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1. Purification of PP2Ac from Bovine Heart

Author

Hue T. Tran, Tony Ferrar, Greg B. G. Moorhead, and Anne Ulke-Lemée

Subjects
Chromatography, Ion exchange, Chemistry, Protein subunit, Size-exclusion chromatography, Ethanol precipitation, Ammonium sulfate precipitation, Catalysis
Abstract

The catalytic subunit of PP2A (PP2Ac) can be purified in milligram quantities from bovine heart using ethanol precipitation, ammonium sulfate precipitation, ion exchange and size exclusion chromatography. The detailed procedure is described to purify PP2Ac over 4 d.

Published
2007

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