1. Evaluation of a new extraction protocol for yeast identification by mass spectrometry.
- Author
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Gouriet, Frédérique, Ghiab, Farida, Couderc, Carine, Bittar, Fadi, Tissot Dupont, Hervé, Flaudrops, Christophe, Casalta, Jean-Paul, Sambe-Ba, Bissoume, Fall, Bécaye, Raoult, Didier, and Fenollar, Florence
- Subjects
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CANDIDA albicans , *PATHOGENIC fungi , *YEAST extract , *FORMIC acid , *MATRIX-assisted laser desorption-ionization , *BIOLOGICAL libraries - Abstract
In this paper, we evaluate a rapid and safe pretreatment procedure using glass beads for MALDI-TOF yeast identification in a routine clinical laboratory avoiding the use of formic acid. We created a new yeast database library using 1186 yeasts, including 11 references strains. The database was tested using 2131 clinical isolates allowing accurate species-level identification in 98.9% (2107/2131) of cases with a score over 1.9 and in 99% (2123/2131) of the strains at the genus level. The new protocol is a rapid, reliable and safe procedure for the accurate identification of pathogenic Candida strains and requires minimal handling. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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