Author: "Fabienne Laugerette" / Publisher: hal ccsd - Searchworks@Jio Institute Digital Library Search Results

Your search keyword '"Fabienne Laugerette"' showing total 22 results

Start Over Author "Fabienne Laugerette" Publisher hal ccsd

22 results on '"Fabienne Laugerette"'

1. Milk Polar Lipids in a High-Fat Diet Can Prevent Body Weight Gain: Modulated Abundance of Gut Bacteria in Relation with Fecal Loss of Specific Fatty Acids

Author: David Cheillan, Marie-Caroline Michalski, Karène Bertrand, Charline Buisson, Marine Milard, Pascale Plaisancié, Laure B. Bindels, Florent Joffre, Sébastien Viel, Valérie Sauvinet, Nathalie M. Delzenne, Audrey M. Neyrinck, Emmanuelle Loizon, Lydie Humbert, Annie Durand, Corinne Louche-Pelissier, Emmanuelle Meugnier, Dominique Rainteau, Fabienne Laugerette, Lorna Garnier, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL), Center for Computational Imaging and Simulation Technologies in Biomedicine (CISTIB), Universitat Pompeu Fabra [Barcelona] (UPF), Louvain Drug Research Institute, Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RA), Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-CHU Grenoble-Hospices Civils de Lyon (HCL)-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Service d'immunologie [Centre Hospitalier Lyon Sud - HCL], Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Réponse immunitaire innée dans les maladies infectieuses et auto-immunes – Innate immunity in infectious and autoimmune diseases, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut des Corps Gras (ITERG), ITERG, Laboratoire des biomolécules (LBM UMR 7203), Chimie Moléculaire de Paris Centre (FR 2769), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Ecole Superieure de Physique et de Chimie Industrielles de la Ville de Paris (ESPCI Paris), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Institut de Chimie du CNRS (INC)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Département de Chimie - ENS Paris, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Nutrition and Metabolism Research Group (LDRI), Université Catholique de Louvain = Catholic University of Louvain (UCL), French Ministry for Higher Education and Research via EDISS, Universite Claude Bernard Lyon 1, CNIEL (French Dairy Interbranch Organization), Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RH), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-CHU Saint-Etienne-Hospices Civils de Lyon (HCL)-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Centre International de Recherche en Infectiologie - UMR (CIRI), École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École normale supérieure - Paris (ENS Paris)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP)-ESPCI ParisTech-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure - Paris (ENS Paris)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP)-ESPCI ParisTech-Centre National de la Recherche Scientifique (CNRS)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Université Catholique de Louvain (UCL), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-CHU Saint-Etienne-Hospices Civils de Lyon (HCL)-CHU Grenoble, Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Département de Chimie - ENS Paris, École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Chimie Moléculaire de Paris Centre (FR 2769), Institut de Chimie du CNRS (INC)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), and Michalski, Marie-Caroline
Subjects: Male, 0301 basic medicine, obesity, sphingomyeline, [SDV]Life Sciences [q-bio], Ingénierie des aliments, Gut flora, Weight Gain, [SDV.IDA]Life Sciences [q-bio]/Food engineering, adiposity, bile salts, feces, gut barrier, microbiota, milk fat globule membrane, phospholipids, sphingomyelin, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, biology, acide gras, Chemistry, Fatty Acids, sel biliaire, Lipids, Sphingomyelins, Bifidobacterium animalis, obésité, Milk, Liver, medicine.symptom, Sphingomyelin, Biotechnology, phospholipide, medicine.medical_specialty, Crypt, Inflammation, Diet, High-Fat, 03 medical and health sciences, microbiote, lipid, Internal medicine, medicine, Food engineering, Animals, Feces, lipide, 030109 nutrition & dietetics, Macrophages, barrière intestinale, biology.organism_classification, medicine.disease, Obesity, Gastrointestinal Microbiome, Lactobacillus reuteri, Mice, Inbred C57BL, 030104 developmental biology, Endocrinology, Intestinal Absorption, fatty acid, Food Science
Abstract: Scope Enhanced adiposity and metabolic inflammation are major features of obesity associated with altered gut microbiota and intestinal barrier. How these metabolic outcomes can be impacted by milk polar lipids (MPL), naturally containing 25% of sphingomyelin, is investigated in mice fed a mixed high-fat (HF) diet . Methods and results Male C57Bl/6 mice receive a HF-diet devoid of MPL (21% fat, mainly palm oil, in chow), or supplemented with 1.1% or 1.6% of MPL (HF-MPL1; HF-MPL2) via a total-lipid extract from butterserum concentrate for 8 weeks. HF-MPL2 mice gain less weight versus HF (p < 0.01). Diets do not impact plasma markers of inflammation but in the liver, HF-MPL2 tends to decrease hepatic gene expression of macrophage marker F4/80 versus HF-MPL1 (p = 0.06). Colonic crypt depth is the maximum in HF-MPL2 (p < 0.05). In cecal microbiota, HF-MPL1 increases Bifidobacterium animalis versus HF (p < 0.05). HF-MPL2 decreases Lactobacillus reuteri (p < 0.05), which correlates negatively with the fecal loss of milk sphingomyelin-specific fatty acids (p < 0.05). Conclusion In mice fed a mixed HF diet, MPL can limit HF-induced body weight gain and modulate gut physiology and the abundance in microbiota of bacteria of metabolic interest. This supports further exploration of how residual unabsorbed lipids reaching the colon can impact HF-induced metabolic disorders.
Published: 2019

2. Acute effects of milk polar lipids on intestinal tight junction expression: Towards an impact of sphingomyelin through the regulation of IL-8 secretion?

Author: David Cheillan, Karène Bertrand, Emmanuelle Loizon, Lorna Garnier, Fabienne Laugerette, Florent Joffre, Charline Buisson, Marie-Caroline Michalski, Sébastien Viel, Armelle Penhoat, Marine Milard, Annie Durand, Emmanuelle Meugnier, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), ITERG, Service de Biochimie et Biologie Moléculaire Grand Est, Centre de Biologie et de Pathologie Est[Lyon], Hospices Civils de Lyon (HCL), Département d'allergie et d'immunologie clinique [CHU Lyon Sud], Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL)-Hospices Civils de Lyon (HCL), Réponse immunitaire innée dans les maladies infectieuses et auto-immunes – Innate immunity in infectious and autoimmune diseases, Centre International de Recherche en Infectiologie (CIRI), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Service d'immunologie [Centre Hospitalier Lyon Sud - HCL], French Ministry for Higher Education and Research via EDISS, Universite Claude Bernard Lyon 1, CNIEL (French Dairy Interbranch Organization), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Centre International de Recherche en Infectiologie - UMR (CIRI), Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Institut National de la Recherche Agronomique (INRA)
Subjects: Male, 0301 basic medicine, Chemokine CXCL1, Endocrinology, Diabetes and Metabolism, [SDV]Life Sciences [q-bio], Chemokine CXCL2, Clinical Biochemistry, Occludin, Biochemistry, Tight Junctions, Proinflammatory cytokine, 03 medical and health sciences, Gene expression, cytokine, Animals, Humans, Secretion, Molecular Biology, reproductive and urinary physiology, phospholipid, Tight Junction Proteins, 030109 nutrition & dietetics, Nutrition and Dietetics, Dose-Response Relationship, Drug, Tight junction, gut barrier, Chemistry, Interleukin-8, virus diseases, Lipid metabolism, Lipids, Molecular biology, Sphingomyelins, Intestines, Mice, Inbred C57BL, Milk, 030104 developmental biology, Gene Expression Regulation, Caco-2, caco-2, dairy, Caco-2 Cells, Sphingomyelin, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, milk fat globule membrane
Abstract: International audience; Milk polar lipids (MPL) are specifically rich in milk sphingomyelin (MSM) which represents 24% of MPL. Beneficial effects of MPL or MSM have been reported on lipid metabolism, but information on gut physiology is scarce. Here we assessed whether MPL and MSM can impact tight junction expression. Human epithelial intestinal Caco-2/TC7 cells were incubated with mixed lipid micelles devoid of MSM (Control) or with 0.2 or 0.4 mM of MSM via pure MSM or via total MPL. C57Bl/6 mice received 5 or 10 mg of MSM via MSM or via MPL (oral gavage); small intestinal segments were collected after 4 h. Impacts on tight junction and cytokine expressions were assessed by qPCR; IL-8 and IL-8 murine homologs (Cxcl1, Cxcl2) were analyzed. In vitro, MSM increased tight junction expression (Occludin, ZO-1) vs Control, unlike MPL. However, no differences were observed in permeability assays (FITC-dextran, Lucifer yellow). MSM increased the secretion and gene expression of IL-8 but not of other inflammatory cytokines. Moreover, cell incubation with IL-8 induced an overexpression of tight junction proteins. In mice, mRNA level of Cxcl1 and Cxcl2 in the ileum were increased after gavage with MSM vs NaCl but not with MPL. Altogether, these results suggest a specific action of MSM on intestinal tight junction expression, possibly mediated by IL-8. Our study provides clues to shed light on the beneficial effects of MPL on intestinal functions and supports the need for further mechanistic exploration of the direct vs indirect effects of MSM and IL-8 on the gut barrier.
Published: 2019

3. Metabolic endotoxemia: a potential underlying mechanism of the relationship between dietary fat intake and risk for cognitive impairments in humans?

Author: Catherine Féart, Perrine André, Fabienne Laugerette, Astrophysique Interprétation Modélisation (AIM (UMR_7158 / UMR_E_9005 / UM_112)), Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris Diderot - Paris 7 (UPD7), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut de Santé Publique, d'Epidémiologie et de Développement (ISPED), Université Bordeaux Segalen - Bordeaux 2, Astrophysique Interprétation Modélisation (AIM (UMR7158 / UMR_E_9005 / UM_112)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), and Andre, Perrine
Subjects: Male, microbiome, Bioinformatics, Metabolic endotoxemia, Cognition, 0302 clinical medicine, Risk Factors, Medicine, high-fat, 10. No inequality, Cognitive impairment, humans, 2. Zero hunger, matière grasse alimentaire, Nutrition and Dietetics, endotoxemia, Communication, lipopolysaccharide, Age Factors, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, Middle Aged, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, Prognosis, 3. Good health, maladie d'alzheimer, facteur de risque, nutrition, dietary fat, Host-Pathogen Interactions, Alimentation et Nutrition, Female, lcsh:Nutrition. Foods and food supply, Nutritive Value, Alzheimer’s disease, Adult, lcsh:TX341-641, 030209 endocrinology & metabolism, valeur nutritive, Risk Assessment, personne âgée, Young Adult, 03 medical and health sciences, Healthy food, Alzheimer's disease, dementia, Dementia, Food and Nutrition, Cognitive Dysfunction, Aged, Bacteria, démence, business.industry, Mechanism (biology), interaction hôte pathogène, lipopolisaccharide, medicine.disease, Dietary Fats, Gastrointestinal Microbiome, Dietary fat intake, Cognitive Aging, [SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie, Healthy ageing, business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 030217 neurology & neurosurgery, Food Science
Abstract: (1) Background: Nutrition is a major lifestyle factor that can prevent the risk of cognitive impairment and dementia. Diet-induced metabolic endotoxemia has been proposed as a major root cause of inflammation and these pathways emerge as detrimental factors of healthy ageing. The aim of this paper was to update research focusing on the relationship between a fat-rich diet and endotoxemia, and to discuss the potential role of endotoxemia in cognitive performances. (2) Methods: We conducted a non-systematic literature review based on the PubMed database related to fat-rich meals, metabolic endotoxemia and cognitive disorders including dementia in humans. A total of 40 articles out of 942 in the first screening met the inclusion criteria. (3) Results: Evidence suggested that a fat-rich diet, depending on its quality, quantity and concomitant healthy food components, could influence metabolic endotoxemia. Since only heterogeneous cross-sectional studies are available, it remains unclear to what extent endotoxemia could be associated or not with cognitive disorders and dementia. (4) Conclusions: A fat-rich diet has the capability to provide significant increases in circulating endotoxins, which highlights nutritional strategies as a promising area for future research on inflammatory-associated diseases. The role of endotoxemia in cognitive disorders and dementia remains unclear and deserves further investigation.
Published: 2019

4. Metabolic effects in mice of cream formulation: Addition of both thickener and emulsifier does not alter lipid metabolism but modulates mucus cells and intestinal endoplasmic reticulum stress

Author: Charline Buisson, Pascale Plaisancié, S. Bugeat, Marion Létisse, Emmanuelle Loizon, Alain Géloën, S. Serieye, Emmanuelle Meugnier, Marie-Caroline Michalski, Annie Durand, Fabienne Laugerette, Marine Milard, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Laboratoire de Spectrochimie Infrarouge et Raman - UMR 8516 (LASIR), Centre National de la Recherche Scientifique (CNRS)-Université de Lille, Laboratoire d'ingénierie circulation transports (LICIT), Institut National de Recherche sur les Transports et leur Sécurité (INRETS)-École Nationale des Travaux Publics de l'État (ENTPE), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Sodiaal Union, Partenariat industriel : SODIAAL-CANDIA R&D; Sodiaal-Candia RD, Sodiaal-Candia RI, CNIEL (French Dairy Interbranch Organization, Paris, France), Nutricia Research (Utrecht, the Netherlands), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Laboratoire Avancé de Spectroscopie pour les Intéractions la Réactivité et l'Environnement - UMR 8516 (LASIRE), Ecole Nationale Supérieure de Chimie de Lille (ENSCL)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), and ProdInra, Migration
Subjects: Male, 0301 basic medicine, medicine.medical_specialty, Duodenum, [SDV]Life Sciences [q-bio], cow milk, Fatty Acids, Nonesterified, dairy matrix, Mice, 03 medical and health sciences, chemistry.chemical_compound, Internal medicine, Intestine, Small, Genetics, medicine, Animals, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, Intestinal permeability, Chemistry, Endoplasmic reticulum, Intestinal lipid absorption, Lipid metabolism, Endoplasmic Reticulum Stress, Lipid Metabolism, medicine.disease, Mucus, Small intestine, 3. Good health, Carrageenan, Mice, Inbred C57BL, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Milk, nutrition, 030104 developmental biology, medicine.anatomical_structure, Endocrinology, Liver, Emulsifying Agents, carrageenan, Cattle, Food Additives, Animal Science and Zoology, Dairy Products, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract: International audience; Additives stabilize or improve the organoleptic or functional properties (or both) of many dairy products including whipping cream. Their influence on the metabolic effect of dairy cream is scarcely known. We tested the hypothesis that added emulsifier (lactic acid esters of mono- and diglycerides; MAG/DAG), thickener (carrageenan, CGN), or both, could modify the metabolic effect, notably in the intestine and liver. Nine-week-old male C57Bl/6J mice were fed UHT cream (indirect treatment) mixed with nonlipidic powder (final: 13% milkfat) for 1 or 4 wk. We compared creams (1) without additive (Ctl), (2) with thickener (Th), 0.02% of κ-CGN, and (3) with both thickener and emulsifier, 0.1% of MAG/DAG esters (Th/Em). We analyzed plasma parameters, intestine, and liver. Fasting glycemia, insulinemia, triglyceridemia, nonesterified fatty acids, body weight gain, and liver weight did not differ among groups. After 1 wk, Th/Em had higher expression in the duodenum of some of the genes involved in (1) intestinal lipid absorption and (2) tight junction proteins versus Ctl and Th. After 4 wk, mucus cell number in the small intestine was higher in Th/Em versus Ctl and Th. Genes involved in endoplasmic reticulum (ER) stress in the duodenum were more expressed in Th/Em after 1 wk. After 4 wk, in the colon, a higher expression of ER stress genes was observed for Th versus Th/Em and Ctl. Liver damage score was not altered by additives. Adding both CGN (0.02%) and MAG/DAG esters (0.1%) in dairy cream did not result in deleterious outcomes in mice after 4 wk regarding lipid metabolism, intestinal permeability, and liver disorders. The longer term effect of intestinal ER stress modulation deserves further investigation.
Published: 2018

5. Metabolic effects in mice of cream processing: Direct ultra-high-temperature process lowers high-fat-induced adipose tissue inflammation

Author: Marie-Caroline Michalski, Alain Géloën, Emmanuelle Meugnier, Charline Buisson, Pascale Plaisancié, Marion Létisse, Emmanuelle Loizon, S. Serieye, S. Bugeat, Fabienne Laugerette, Annie Durand, Marine Milard, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Sodiaal Union, Partenariat industriel : SODIAAL-CANDIA R&D; Sodiaal-Candia R&I (Research & Innovation Department, Paris, France), Sodiaal-Candia RI, CNIEL (French Dairy Interbranch Organization, Paris, France), and Nutricia Research (Utrecht, the Netherlands)
Subjects: Male, 0301 basic medicine, medicine.medical_specialty, Hot Temperature, cow milk, Adipose tissue, Inflammation, dairy matrix, Fatty acid-binding protein, Fats, Butterfat, Mice, 03 medical and health sciences, chemistry.chemical_compound, Internal medicine, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Gene expression, Genetics, medicine, Animals, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, ComputingMilieux_MISCELLANEOUS, Epididymis, 2. Zero hunger, 030109 nutrition & dietetics, Chemistry, Lipid metabolism, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Carrageenan, Intestines, Mice, Inbred C57BL, Milk, nutrition, Endocrinology, Adipose Tissue, Zonula Occludens-1 Protein, Microsome, Cattle, Animal Science and Zoology, Dairy Products, medicine.symptom, food process, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract: International audience; Although UHT heat treatment is being optimized to improve the stability and functional properties of dairy products, its metabolic effects remain scarcely known. As such, we studied the effect of the type of UHT process on lipid metabolism, intestinal barrier, and inflammation in mice. Nine-week-old male C57Bl/6J mice were fed a diet composed of nonlipidic powder mixed with different UHT dairy creams (final: 13% milkfat) for 1 or 4 wk. All creams contained 0.02% of thickener (carrageenan) and were treated via either (1) classical indirect heating process (Th), (2) indirect process at higher temperature (Th+), or (3) direct process by steam injection (ThD). Plasma, epididymal adipose tissue (EAT), and intestine were analyzed. Multivariate principal component analyses were used to identify differential effects of processes. Th+ differed by a globally higher liver damage score compared with that of the other creams. After 4 wk, the duodenal expression of lipid absorption genes fatty acid binding protein 4 (Fatp4) and microsomal triglycerides transfer protein (Mttp) was lower in the Th+ versus Th group. Expression in the colon of tight junction protein zonula occludens 1 (Zo-1) and of some endoplasmic reticulum stress markers was lower in both Th+ and ThD versus the Th group. In EAT, ThD had lower gene expression of several inflammatory markers after 4 wk. Some differential effects may be related to heat-induced physicochemical changes of creams. The type of cream UHT process differentially affected metabolic parameters in mice after a 4-wk fat-rich diet, partly due to cream structure. Altogether, direct steam injection process induced the lowest early markers of high-fat-induced metabolic inflammation in EAT.
Published: 2018

6. Increased jejunal permeability in human obesity is revealed by a lipid challenge and is linked to inflammation and type 2 diabetes

Author: Laurent, Genser, Doriane, Aguanno, Hédi A, Soula, Liping, Dong, Laurence, Trystram, Karen, Assmann, Joe-Elie, Salem, Jean-Christophe, Vaillant, Jean-Michel, Oppert, Fabienne, Laugerette, Marie-Caroline, Michalski, Philippe, Wind, Monique, Rousset, Edith, Brot-Laroche, Armelle, Leturque, Karine, Clément, Sophie, Thenet, Christine, Poitou, CHU Pitié-Salpêtrière [APHP], Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche des Cordeliers (CRC (UMR_S_1138 / U1138)), École pratique des hautes études (EPHE)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Unité de Recherche en Epidémiologie Nutritionnelle (UREN), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Sorbonne Paris Cité (USPC)-Université Paris 13 (UP13)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Institut National de la Recherche Agronomique (INRA), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Norwegian Meteorological Institute, Institut de Physique du Globe de Paris (IPGP), Institut national des sciences de l'Univers (INSU - CNRS)-IPG PARIS-Université Paris Diderot - Paris 7 (UPD7)-Université de La Réunion (UR)-Centre National de la Recherche Scientifique (CNRS), Université Paris 13 (UP13)-Institut National de la Recherche Agronomique (INRA)-Conservatoire National des Arts et Métiers [CNAM] (CNAM)-Université Sorbonne Paris Cité (USPC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Université de La Réunion (UR)-Université Paris Diderot - Paris 7 (UPD7)-IPG PARIS-Institut national des sciences de l'Univers (INSU - CNRS), CHU Pitié-Salpêtrière [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Norwegian Meteorological Institute [Oslo] (MET), CAN Institute for transversal leaky gut project, Benjamin Delessert Institute, Groupe Lipides et Nutrition (GLN), Appert Institute (UPPIA), Fondation Obelisque, Fondation Nestle, Societe Francaise et Francophone de Chirurgie de l'Obesite et des Maladies Metaboliques (SOFFCO-MM), Societe Francaise de Chirurgie Digestive (SFCD), EU-project Metacardis HEALTH-F4-2012-305312, Institut National de la Sante et de la Recherche Medicale, Universite Pierre et Marie Curie, Ecole Pratique des Hautes Etudes, and CORDDIM Ile de France
Subjects: Adult, Male, Cholera Toxin, obesity, [SDV]Life Sciences [q-bio], adipose-tissue, Permeability, weight-reduction, intestinal barrier function, Tight Junctions, insulin-resistance, lipids, Young Adult, Occludin, Pathology, Humans, Protein Precursors, jejunum, gastric bypass, Micelles, Aged, disease, Membrane Glycoproteins, Haptoglobins, gut microbiota, intestinal permeability, endotoxemia, chamber, Middle Aged, MARVEL Domain Containing 2 Protein, high-fat diet, Diabetes Mellitus, Type 2, Intestinal Absorption, Oncology, inflammation, Case-Control Studies, tight junction proteins, Female, type 2 diabetes, Caco-2 Cells, Ussing, Carrier Proteins, Acute-Phase Proteins, tricellular tight junctions
Abstract: International audience; Obesity and its metabolic complications are characterized by subclinical systemic and tissue inflammation. In rodent models of obesity, inflammation and metabolic impairments are linked with intestinal barrier damage. However, whether intestinal permeability is altered in human obesity remains to be investigated. In a cohort of 122 severely obese and non-obese patients, we analyzed intestinal barrier function combining in vivo and ex vivo investigations. We found tight junction impairments in the jejunal epithelium of obese patients, evidenced by a reduction of occludin and tricellulin. Serum levels of zonulin and LPS binding protein, two markers usually associated with intestinal barrier alterations, were also increased in obese patients. Intestinal permeability per se was assessed in vivo by quantification of urinary lactitol/mannitol (L/M) and measured directly ex vivo on jejunal samples in Ussing chambers. In the fasting condition, L/M ratio and jejunal permeability were not significantly different between obese and non-obese patients, but high jejunal permeability to small molecules (0.4kDa) was associated with systemic inflammation within the obese cohort. Altogether, these results suggest that intestinal barrier function is subtly compromised in obese patients. We thus tested whether this barrier impairment could be exacerbated by dietary lipids. To this end, we challenged jejunal samples with lipid micelles and showed that a single exposure increased permeability to macromolecules (4kDa). Jejunal permeability after the lipid load was two-fold higher in obese patients compared to non-obese controls and correlated with systemic and intestinal inflammation. Moreover, lipid-induced permeability was an explicative variable of type 2 diabetes. In conclusion, intestinal barrier defects are present in human severe obesity and exacerbated by a lipid challenge. This paves the way to the development of novel therapeutic approaches to modulate intestinal barrier function or personalize nutrition therapy to decrease lipid-induced jejunal leakage in metabolic diseases. Copyright (c) 2018 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Published: 2018

7. Emulsifying dietary fat modulates postprandial endotoxemia associated with chylomicronemia in obese men: a pilot randomized crossover study

Author: Martine Laville, Hubert Vidal, Cécile Vors, Jocelyne Drai, Fabienne Laugerette, Gaëlle Pineau, Marie-Caroline Michalski, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA), Centre National Interprofessionnel de l'Economie Laitiere (CNIEL, French Dairy Board), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL)
Subjects: 0301 basic medicine, Male, IMPACT, Endocrinology, Diabetes and Metabolism, [SDV]Life Sciences [q-bio], Clinical Biochemistry, endotoxine, hyperlipemie, 0302 clinical medicine, Endocrinology, Ingestion, lcsh:RC620-627, 2. Zero hunger, Meal, Cross-Over Studies, digestive, oral, and skin physiology, Postprandial Period, homme, 3. Good health, lcsh:Nutritional diseases. Deficiency diseases, obésité, Postprandial, facteur de risque, Alimentation et Nutrition, Hyperlipoproteinemia Type I, TRIAL, MEAL, Lipidology, Adult, medicine.medical_specialty, Short Report, 030209 endocrinology & metabolism, Clinical nutrition, 03 medical and health sciences, Insulin resistance, INFLAMMATION, Internal medicine, medicine, Humans, Food and Nutrition, Obesity, 030109 nutrition & dietetics, business.industry, LIPOPOLYSACCHARIDES, Biochemistry (medical), medicine.disease, Crossover study, Dietary Fats, Endotoxemia, business, graisse alimentaire
Abstract: Postprandial hyperlipemia is recognized as a major cardio-metabolic risk factor, recently linked to the co-absorption of pro-inflammatory lipopolysaccharides with dietary lipids. This causes endotoxemia that is involved in the pathophysiology of obesity and insulin resistance, but to date the impact of food formulation is unknown. We tested a novel concept that endotoxin absorption can be modulated by fat emulsified structure in the meal, and potentially differently in obese vs. lean men. In a randomized controlled crossover study, eight normal-weight and eight obese age-matched healthy men ingested two isocaloric, isolipidic breakfasts of identical composition including 40 g of milk fat that was emulsified or unemulsified. Plasma- and chylomicron-endotoxemia and chylomicron-triglycerides were measured during 8 h after breakfast ingestion. After emulsion consumption, parallel to an enhanced chylomicronemia, obese subjects presented an early and sharp increase in chylomicron-endotoxemia at 60 min (P time = 0.02), which was higher than (i) after spread fat in obese subjects (P
Published: 2017

8. L’hyperphagie transitoire lors de la consommation d’un régime de type occidental est nécessaire au contrôle de l’endotoxémie métabolique à moyen terme chez le rat

Author: Mathilde Guerville, Anaïs Leroy, Annaëlle Sinquin, Fabienne Laugerette, Marie-Caroline Michalski, Gaelle Boudry, Nutrition, Métabolismes et Cancer (NuMeCan), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), ProdInra, Archive Ouverte, and Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract: National audience; L’hyperphagie transitoire lors de la consommation d’un régime de type occidental est nécessaire au contrôle de l’endotoxémie métabolique à moyen terme chez le rat. 34. Congrès du club d'étude des cellules épithéliales digestives (CECED)
Published: 2017

9. Dietary emulsifiers from milk and soybean differently impact adiposity and inflammation in association with modulation of colonic goblet cells in high-fat fed mice

Author: Annie Durand, Carole Vaysse, Marion Létisse, Pascale Plaisancié, Gaëlle Pineau, Armelle Penhoat, Alain Géloën, Marie-Caroline Michalski, Florent Joffre, Manon Lecomte, Fabienne Laugerette, Leslie Couëdelo, Laure Gabert, Emmanuelle Meugnier, Bérengère Benoit, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Region Rhone-Alpes ARC1, Lipides pour l'Industrie et la Sante, Societe Francophone du Diabete, Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Institut des Corps Gras (ITERG), Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RH), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-CHU Saint-Etienne-Hospices Civils de Lyon (HCL)-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université Panthéon-Sorbonne - UFR d'Économie (UP1 UFR02), Université Panthéon-Sorbonne (UP1), ITERG (ITERG), ITERG, Régulations métaboliques, nutrition et diabètes (RMND), Oméga 21, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), and Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Male, Panniculitis, [SDV]Life Sciences [q-bio], Adipose tissue, Metabolic disease, White adipose tissue, chemistry.chemical_compound, Feces, Intestinal mucosa, Gut barrier, Lecithins, Diet, Fat-Restricted, ComputingMilieux_MISCELLANEOUS, Adiposity, 2. Zero hunger, chemistry.chemical_classification, Leptin, Lipids, 3. Good health, Phospholipid, Milk, Liver, Tumor necrosis factor alpha, Goblet Cells, medicine.symptom, Biotechnology, medicine.medical_specialty, Colon, Adipose Tissue, White, Inflammation, Biology, Diet, High-Fat, 03 medical and health sciences, Internal medicine, medicine, Animals, Humans, 030109 nutrition & dietetics, Fatty acid, Caco-2, Mice, Inbred C57BL, Endocrinology, chemistry, Emulsifying Agents, Immunology, Soybeans, Caco-2 Cells, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract: International audience; SCOPE: Enhanced adiposity and metabolic inflammation are major features of obesity that could be impacted by dietary emulsifiers. We investigated in high-fat fed mice the effects of using a new polar lipid (PL) emulsifier from milk (MPL) instead of soybean lecithin (soybean PL [SPL]) on adipose tissue and intestinal mucosa function. METHODS AND RESULTS: Four groups of C57BL6 mice received for 8 wks a low-fat (LF) diet or a high-fat diet devoid of PLs or an high-fat diet including MPL (high-fat-MPL) or SPL (high-fat-SPL). Compared with high-fat diet, high-fat-SPL diet increased white adipose tissue (WAT) mass (p \textless 0.05), with larger adipocytes (p \textless 0.05) and increased expression of tumor necrosis factor alpha, monochemoattractant protein-1, LPS-binding protein, and leptin (p \textless 0.05). This was not observed with high-fat-MPL diet despite similar dietary intakes and increased expression of fatty acid transport protein 4 and microsomal TG transfer protein, involved in lipid absorption, in upper intestine (p \textless 0.05). High-fat-MPL mice had a lower expression in WAT of cluster of differentiation 68, marker of macrophage infiltration, versus high-fat and high-fat-SPL mice (p \textless 0.05), and more goblet cells in the colon (p \textless 0.05). CONCLUSIONS: Unlike SPL, MPL in the high-fat diet did not induce WAT hypertrophy and inflammation but increased colonic goblet cells. This supports further clinical exploration of different sources of dietary emulsifiers in the frame of obesity outbreak.
Published: 2016

10. Dietary lipid emulsions and endotoxemia

Author: Marie-Caroline Michalski, Fabienne Laugerette, Manon Lecomte, Cécile Vors, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Centre Européen pour la Nutrition et la Santé (CENS), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), ProdInra, Archive Ouverte, and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: 0301 basic medicine, medicine.medical_specialty, obesity, [SDV]Life Sciences [q-bio], Dietary lipid, lcsh:TP670-699, 030209 endocrinology & metabolism, Inflammation, Biology, oil, Biochemistry, Intestinal absorption, fat, emulsifier, inflammation, nutrition, Metabolic endotoxemia, 03 medical and health sciences, 0302 clinical medicine, Insulin resistance, Internal medicine, pétrole, medicine, human nutrition, 2. Zero hunger, petroleum, 030109 nutrition & dietetics, Intestinal lipid absorption, émulsifiant, Transporter, medicine.disease, graisse, 3. Good health, [SDV] Life Sciences [q-bio], obésité, Postprandial, Endocrinology, Immunology, lipids (amino acids, peptides, and proteins), lcsh:Oils, fats, and waxes, medicine.symptom, Agronomy and Crop Science, nutrition humaine, Food Science
Abstract: The low-grade inflammation observed in obesity is a risk factor for cardiovascular diseases and insulin resistance. Among factors triggering such inflammation, recent works revealed the role of bacterial lipopolysaccharides (LPS), so-called endotoxins. LPS are naturally present in the gut via the intestinal microbiota. Recent studies show that they can induce in plasma a metabolic endotoxemia after the consumption of unbalanced hyperlipidic meals. This article reviews recent knowledge gained on the role of intestinal lipid absorption and the composition of dietary lipids on: (i) the induction of metabolic endotoxemia, (ii) the types of plasma transporters of LPS and (iii) associated low-grade inflammation. Notably, lipids are present in foods under various physicochemical structures and notably in emulsified form. Our recent works reveal that such structure and the type of emulsifier can modulate postprandial lipemia; recent results on the possible consequences on metabolic endotoxemia will be discussed.
Published: 2016

11. L’hyperphagie transitoire lors de la consommation d’un régime de type occidental est nécessaire aucontrôle de l’endotoxémie métabolique à moyen terme

Author: Fabienne Laugerette, Marie-Caroline Michalski, Mathilde Guerville, Gaëlle Boudry, A. Sinquin, Nutrition, Métabolismes et Cancer (NuMeCan), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Société Française de Nutrition (SFN). FRA., Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), ProdInra, Archive Ouverte, and Université de Rennes (UR)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: [SDV] Life Sciences [q-bio], 0303 health sciences, 03 medical and health sciences, 0302 clinical medicine, Nutrition and Dietetics, 030309 nutrition & dietetics, Endocrinology, Diabetes and Metabolism, [SDV]Life Sciences [q-bio], Internal Medicine, 030209 endocrinology & metabolism
Abstract: Introduction et but de l’etude La presence dans le sang de lipopolysaccharides (LPS) ou endotoxemie metabolique, liee a une alteration de la barriere intestinale, est impliquee dans le developpement de l’inflammation chronique de bas-grade caracteristique de l’obesite. La consommation d’un regime de type occidental riche en lipides et pauvre en fibres induit une augmentation des taux de LPS circulant mais aussi une hyperphagie transitoire. L’objectif de notre etude etait d’evaluer le role de l’hyperphagie liee a la consommation d’un tel regime dans la cinetique d’alteration des mecanismes regulant la translocation caecale et ileale du LPS. Materiel et methodes Vingt-quatre rats mâles Wistar ont ete nourris avec un regime standard (C) ou un regime Western ad libitum (WD al ) riche en lipides (45 % de l’energie) et appauvri en fibre durant 1 ou 6 semaines. Un troisieme groupe a ete nourri avec l’aliment Western mais avec un apport calorique au meme niveau que les animaux temoins ( pair-feeding , WD pf , n = 12). A l’euthanasie, le passage ileal et caecal de LPS a ete mesure en chambres d’Ussing. L’endotoxemie a ete evaluee par dosage LAL, l’activite de la phosphatase alcaline intestinale (IAP) par dosage colorimetrique et l’expression de molecules impliquees dans la signalisation du LPS ( tlr4, md2, myd88 ) par PCR. Resultats et analyse statistique L’adiposite a 6 semaines etait augmentee chez les animaux nourris avec le regime WD, ad libitum ou non (+81 % et +64 % par rapport a C). Les animaux WD pf presentaient la meme augmentation transitoire de l’activite de l’IAP caecale a 1 semaine que les animaux WD al (+408 % WD pf par rapport a C et +1 052 % WD al par rapport a C). En revanche, alors que les rats WD al presentaient une augmentation transitoire du passage caecal du LPS a 1 semaine (+49 % par rapport a C), celui-ci restait eleve a 6 semaines chez les animaux WD pf (+49 % a 1 et +71 % a 6 semaines par rapport a C). L’expression de md2 a 1 et 6 semaines et de myd88 a 6 semaines etait diminuee chez les rats WD pf (−80, −30 et −41 % par rapport a C, respectivement) contrairement aux rats WD al qui ne presentaient une diminution de md2 (−70 % par rapport a C) qu’a 1 semaine. Dans l’ileon, les animaux WD pf presentaient le meme profil a 1 et 6 semaines que les animaux WD al concernant l’activite de l’IAP (+537 et +329 % WD pf par rapport a C ; +1 000 et +900 % WD al par rapport a C) et l’expression de md2 (−28 et −30 % WD pf par rapport a C ; −31 et −25 %, WD al par rapport a C). Cependant et contrairement aux rats WD al , les animaux WD pf ne presentaient pas de diminution de l’expression de tlr4 mais une diminution de myd88 a 6 semaines (−31 % WD pf par rapport a C). Comme dans le caecum, les animaux WD pf presentaient une augmentation du passage ileal de LPS a 1 et 6 semaines (+81 et +140 % par rapport a C) non observee chez les animaux WD al . Enfin, a 6 semaines, l’endotoxemie etait elevee uniquement chez les animaux WD pf (+1 124 % WD pf par rapport a C). Conclusion Un regime obesogene induit des alterations des mecanismes homeostatiques controlant le passage intestinal du LPS dependantes de la duree de regime et du site intestinal considere ainsi que de l’hyperphagie initiale au moment du changement de regime.
Published: 2015

12. Milk vs soybean polar lipids modulate postprandial lipemia and high-fat diet-induced adiposity in mice

Author: Marie-Caroline Michalski, Manon Lecomte, Claire Bourlieu, Gaëlle Pineau, Mathilde Claude, Alain Geloen, Fabienne Laugerette, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Subjects: milk, postprandial lipemia, émulsifiant, digestive, oral, and skin physiology, food and beverages, digestion, [SDV.MHEP.EM]Life Sciences [q-bio]/Human health and pathology/Endocrinology and metabolism, lait, lipemia, lipid, lipids (amino acids, peptides, and proteins), emulsifier, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, lipide, lipémie
Abstract: Milk vs soybean polar lipids modulate postprandial lipemia and high-fat diet-induced adiposity in mice. 12. European Nutrition Conference FENS 2015
Published: 2015

13. Postprandial Endotoxemia Linked With Chylomicrons and Lipopolysaccharides Handling in Obese Versus Lean Men: A Lipid Dose-Effect Trial

Author: Gaëlle Pineau, Hubert Vidal, Emmanuelle Meugnier, Martine Laville, Jocelyne Drai, Sandra Pesenti, Fabienne Laugerette, Corinne Malpuech-Brugère, Cécile Vors, Marie-Caroline Michalski, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RH), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-CHU Saint-Etienne-Hospices Civils de Lyon (HCL)-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Centre National Interprofessionnel de l'Economie Laitiere (CNIEL), INRA, Ezus-Lyon1, Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Lipopolysaccharides, Male, Blood Glucose, Endocrinology, Diabetes and Metabolism, [SDV]Life Sciences [q-bio], Clinical Biochemistry, Biochemistry, Intestinal absorption, Body Mass Index, Endocrinology, Membrane Glycoproteins/blood, Chylomicrons, Insulin, ComputingMilieux_MISCELLANEOUS, 2. Zero hunger, Carrier Proteins/blood, Membrane Glycoproteins, Cross-Over Studies, Lipopolysaccharides/*blood, Postprandial Period, Obesity/*blood, 3. Good health, Postprandial, Endotoxemia/*blood, lipids (amino acids, peptides, and proteins), medicine.symptom, Drug, Adult, medicine.medical_specialty, Context (language use), Inflammation, Dose-Response Relationship, Postprandial Period/*physiology, Internal medicine, Diabetes mellitus, medicine, Humans, Obesity, Chylomicrons/*blood, Dose-Response Relationship, Drug, business.industry, Biochemistry (medical), medicine.disease, Dietary Fats, Endotoxemia, business, Carrier Proteins, Insulin/blood, Body mass index, Dyslipidemia, Chylomicron, Acute-Phase Proteins
Abstract: International audience; CONTEXT: Postprandial endotoxemia is a metabolic risk factor, which has been shown to originate from the intestinal absorption of gut lipopolysaccharides (LPS) using nonphysiological high-fat tests. OBJECTIVE: This study aimed to determine whether different realistic fat amounts can modulate postprandial dynamics and handling of LPS by varying postprandial lipidemia in humans of different body mass indices. DESIGN, SETTING, AND PARTICIPANTS: In a randomized, controlled, cross-over study in nutrition research center, eight normal-weight (NW) and eight obese age-matched men, without diabetes nor dyslipidemia, ingested breakfasts containing 10 vs 40 g fat. Blood samples, leukocytes, and chylomicron-rich fractions were obtained during 8 h. Plasma and chylomicron-endotoxemia, plasma LPS transporters (LBP, sCD14) and IL-6, nuclear factor kappaB (NF-kappaB) translocation, and IL-6 gene expression of immune cells were measured. MAIN OUTCOME: The postprandial fatty acid handling after ingesting 40 g fat was previously published as primary outcome. The secondary outcomes were inflammatory ones including postprandial endotoxemia, LPS handling, and plasma markers of inflammation after ingesting 10 or 40 g fat. RESULTS: Chylomicronemia increased in all subjects according to ingested fat amount (P \textless .01), but only obese had higher postprandial endotoxemia after 40 g (P \textless .05). Obese subject chylomicrons were more enriched with LPS compared with NW (PBMI \textless .01). We observed neither NF-kappaB translocation, nor variation of IL-6 expression in leukocytes. In both groups, fat amount did not modify postprandial response of plasma IL-6. However, the area under the curve (AUC) of IL-6 in obese was higher than in NW (P \textless .05) parallel to higher fasting LPS-binding protein (LBP; P \textless .05). AUC of IL-6 was correlated with LBP (P \textless .01). CONCLUSION: Postprandial endotoxemia is modulated by ingested fat amount in obese men. LPS handling in plasma through chylomicrons and LBP seems critical in driving the acute inflammatory response. The pathophysiological importance of repeated postprandial endotoxemia excursions and their contribution to a vicious cycle of LBP-driven low-grade inflammation deserve further investigation in the nutritional management of cardio-metabolic risk prevention.
Published: 2015

14. Increasing fat content from 20 to 45 wt% in a complex diet induces lower endotoxemia in parallel with an increased number of intestinal goblet cells in mice

Author: Alain Géloën, Hubert Vidal, Gaëlle Pineau, Fabienne Laugerette, Marie-Caroline Michalski, Pascale Plaisancié, Annick Bernalier-Donadille, Monique Estienne, Bérengère Benoit, Jacques Bodennec, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Institut National de la Recherche Agronomique (INRA)
Subjects: Leptin, Lipopolysaccharides, Male, Intestines/cytology/metabolism, Dietary Fats/*administration & dosage, Endocrinology, Diabetes and Metabolism, [SDV]Life Sciences [q-bio], Adipose tissue, White adipose tissue, Mucin 2, Inbred C57BL, Weight Gain, Mice, Endocrinology, Zonula Occludens-1 Protein/metabolism, Fat-Restricted, Intestinal Mucosa, Diet, Fat-Restricted, 2. Zero hunger, Nutrition and Dietetics, food and beverages, Colon/metabolism, 3. Good health, Intestines, White/metabolism, Triglycerides/blood, Goblet Cells/*metabolism, Liver, Adipose Tissue, lipids (amino acids, peptides, and proteins), Adiponectin, Goblet Cells, Adiponectin/blood, medicine.symptom, Liver/metabolism, medicine.medical_specialty, Colon, Adipose Tissue, White, Dietary lipid, Adipokine, Inflammation, Biology, Diet, High-Fat, Occludin, Internal medicine, medicine, Animals, Occludin/metabolism, Triglycerides, Interleukin-6, Inflammation/*physiopathology, Dietary Fats, Endotoxemia, Diet, Mice, Inbred C57BL, High-Fat, Leptin/blood, Immunology, Zonula Occludens-1 Protein, Interleukin-6/blood, Endotoxemia/*physiopathology
Abstract: International audience; The impacts of high-fat diets (HFDs) on the onset of metabolic endotoxemia and low-grade inflammation are well established in rodent models. However, the dose-effect of dietary lipid intakes on these parameters is not known. We hypothesized that increasing dietary lipid amounts could be linked to parallel increases of endotoxemia, low-grade inflammation, and metabolic and intestinal alterations. Six-week-old male C57BL/6J mice were fed a low-fat diet (LFD, 2.6 wt% of lipids), a moderate HFD (mHFD, 22 wt% of lipids), or a very HFD (vHFD, 45 wt% of lipids) formulated mainly using chow ingredients and milk fat. After 12 weeks, white adipose tissues, liver, intestine, distal colon contents, and plasma were collected. Only vHFD mice significantly increased body weight and fat mass vs LFD mice. This was associated with increases of plasma concentrations of triglycerides, leptin and adiponectin, and liver lipids. No such differences were observed between LFD and mHFD mice. However, mHFD developed metabolic endotoxemia and inflammation, unlike vHFD mice. In turn, vHFD mice showed more goblet cells in all intestine segments vs both other groups and a decrease of Bacteroides-Prevotella in their microbiota vs LFD mice. Finally, mHFD mice colon exhibited a decrease in lactobacilli and in the levels of occludin phosphorylation. Altogether, using complex HFD, no associations were observed between dietary lipid amounts and the magnitude of endotoxemia, inflammation, and physiological alterations developed. These results reveal the impact of the diet composition on intestinal goblet cells and mucus coat, bringing new insights about further consequences on HFD-induced metabolic disorders.
Published: 2015

15. Milk vs soybean polar lipids as emulsifier: faster postprandial lipid metabolism through greater emulsion lipolysis

Author: Manon Lecomte, Claire Bourlieu, David Cheillan, Alain Geloen, Fabienne Laugerette, Marie-Caroline Michalski, ProdInra, Migration, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA)
Subjects: [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, ComputingMilieux_MISCELLANEOUS
Abstract: International audience
Published: 2015

16. Impact de différents lipides polaires alimentaires sur la lipolyse digestive in vitro et l’absorption lipidique intestinale chez la souris

Author: Gaëlle Pineau, Manon Lecomte, Alain Géloën, Claire Bourlieu, Fabienne Laugerette, Mathilde Claude, Marie-Caroline Michalski, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL)
Subjects: 2. Zero hunger, modèle animal, Endocrinology, Diabetes and Metabolism, lipolyse, 030209 endocrinology & metabolism, General Medicine, digestion, lait, 3. Good health, 03 medical and health sciences, obésité, 0302 clinical medicine, Endocrinology, nutrition, intestin, 030220 oncology & carcinogenesis, lipide polaire, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, diabète
Abstract: Objectif Les maladies metaboliques d’origine nutritionnelles telle que l’obesite et le diabete de type 2 sont caracterisees par un metabolisme des lipides perturbe incluant une hyperlipemie postprandiale. Dans ce contexte, un interet croissant s’est developpe recemment autour des effets benefiques des lipides polaires (LP), agents texturant largement utilises dans l’industrie alimentaire, notamment sur la regulation du metabolisme des lipides. L’objectif de cette etude est d’etudier l’impact de LP de differentes sources alimentaires (vegetal vs animal) sur la digestion des lipides et la lipemie postprandiale. Materiel et methode Des souris sont gavees avec 2 types d’emulsions, des gouttelettes de triglycerides (TG) recouvertes de LP de soja ou de LP laitiers ; le plasma est ensuite collecte. Les emulsions ont une interface lipidique initiale similaire (soit ∼ 24 m2/g de lipides). Parallelement, ces 2 emulsions sont digerees in vitro via un modele de digestion humaine statique (phase gastriques et intestinales de 60 min) base sur l’utilisation de fluides digestifs simules et d’enzymes digestives animales. Resultats Les LP Laitiers induisent des concentrations de TG et d’AGNE plus elevees 1 h apres le gavage. En revanche a 4 h celles-ci sont significativement moindre suggerant que la cinetique des lipoproteines riches en TG depend du type d’emulsifiants utilises. In vitro, en phase intestinale, la cinetique d’hydrolyse des TG de l’emulsion LP laitiers est plus rapide que celle de l’emulsion LP de soja. Discussion Ces premiers resultats suggerent que la qualite des lipides polaires influence la lipolyse digestive et le metabolisme postprandial des lipides.
Published: 2014

17. Complex links between dietary lipids, endogenous endotoxins and metabolic inflammation

Author: Marie-Caroline Michalski, Cécile Vors, Noël Peretti, Fabienne Laugerette, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Centre Hospitalier Lyon Sud [CHU - HCL] (CHLS), Hospices Civils de Lyon (HCL), Régulations métaboliques, nutrition et diabètes - UM55 (RMND UM55), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National de la Recherche Agronomique (INRA), Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RA), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-CHU Saint-Etienne-Hospices Civils de Lyon (HCL)-CHU Grenoble, Fabienne Laugerette acknowledges grants from Institut Benjamin Delessert and Société Française de Nutrition. Cécile Vors is a recipient of doctoral grand from INRA & CNIEL. Marie-Caroline Michalski acknowledges a grant from ALFEDIAM., Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche en Nutrition Humaine Rhône-Alpes (CRNH-RH), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Jean Monnet [Saint-Étienne] (UJM)-CHU Saint-Etienne-Hospices Civils de Lyon (HCL)-CHU Grenoble-Université Joseph Fourier - Grenoble 1 (UJF), Vericel, Evelyne, Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and Université de Lyon-Université de Lyon-CHU Grenoble-Hospices Civils de Lyon (HCL)-CHU Saint-Etienne-Université Jean Monnet - Saint-Étienne (UJM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])
Subjects: MESH: Inflammation, Metabolic inflammation, 030309 nutrition & dietetics, Endogeny, MESH: Metabolic Diseases, Biochemistry, Mice, MESH: Endotoxins, MESH: Obesity, MESH: Animals, Intestinal Mucosa, Subclinical infection, MESH: Lipid Metabolism, 0303 health sciences, General Medicine, 3. Good health, Intestines, MESH: Insulin Resistance, MESH: Dietary Fats, lipids (amino acids, peptides, and proteins), medicine.symptom, Digestion, Lipid digestion, MESH: Intestines, medicine.medical_specialty, MESH: Rats, Inflammation, Biology, 03 medical and health sciences, Insulin resistance, Metabolic Diseases, Internal medicine, medicine, Animals, Humans, Obesity, MESH: Mice, 030304 developmental biology, MESH: Humans, MESH: Metagenome, medicine.disease, Lipid Metabolism, Dietary Fats, Endotoxemia, Rats, Endotoxins, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, Endocrinology, MESH: Endotoxemia, Metagenome, Insulin Resistance, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract: International audience; Metabolic diseases such as obesity are characterized by a subclinical inflammatory state that contributes to the development of insulin resistance and atherosclerosis. Recent reports also indicate that (i) there are alterations of the intestinal microbiota in metabolic diseases and (ii) absorption of endogenous endotoxins (namely lipopolysaccharides, LPS) can occur, particularly during the digestion of lipids. The aim of the present review is to highlight recently gained knowledge regarding the links between high fat diets, lipid digestion, intestinal microbiota and metabolic endotoxemia & inflammation.
Published: 2011

18. Pasture v. standard dairy cream in high-fat diet-fed mice: improved metabolic outcomes and stronger intestinal barrier

Author: Alain Géloën, Bérengère Benoit, Hubert Vidal, Cyrille Debard, Jacques Bodennec, Olivier Cousin, Monique Estienne, Emmanuelle Loizon, Fabienne Laugerette, Pascale Plaisancié, Emmanuelle Meugnier, Marie-Caroline Michalski, Patricia Daira, Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Sodiaal-Candia RD, Institut Multidisciplinaire de Biochimie des Lipides (IMBL), Covalab-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA), Sodiaal Candia R&D, Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Sciences Po Bordeaux - Institut d'études politiques de Bordeaux (IEP Bordeaux), and Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Covalab
Subjects: Male, Panniculitis, Milk/adverse effects/chemistry/secretion, 030309 nutrition & dietetics, Conjugated linoleic acid, [SDV]Life Sciences [q-bio], Medicine (miscellaneous), Adipose tissue, White adipose tissue, Inbred C57BL, Jejunum, chemistry.chemical_compound, Mice, Random Allocation, Functional Food, Lactation, Food science, Intestinal Mucosa, Animal Husbandry, ComputingMilieux_MISCELLANEOUS, Intestinal Mucosa/immunology/metabolism/pathology/physiopathology, 2. Zero hunger, Hypertriglyceridemia, 0303 health sciences, Nutrition and Dietetics, Hypertriglyceridemia/etiology/prevention & control, Chemistry, Aerial/chemistry/growth & development, Intestinal epithelium, Poaceae/chemistry/growth & development, medicine.anatomical_structure, Milk, Liver, Adipose Tissue, Female, Cattle/*physiology, Liver/metabolism, medicine.medical_specialty, Adipose Tissue, White, High-Fat/adverse effects, Context (language use), Diet, High-Fat, Poaceae, Functional Food/analysis, Dairy Products/adverse effects/analysis, 03 medical and health sciences, Internal medicine, medicine, Panniculitis/etiology/*prevention & control, Diet/*veterinary, Animals, Obesity, 030304 developmental biology, Dietary Fats/adverse effects/analysis/metabolism/*therapeutic use, Plant Components, Aerial, Dietary Fats, Small intestine, Diet, Mice, Inbred C57BL, Endocrinology, White/immunology/metabolism/pathology, Obesity/immunology/metabolism/pathology/*physiopathology, Cattle, Dairy Products, Plant Components
Abstract: Dairy products derived from the milk of cows fed in pastures are characterised by higher amounts of conjugated linoleic acid and α-linolenic acid (ALA), and several studies have shown their ability to reduce cardiovascular risk. However, their specific metabolic effects compared with standard dairy in a high-fat diet (HFD) context remain largely unknown; this is what we determined in the present study with a focus on the metabolic and intestinal parameters. The experimental animals were fed for 12 weeks a HFD containing 20 % fat in the form of a pasture dairy cream (PDC) or a standard dairy cream (SDC). Samples of plasma, liver, white adipose tissue, duodenum, jejunum and colon were analysed. The PDC mice, despite a higher food intake, exhibited lower fat mass, plasma and hepatic TAG concentrations, and inflammation in the adipose tissue than the SDC mice. Furthermore, they exhibited a higher expression of hepaticPPARαmRNA and adipose tissue uncoupling protein 2 mRNA, suggesting an enhanced oxidative activity of the tissues. These results might be explained, in part, by the higher amounts of ALA in the PDC diet and in the liver and adipose tissue of the PDC mice. Moreover, the PDC diet was found to increase the proportions of two strategic cell populations involved in the protective function of the intestinal epithelium, namely Paneth and goblet cells in the small intestine and colon, compared with the SDC diet. In conclusion, a PDC HFD leads to improved metabolic outcomes and to a stronger gut barrier compared with a SDC HFD. This may be due, at least in part, to the protective mechanisms induced by specific lipids.
Published: 2014

19. Identification of new binding partners of the chemosensory signaling protein Gγ13 expressed in taste and olfactory sensory cells

Author: Sylvie Grall, Claire Fenech, Esmerina Tili, Fabienne Laugerette, Zhenhui Liu, Xavier Grosmaitre, Anna Wiencis, Jean-Pierre Montmayeur, Hervé Cadiou, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Ohio State University [Columbus] (OSU), Montmayeur, Jean-Pierre, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), and Ohio State University [Columbus] ( OSU )
Subjects: Taste, G protein, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, PDZ domain, Sensory system, Biology, taste bud, zo-1, gy13, pdz, olfactory sensory neurons, mpdz, gopc, lcsh:RC321-571, Cellular and Molecular Neuroscience, Taste receptor, Taste bud, medicine, GOPC, Food and Nutrition, PDZ, Original Research Article, Receptor, lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry, ZO-1, HEK 293 cells, Neurosciences, Olfactory sensory neurons, medicine.anatomical_structure, MPDZ, Neurons and Cognition, Alimentation et Nutrition, Gγ13, Neuroscience, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract: International audience; Tastant detection in the oral cavity involves selective receptors localized at the apical extremity of a subset of specialized taste bud cells called taste receptor cells (TRCs). The identification of the genes coding for the taste receptors involved in this process have greatly improved our understanding of the molecular mechanisms underlying detection. However, how these receptors signal in TRCs, and whether the components of the signaling cascades interact with each other or are organized in complexes is mostly unexplored. Here we report on the identification of three new binding partners for the mouse G protein gamma 13 subunit (Gγ13), a component of the bitter taste receptors signaling cascade. For two of these Gγ13 associated proteins, namely GOPC and MPDZ, we describe the expression in taste bud cells for the first time. Furthermore, we demonstrate by means of a yeast two-hybrid interaction assay that the C terminal PDZ binding motif of Gγ13 interacts with selected PDZ domains in these proteins. In the case of the PDZ domain-containing protein zona occludens-1 (ZO-1), a major component of the tight junction defining the boundary between the apical and baso-lateral region of TRCs, we identified the first PDZ domain as the site of strong interaction with Gγ13. This association was further confirmed by co-immunoprecipitation experiments in HEK 293 cells. In addition, we present immunohistological data supporting partial co-localization of GOPC, MPDZ, or ZO-1, and Gγ13 in taste buds cells. Finally, we extend this observation to olfactory sensory neurons (OSNs), another type of chemosensory cells known to express both ZO-1 and Gγ13. Taken together our results implicate these new interaction partners in the sub-cellular distribution of Gγ13 in olfactory and gustatory primary sensory cells.
Published: 2012

20. CLA-Enriched Diet Containing t10,c12-CLA Alters Bile Acid Homeostasis and Increases the Risk of Cholelithiasis in Mice

Author: Amaia Zabala Letona, Maria P. Portillo, Marie-Claude Monnot, Fabienne Laugerette, Hélène Poirier, Philippe Besnard, Isabelle Niot, Anne Athias, Physiologie de la Nutrition et Toxicologie (NUTox) (U866, Lipides et nutrition, équipe 7) (NUTox), Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Santé - STIC, and Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)
Subjects: Enterohepatic circulation, medicine.medical_specialty, medicine.drug_class, Linoleic acid, [SDV]Life Sciences [q-bio], Blotting, Western, Medicine (miscellaneous), 030209 endocrinology & metabolism, Cholesterol 7 alpha-hydroxylase, Polymerase Chain Reaction, Bile Acids and Salts, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Risk Factors, Cholelithiasis, Internal medicine, Hyperinsulinémie, medicine, Hyperinsulinemia, Animals, Homeostasis, 030304 developmental biology, 0303 health sciences, Nutrition and Dietetics, Bile acid, integumentary system, Cholesterol, alpha-Linolenic Acid, food and beverages, medicine.disease, Dietary Fats, Bile Salt Export Pump, Mice, Inbred C57BL, Cholesterol 7-alpha hydroxylase, Endocrinology, Metabolism, Liver, chemistry, Nutrient physiology, Female, lipids (amino acids, peptides, and proteins), Steatosis, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract: International audience; Mice fed a mixture of CLA containing t10,c12-CLA lose fat mass and develop hyperinsulinemia and hepatic steatosis due to an accumulation of TG and cholesterol. Because cholesterol is the precursor in bile acid (BA) synthesis, we investigated whether t10,c12-CLA alters BA metabolism. In Expt. 1, female C57Bl/6J mice were fed a standard diet for 28 d supplemented with a CLA mixture (1 g/100 g) or not (controls). In Expt. 2, the feeding period was reduced to 4, 6, and 10 d. In Expt. 3, mice were fed a diet supplemented with linoleic acid, c9,t11-CLA, or t10,c12-CLA (0.4 g/100 g) for 28 d. In Expt. 1, the BA pool size was greater in CLA-fed mice than in controls and the entero-hepatic circulation of BA was altered due to greater BA synthesis and ileal reclamation. This resulted from higher hepatic cholesterol 7α-hydroxylase (CYP7A1) and ileal apical sodium BA transporter expressions in CLA-fed mice. Furthermore, hepatic Na+/taurocholate co-transporting polypeptide (NTCP) (−52%) and bile salt export pump (BSEP) (−77%) protein levels were lower in CLA-fed mice than in controls, leading to a greater accumulation of BA in the plasma (+500%); also, the cholesterol saturation index and the concentration of hydrophobic BA in the bile were greater in CLA-fed mice, changes associated with the presence of cholesterol crystals. Expt. 2 suggests that CLA-mediated changes were caused by hyperinsulinemia, which occurred after 6 d of the CLA diet before NTCP and BSEP mRNA downregulation (10 d). Expt. 3 demonstrated that only t10,c12-CLA altered NTCP and BSEP mRNA levels. In conclusion, t10,c12-CLA alters BA homeostasis and increases the risk of cholelithiasis in mice.
Published: 2011

21. The gustatory pathway is involved in CD36-mediated orosensory perception of long-chain fatty acids in the mouse

Author: Philippe Besnard, Aziz Hichami, Nicolas Darcel, Dany Gaillard, Patricia Passilly-Degrace, Jean-Pierre Montmayeur, Fabienne Laugerette, Naim Akhtar Khan, Abdelghani El-Yassimi, Lipides - Nutrition - Cancer (U866) (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA), Institut de signalisation, biologie du développement et cancer (ISBDC), Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie et chimie des protéines [Lyon] (IBCP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Centre des Sciences du Goût (CSG), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Physiologie de la Nutrition et du Comportement Alimentaire (PNCA), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Lipides et signalisation cellulaire, Université de Bourgogne (UB), Lipides - Nutrition - Cancer (U866) ( LNC ), Université de Bourgogne ( UB ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon ( ENSBANA ), Institut de signalisation, biologie du développement et cancer ( ISBDC ), Université Nice Sophia Antipolis ( UNS ), Université Côte d'Azur ( UCA ) -Université Côte d'Azur ( UCA ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de biologie et chimie des protéines [Lyon] ( IBCP ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Centre des Sciences du Goût ( CSG ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -Centre National de la Recherche Scientifique ( CNRS ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Physiologie de la Nutrition et du Comportement Alimentaire ( PNCA ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Université de Bourgogne ( UB ), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Bourgogne (UB)-Ecole Nationale Supérieure de Biologie Appliquée à la Nutrition et à l'Alimentation de Dijon (ENSBANA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Etablissement National d'Enseignement Supérieur Agronomique de Dijon (ENESAD)-Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-AgroParisTech
Subjects: CD36 Antigens, medicine.medical_specialty, Taste, CD36, Linoleic acid, Palmitic Acid, Succinimides, Oleic Acids, Context (language use), Biochemistry, Calcium in biology, Linoleic Acid, Food Preferences, Mice, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, Tongue, Taste receptor, Internal medicine, Avoidance Learning, Solitary Nucleus, Genetics, medicine, Animals, Molecular Biology, Glossopharyngeal Nerve, 030304 developmental biology, 0303 health sciences, biology, [SCCO.NEUR]Cognitive science/Neuroscience, Fatty Acids, Solitary tract, Genes, fos, Taste Buds, Dietary Fats, Mice, Inbred C57BL, Endocrinology, medicine.anatomical_structure, chemistry, [ SCCO.NEUR ] Cognitive science/Neuroscience, biology.protein, Conditioning, Operant, Calcium, Chorda Tympani Nerve, 030217 neurology & neurosurgery, Biotechnology
Abstract: International audience; The sense of taste informs the body about the quality of ingested foods. Tastant-mediated signals are generated by a rise in free intracellular calcium levels ([Ca(2+)]i) in the taste bud cells and then are transferred to the gustatory area of brain via connections between the gustatory nerves (chorda tympani and glossopharyngeal nerves) and the nucleus of solitary tract in the brain stem. We have recently shown that lingual CD36 contributes to fat preference and early digestive secretions in the mouse. We show here that 1) the induction of an increase in [Ca(2+)]i by linoleic acid is CD36-dependent in taste receptor cells, 2) the spontaneous preference for or conversely conditioned aversion to linoleic acid requires intact gustatory nerves, and 3) the activation of gustatory neurons in the nucleus of the solitary tract elicited by a linoleic acid deposition on the tongue in wild-type mice cannot be reproduced in CD36-null animals. We conclude that the CD36-mediated perception of long-chain fatty acids involves the gustatory pathway, suggesting that the mouse may have a "taste" for fatty foods. This system would constitute a potential physiological advantage under conditions of food scarcity by leading the mouse to select and absorb fatty foods. However, it might also lead to a risk of obesity and associated diseases in a context of constantly abundant food.
Published: 2008

22. CD36, un sérieux jalon sur la piste du goût du gras

Author: Bruno Patris, Patricia Passilly-Degrace, Jean-Pierre Montmayeur, Fabienne Laugerette, Isabelle Niot, Philippe Besnard, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), and Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS )
Subjects: 0303 health sciences, Taste, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], 05 social sciences, General Medicine, Biology, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, 0501 psychology and cognitive sciences, 050102 behavioral science & comparative psychology, [ SDV.OT ] Life Sciences [q-bio]/Other [q-bio.OT], Humanities, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, 030304 developmental biology
Abstract: Cet article ne possède pas de résumé.
Published: 2006

Catalog

Books, media, physical & digital resources

See catalog results

Searchworks

Select search scope, currently: Articles

Catalog

books, media & more in Jio Institute collections

Articles

journal articles & other e-resources

Refine your results

22 results on '"Fabienne Laugerette"'

1. Milk Polar Lipids in a High-Fat Diet Can Prevent Body Weight Gain: Modulated Abundance of Gut Bacteria in Relation with Fecal Loss of Specific Fatty Acids

2. Acute effects of milk polar lipids on intestinal tight junction expression: Towards an impact of sphingomyelin through the regulation of IL-8 secretion?

3. Metabolic endotoxemia: a potential underlying mechanism of the relationship between dietary fat intake and risk for cognitive impairments in humans?

4. Metabolic effects in mice of cream formulation: Addition of both thickener and emulsifier does not alter lipid metabolism but modulates mucus cells and intestinal endoplasmic reticulum stress

5. Metabolic effects in mice of cream processing: Direct ultra-high-temperature process lowers high-fat-induced adipose tissue inflammation

6. Increased jejunal permeability in human obesity is revealed by a lipid challenge and is linked to inflammation and type 2 diabetes

7. Emulsifying dietary fat modulates postprandial endotoxemia associated with chylomicronemia in obese men: a pilot randomized crossover study

8. L’hyperphagie transitoire lors de la consommation d’un régime de type occidental est nécessaire au contrôle de l’endotoxémie métabolique à moyen terme chez le rat

9. Dietary emulsifiers from milk and soybean differently impact adiposity and inflammation in association with modulation of colonic goblet cells in high-fat fed mice

10. Dietary lipid emulsions and endotoxemia

11. L’hyperphagie transitoire lors de la consommation d’un régime de type occidental est nécessaire aucontrôle de l’endotoxémie métabolique à moyen terme

12. Milk vs soybean polar lipids modulate postprandial lipemia and high-fat diet-induced adiposity in mice

13. Postprandial Endotoxemia Linked With Chylomicrons and Lipopolysaccharides Handling in Obese Versus Lean Men: A Lipid Dose-Effect Trial

14. Increasing fat content from 20 to 45 wt% in a complex diet induces lower endotoxemia in parallel with an increased number of intestinal goblet cells in mice

15. Milk vs soybean polar lipids as emulsifier: faster postprandial lipid metabolism through greater emulsion lipolysis

16. Impact de différents lipides polaires alimentaires sur la lipolyse digestive in vitro et l’absorption lipidique intestinale chez la souris

17. Complex links between dietary lipids, endogenous endotoxins and metabolic inflammation

18. Pasture v. standard dairy cream in high-fat diet-fed mice: improved metabolic outcomes and stronger intestinal barrier

19. Identification of new binding partners of the chemosensory signaling protein Gγ13 expressed in taste and olfactory sensory cells

20. CLA-Enriched Diet Containing t10,c12-CLA Alters Bile Acid Homeostasis and Increases the Risk of Cholelithiasis in Mice

21. The gustatory pathway is involved in CD36-mediated orosensory perception of long-chain fatty acids in the mouse

22. CD36, un sérieux jalon sur la piste du goût du gras

Catalog

Searchworks

Select search scope, currently: Articles Catalog books, media & more in Jio Institute collections Articles journal articles & other e-resources

Search

Search Constraints

Refine your results

Search Limiters

Topic

Publication Year Range

Language

Database

22 results on '"Fabienne Laugerette"'

Search Results

Catalog

Select search scope, currently: Articles

Catalog

books, media & more in Jio Institute collections

Articles

journal articles & other e-resources