1. X-ray spectroscopy and X-ray diffraction at wavelengths near theK-absorption edge of phosphorus
- Author
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Corinne Mas, Gérard Brandolin, J. Vicat, Richard Kahn, Heinrich Stuhrmann, Hugues Nury, Valérie Biou, Eva Pebay-Peyroula, Jean Marie Bois, Lionel Nauton, Peter Bösecke, Laboratoire d'enzymologie et biochimie structurales (LEBS), Centre National de la Recherche Scientifique (CNRS), Laboratoire de Mathématiques de Reims (LMR), Université de Reims Champagne-Ardenne (URCA)-Centre National de la Recherche Scientifique (CNRS), Biochimie et biophysique des systèmes intégrés (BBSI), Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Joseph Fourier - Grenoble 1 (UJF), Institut de biologie structurale (IBS - UMR 5075 ), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Université Joseph Fourier - Grenoble 1 (UJF)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)
- Subjects
Halobacterium ,Diffraction ,Nuclear and High Energy Physics ,Absorption spectroscopy ,Protein Conformation ,Carbon-Oxygen Lyases ,Neutron diffraction ,Molecular Conformation ,Analytical chemistry ,MESH: Equipment Failure Analysis ,01 natural sciences ,MESH: Carbon-Oxygen Lyases ,Crystal ,03 medical and health sciences ,MESH: Protein Conformation ,Purple Membrane ,X-Ray Diffraction ,0103 physical sciences ,[SDV.BBM.BC]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM] ,MESH: Phosphorus ,Instrumentation ,030304 developmental biology ,010302 applied physics ,MESH: Molecular Conformation ,0303 health sciences ,X-ray spectroscopy ,Radiation ,Chemistry ,MESH: X-Ray Diffraction ,Spectrometry, X-Ray Emission ,MESH: Spectrometry, X-Ray Emission ,Phosphorus ,Equipment Design ,MESH: Purple Membrane ,Equipment Failure Analysis ,Wavelength ,Crystallography ,Absorption edge ,X-ray crystallography ,MESH: Halobacterium ,MESH: Mitochondrial ADP, ATP Translocases ,Mitochondrial ADP, ATP Translocases ,MESH: Equipment Design - Abstract
International audience; Phosphorus is an abundant element in living organisms. It is traceable by its X-ray absorption spectrum which shows a strong white line at its K-edge, comparable with that observed for the L(III) edges of rare earth ions. With purple membrane, the variation of the imaginary part of the anomalous dispersion of phosphorus is found to be close to 20 anomalous electron units. Anomalous diffraction experiments at wavelengths near the K-absorption edge of phosphorus confirm this result. The spatial distribution of lipids derived from anomalous diffraction agrees with earlier results from neutron diffraction. Test experiments on single crystals of the carrier protein using 5.76 A photons gave a first low-resolution diffraction pattern. Various techniques of crystal mounting were attempted. In addition, fluorescence measurements on a solution of threonine synthase appear to hint at a change of the phosphate environment of the cofactor upon activator binding.
- Published
- 2005
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