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ATP-binding cassette (ABC) transporters, acting as cellular "pumps," facilitate solute translocation through membranes via ATP hydrolysis. Their overexpression is closely tied to multidrug resistance (MDR), a major obstacle in chemotherapy and neurological disorder treatment, hampering drug accumulation and delivery. Extensive research has delved into the intricate interplay between ABC transporter structure, function, and potential inhibition for MDR reversal. Cryo-electron microscopy has been instrumental in unveiling structural details of various MDR-causing ABC transporters, encompassing ABCB1, ABCC1, and ABCG2, as well as the recently revealed ABCC3 and ABCC4 structures. The newly obtained structural insight has deepened our understanding of substrate and drug binding, translocation mechanisms, and inhibitor interactions. Given the growing body of structural information available for human MDR transporters and their associated mechanisms, we believe it is timely to compile a comprehensive review of these transporters and compare their functional mechanisms in the context of multidrug resistance. Therefore, this review primarily focuses on the structural aspects of clinically significant human ABC transporters linked to MDR, with the aim of providing valuable insights to enhance the effectiveness of MDR reversal strategies in clinical therapies. [ABSTRACT FROM AUTHOR]

The rapid advancement of Wise Information Technology of med (WITMED) has made the integration of traditional Chinese medicine tongue diagnosis and computer technology an increasingly significant area of research. The doctor obtains patient's tongue images to make a further diagnosis. However, the tongue image may be broken during the process of collecting the tongue image. Due to the extremely complex texture of the tongue and significant individual differences, existing methods fail to fully obtain sufficient feature information, which result in inaccurate inpainted tongue images. To address this problem, we propose a recurrent tongue image inpainting algorithm based on multi-scale feature fusion called Multi-Scale Fusion Module and Recurrent Attention Mechanism Network (MSFM-RAM-Net). We first propose Multi-Scale Fusion Module (MSFM), which preserves the feature information of tongue images at different scales and enhances the consistency between structures. To simultaneously accelerate the inpainting process and enhance the quality of the inpainted results, Recurrent Attention Mechanism (RAM) is proposed. RAM focuses the network's attention on important areas and uses known information to gradually inpaint image, which can avoid redundant feature information and the problem of texture confusion caused by large missing areas. Finally, we establish a tongue image dataset and use this dataset to qualitatively and quantitatively evaluate the MSFM-RAM-Net. The results shows that the MSFM-RAM-Net has a better effect on tongue image inpainting, with PSNR and SSIM increasing by 2.1% and 3.3%, respectively. [ABSTRACT FROM AUTHOR]

Exhaled human breath analysis has great potential for the diagnosis of diseases in non-invasive way. The 13C-Urea breath test for the diagnosis of Helicobacter pylori infection indicates the ammonia concentration of 50–400 ppb in the breath. This work successfully developed a surface acoustic wave (SAW) resonator based on graphene/polypyrrole composite films decorated by gold nanoparticles (AuNPs–G/PPy) with sensitivity and selectivity to detect ammonia in parts-per-billion concentrations, which is promising for the accurate diagnosis of H. pylori infection. XRD, EDS, and SEM characterized the AuNPs–G/PPy nanocomposites, providing comprehensive insights into their structural, compositional, and morphological properties. The gas-sensing capabilities of the fabricated SAW sensors were extensively investigated, focusing on their response to NH3 gas at ambient temperature. The concentration of ammonia gas was effectively quantified by monitoring the frequency shift of the SAW device. Notably, our developed SAW sensor demonstrated outstanding sensitivity, selectivity, repeatability, and reproducibility for 50–1000 ppb NH3 in dry air. The excellent sensing performance of the AuNPs–G/PPy hybrid composite film can be attributed to the synergistic effects of graphene's superior conductivity, the catalytic properties of gold nanoparticles, and the conductivity sensitization facilitated by electron-hole recombination on the polypyrrole surface. [ABSTRACT FROM AUTHOR]

Abnormal electricity consumption behavior not only affects the safety of power supply but also damages the infrastructure of the power system, posing a threat to the secure and stable operation of the grid. Predicting future electricity consumption plays a crucial role in resource management in the energy sector. Analyzing historical electricity consumption data is essential for improving the energy service capabilities of end-users. To forecast user energy consumption, this paper proposes a method that combines adaptive noise-assisted complete ensemble empirical mode decomposition (CEEMDAN) with long short-term memory (LSTM) networks. Firstly, considering the challenge of directly applying prediction models to non-stationary and nonlinear user electricity consumption data, the adaptive noise-assisted complete ensemble empirical mode decomposition algorithm is used to decompose the signal into trend components, periodic components, and random components. Then, based on the CEEMDAN decomposition, an LSTM prediction sub-model is constructed to forecast the overall electricity consumption by using an overlaying approach. Finally, through multiple comparative experiments, the effectiveness of the CEEMDAN-LSTM method is demonstrated, showing its ability to explore hidden temporal relationships and achieve smaller prediction errors. [ABSTRACT FROM AUTHOR]

Simple Summary: Sodium butyrate is an important nutritional additive for livestock animals and its potential for improving growth performance is of great interest. The present study reported the optimal sodium butyrate supplementation level in milk for preweaning dairy calves and the effects of sodium butyrate supplementation in milk on the growth performance and intestinal microbiota of calves around the newborn stage and provided evidence of the effects of sodium butyrate supplementation in milk for dairy calf feeding. The aim of the present study was to investigate the effects of sodium butyrate (SB) supplementation on the growth and intestinal microbiota of preweaning dairy calves. Eighty newborn Holstein calves (56 female and 24 male) were randomly allocated to four treatment groups with 20 calves each (14 female and 6 male). The suckling milk for the four treatments was supplemented with 0, 4.4, 8.8, or 17.6 g/d SB. During the 6-week experiment, dry matter intake was recorded daily, body weight was measured weekly, and rectal fecal samples were collected in the 2nd week. The V3–V4 hypervariable regions of the microbial 16S rRNA were amplified and then sequenced. SB supplementation elevated average daily gains (ADGs) in the first and second weeks. The optimal SB supplementation level for the whole preweaning period was 8.78 g/d, as revealed by analyzing the whole preweaning period ADG using second-order polynomial regression (quadratic) equations. The alpha diversity (Shannon diversity index), beta diversity, core phyla and genera, and function of the intestinal microbiota were affected by SB supplementation. In addition, the Shannon diversity index and core phyla and genera of the intestinal microbiota were correlated with calf growth-related indices. Overall, SB supplementation in suckling milk improved the growth performance and intestinal microbiota development of dairy calves in a quadratic manner, and regression analysis indicated an optimal supplementation level of 8.78 g/d. [ABSTRACT FROM AUTHOR]

Satellite laser ranging (SLR) is the space geodetic technique with the highest degree of range, measuring precision and distances right down to the millimeter level. Thanks to the improvement of SLR station layouts and the advance of SLR technology, in recent years, more research has been conducted to determine Global Navigation Satellite System (GNSS) satellite orbits using SLR data. The primary goal of this contribution is to investigate the accuracy of BeiDou Navigation-3 (BDS-3) Satellite precise orbit determination (POD) using solely SLR data, as well as explore the impact of various factors on that accuracy. Firstly, we used actual SLR data to make the POD for BDS-3 satellites, and the POD accuracy was positively connected with the orbital arc lengths. The 9-day median root mean square (RMS) in radial (R), along-track (T), and cross-track (N) directions were estimated at 4.7–8.2, 22.1–35.2, and 27.4–43.8 cm, respectively, for comparison with WUM precise orbits. Then, we explored the impact of SLR observations and stations on POD accuracy. For 9-day orbital arc lengths, five station or 20 observation arcs may offer an orbit with a 1 m precision. Six to eight stations or 30–35 observation arcs allow an improved orbit accuracy up to approximately 0.5 m. Furthermore, we examined how measurement errors and orbit modeling errors affect the SLR-only POD accuracy using simulated SLR data. For orbital arc lengths of 9 days, each cm of random error leads to a 9.3–11.0 cm decrease in orbit accuracy. The accuracy of an orbit is reduced by 10.1–15.0 cm for every 1 cm of systematic error. Moreover, for solar radiation pressure (SRP) errors, the effect of POD accuracy is 20.5–45.1 cm, respectively. [ABSTRACT FROM AUTHOR]

The chestnut shell is usually discarded as agricultural waste and the random deposition of it can cause environmental problems. In this study, monodisperse crystalline Ag nanoparticles (AgNPs) were synthesized by a hydrothermal approach, in which the chestnut shell extract served as both reducing agent and stabilizer. The synthesized Ag nanoparticles were characterized by ultraviolet-visible (UV) spectrophotometry, transmission electron microscopy (TEM), Fourier transform infrared spectroscopy (FTIR), X-ray photoelectron spectroscopy (XPS) and X-ray diffraction (XRD) measurements. The TEM, XRD and XPS results revealed that the synthesized product was spherical Ag nanoparticles with a face-centered cubic crystal structure. The antimicrobial activity test indicated that the Ag nanoparticles modified by the chestnut shell extract had an obvious inhibitory effect on Escherichia coli, Staphylococcus aureus and Candida albicans. The measured MIC and MBC of functionalized chestnut-shell-extract AgNPs against E. coli, S. aureus and C. albicans is relatively low, which indicated that the present functionalized chestnut-shell-extract AgNPs are an efficient antimicrobial agent. [ABSTRACT FROM AUTHOR]

Chitosan (CTS) has been used as a nerve guidance conduit (NGC) material for bridging peripheral nerve defects due to its biocompatible, biodegradable, and non-toxic properties. However, the nerve regeneration effect of chitosan alone is restricted due to its inadequate biological activity. Herein, a composite, bioactive chitosan based nerve conduit, consisting of outer warp-knitted tube scaffold made from medical-grade chitosan fiber, and inner porous cross linked carboxymethyl chitosan (C-CM-CTS) sponge with radial texture was developed. The inner wall of the scaffold was coated with C-CM-CTS solution. CM-CTS provided favorable bioactivities in the composite chitosan-based nerve conduit. An in vitro study of CM-CTS revealed its satisfying biocompatibility with fibroblast and its inhibition of oxidative damage to Schwann cells. As the internal filler of the NGC, the lyophilized sponge of C-CM-CTS showed a longitudinal guidance effect for nerve reconstruction. After 10 mm defect in rat sciatic nerve was bridged with the composite bioactive chitosan-based nerve conduit, the nerve conduit was able to effectively promote axonal regeneration and played a positive role in inducing nerve regeneration and functional recovery. In addition to the functional advantages, which are equal to those of an autograft; the technology for the preparation of this conduit can be put into mass production. [ABSTRACT FROM AUTHOR]

The Tsinghua scientific satellite is a Chinese spherical micro satellite for Earth gravity and atmospheric scientific measurements. The accurate orbits of this satellite are the prerequisites to satisfy the mission objectives. A commercial off-the-shelf dual-frequency GNSS receiver is equipped on the satellite for precise orbit determination (POD). The in-flight performances of the receiver are assessed. Regular long-duration gaps up to 50 min are observed in GNSS data, and the typical data availability is about 60–70% each day. The RMS of code noises is 0.24 m and 0.30 m for C1 and P2 codes, respectively. The RMS of fitting residuals of the carrier phase geometry-free L1–L2 combination is 2.4 mm. The GNSS receiver antenna center offsets (ACOs) and antenna center variations (ACVs) maps are estimated using in-flight data for both dual-frequency and single-frequency POD. Significant improvements in POD performances are obtained when the measurement models are updated by using the ACO and ACV maps' corrections. With the updated measurement model, the RMS of the orbit overlap differences is 1.23 cm in three dimensions for dual-frequency POD, which is reduced by 27%. Meanwhile, two different empirical acceleration types are employed and compared for dual-frequency POD, and the results show that consistency on the 5 cm level is demonstrated for orbit solutions obtained with the updated measurement model. After correcting the ACO and ACV maps, the precision of single-frequency orbit solutions is better than 10 cm, which is improved by 32%. The results indicate that the antenna center modeling can significantly improve the consistency of Tsinghua scientific satellite precise orbits, which will be conducive to the realization of the mission objectives. [ABSTRACT FROM AUTHOR]

Photothermal therapy (PTT) is a promising local therapy playing an increasingly important role in tumor treatment. To maximize PTT efficacy, various near-infrared photoabsorbers have been developed. Among them, metal sulfides have attracted considerable interest due to the advantages of good stability and high photothermal conversion efficiency. However, the existing synthesis methods of metal-sulfide-based photoabsorbers suffer from the drawbacks of complicated procedures, low raw material utilization, and poor universality. Herein, we proposed a flexible, adjustable strategy capable of transforming commercial metal sulfides into injectable hydrogels for local PTT. We took copper sulfide (CuS) as a typical example, which has intense second-window near-infrared absorption (1064 nm), to systematically investigate its in vitro and in vivo characteristics. CuS hydrogel with good syringeability was synthesized by simply dispersing commercial CuS powders as photoabsorbers in alginate-Ca2+ hydrogel. This synthesis strategy exhibits the unique merits of an ultra-simple synthesizing process, 100% loading efficiency, good biocompatibility, low cost, outstanding photothermal capacity, and good universality. The in vitro experiments indicated that the hydrogel exhibits favorable photothermal heating ability, and it obviously destroyed tumor cells under 1064 nm laser irradiation. After intratumoral administration in vivo, large-sized CuS particles in the hydrogel highly efficiently accumulated in tumor tissues, and robust local PTT was realized under mild laser irradiation (0.3 W/cm2). The developed strategy for the synthesis of CuS hydrogel provides a novel way to utilize commercial metal sulfides for diverse biological applications. [ABSTRACT FROM AUTHOR]

The gas barrier property of a silicon oxide (SiOx) film synthesized from plasma-enhanced chemical vapor deposition using the tetramethysilane (TMS)-oxygen gas mixture was modified by introducing ammonia gas in the glow discharge. The change in the glow discharge with the ammonia gas incorporation was monitored by an optical emission spectrometer (OES). Structures, chemical bond configurations, and material properties of the resulting films were investigated. The introduced ammonia gas in the TMS-oxygen plasma resulted in emission lines dominated by the N2 and CN species with the suppression of the OH and oxygen-related radicals, thereby introducing nitrogen and carbon atoms in the deposited film. A silicon oxynitride (SiOxNy) film had the best surface morphology and the lowest residual internal stress was achievable by controlling the reactant gas flow ratio of the ammonia and oxygen. The barrier property to the water vapor permeation of the silicon oxide film (~1.65 g/m2/day) deposited onto the polyethylene terephthalate (PET) substrate was thus greatly improved to 0.06 g/m2/day for the film synthesized from an adequate TMS-oxygen-ammonia gas mixture. [ABSTRACT FROM AUTHOR]

The TH-2 satellite system, including the TH-2A and TH-2B, is the first distributed interferometric synthetic aperture radar (InSAR) satellite system in China. During the in-orbit operation, the TH-2A satellite should perform three maneuvers per day to keep the formation flying geometry. We estimate those maneuvers in the precise orbit determination (POD) by the GPS and BDS2 measurements on board, respectively. The residuals of the POD show that the effects caused by orbital maneuvers can be well eliminated for both the GPS and BDS2 data. The precision of the BDS2-based POD is better than 8.0 cm in the three-dimensional direction (3D) compared with the orbit derived from the GPS observations. Such a precision level of the satellite orbit satisfies the InSAR mission requirement of the TH-2. In addition, the relative error of velocity changes is employed to evaluate the maneuver estimations by the POD using the regional navigation system of BDS2. The results show that the relative error of velocity changes between the GPS- and BDS2-based POD is less than 7.0%, which indicates that the maneuver performance extracted from the regional BDS2 data is as good as that extracted from the global GPS data. In the GNSS fused processing, we found that the independent receiver clock offsets should be taken into account, since the time tag corrections for the GPS and BDS2 observations collected on the TH-2 spaceborne receivers were different. The precision of the GPS and BDS2 (GC) combined single point positioning (SPP) can be improved by 12–14% compared with the GPS-only solution when the position dilution of precision (PDOP) of GPS exceeds three. The overlap comparisons of the GC combined orbits show that the internal orbit precision of the TH-2 satellites is better than 0.7 cm. However, the improvement of the GC combined POD result is only 3–4% with respect to the GPS-only solution, which is limited to the precision of the precise orbit and clock products of BDS2 at the present stage. [ABSTRACT FROM AUTHOR]

TH-2 is China's first short-range satellite formation system used to realize interferometric synthetic aperture radar (InSAR) technology. In order to achieve the mission goal of InSAR processing, the relative orbit must be determined with high accuracy. In this study, the precise relative orbit determination (PROD) for TH-2 based on global positioning system (GPS), second-generation BeiDou navagation satellite system (BDS2), and GPS + BDS2 observations was performed. First, the performance of onboard GPS and BDS2 measurements were assessed by analyzing the available data, code multipath errors and noise levels of carrier phase observations. The differences between the National University of Defense Technology (NDT) and the Xi'an Research Institute of Surveying and Mapping (CHS) baseline solutions exhibited an RMS of 1.48 mm outside maneuver periods. The GPS-based orbit was used as a reference orbit to evaluate the BDS2-based orbit and the GPS + BDS2-based orbit. It is the first time BDS2 has been applied to the PROD of low Earth orbit (LEO) satellite formation. The results showed that the root mean square (RMS) of difference between the PROD results using GPS and BDS2 measurements in 3D components was 2.89 mm in the Asia-Pacific region. We assigned different weights to geostationary Earth orbit (GEO) satellites to illustrate the impact of GEO satellites on PROD, and the accuracy of PROD was improved to 7.08 mm with the GEO weighting strategy. Finally, relative orbits were derived from the combined GPS and BDS2 data. When BDS2 was added on the basis of GPS, the average number of visible navigation satellites from TH-2A and TH-2B improved from 7.5 to 9.5. The RMS of the difference between the GPS + BDS2-based orbit and the GPS-based orbit was about 1.2 mm in 3D. The overlap comparison results showed that the combined orbit consistencies were below 1 mm in the radial (R), along-track (T), and cross-track (N) directions. Furthermore, when BDS2 co-worked with GPS, the average of the ambiguity dilution of precision (ADOP) reduced from 0.160 cycle to 0.153 cycle, which was about a 4.4% reduction. The experimental results indicate that millimeter-level PROD results for TH-2 satellite formation can be obtained by using onboard GPS and BDS2 observations, and multi-GNSS can further improve the accuracy and reliability of PROD. [ABSTRACT FROM AUTHOR]

According to a previous geological investigation, high-speed and long-distance loess landslides in the South Jingyang platform in Shaanxi Province are closely related to the static liquefaction of loess. Considering the typical loess landslides in this area, isotropic consolidated undrained (ICU) triaxial tests and scanning electron microscopy analyses were conducted in this study. The main conclusions are as follows: (1) The stress-strain curves indicate strong strain softening under different confining pressures. The pore water pressure increases significantly and then remains at a high level; (2) The liquefaction potential index (LPI) shows an increasing trend followed by stabilization; the larger the LPI is, the smaller the state parameter (Ψ) is. The steady-state points of the loess are in the instability region; however, the steady-state strength is not zero; (3) Based on the ICU test results, the average pore diameter decreases; the shape ratio remains essentially unchanged; and the fractal dimension and roundness show different trends. The proportions of the macropore and mesopore decrease; that of the small pore increases slightly; and that of the micropore increases significantly; (4) The compression deformation of the highly spaced pores causes rapid strain hardening. A rapid strain softening results from the pore throat blockage at the beginning of particle rearrangement and reorganization. A stable strain softening is related to the agglomeration blocking of the reconstructed pore throat in the gradually stable stage of particle rearrangement and reorganization. [ABSTRACT FROM AUTHOR]

In this study, pairs of the organosilicon/silicon oxynitride (SiOxNy) barrier structures with an ultralow water vapor transmittance rate (WVTR) were consecutively prepared by the plasma-enhanced chemical vapor deposition at a low temperature of 70 °C using the tetramethylsilane (TMS) monomer and the TMS-oxygen-ammonia gas mixture, respectively. The thickness of the SiOxNy film in the barrier structure was firstly designed by optimizing its effective permeability. The WVTR was further decreased by inserting an adequate thickness of the organosilicon layer as the stress residing in the barrier structure was released accordingly. By prolonging the diffusion pathway for water vapor permeation, three-paired organosilicon/SiOxNy multilayered barrier structure with a WVTR of about 10−5 g/m2/day was achievable for meeting the requirement of the thin film encapsulation on the organic light emitting diode. [ABSTRACT FROM AUTHOR]