, and differentiation of various target cells. In bone, Activin A has been shown to exert an inhibitory effect on osteoblast maturation and mineralization. However, there have been no reports examining the expression and function of Activin A in human PDL cells (HPDLCs). Thus, we aimed to investigate the biological effects of Activin A on HPDLCs. Activin A was observed to be localized in HPDLCs and rat PDL tissue. When PDL tissue was surgically damaged, Activin A and IL-1β expression increased and the two proteins were shown to be co-localized around the lesion. HPDLCs treated with IL-1β or TNF-α also up-regulated the expression of the gene encoding inhibinβa. Activin A promoted chemotaxis, migration and proliferation of HPDLCs, and caused an increase in fibroblastic differentiation of these cells while down-regulating their osteoblastic differentiation. These osteoblastic inhibitory effects of Activin A, however, were only noted during the early phase of HPDLC osteoblastic differentiation, with later exposures having no effect on differentiation. Collectively, our results suggest that Activin A could be used as a therapeutic agent for healing and regenerating PDL tissue in response to disease, trauma or surgical reconstruction. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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