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Due to the features of high porosity, high water content, and tunable viscoelasticity, hydrogels have attracted numerous attentions in the promotion of wound closure. However, the lack of abilities to adapt the wounds of complex shapes and prevent postoperative adhesion limits their therapeutic outcomes in wound healing. To address the above challenges, the supramolecular chitin-based (SMCT) hydrogels are created via the host–guest pre-assembly strategy of β-cyclodextrin (βCD) and adamantane (Ad). The reversible host–guest crosslinks endow the SMCT hydrogels with highly dynamic networks, which can better accommodate irregularly shaped wounds compared with the covalent chitin-based hydrogels with similar mechanical properties. In addition, the SMCT hydrogels show rapid in vivo degradability (degradation time ≈ 2 days) due to the enzyme-triggered degradability of chitin, which do not need to be removed from the wounds after service and thus avoid the secondary damage to skin during dressing change. Owing to the hydrophobic cavity of βCD, the SMCT hydrogels can facilitate the load and release of curcumin with anti-inflammatory, antibacterial, and antioxidative activities, thereby significantly improving the wound healing efficiency. This work provides valuable guidance to the design of self-adaptive and fast-degradable hydrogels that hold great potential for enhancing the wound healing in skin and other tissues. [Display omitted] [ABSTRACT FROM AUTHOR]

Highlights • The probe exhibited good selectivity towards hydrogen polysulfide among other biological species. • The probe can detect hydrogen polysulfide within 10 min. • HQO-PSP has the ability of mitochondrial-targeting. • HQO-PSP can be applied not only at the level of living cells but also to mouse tissues. Abstract Hydrogen polysulfide (H 2 S n) has attracted increasing attention since H 2 S n instead of H 2 S has been shown to be the actual signaling molecule in physiological and pathological processes. Thus, developing accurate and sensitive assays for H 2 S n detection is urgently needed in biological science. In this work, we developed a red emitting mitochondria-targeting fluorescent probe, HQO-PSP, that is capable of tracking H 2 S n. With low cytotoxicity, good selectively and high sensitively, HQO-PSP was able to detect H 2 S n in living cells. Furthermore, we also demonstrated that HQO-PSP could also be successfully applied to recognize H 2 S n in living tissues. [ABSTRACT FROM AUTHOR]

Abstract: The reasonable subject setting of safety engineering has an effect on the culturing of safety talents which is related to the safe operation of our enterprises. Through the use of fuzzy comprehensive evaluation method the mathematical model of the rationality evaluation of talent training mode is established. Taking Kunming University of Science and Technology for example, evaluation indexes of rationality of talent training mode are obtained through the use of fuzzy comprehensive evaluation. It is showed that the highly ambiguous talent training mode can be quantified in accordance with fuzzy comprehensive evaluation model. It provides us with foundation of scheme design of talent training mode. [Copyright &y& Elsevier]

Nanoparticles based on chitosan hydrochloride (CSH)-hyaluronic acid (HA)-PEG were prepared for delivering curcumin (CUR) (CUR-PNPs) to brain tumor. CUR-PNPs of 245.9 nm and spherical morphology were obtained at optimized CSH/HA/PEG20000/CUR ratios with negative charge of about −27.2 mV and EE of approximately 93.3%. Cytotoxicity studies showed that CUR-PNPs improved drug’s anticancer activity in rat glioma cells (C6). The cellular uptake mechanism study showed active targeting of CUR-PNPs into C6 cells by HA mediated endocytosis. Clathrin-coated pit mediated endocytosis, clathrin-mediated endocytosis and macropincytosis were also identified as the entry pathways of PNPs into C6 cells. Pharmacokinetics of preparations in rats after i.v. administration further proved the superiority of CUR-PNPs (3.98 times greater than the area under the curve of CUR solution). In conclusion, CUR-PNPs might be a promising carrier for the therapy of brain tumors. [ABSTRACT FROM AUTHOR]

• The root transcriptomic changes of Shanghaijimaocai (tolerant to salinity) and Te'aiqing (sensitive to salinity) were first investigated. • Osmotic stress-related genes might contribute to the difference in salinity tolerance between Shanghaijimaocai and Te'aiqing. • Ionic -related genes, NHX7 , SLAH1 and ALMT13 might contribute to the difference in salinity tolerance between Shanghaijimaocai and Te'aiqing. Pak choi is an important leafy vegetable crop. Salinity is among the most harmful agents that negatively influence the pak choi yield. However, the mechanism of salinity tolerance in pak choi has not been well understood. In this study, the root transcriptomics of two cultivars differing in salinity tolerance, i.e., Shanghaijimaocai (S, tolerant to salinity) and Te'aiqing (T, sensitive to salinity), were investigated under 0 and 100 mM NaCl treatments. A total of 6765, 2454, 2451 and 5798 differentially expressed unigenes (DEUs) were identified in comparison of S 100 /S 0 , T 100 /T 0 , S 0 /T 0 and S 100 /T 100 , respectively. In Shanghaijimaocai is more sensitive to NaCl stress than Te'aiqing in terms of root transcriptomics. Based on GO and KEGG pathway analyses, the expression levels of several osmotic and ionic stress-related genes, including MP3K18 , PYL8 , PP2C15 / 16 / 49 , ARF2 , bHLH112 , bZIP43 , COL5 , CDF1/3 , ERF25/60 , HSFA6 , MYBS3 / 59 / 92 / CCA1 / PHL5 , POD21 , GOLS7 , CIPK4 / 7 / 12 , NHX7 , SLAH1 and ALMT13 , in Shanghaijimaocai were higher than those in Te'aiqing. Therefore, these genes might contribute to the difference in salinity tolerance. Moreover, the physiological shift of peroxidase activity was in accordance with the dynamic transcript profile of relevant unigenes. These findings would be useful for further functional analysis as potential targets to improve resistance to salinity stress via genetic engineering. [ABSTRACT FROM AUTHOR]