1. Multiple emulsions as effective platforms for controlled anti-cancer drug delivery.
- Author
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Dluska E, Markowska-Radomska A, Metera A, Tudek B, and Kosicki K
- Subjects
- Brain Neoplasms drug therapy, Cell Line, Tumor, Cell Survival, Computer Simulation, Drug Delivery Systems, Drug Liberation, Glioblastoma drug therapy, Humans, Hydrogen-Ion Concentration, Particle Size, Surface Properties, Antineoplastic Agents administration & dosage, Antineoplastic Agents chemistry, Doxorubicin administration & dosage, Doxorubicin chemistry, Emulsions chemistry
- Abstract
Aim: Developing pH-responsive multiple emulsion platforms for effective glioblastoma multiforme therapy with reduced toxicity, a drug release study and modeling., Materials & Methods: Cancer cell line: U87 MG, multiple emulsions with pH-responsive biopolymer and encapsulated doxorubicin (DOX); preparation of multiple emulsions in a Couette-Taylor flow biocontactor, in vitro release study of DOX (fluorescence intensity analysis), in vitro cytotoxicity study (alamarBlue cell viability assay) and numerical simulation of DOX release rates., Results: The multiple emulsions offered a high DOX encapsulation efficiency (97.4 ± 1%) and pH modulated release rates of a drug. Multiple emulsions with a low concentration of DOX (0.02 μM) exhibited broadly advanced cell (U87 MG) cytotoxicity than free DOX solution used at the same concentration., Conclusion: Emulsion platforms could be explored for potential delivery of chemotherapeutics in glioblastoma multiforme therapy.
- Published
- 2017
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