Search

Abstract: Immunophenotyping of lymphoproliferative disorders depends on the effective measurement of cell surface markers. The inherent light-scattering properties of plasmonic nanoparticles (NPs) combined with recent developments in NP design may confer significant advantages over traditional fluorescence probes. We report and evaluate the use of surface-enhanced Raman scattering (SERS) gold NPs (AuNPs) conjugated to therapeutic rituximab antibodies for selective targeting of CD20 molecules. SERS AuNPs were prepared by adsorbing a Raman-active dye onto the surface of 60 nm spherical AuNPs, coating the particles with 5 kDa polyethylene glycol, and conjugating rituximab to functional groups on polyethylene glycol. The effective targeting of CD20 on chronic lymphocytic leukemia cells by rituximab-conjugated SERS AuNPs was evaluated by dark-field imaging, Raman spectroscopy, and flow cytometry with both competitive binding and fluorescence detection procedures. Evidence of CD20 clustering within approximately 100 nm was observed. From the Clinical Editor: This study discusses the use of surface enhancement Raman scattering (SERS)-based plasmonic gold nanoparticles, which can be used for cell specific labeling. In this example rituximab, a commercially available CD20 humanized monoclonal antibody is used. Dark field imaging, Raman spectroscopy and flow cytometry was utilized to demonstrate the sensitive labeling capability of these gold nanoparticle based hybrid nanodevices. [Copyright &y& Elsevier]

Abstract: The present work reports the synthesis and hydrogen storage properties of different types of boron nitride (BN) nanostructures prepared by an in situ silica-assisted catalytic chemical vapor deposition technique. The BN nanostructures have been characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), Brunauer, Emmett, and Teller (BET), Raman spectroscopy and Fourier transform infrared (FTIR) spectroscopy studies. The hydrogen storage properties of BN nanostructures have been investigated using a high-pressure Seiverts'' apparatus in the pressure range of 1–100bar and at 298K. The dependence of hydrogen storage capacity on the morphology of BN nanostructures is discussed in detail. [Copyright &y& Elsevier]

Abstract: Selective targeting and detection of a hematologic malignancy, chronic lymphocytic leukemia, using surface enhanced Raman scattering (SERS) gold nanoparticles is reported. The functional nanoparticles were composed of a gold core onto which an optical reporter dye was adsorbed, protected from aggregation by grafted polyethyleneglycol, and targeted to CD19 antigen by antibodies. The signals were detected by dark-field microscopy and Raman spectrometry. The observation that the Raman signals are not disrupted by several traditional pathology stains indicates advantages over fluorescence methods. [Copyright &y& Elsevier]

The effect of deposition rate of silver (Ag) cathode on the device performance of organic light-emitting diodes (OLEDs) has been investigated. The leaky current before the turn-on of devices was observed in the samples that have the Ag cathode deposited at relatively low rate (less than 0.5 Å/s), and their emission efficiencies were found to be undesirably inconsistent. These issues were not observed in the samples that have Ag cathode deposited at higher rate (>1.0 Å/s). Here we show that the pixel-to-pixel uniformity of OLEDs employing Ag cathode can be simply improved by depositing the cathode at high rate, since the rapid deposition suppresses the Ag diffusion into underlying organic layer. • High thermal deposition rate of Ag cathode could effectively reduce current leakage and improve the emission uniformity for large-area OLEDs. • The low-rate processed Ag cathode could blur the cathode/organic interface and the interfacial metal diffusion was problem trigger. • High-rate processing was more capable to immobilize Ag clusters, and thus refining cathode morphology and structure. [ABSTRACT FROM AUTHOR]