1. Analysis of neutral lipids from microalgae by HPLC-ELSD and APCI-MS/MS
- Author
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Caroline Strub, Angélique Fontana, F. Donot, David Egron, Ziya Günata, J. Malinge, Guillaume Cazals, Sabine Schorr-Galindo, Total SA Pôle d'études et de recherche de Lacq (PERL), TOTAL FINA ELF, Démarche intégrée pour l'obtention d'aliments de qualité (UMR Qualisud), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM), Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM), Total New Energy, Senior Scientific Adviser Chemistry, and Aquatic Phototrophs Coordinator
- Subjects
Clinical Biochemistry ,Analytical chemistry ,APCI-MS/MS ,Atmospheric-pressure chemical ionization ,Fraction (chemistry) ,Mass spectrometry ,01 natural sciences ,Biochemistry ,High-performance liquid chromatography ,Analytical Chemistry ,03 medical and health sciences ,Tandem Mass Spectrometry ,Chromatography detector ,Botryococcus braunii ,Microalgae ,Chromatography, High Pressure Liquid ,030304 developmental biology ,0303 health sciences ,Chromatography ,biology ,Chemistry ,[CHIM.ORGA]Chemical Sciences/Organic chemistry ,Normal phase ,Solid Phase Extraction ,010401 analytical chemistry ,Cell Biology ,General Medicine ,biology.organism_classification ,Lipids ,0104 chemical sciences ,Neutral lipid ,ELSD ,HPLC ,SPE ,Neutral lipids - Abstract
International audience; A method was developed to analyze neutral lipids through the use of three triglycerides, four free fatty acids, six di- and four mono-glycerides standards by high performance liquid chromatography (HPLC) normal phase coupled with either with evaporative light scattering detector (ELSD) or with mass spectrometry (MS) operating in atmospheric pressure chemical ionization (APCI) mode. The method was applied to the determination of the neutral lipid fraction from a Botryococcus braunii race A (B. braunii) culture. This method led us to identify neutral lipids synthesized by B. braunii in a single analysis within 45min through HPLC-APCI-MS/MS technique.
- Published
- 2013
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