10 results on '"Handan Yavuz"'
Search Results
2. Molecularly imprinted cryogel membranes for mitomycin C delivery
- Author
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Kemal Çetin, Pınar Öncel, Aykut Arif Topçu, Handan Yavuz, Adil Denizli, Fen-Edebiyat Fakültesi, Cetin, Kemal -- 0000-0002-7393-7377, and TOPCU, Aykut Arif -- 0000-0002-5434-4920
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Materials science ,Bulk polymerization ,Cell Survival ,Surface Properties ,Scanning electron microscope ,Mitomycin ,Acrylic Resins ,Biomedical Engineering ,Biophysics ,Bioengineering ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Cell Line ,Polymerization ,Molecular Imprinting ,Biomaterials ,Mice ,Drug Delivery Systems ,Materials Testing ,medicine ,Animals ,Humans ,Fourier transform infrared spectroscopy ,Chromatography ,Mitomycin C ,Membranes, Artificial ,021001 nanoscience & nanotechnology ,In vitro ,0104 chemical sciences ,Drug Liberation ,Cross-Linking Reagents ,Membrane ,Drug delivery ,Cryogel Membrane ,Mitomycin C Delivery ,Adsorption ,Swelling ,medicine.symptom ,0210 nano-technology ,Porosity ,Cryogels - Abstract
WOS: 000399567500002, PubMed: 28105892, In this study, cryogel-based implantable molecularly imprinted drug delivery systems were designed for the delivery of antineoplastic agent. Mitomycin C imprinted poly(2-hydroxyethyl methacrylate-N-methacryloyl-l-glutamic acid) cryogel membranes were produced by free-radical bulk polymerization under partially frozen conditions. The membranes were characterized by swelling tests, Fourier transform infrared spectroscopy, scanning electron microscopy, surface area measurements and in vitro hemocompatibility tests. In vitro delivery studies were carried out to examine the effects of cross-linker ratio and template content. Mitomycin C imprinted cryogel membranes have megaporous structure (10-100 mu m in diameter). The cumulative release of mitomycin C was decreased with increasing cross-linking agent ratio and increased with the amount of template in the cryogel structure. The nature of transport mechanism of the mitomycin C from the membranes was non-Fickian., TUBITAK-BIDEB2211-National Ph.D. Scholarship Programme, Kemal Cetin thanks The Scientific and Technological Research Council of Turkey (TUBITAK) for the support by 'TUBITAK-BIDEB2211-National Ph.D. Scholarship Programme'.
- Published
- 2017
3. Preparation of imprinted cryogel cartridge for chiral separation of<scp>l</scp>-phenylalanine
- Author
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Adil Denizli, Semra Akgönüllü, and Handan Yavuz
- Subjects
Materials science ,Surface Properties ,Phenylalanine ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,02 engineering and technology ,01 natural sciences ,Molecular Imprinting ,Cartridge ,Adsorption ,Molecule ,Chromatography ,Osmolar Concentration ,010401 analytical chemistry ,Temperature ,Water ,Stereoisomerism ,Fast protein liquid chromatography ,General Medicine ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Kinetics ,Membrane ,Racemic mixture ,0210 nano-technology ,Selectivity ,Cryogels ,Biotechnology - Abstract
l-Phe-imprinted cryogel cartridge was prepared for the chiral separation of l-Phe. N-Methacryloyl l-phenylalanine (MAPA) was used as a functional monomer for complexing with l-Phe. The selectivity of the membranes was investigated by using d-Phe, l-Trp, and d-Trp as competitor molecules. The PHEMAPA-l-Trp membranes were 6.4, 4.3, and 5.5 times more selective for l-Phe than d-Phe, l-Trp, and d-Trp, respectively. The PHEMAPA-l-Phe cryogel cartridge was incorporated into the fast protein liquid chromatography (FPLC) equipment and was able to separate D,l-Phe racemic mixture efficiently. The PHEMAPA-l-Phe membranes were shown to be reusable many times without significant loss of the adsorption capacity.
- Published
- 2016
4. Cholesterol removal from various samples by cholesterol-imprinted monosize microsphere-embedded cryogels
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Gözde Baydemir, Handan Yavuz, Kivilcim Caktu, and Bahar Ergün
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Materials science ,Food Handling ,Composite number ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,(Hydroxyethyl)methacrylate ,Sensitivity and Specificity ,Molecular Imprinting ,chemistry.chemical_compound ,Adsorption ,Polymethacrylic Acids ,Specific surface area ,medicine ,Animals ,Humans ,Particle Size ,Fourier transform infrared spectroscopy ,Polyhydroxyethyl Methacrylate ,Chromatography ,General Medicine ,Microspheres ,Cholesterol ,Milk ,chemistry ,Tyrosine ,Cattle ,Particle size ,Swelling ,medicine.symptom ,Molecular imprinting ,Cryogels ,Biotechnology ,Nuclear chemistry - Abstract
Cholesterol-imprinted monosize poly(glycidyl methacrylate-N-methacryloyl-(L)-tyrosine methylester) microspheres were embedded into the poly(hydroxyethyl methacrylate) (PHEMA) cryogels and the resulting composite cryogel was used for the selective removal of cholesterol. Composite cryogels were characterized by swelling tests, multipoint BET apparatus, SEM, FTIR and elemental analysis studies. Specific surface area of the PHEMA cryogel was increased from 13 to 72.7 m(2)/g by embedding of microspheres. Composite cryogels removed 80% of cholesterol from homogenized milk. The maximum adsorption capacity was found as 42.7 mg/g for intestinal mimicking solution. After 20 adsorption-desorption cycles, there was no remarkable decrease in the adsorption capacity.
- Published
- 2013
5. The fabrication of nanosensor-based surface plasmon resonance for IgG detection
- Author
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Adil Denizli, Sinan Akgöl, Lokman Uzun, Emir Alper Türkoğlu, Handan Yavuz, Belirlenecek, Akgol, Sinan -- 0000-0002-8528-1854, AKGOL, Sinan -- 0000-0003-2836-7181, and Uzun, Lokman -- 0000-0002-3971-7725
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Materials science ,Biomedical Engineering ,Analytical chemistry ,Pharmaceutical Science ,Medicine (miscellaneous) ,Nanoparticle ,Biosensing Techniques ,Microscopy, Atomic Force ,Methacrylate ,Sensitivity and Specificity ,Immunoglobulin G ,Contact angle ,Hemoglobins ,chemistry.chemical_compound ,Nanosensor ,Albumins ,Lab-On-A-Chip Devices ,Spectroscopy, Fourier Transform Infrared ,Humans ,Fourier transform infrared spectroscopy ,Surface plasmon resonance ,IgG detection ,Polyhydroxyethyl Methacrylate ,biology ,General Medicine ,Solutions ,chemistry ,Triethoxysilane ,biology.protein ,Nanoparticles ,PHEMA nanoparticles ,Gold ,human immunoglobulin G ,surface plasmon resonance ,Biotechnology - Abstract
Poly(2-hydroxyethyl methacrylate)/3-(2-imidazoline-1-yl) propyl(triethoxysilane) (PHEMA/IMEO) nanoparticles were attached on surface plasmon resonance (SPR) sensor for the real-time detection of human immunoglobulin G (IgG) in human serum. The PHEMA/IMEO nanoparticles-attached SPR sensor was characterized by Fourier transform infrared spectroscopy (FTIR), atomic force microscopy (AFM), and contact angle measurements. IgG detection studies were performed using aqueous IgG solutions at different concentrations. In order to show the selectivity and specificity of the SPR sensor, competitive kinetic analyses were performed using IgG, albumin, hemoglobin in singular and competitive manner. Finally, IgG detection in human serum was carried out.
- Published
- 2012
6. Immobilized metal affinity beads for ferritin adsorption
- Author
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Handan Yavuz, Sinan Akgöl, Adil Denizli, and Mehmet Odabaşı
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Materials science ,Aqueous solution ,biology ,Ligand ,Iron ,Inorganic chemistry ,Biomedical Engineering ,Biophysics ,Bioengineering ,Iron Chelating Agents ,Microspheres ,Biomaterials ,Methacryloyl chloride ,Ferritin ,chemistry.chemical_compound ,Adsorption ,chemistry ,Specific surface area ,Ferritins ,biology.protein ,Methacrylates ,Chelation ,Suspension polymerization ,Cysteine ,Porosity - Abstract
A new metal-chelate adsorbent utilizing N-methacryloyl-(L)-cysteine methyl ester (MAC) was prepared as a metal-chelating ligand. MAC was synthesized by using methacryloyl chloride and L-cysteine methyl ester dihydrochloride. Spherical beads with an average diameter of 150-200 microm were produced by suspension polymerization of 2-hydroxyethyl methacrylate (HEMA) and MAC carried out in an aqueous dispersion medium. Then, Fe(3+) ions were chelated directly on the beads. Properties such as specific surface area, specific pore volume and ligand occupation were determined. The specific surface area of the beads was found to be 18.9 m2/g. The total pore volume was 2.8 ml/g and represented a porosity over 52%. The average pore size of the poly(HEMA-MAC) beads was 620 nm. Fe(3+)-chelated beads were used in the adsorption of ferritin from aqueous solutions. Ferritin adsorption increased with increasing ferritin concentration. The maximum ferritin adsorption capacity of the Fe(3+)-chelated poly(HEMA-MAC) beads (Fe(3+) loading 0.81 mmol/g) was found to be 3.7 mg/g at pH 4.0 in acetate buffer. The non-specific ferritin adsorption on the poly(HEMA-MAC) beads were 0.4 mg/g. Adsorption behavior of ferritin could be modelled using both the Langmuir and Freundlich isotherms. Adsorption capacity decreased with increasing ionic strength of the binding buffer. Ferritin molecules could be adsorbed and desorbed five times with these adsorbents without noticeable loss in their ferritin adsorption capacity.
- Published
- 2005
7. Cysteinylhexapeptide Attached Poly(2-Hydroxyethyl Methacrylate) Beads for Cd(II) Removal from Human Plasma in a Packed-Bed Column
- Author
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Cigˇdem Arpa, Ömer Genç, Sema Bektaş, Adil Denizli, and Handan Yavuz
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Packed bed ,Cadmium ,Process Chemistry and Technology ,General Chemical Engineering ,Ethylene glycol dimethacrylate ,chemistry.chemical_element ,Filtration and Separation ,General Chemistry ,(Hydroxyethyl)methacrylate ,2-Hydroxyethyl Methacrylate ,chemistry.chemical_compound ,Adsorption ,chemistry ,Human plasma ,Polymer chemistry ,Chemical stability - Abstract
Poly(hydroxyethyl methacrylate) (PHEMA) beads (in the size range of 150–200 μm) with good mechanical properties were prepared and crosslinked with ethylene glycol dimethacrylate (EGDMA) to increase their chemical stability. Because of their hydroxyl groups, they can serve as affinity adsorbent and can be employed for medical applications. Cibacron Blue F3GA was succesfully immobilized onto the beads. The maximum dye attachment was 16.5 μmol/g. Then, metallopeptide-ligand cysteinylhexapeptide (CysHP) was incorporated onto these beads and they were used for removal of cadmium ions [Cd(II)] from human plasma in a packed-bed column. The maximum amount of CysHP attached was 3.2 mg/g. Non specific Cd(II) adsorption from human plasma on the PHEMA beads was 0.32 mg/g. The adsorption capacity of the beads decreased from 11.8 to 3.7 mg/g with the raise of the flow-rate from 1.0 to 5.0 ml/min. It has been found that the CysHP loading has a great effect on the capacity of beads for adsorbing Cd(II) ions from human pl...
- Published
- 2003
8. Immunoaffinity beads for selective removal of cholesterol from human plasma
- Author
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Adil Denizli and Handan Yavuz
- Subjects
Materials science ,Biomedical Engineering ,Biophysics ,Biocompatible Materials ,Bioengineering ,Antibodies ,Biomaterials ,chemistry.chemical_compound ,Adsorption ,medicine ,Humans ,Platelet ,Immunosorbent Techniques ,Polyhydroxyethyl Methacrylate ,Chromatography ,biology ,Cholesterol ,Cholesterol binding ,Membranes, Artificial ,Microspheres ,chemistry ,biology.protein ,Suspension polymerization ,Antibody ,Swelling ,medicine.symptom ,Lipoprotein - Abstract
Anti-low density lipoprotein antibody (anti-LDL antibody) attached poly(2-hydroxyethyl methacrylate-methacryloylamidophenylalanine) (poly(HEMA-MAPA)) beads were prepared for selective removal of cholesterol from hypercholesterolemic human plasma. Poly(HEMA-MAPA) beads were produced by a modified suspension polymerization and then characterized by swelling tests and SEM. Blood-compatibility tests were also investigated. The water swelling ratio of the poly(HEMA-MAPA) beads increased significantly (68%) compared with pHEMA (55%). All the clotting times increased when compared with poly(HEMA) beads. Loss of platelets and leukocytes was very low. The maximum anti-LDL antibody attachment was achieved at pH 7.0. Attachment of anti-LDL antibody was 29.6 mg/g. There was a very low non-specific cholesterol binding onto the poly(HEMA-MAPA) beads, about 0.74 mg/g. Anti-LDL antibody attached beads adsorbed in the range of 13.3-16.0 mg cholesterol/g from hypercholesterolemic human plasma. Up to 92% of the adsorbed LDL was desorbed. The binding-elution cycle was repeated 10 times using the same beads. There was no significant loss of binding capacity.
- Published
- 2003
9. Affinity separation of plasma proteins using a newly synthesized methacrylamidoalanine incorporated porous pHEMA membranes
- Author
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Yakup Arica, Adil Denizli, Rıdvan Say, Süleyman Patir, Handan Yavuz, Kırıkkale Üniversitesi, Anadolu Üniversitesi, Fen Fakültesi, Fizik Bölümü, and Say, Rıdvan
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Chemistry ,Process Chemistry and Technology ,General Chemical Engineering ,Metal ions in aqueous solution ,Analytical chemistry ,Azobisisobutyronitrile ,Filtration and Separation ,General Chemistry ,Nuclear magnetic resonance spectroscopy ,Human serum albumin ,body regions ,chemistry.chemical_compound ,albumin adsorption ,Membrane ,Photopolymer ,Adsorption ,poly(HEMA) ,amino acid membranes ,metal chelates ,medicine ,Chelation ,Nuclear chemistry ,medicine.drug - Abstract
WOS: 000176800400005, In this study, we synthesized a novel adsorbent to obtain high protein-adsorption capacity utilizing 2-methacrylamidoalanine (MAAL) containing membrane. Amino acid-ligand MAAL was synthesized by using methacrylochloride and alanine. Then, poly(2-hydroxyethylmethacrylate-co-2-methacrylamidoalanine) [p(HEMA-co-MAAL)] membranes were prepared by UV-initiated photopolymerization of HEMA and MAAL in the presence of an initiator (azobisisobutyronitrile, AIBN). Synthesized MAAL was characterized by nuclear magnetic resonance spectroscopy. p(HEMA-co-MAAL) membranes were characterized by swelling studies, porosimeter, scanning electron microscopy, Fourier transform-infra red spectroscopy, and elemental analysis. These membranes have macropores in the size range 5-10 mum. Different metal ions including Zn(II), Ni(II), Co(II), and Cu(II) were chelated on these membranes. p(HEMA-co-MAAL) were used in the adsorption of human serum albumin (HSA) from aqueous media containing different amounts of albumin (0.1-5.0 mg L-1) and at different pH values (4.0-8.0). The maximum HSA adsorption was observed at pH 5.0. The nonspecific adsorption of HSA on the pHEMA membranes was negligible 0.9 mug cm(-2). MAAL incorporation significantly increased the HSA adsorption (1.76 mg cm(-2)). The HSA adsorption capacities of the metal-incorporated membranes were Greater than that of the p(HEMA-co-MAAL) membranes under the same conditions. Higher HSA adsorption capacity was observed from the human plasma (2.88 mg HSA cm(-2)).
- Published
- 2002
10. Bilirubin removal from human plasma by dye affinity microporous hollow fibers
- Author
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Fatma Denizli, Adil Denizli, Handan Yavuz, and Serap Şenel
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Chromatography ,Bilirubin ,Process Chemistry and Technology ,General Chemical Engineering ,Sodium ,chemistry.chemical_element ,Filtration and Separation ,General Chemistry ,Microporous material ,chemistry.chemical_compound ,Membrane ,Adsorption ,chemistry ,Covalent bond ,Polyamide ,Sodium carbonate - Abstract
Bioaffinity adsorption has a unique and powerful role as a support tool in the removal of toxic substances from human plasma. Synthetic hollow-fiber membranes have advantages as support matrices in comparison to conventional hemoperfusion columns because they are not compressible and they eliminate internal diffusion limitations. In this study, Cibacron Blue F3GA was covalently attached onto commercially available microporous polyamide hollow-fiber membranes for bilirubin removal from hyperbilirubinemic human plasma. Different amounts of Cibacron Blue F3GA were attached on the polyamide hollow-fibers by changing the dye-attachment conditions, i.e., initial dye concentration, addition of sodium carbonate, and sodium chloride. The maximum amount of Cibacron Blue F3GA attachment was obtained at 42.5 μmol g−1 when the hollow fibers were treated with 3 M HCl for 30 min before performing the dye attachment. The nonspecific bilirubin adsorption on the unmodified polyamide hollow-fiber membranes was 0.65 mg g−1 f...
- Published
- 2002
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